Construction of Bioluminescent Lactobacillus casei CJNU 0588 for Murine Whole Body Imaging

엄지은,안원경,허송,문기성 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

Recombinant DNA pLuc2 which contains a firefly luciferase cDNA from the commercial pGL3-Basic plasmid vector was constructed for bioluminescent Escherichia coli and lactobacilli. The bioluminescent signal strengths of E. coli DH5α and L. casei CJNU 0588 transformed with pLuc2 were 2.78×109 and 1.19×106 p/s, respectively. Approximately 1010 cells of bioluminescent L. casei CJNU 0588 (pLuc2) were given orally to 6-week-old female Balb/c mice. The bioluminescent signal was detected from all three mice at 1 h and one mouse at 3 h but was not detected thereafter. The signal was also confirmed from an opened abdomen and the extracted GI tract of a mouse at 1 h. From the results, we have known that L. casei CJNU 0588 pass through the murine stomach and reach the colon and cecum 1 h after oral administration.

Increased Bacteriocin Activity of a Recombinant Pediococcus acidilactici

엄지은,박재용,문기성 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

Pediocin PA-1, mainly produced from Pediococcus spp., is a well-known class IIa bacteriocin showing strong anti-listerial activity. Pediococcus acidilactici K10 producing the bacteriocin was transformed with pLR5cat(S)_PSAB in which the pediocin PA-1 structural gene (pedA) was translationally fused with a deduced signal sequence of the bifidobacterial α-amylase gene for secretion. The P. acidilactici K10 transformant presented increased bacteriocin activity when compared with that of native P. acidilactici K10. The P. acidilactici K10 mutant (K10M), in which the plasmid for pediocin PA-1 production is cured, also showed bacteriocin activity when transformed with pLR5cat(S)_PSAB, indicating that the pediocin gene was expressed in hosts. An increase in pedA gene transcription level and the detection of both transcripts from the pediocin operon and PSAB were also confirmed. The P. acidilactici K10 [pLR5cat(S)_PSAB] transformant was more inhibitory against Listeria monocytogenes than that of the P. acidilactici K10 wild type in co-cultures.

Construction of a Recombinant Leuconostoc mesenteroides CJNU 0147 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Factor

엄지은,문기성 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.6

1,4-Dihydroxy-2-naphthoic acid (DHNA), a precursor of menaquinone (vitamin K2), has an effect on growth stimulation of bifidobacteria and prevention of osteoporosis, making it a promising functional food material. Therefore, we tried to clone the menB gene encoding DHNA synthase from Leuconostoc mesenteroides CJNU 0147. Based on the genome sequence of Leu. mesenteroides ATCC 8293 (GenBank accession no., CP000414), a primer set (Leu_menBfull_F and Leu_menBfull_R) was designed for the PCR amplification of menB gene of CJNU 0147. A DNA fragment (1,190 bp), including the menB gene, was amplified, cloned into pGEM-T Easy vector, and sequenced. The deduced amino acid sequence of MenB (DHNA synthase) protein of CJNU 0147 had a 98% similarity to the corresponding protein of ATCC 8293. The menB gene was subcloned into pCW4, a lactic acid bacteria - E. coli shuttle vector, and transferred to CJNU 0147. The transcription of menB gene of CJNU 0147 (pCW4::menB) was increased, when compared with those of CJNU 0147 (pCW4) and CJNU 0147 (-). The DHNA was produced from it at a detectable level, indicating that the cloned menB gene of CJNU 0147 encoded a DHNA synthase which is responsible for the production of DHNA, resulting in an increase of bifidogenic growth stimulation activity.

하브루타를 활용한 한국어 혼합형 수업 사례 연구 - 논쟁 중심 하브루타를 중심으로 -

엄지은 한국어문화교육학회 2022 한국어문화교육 Vol.16 No.1

The purpose of this study is to present a Korean language teaching model using Habruta in a Blended learning environment and to find a teaching and learning method using it. Based on the theory, a model was designed for the ‘argument-oriented Habruta class’ for international students for academic purposes, and the class was applied in an online-offline learning environment. The effects of 'improving communication and speaking ability', 'expanding thinking ability', 'self-directed learning', and 'active interaction' were confirmed through the interview of the learners. It is significant in that it presented a class model that can induce active interaction in a Blended learning environment by presenting a Habruta class model for Korean learners for academic purposes.

포스타 전시 발표 : 혈액종양 ; 자궁내막에서 발생한 원발성 악성 흑색종 1예

엄지은,이지연,박건우,강정훈,박준호,배덕수,송상용,이세훈,김기현,김원석,정철원,박영석,임영혁,강원기,박근칠 대한내과학회 2004 대한내과학회 추계학술대회 Vol.2004 No.1

Real-world management of chronic myeloid leukemia in South Korea: the TARGET survey

엄지은,Kyoung Ha Kim,Hyewon Lee,Hawk Kim,Slader Cassandra,Inkyung Joo,Chul Won Jung 대한혈액학회 2021 Blood Research Vol.56 No.1

Background The global TARGET survey examined real-world management of chronic myeloid leukemia (CML) compared with international guideline recommendations. This report focused on the responses of physicians from South Korea compared with those of physicians from the rest of the world (ROW). Methods The self-administered, online survey, comprising 23 questions and clinical case scenarios, was completed between April and August 2017. It was designed to gather information on practicing physicians and local practices for CML diagnosis, disease monitoring, treatment, and adverse event (AE) management. Results While there were similarities in the mutation analysis and treatment efficacy between Korea and the ROW, there were also differences in CML management. Initial diagnostic testing was more comprehensive in Korea than in the ROW, and there was significantly better access to standardized polymerase chain reaction testing. Assessment of BCR-ABL levels during the first 12 months of treatment was excellent in Korea, and there was greater frontline use of second-generation BCR-ABL tyrosine kinase inhibitors. Korean physicians were significantly less likely to switch therapy for hematologic AEs. Treatment-free remission was not an important goal of therapy among Korean or ROW physicians. Conclusion This study identified some differences in the current CML management between Korea and the ROW; CML management in Korean patients was generally in line with the current guidelines.

LASSO 방법을 이용한 프라이버시 침해 우려 행태 분석

엄지은,전승환,전수영 한국자료분석학회 2019 Journal of the Korean Data Analysis Society Vol.21 No.1

As the use of various media channels increase rapidly, personal information must be filled to use various services and media contents. However, there are many problems in leakage of personal information. This study analyzes various aspects of the degree of privacy invasion concern using media panel data from 2014 to 2017. When model includes many variables, multi-collinearity and overfitting problems generally arise. This paper uses LASSO to solve these problems. For privacy invasion concern, we selected variables by LASSO and then classified the characteristics of users by decision tree analysis. The results show that SNS activity, smartphone or Wi-Fi zone usage time, and frequency of internet cafe activities have the greatest impact on personal privacy invasion. Also, men are strongly influenced by car audio, document work, and number of household members, and women are more likely to be affected by average monthly income, sleeping time, amount spent in applications, and music listening time. Samsung, LG and Apple show their own characteristics, although Samsung and LG have similar trends by year. Especially, Apple has a distinctly different trend from others. 다양한 미디어 채널 사용이 급격히 증가함과 동시에 각종 서비스와 미디어 콘텐츠를 이용하기 위해서는 개인정보를 기재해야한다. 하지만 요즈음 급증하고 있는 개인정보의 유출에 많은 문제점이 대두되고 있다. 따라서 본 연구는 2014년-2017년 기간 동안의 미디어패널 자료를 이용하여 프라이버시 침해 우려 정도에 대해 다방면으로 분석을 진행하였다. 보통 설명변수가 많을 경우 다중공선성 및 과적합 문제가 발생하게 되는데 이를 해결하기 위해 벌점화 모형 중에서 LASSO 방법이 주로 사용된다. 이에 본 논문에서는 LASSO 회귀모형으로 연도별 프라이버시 우려 정도에 미치는 변수를 선택한 후 의사결정나무 분석을 이용하여 프라이버시 침해 우려에 관한 이용자들의 특징을 분류해보았다. 분석 결과, 개인 프라이버시 침해에 대해 2014년-2017년 각각 SNS 활동, 스마트폰 이용시간, 와이파이존 이용시간, 인터넷 카페 활동 빈도가 가장 큰 영향을 주는 것으로 나타났다. 또한 성별과 핸드폰 제조사 브랜드 별로 나누어 분석해본 결과, 남성들은 카오디오, 문서작업, 가구원 수에 영향을 많이 받고, 여성들은 월평균 소득, 수면시간, 애플리케이션 관련 지출금액, 음악/음원 청취 시간에 영향을 많이 받는 것으로 나타났다. 핸드폰 브랜드별 분석 결과에서 삼성과 LG는 연도 추세를 따라가는 공통점도 보였지만 세 브랜드 모두 각자의 특징을 보였고, 특히 애플의 경우 삼성, LG와는 확연히 다른 형태를 보였다.

줄리안 반즈의 『잉글랜드, 잉글랜드』 — “멋진 브리테니아”의 허상 —

엄지은 영남대학교 인문과학연구소 2021 人文硏究 Vol.- No.94

The aim of this paper is to explore the nature of Englishness based on historical factors or authorized history in British postmodernism fictions published after the 1980s. For this, as selecting Julian Barnes’ England, England, which was published in 1998 and dealt with the issue of Englishness, I analyze how Barnes reveals the nature of Englishness with postmodernism narrative strategies in the first and the second chapter in England, England, To illustrate the fictitious and artificial nature of Englishness, in the second chapter, I focus on the function of historiographic metafiction, one of postmodernism literary forms, while examining how Pittman and his committee designs the authentic “England, England.” In the third chapter, I stress postmodernism parody as the key role in identifying not only imperfect but artificial characteristics of Englishness, and find out how parodies of political policies related to history classes, which have been controlled by the UK government since the 1980s, expose what Englishness is. In short, I will prove how Barnes defines Englishness supported by history features as a myth in England, England with the postmodern literary form and style as well as the content, and shows the possibility to expand the meaning and significant of Englishness. 본 논문은 영국성의 본질을 분석하고, 현대 사회에서 영국성의 해석 영역을 확장하는 데 목적이 있다. 1980년대 이후 영국 정부는 역사를 기반으로 한 영국성의 강화를 정책으로 지원했다. 하지만 동시대의 영국 포스트모더니즘 소설가들은 해당 개념을 지배 이데올로기의 산물로 간주하고, 다양한 의문을 제기하며 그 허구적 본질을 폭로했다. 본 논문은 줄리안 반즈가 『잉글랜드, 잉글랜드』에서 내용뿐 아니라 형식을 통해 드러내는 역사와 역사에 기반을 둔 영국성의 본질을 분석하고자 한다. 이를 위해 2장에서는 반즈가 포스트모더니즘 형식 중 하나인 히스토리오그래픽 메타픽션을 사용하여 어떻게 효과적으로 역사의 허구적 특성을 드러내는지를 분석하고자 한다. 그리고 3장에서는 반즈가 작품 전반에 걸쳐 독자들이 영국성의 본질을 직시하고, 단순히 영국성 뿐 아니라 국가 정체성을 포함한 모든 절대 가치의 본질을 재평가하도록 이끌기 위해 사용한 포스트모더니즘 패러디가 드러내는 영국성의 본질을 탐색하고자 한다. 즉, 이 연구는 현대 영국성의 본질이 신화에 불과하다는 점을 밝힘으로써 기존의 제국주의와 탈식민주의와 연관지어 분석되던 영국성의 의미뿐 아니라, 해당 개념의 탐색을 통해 기대할 수 있는 인식 확장의 가능성을 함께 제시하고자 한다.

PR 공간이 결합된 복합사옥 설계에 관한 연구 - 스포츠브랜드 사옥을 중심으로 -

엄지은 ( Eom Jieun ),신은기 ( Cinn Eungee ) 한국공간디자인학회 2022 한국공간디자인학회논문집 Vol.17 No.7

(연구배경 및 목적) 대중이 브랜드와 소통하고 경험하는 공간은 상품을 전시하고 판매하는 공간에서 멀티 문화 공간으로, 최근에는 다양한 디자인 컨셉과 체험 공간으로 확대되고 있다. 직원과 소비자 소통의 중요성은 계속 강조되어 왔으며 다양한 계획안이 제안되었지만, 상품의 전시, 판매나 소비자로의 전달성에 집중되어 있을 뿐 직원과 소비자의 접점의 공간은 구체적인 전략이 구성되어있지 않았다. 또한 선행사례의 경우 직원공간과 소비자의 공간이 철저히 분리되어 있거나 소극적인 소통을 제안하였고, 적극적인 연계는 예약을 통한 방문으로 제한적인 것이 특징이었다. 이에 본 연구는 소비자 경험 공간을 확대하고 직원과 소통하면서 직원공간의 보안, 효율성을 유지할 수 있는 복합화 전략을 제안하고자 한다. (연구방법) 본 연구는 선행연구를 통해 PR공간에 대한 변화와 유형을 알아보고 구체적인 공간 전략을 도출했다. 또한 기업 사옥과 PR 공간이 복합화된 최근 10년 사이 건축된 사례를 도심, 교외형으로 분리해 분석하여 공간의 운영방식, 프로그램의 조닝 및 동선, 인지성을 알아보고 서로 다른 사용자 그룹인 직원과 대중을 위한 복합화 전략을 도출했다. 대중의 접근성이 우수하고 수직 조닝에 기반한 도심형 사례의 전략을 토대로 동선 및 프로그램 배치 계획이 이루어졌다. (결과) 대중의 경험 기반 소비가 큰 비중을 차지하며 체험 마케팅의 범위가 다양한 스포츠 용품 브랜드의 사옥을 대상으로 하여 PR공간이 복합화된 사옥 건축을 제안하였다. PR 공간이 복합화된 6개의 사옥 분석을 통해 프로그램을 선정했으며 직원과의 적극적인 소통과 공간의 효율성을 위해 직원 복지 공간을 복합화했다. 이를 목적성과 개방성에 따라 재배치하고 용도의 복합화와 아트리움을 통해 수직, 수평적으로 공유하도록 제안했다. 가장 개방적인 외부 진입광장부터 폐쇄적인 업무공간까지 직원과 소비자의 공간의 적절한 분리와 연계를 제안했다. (결론) 본 연구는 직원 공간의 보안성과 업무공간의 공간 효율성을 유지하면서, 직원과 잠재적 소비자인 대중이 자유롭게 소통하며 직간접적인 브랜드 경험을 할 수 있는 체험 공간을 제안하고자 하였다. 또한 점차 복합화되어 가는 사옥 건축의 방향을 제안하는데도 의의가 있다. 다만 이번 연구는 조닝 계획에 초점을 맞추었기 때문에 세부 프로그램이 작동할 수 있는 공간 디자인 연구가 보완될 필요가 있다. (Background and Purpose) The space where the public communicates and experiences with brands is expanding from a space where products are displayed and sold to a multicultural space, and recently, it is expanding to a variety of design concepts and experience spaces. The importance of communication between employees and consumers has continued to be emphasized, and various plans have been proposed, but the space of contact between employees and consumers is not composed of specific strategies. In addition, in the case of precedents, the employee space and the consumer space were thoroughly separated or passive communication was proposed, and active linkage was characterized by limited visits through reservations. Therefore, this study aims to propose a complex strategy that can expand the consumer experience space and maintain the security and efficiency of the employee space while communicating with the employees. (Method) This study investigated the changes and types of PR space through previous studies and derived specific spatial strategies. In addition, by analyzing cases built over the past 10 years in which corporate offices and PR spaces have been combined, the operation method of the space, zoning and circulation of programs, and cognitive strategies were derived for employees and the public, which are different user groups. Plans for the placement of routes and programs were made based on the strategy of urban cases based on excellent public accessibility and vertical zoning. (Results) This study targets the office buildings of sports goods brands with a wide range of experience marketing, with the public's experience-based consumption accounting for a large proportion. The program was selected through analysis of 6 office buildings that combine PR spaces, and the employee welfare space was combined for active communication with employees and space efficiency. It was proposed to relocate this according to purpose and openness, and to share it vertically and horizontally through complexity of use and atrium. From the most open external entry plaza to the closed office, we proposed proper separation and linkage of the spaces of employees and consumers. (Conclusions) This study attempted to propose an experience space where employees and the public, who are potential consumers, can freely communicate and experience direct and indirect brand experiences while maintaining the security of employee space and space efficiency of the office. Research on the complex space of consumers and employees needs to be conducted in planning PR spaces combined with offices while proposing various spaces to communicate with the public in the future. However, since this study focused on the zoning plan, the detailed design for each program needs to be supplemented.

자궁내막에서 발생한 원발성 악성 흑색종 1예

엄지은 ( Ji Eun Uhm ),임태규 ( Tae Kyu Lim ),조수진 ( Soo Jin Cho ),김승태 ( Seung Tai Kim ),배덕수 ( Deok Soo Bae ),송상용 ( Sang Yong Song ),박준오 ( Joon Oh Park ) 대한내과학회 2006 대한내과학회지 Vol.70 No.5