Depigmenting activities of kojic acid derivatives

신송석,Ho Sik Rho,Heung Soo Baek,Yung Hyup Joo,Chang Seok Lee,Yong Deog Hong 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1

Biopolymer-stabilized emulsions via the electrostatic interactions of polysaccharide, protein, and glycoprotein

임형준,김도훈,신송석 한국고분자학회 2012 한국고분자학회 학술대회 연구논문 초록집 Vol.2012 No.10

아다만틸을 기반한 N-아릴아미드 신규 안드로겐 수용체 길항제

우병영 ( Byoung Young Woo ),신송석 ( Song Seok Shin ),홍용덕 ( Yong Deog Hong ),주영협 ( Yung Hyup Joo ),정연수 ( Yeonsu Jeong ),이범진 ( Beom-jin Lee ),김수동 ( Soo-dong Kim ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.1

안드로겐 수용체 길항제로서 N-아릴아미드의 신규한 아다만틸 유도체들을 합성하고 항안드로겐 활성을 평가 하였다. 아다만틸 유도체를 함유하는 N-아릴아미드는 아다만틸 치환체가 없는 유도체보다 더 높은 활성을 가졌다. 아다만틸의 공간부피 및 방향족 고리에 전자밀도 상승효과를 주는 pendant 작용기들이 강력한 길항작용에 결정적인 영향을 미쳤다. 리간드와 수용체 사이의 상호 작용을 설명하기 위해 분자 모델링 연구를 수행 하였다. A novel series of adamantyl derivatives of N-aryl amides as androgen receptor antagonists were synthesized and their anti-androgenic activities were evaluated. The N-aryl amides containing adamantyl derivatives had more activity than those lacking adamantyl substitutions. The synergistic effect of bulkiness of the adamantyl group and modulation of electron density on the aromatic ring by pendant groups was crucial to the potent antagonism. Molecular modeling studies were performed to elucidate the interactions between ligands and receptors.

Streptococcus sp. 배양 여과물(HAIRCLETM)의 모발 개선 효과

박혜림,김하연,김진영,오신석,강필선,이강혁,임정혁,신송석 사단법인 대한화장품학회 2022 대한화장품학회지 Vol.48 No.2

Streptococcus sp. is generally known as a strain that produces hyaluronic acid. In this study, we focused on ingredients other than hyaluronic acid among several metabolites produced by Streptococcus sp. during cultivation. The hair experimental sample (HAIRCLETM) was prepared with Streptococcus sp. culture filtrate under the condition that hyaluronic acid was not produced and the effect on the hair was identified. Tensile strength of hair was tested with a universal testing machine (UTM). Surface properties of a hair were examined with an atomic force microscope (AFM). Our results suggest that HAIRCLETM could prevent damage to a hair such as destroyed-cuticle, increased-faction force, and broken-hair. Furthermore, hair-related experiments confirmed that HAIRCLETM could promote the expression of hair growth factors VEGF, HGF, and Wnt10b and mitigate male hair loss by inhibiting androgen receptors and TGF-β2 expression. In addition, it was confirmed that the expression of barrier-related factors (INV, CLDN-1) was increased for oxidative stress, and the COX-2 expression, an inflammatory factor, was inhibited, thereby improving damaged scalp barriers and alleviating induced inflammation. Streptococcus sp.는 일반적으로 히알루론산을 생산하는 균주로 알려져 있다. 본 연구에서는 Streptococcus 균주가 배양하면서 만들어내는 여러 대사산물 중 히알루론산 이외의 영양성분에 주목하였다. 히알루론산이 생성되지 않은 조건에서의 Streptococcus sp. 배양 여과물로 모발 실험용 샘플(HAIRCLETM)을 제조하고 모발에 미치는효과를 확인하였다. 모발의 인장강도는 만능시험기(universal testing machine, UTM)로 측정하였으며, 모발표면의 미세구조는 atomic force microscope (AFM)으로 분석하였다. 이 연구를 통해 Streptococcus sp. 배양 여과물은 손상된 모표피개선, 마찰력증가 및 모발강도의 증가 등의 모발개선 효과가 있는 것으로 확인되었다. 또한in vitro 모발관련 실험을 통해 Streptococcus sp. 배양 여과물이 모발성장인자인 혈관내피세포 성장인자(vascular endothelial growth factor, VEGF), 간세포성장인자(hepatocyte growth factor, HGF), Wnt10b의 발현 촉진효과가 있음을 확인 하였으며, 안드로젠 수용체(androgen receptor, AR)와 TGF-β2 발현을 저해시켜 남성형탈모완화 효과도 있음을 확인 하였다. 또한 산화스트레스에 대해 장벽 관련 인자(INV, CLDN-1)의 발현을 증가시키고, 염증성 인자인 COX-2 발현을 억제시킴으로써 손상된 두피 장벽을 개선하고 유도된 염증을 완화시키는효능이 있음을 확인하였다.

The Depigmenting Activities of Hydroxyl Carboxamide Derivatives Containing Hydrophobic Moiety

조준철,노호식,주정협,안수미,원두현,신송석,박영호,서경도,박수남 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

COMPAREX 의 항염 및 진정 화장품 소재로서의 연구

김하연,이재석,이소헌,김예향,정은영,이강혁,신송석 사단법인 대한화장품학회 2023 대한화장품학회지 Vol.49 No.3

최근 다양한 외부 요인에 의해 민감해진 피부를 진정시키기 위해 더욱 안전하고 효과적인 진정 소재개발에 대한 관심이 증가하고 있다. 따라서 본 연구에서는 항염 효능으로 잘 알려진 마치현 추출물을 기반으로애엽, 홍경천 추출물을 혼합함으로써 COMPAREX를 제조하고, 피부 진정 효능에 미치는 영향을 마치현 단일추출물과 비교하였다. 실험 결과, COMPAREX는 마치현 추출물 보다 LPS에 의해 유도된 NO 생성을 더욱 억제시키고, 염증성 인자인 iNOS, COX-2, TNF-α, IL-6의 발현을 현저히 억제시킴으로써 염증완화 효능이 더 효과적인 것을 확인하였다. 또한, COMPAREX는 마치현 추출물 보다 SDS 자극에 대해 IL-1α의 유전자 및 단백질발현을 더욱 억제시킴으로써 진정 효능이 더 효과적인 것을 확인하였다. 추가적으로, 모발세포에서 COMPAREX 는 PM2.5에 의해 증가된 COX-2, IL-8의 발현을 억제시켜 두피 염증 완화 효능이 있으며, carbonylated protein 을 저해시킴으로써 외부 자극으로부터 손상된 두피를 보호할 수 있는 효능을 확인하였다. 따라서, COMPAREX는마치현 단일 추출물 보다 개선된 천연 진정 소재로의 활용 가능성을 보였고, 향후 더마 코스메틱 소재로 활용될수 있을 것으로 기대된다. Recently, there has been a growing interest in the development of safer and more effective soothing materials to calm skin that has become sensitive to various external factors. The aim of this study was to investigate the skin soothing and anti-inflammatory effects of COMPAREX compared to Portulaca Oleracea extract. The results showed that COMPAREX significantly inhibited LPS-induced NO production and the expression of inflammatory factors iNOS, COX-2, TNF-α, and IL-6 more than P. oleracea extract. In addition, COMPAREX has been confirmed to have a more effective sedative effect by further inhibiting the gene and protein expression of IL-1α against SDS stimulation than the Portulaca Oleracea extract. Furthermore, COMPAREX inhibited the expression of inflammatory factors COX-2 and IL-8 increased by PM2.5 and suppressed H2O2-mediated carbonylated protein in hair cell. These results suggest that COMPAREX has shown the potential to be used as an improved natural soothing material over P. oleracea extract, and it is expected to be used as a derma cosmetic material in the future.

Effects of Korean ginseng berry on skin antipigmentation and antiaging via FoxO3a activation

김주원,조시영,김수환,조동현,김순미,박찬웅,Takahiko Shimizu,조재열,서대방,신송석 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: The ginseng berry has various bioactivities, including antidiabetic, anticancer, antiinflammatory, and antioxidative properties. Moreover, we have revealed that the active antiaging component of the ginseng berry, syringaresinol, has the ability to stimulate longevity via gene activation. Despite the many known beneficial effects of ginseng, its effects on skin aging are poorly understood. In this study, we investigated the effects of ginseng and the ginseng berry on one of the skin aging processes, melanogenesis, and age-related pigment lipofuscin accumulation, to elucidate the mechanism of action with respect to antiaging. Methods: The human melanoma MNT1 cell line was treated with ginseng root extract, ginseng berry extract, or syringaresinol. Then, the cells were analyzed using a melanin assay, and the tyrosinase activity was estimated. The Caenorhabditis elegans wild type N2 strain was used for the life span assay to analyze the antiaging effects of the samples. A lipofuscin fluorescence assay was performed during 10 passages with the syringaresinol treatment. Results: A 7-d treatment with ginseng berry extract reduced melanin accumulation and tyrosinase activity more than ginseng root extract. These results may be due to the active compound of the ginseng berry, syringaresinol. The antimelanogenic activity was strongly coordinated with the activation of the longevity gene foxo3a. Moreover, the ginseng berry extract had more potent antiaging effects, caused a life span extension, and reduced lipofuscin accumulation. Conclusion: Taken together, our results suggest that these antimelanogenic effects and antiaging effects of ginseng berry mediate the activation of antioxidationeFoxO3a signaling.

물푸레나무로부터 분리된 Esculetin와 Esculin의 미백 효능

홍용덕 ( Yong Deog Hong ),남미희 ( Mi Hee Nam ),이창석 ( Chang Suk Lee ),신송석 ( Song Seok Shin ),박영호 ( Young Ho Park ) 대한화장품학회 2014 대한화장품학회지 Vol.40 No.1

물푸레나무 수피 추출물은 주요 생활성 물질로서 esculetin과 esculin을 포함하고 있다. 이 가운데, Esculetin은 B16F10 melanoma cells에서 IC₅₀ value 2.8 μM의 아주 좋은 melanogenesis 억제 활성을 나타내며, 이를 통해 Melan-A cell에서 melanin 합성을 감소시킨다. 더욱이, esculetin은 mushroom tyrosinase에서도 IC₅₀ value 40 μM의 억제 활성을 나타내어 melanin biosynthesis를 저해한다. 위의 결과들로서, 우리는 melanogenic enzyme 활성 조절에 의해 melanin 생성을 저해하는 효과적인 피부미백 물질로서 esculetin을 제안하고자 한다. Stem bark extracts of Fraxinus rhynchophylla Hance were found to contain two major bioactive components, esculetin and esculin. Esculetin substantially inhibited melanogenesis in B16F10 melanoma cells, with an IC₅₀ value of 2.8 μm, and reduced melanin synthesis in Melan-A cells. Moreover, esculetin suppressed melanin biosynthesis by inhibiting mushroom tyrosinase activity, with an IC₅₀ value of 40 μM. Taken together, these results suggest that esculetin could serve as an effective skin-lightening agent that inhibits melanin production by regulating the activity of melanogenic enzymes.

Effect of polysaccharides from a Korean ginseng berry on the immunosenescence of aged mice

Miseon Kim,이영수,김주원,Sang Yun Han,Su Hwan Kim,Dae Bang Seo,Jae Youl Cho,신송석 고려인삼학회 2018 Journal of Ginseng Research Vol.42 No.4

Background: Korean ginseng has been widely evaluated to treat human diseases; however, most studies on Korean ginseng have focused on its root. In this study, polysaccharides [acidic-polysaccharide-linked glycopeptide (APGP) extracted with 90% ethanol and hot water] were prepared from Korean ginseng berries, and their effect on immunosenescence was explored. Methods: The effect of APGP on thymic involution was evaluated by measuring the size of thymi dissected from aged mice. The effect of APGP on populations of immune cells, including natural killer (NK) cells, dendritic cells, age-correlated CD11c-positive B cells, and several subtypes of T cells [CD4- positive, CD8-positive, and regulatory (Treg) T cells] in the thymi and spleens of aged mice was analyzed by fluorescence-activated cell sorting analysis. Serum levels of interleukin (IL)-2 and IL-6 were evaluated by enzyme-linked immunosorbent assay analysis. Profiles of APGP components were evaluated by high-performance liquid chromatography (HPLC) analysis. Results: APGP suppressed thymic involution by increasing the weight and areas of thymi in aged mice. APGP increased the population of NK cells, but showed no effect on the population of dendritic cells in the thymi and spleens of aged mice. APGP decreased the population of age-correlated CD11c-positive B cells in the spleens of aged mice. APGP showed no effect on the populations of CD4- and CD8-positive T cells in the thymi of aged mice, whereas it increased the population of Treg cells in the spleens of aged mice. APGP further decreased the reduced serum levels of IL-2 in aged mice, but serum levels of IL-6 were not statistically changed by APGP in aged mice. Finally, HPLC analysis showed that APGP had one major peak at 15 min (a main type of polysaccharide) and a long tail up to 35 min (a mixture of a variety of types of polysaccharides). Conclusion: These results suggested that APGP exerted an anti-immunosenescent effect by suppressing thymic involution and modulating several types of immune cells.

Synthesis and Biological Evaluation of Kojic acid Derivatives as Tyrosinase Inhibitors

Yeong Jin Choi,Ho Sik Rho,Heung Soo Baek,Yong Deog Hong,Yung Hyup Joo,신송석,김지만 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.12