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Meiotic irregularities in larix
Dong Ill Shin(申東逸) 한국육종학회 1995 한국육종학회지 Vol.27 No.2
Meiotic irregularities occured during microsporogenesis in four different larch species were investigated as the first step to determine factors affecting seed yield in Larix. The major abnormalities observed in the materials examined were chromosomal stickiness, degeneration of PMCs(pollen mother cell), acentric chromosomes, unequal distribution of chromosomes, lagging chromosomes, and aberrant microspore formation. Among them degeneration of PMCs, acentric chromosomes at metaphase 1, and aberrant microspore formation were the most common irregularities in all species investigated. Conclusively, meiosis of the larch PMCs was disrupted with total frequencies ranging from 28.3% to 52%, depending on the species. The results obtained in this study indicate that pollen quality might be responsible for seed loss in Larix.
온실에서 자라는 낙엽송의 접목묘와 야외에서 자라는 실생묘로부터 (實生苗) 개화의 (開花) 유도
신동일 ( Dong Ill Shin ) 한국산림과학회 1996 한국산림과학회지 Vol.85 No.3
GA4/7 spray, injection and several cultural treatments were applied to the greenhouse-grown potted hybrid larch(Larix decidua × leptolepis) grafts and field-grown European larch seedlings to induce early flowering. A treatment consisting of repeat-ed GA4/7 sprays, alone, was the most effective flower induction treatment for greenhouse-grown, potted larch grafts. Root pruning as a adjunct treatment did not show synergistic effects. Injection for potted grafts with GA4/7 was not useful approach in this study and it resulted in increased mortality. In the field experiment with 10-Year-old larch seedlings, repeated GA4/7 sprays in combination with root pruning or with plastic mulching appears to be useful and practical means for inducing larch flowers:
신동일(Dong Ill Shin),데이비드 F . 카노스키(David F . Karnosky) 한국산림과학회 1995 한국산림과학회지 Vol.84 No.2
Various factors reducing seed yield in 4 Larix species throughout the whole reproductive cycle were investigated and partitionate losses attributed to them were determined. Pollen quality, lack of pollination, and degeneration of female gametophyte played minor roles in reducing seed yield. Failure of pollinated ovules to be fertilized was an important factor causing seed loss. Embryo degeneration was also a major factor causing seed loss in all 4 species. Strobili abortion, which causes loss of all potential seeds in a cone, was the most important factor in reducing seed loss in this study. Based on the results obtained from this studs, hybridizations in either direction between European larch and Japanese larch are likely to resulting viable seed. However, hybridization between tamarack as a mother tree and European larch are not likely to result in viable seeds being produced.
몇가지 삼림수종 (森林樹種) 종자발아에 (種子發芽) 대한 낙엽송엽 (落葉松葉) 추출물의 Allelopathic 효과
박용구,강군수,신동일 ( Young Goo Park,Goon Su Kang,Dong Ill Shin ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.1
Inhibitory effect of L. leptolepis fallen leaf extracts on the germination of Pinus densiflora, × Pinus rigitaeda, Pinus rigida, Pinus thunbergii and Larix leptolepis was investigated. Germination of those seeds in culture room and in pot at field showed the highly significant inhibitory effects by the aqueous extracts from L. leptolepis fallen leaves. Among them L. leptolepis was the most severely inhibited by the aqueous extracts used. To identify allelopathic substances, thin-layer chromatography was employed. Gallic, ferulic, t-cinnamic and vanillic acids were identified from fallen leaves of L. leptolepis. From the results, it is assumed that autotoxicity of L . leptolepis may act as inhibitory factors on germination of the species in natural stands.
식물의 초경량 조직을 이용한 미토콘드리아의 DNA와 RNA 정제
김경민,임용숙,신동일,설일환,Kim Kyung-Min,Lim Yong-Suk,Shin Dong-Ill,Sul Ill-Whan 한국생명과학회 2006 생명과학회지 Vol.16 No.2
We present a fast and simple protocol for purification of mitochondria, mitochondrial DNA, and RNA from small amounts of tomato leaves. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. Mitochondria was confirmed by MitoTracker. The mitochondrial DNA was not contaminated by plastid DNA, was successfully used for PCR. Similarly, the isolated mitochondrial RNA was not contaminated only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for RT-PCR analysis 본 실험에서는 토마토의 종자를 기내 배양하여 얻어진 1g 이하의 무균 잎 조직을 이용하여 미토콘드리아를 분리 정제하여 MitoTracker를 이용하여 세포생물학적으로 확인하였고, 이들의 mt를 이용하여 미토콘드리아 DNA와 RNA를 추출과 검정을 하였다. 또한 고농도의 이온성을 이용하여 미토콘드리아와 mtDNA 및 mtRNA을 추출할 수 있었으며, 식물의 여러 종류에도 사용되어질 수 있을 것이다. mtDNA는 PCR 분석에 의하여 plastid DNA와 혼재되어 있지 않음을 확인하였다. mtRNA는 RT-PCR 분석을 통하여 plastid RNA와 흔재되어 있지 않음을 확인할 수 있었다.
버드나무 ( Salix koreensis ) 현탁배양 (懸濁培養) 세포의 대사산물
박용구(Young Goo Park),신동일(Dong Ill Shin),이상구(Sang Goo Lee) 한국산림과학회 1989 한국산림과학회지 Vol.78 No.2
Cell suspension cultures for Salix koreensis was well established at the supplements of 2, 4-D with cytokinin particulary the combination of 1.0 ㎎/ℓ 2, 4-D with 0.1 ㎎/ℓ of zeatin. These combined rates of phytohormones are also effective to callus induction from S, koreensis leaf and its multiplication. Cultured media exhibited the great inhibitory effect on the germination of rice, barnyard grass and lettuce seeds, indicating the presence of biologically active substances in media. Several phenolic compounds such as pyrogallol, sinapic acid, cinnamic acid, tannic + gallic and p-chlorobenzoic acid were detected in the cell suspension culture. The inhibitory effect exhibited by cultured media may be partly attributed to these phenalic compounds.
종의 포플라수종에서 리그닌생합성에 관계된 OMT유전자의 발현
박용구,박희성,최장원,설일환,정일경,신동일,Park, Young-Goo,Park, Hee Sung,Choi, Jang Won,Sul, Ill Whan,Chung, Il Kyung,Shin, Dong Ill 한국생명과학회 1998 생명과학회지 Vol.8 No.4
We analyzed lignin content and wxpression of OMT gene during growth season in two hybrid poplar species. OMT gene expression was observed mainy in the developing secondary xylem where major quantity of lignin occurs. Lignin content in the xylem tissue increased as plant resumed growth in the spring and reached the highest in the late August. Change in lignin content was concurrent with that of OMT gene expression, indicating OMT is a key enzyme in lignin biosynthesis. 리그닌함량이 감소한 펄프소재의 육성을 위해 OMT유전자와 리그닌생합성 간의 관계를 2종의 포플라 수종을 이용하여 실험하였다. OMT유전자의 발현은 리그닌생합성이 가장 많이 일어나는 developing secondary xylem에서 높앗다. 이 사부조직에서의 리그닌함량의 증가는 봄에 성장을 개시할 때부터 증가하기 시작하여 8월 말 경에 가장 높았다. 이러한 리그닌의 증가는 OMT유전자의 발현 증가와 일치하였으며, 이는 OMT유전자가 리그닌생합성에서 매우 중요한 역할을 함을 나타내는 것이다.