http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
구기자나무의 잎절편체로 부터의 Callus와 부정근유도에 미치는 영향
설일환,안인숙,조현제 대구산업정보대학 2000 논문집 Vol.14 No.2
Punctured leaf segments from Lycium chinense Mill. were cultured on the 3 different types of medum (B5, MS, WPM) containing different concentrations of auxins (2,4-D and NAA). After 12 weeks in culture, high amount of white and/or yellowish colored callus and number of roots were induced successfully. Among tested three types of medium, WPM containing 10μM of 2,4-D produced calli over 2 g per plate and the same medium containing 20μM NAA produced over 20 roots per leaf segment. The leaf segments on WPM showed the best responses unlike two medium, and lower concentrations of auxins showed the best results on inducing callus and producing adventitious roots. While comparing with control, callus induction did not occur however spontaneous roots were occurred resulting from that endogenous auxin contents in lycium leaf segments are high enough to induce. Currently, large scale of lycium cell culture is proceeding under the suspension culture methods and undergoing regeneration research for genetic transforamtion of Lycium chinense Mill..
임용숙,설일환 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5
In other to produce vinegar using Schizandru chinensis Ballon(omija), acetic acid bacteria(AAB) were selected from several conventional vinegars, and total 30 acetic acid bacterial strains were isolated. Among the isolated strains, a strain was selected from medium containing omija juice which showed the highest productivity of acetic acid. The strain was identified as Acetobucter sp. C5-1b. Optimum conditions for acetic acid production of Acetobacter sp. C5-1b were involved with 30℃ of fermentation temperature and shaking culture. The acidity of culture medium was reached to 5.3% after 8 days shaking cultivation at 30℃.
이지영,이승우,김용구,정인식,설일환 慶熙大學校 1997 論文集 Vol.26 No.-
Experiments were conducted to find out the optimum cultural conditions for micro-cross sectioned leaf segments of Gypsuphila paniculata L. Gerbera hybrida and Dianthus caryophyllus. Survival percentages of micro-cross sectioned segments were the highest in gypsophila, followed by carnation. Gerbera showed on survived segments. In gypsophila, segments thicker than 400㎛ were required to obtain over 75% survival. The percentage of adventitious bud formation was much higher with 600㎛ segment as compared with 400㎛. The regeneration of micro-cross sectioned segments of gypsophila was not sensitively affected by the auxins at varying concentrations. The elongation of adventitious shoot was promoted by kinetin compared with BA. In segments of 600㎛ thickness, adventitious shoot formation was promoted by BA compared with kinetin Adventitious shoot formation or shoot growth was not affected by KNO_3 concentrations with the segments of 400㎛ thickness. But adventitious shoot elongation was promoted significantly with segments in 600㎛ thickness. KH_2PO_4 (170 mg/1, standard concentration of MS medium) was effective for adventitious shoot formation. MgSO_4 also promoted adventitious shoot formation myo-Inositol tended to be suppressed regeneration of gypsophila.
배지조성에 따른 포플라 Callus의 Anthocyanin 생산
방혜진,이승우,최근원,정인식,설일환 慶熙大學校 1997 論文集 Vol.26 No.-
Studies were carried out to investigate the effect of cultural media on callus growth and anthocyanin production using the callus derived from the leaf discs of the Populus hybrid (Populusnigra × Populus maximowiczii cv. Yanghwangchul). Callus growth was the best in SH medium supplemented with 30 g·liter^-1 sucrose whereas anthocyanin production was maximum in White medium. But the amount of anthocyanin biosynthesis per vessel was the best in SH medium. Callus growth was similar in SH medium containing sucrose, fructose of glucose, but anthocyanin biosynthesis was enhanced in SH medium containing sucrose. Sucrose concentrations of 3.5% was the best for both callus growth and total amount of anthocyanin biosynthesis per vessel. Maltose suppressed both callus growth and anthocyanin biosynthesis. Callus growth was enhanced by higher concentrations(2X) of KNO_3 and CuSO_4, and by standard concentrations(1X) of CaCl_2 and H_3BO_3 in the medium. Total amount of anthocyanin biosynthesis was, however, somewhat different from the pattern of salts effects on callus growth.
포플라 Callus의 배양환경에 따른 Anthocyanin 생합성
방혜진,이승우,최근원,정인식,설일환 경희대학교 생명자원과학연구원 1997 遺傳工學論文集 Vol.9 No.-
Studies were carried out to investigate the effects of various cultural and environmental conditions on callus growth and anthocyanin biosynthesis of the callus derived from the leaf discs of the Populus hybrid (Populus nigra × Populus maximowiczii cv. Yanghwangchul). Even though the largest amount of anthocyanin was obtained from the calli grown in the vessels illuminated with white fluorescent light, no significant differences in callus growth could be obtained among other vessels illuminated with different light qualities, i.e., red, yellow, blue, green and dark. Callus growth and anthocyanin biosynthesis were promoted by long-day conditions (16-hour photoperiod or longer). The calli grown in Schenk & Hildebrandt (SH) medium continuously (SH→SH) showed the best growth whereas the highest amount of anthocyanin biosynthesis was obtained in the calli grown in SH→SH or SH→White media.
Environmental Control for Greenhouse Management
Sul, Ill-Whan,Shin, Dong-Ill 대구효성가톨릭대학교 응용과학연구소 1998 응용과학연구논문집 Vol.6 No.2
온실재배는 겨울이 긴 우리나라의 기후특성과 보다 높은 소득의 창출, 그리고 식물의 생리 등 학문적 연구목적을 위해 많이 이용되고 있다. 그러나 자본집약적이고 높은 관리기술이 요구되는 이러한 시설재배를 위해서는 시설의 표준화, 시설내의 환경관리와 재배방법의 체계화가 필요하다. 시설내의 재배환경관리에 있어 가장 중요한 요인인 heating,과 cooling은 지역의 기후특성, 온실의 형태 및 크기 등을 고려하여 최소의 경비로써 효율이 높은 최적의 system,을 찾는 것이 중요하다. 본 총설에서는 현재 이용되고 있는 heating 과 cooling system에 대한 장,단점을 기술하고자 한다.
Construction of cDNA and Genomic Library From Sequoia Sempervirens
Sul, Ill-Whan 대구산업정보대학 2001 논문집 Vol.15 No.-
분자생물학적으로 세포 competence를 규명하기 위한 첫 번째 단계로서 cDNA library와 genomic library를 구축하였다. 고도로 정제된 genomic DNA를 분리하여 Sau3A 제한효소로 처리한 후 density gradient로 원심하여 약 5에서 20kb의 DNA 단편들을 분리ㆍ정제하여 EMBL3 phage에 package하였다. 이들은 다시 E.coli LE392에 transfection 시킨 후 phage를 증폭시켰다. 이들 genomic library의 titer를 검증한 결과 원액의 phage 추출물 1ml 당 4.2×10^(6)으로 상당히 좋은 library를 구축하였다. 동시에 고도로 정제된 total RNA에서 cDNA library를 구축하였다. 약 4g의 세콰이아 엽육조직에서 분해되지 않는 약500에서 700㎍의 total RNA를 성공리에 분리하였다, 이들 RNA를 사용하여 약 0.5㎍의 mRNA를 분리 하였으며, 이들 RNA를 사용하여 cDNA를 합성하였다. 이들은 다시 pBluescriptⅡ(KS+)에 ligatin 시킨 후 E.coli에 형질전환시켰다. 효율은 4×10^(5)으로 상당히 좋은 cDNA library을 구축하였다. 현재 이들 library를 사용하여 competence에 관련된 유전자의 분리를 수행하고 있다.