사과 炭疽病菌의 培養的 性質 및 殺균 效果에 關한 硏究

申鶴均 안성산업대학교 1968 論文集 Vol.2 No.-

(1) 사과 炭疽病菌은 培養基의 生理的 性質로 몇가지 Graup으로 나눌 수 있는데 大體로 菌中菌絲의 發育이 抑制되는 것은 胞子의 形성이 많았으며 色이 紛紅色이었고 反對로 氣中ㅇ絲의 發育이 良好한 것은 胞子의 形成이 적고 色도 灰一灰綠色이었다. (2) 고추, 포도, 사과 및 참빗살에서의 分離菌은 사과 炭疽病菌인 Glomerella Cingulate라고 할 수 있으며 감, 참외 및 복숭아 分離菌은 各各 다른 病原性을 나타내며 別種이 炭疽病菌이 아닌가 생각된다. (3) 사과 炭疽病에 대하여 가장 效果的인 殺菌劑는 Difolatan, Phaltan및 Ditane M-45 이였었다. Monoconidial cultures of causal organism of authracnose, isolated from cight susceptible plants obtained from various places have been studied in relation to variability in culture, and in pathogenicity and to determine the yalue, if any of certain fungicdes as control for bitter not disease of apple. 1. Several groups defined from each other on nutrient media in the following colony characters; Aerial mycelial development suppressed group which greatly produced spore formition and colouring was pink. But well developed group of aerial mycelia which greatly reduced spore formation and colouring was gray to grayish green. 2. It is conceuded that the causal organism of authracnose, isolated from red pepper, grapo, apple and Euonymus alatus were Glomerella cingulta resulted from cross inoculation. But the causal organisms of authracnose, isolated from persimmon, Japanese melon and peach were not same organism of apple bitter rot disease resulted from no pithogenicity appeared. 3. Among the tested fungicides the influence of Difolatan, Phaltan, and Dithane M-45 were appeared the most remarkable.

日本腦炎에 關한 疫學的 調査 (1976)

신학균(申鶴均),이한일(李漢一),김경호(金慶浩) 대한바이러스학회 1976 Journal of Bacteriology and Virology Vol.6 No.1

A serological and an entomological sureys of Japanese encephalitis(J.E.) have been carried out from the pre-epidemic to the duration of epidemic season in 1976. Specially, emphasis were given to the seasonal prevalence of Culex tritaeniorhynchus, and the evidence for swine infection rate with J. E. virus and concerning the meteorological data of 1975 and 1976. The study areas were selected such as Busan-City, the most southeastern area, Gwangju-City, the most south-western area, and Goyang-Gun where it located at the central area of the Republic, and those were virtually covered nation-wide in terms of latitudinal and longitudinal degrees. The results obtained are summarized as follows: 1) It was found that more than 50% of hemagglutination inhibition (H-I) antibody converted among the less than 1-year old pigs in most of the study areas in the middle of September. 2) The population density of Culex tritaeniorhynchus was little lower, and seasonal duration was much shorter than those of previous years. 3) The first J.E. human laboratory confirmed case was occurred on 10th September when the H-I antibody conversion rate of swine was still remaining steadily at 30% in the study areas. In view of three important facts, the authours could predict that Japanese encephalitis cases wiil be broken out sporadicaly rather than epidemic form, and the seasonal duration of an epidemic be shorter than the previous year.

日本腦炎바이러스의 生態學的硏究 (1972)

신학균(申鶴均),정의범(鄭義範),김부영(金溥榮),송영수(宋榮守),김경호(金慶浩),이성우(李晟雨) 대한바이러스학회 1972 Journal of Bacteriology and Virology Vol.2 No.1

Through the epidemic season in 1972, studies on seasonal occurrence of Japanese Encephalitis(J. E) virus among the sentinel piglets, pigs in slaugbter houses and mosquitoes were carried out in three different localities by means of the virus isolation and antibody titration. The study was also carried out the epidemiological relationship among the human cases, mosquitoes and swine infection. The results are summarized as follows: 1. Results of Hemagglutination Inhibition (H-I) antibody titration of sentinel pigs from June to September, 1972 by areas. 1) Pusan City (Veterinary Diagnosis Labolatory in Sasang County) July, 14: Positive rate 30% (It was considered as maternal immunity) July, 21: Aug 18: Positive rate 0% Aug. 25: Positive rate 22% Sept.1: Positive rate 67% Sept. 8: Positive rate 88% 2) Kwangzu City (College of Agriculture, Univ. of Jeonnam) July, 1: Positive rate 20% July, 14: Positive rate 40% Aug. 1: Aug. 25: Positive rate 0% Sept. 1: Positive rate 60% ll. Human Cases Though the first clinicaly doubtful case of J. E was early in June but epidemic outbreak was began on Sept. 8 when sentimel pigs H-I antibody titer were risen above 90 per cent. It was 2 or 3 weeks later than that of previous year. Lll. Japanese Encephalitis virus isolation 1) Japancse Encephalitis virus isolation from sentinel pigs: J.E virus strain was isolated from on pigs blood out of ten sentinel pigs in Pusan on August 26, 1972 2) Japanese Encephalitis virus isolation from mosquitoes: One J.E virus strain was isolated from 15 pools of Culex tritaeniorkyehs(C. t) mosquitoes collected at Jeollanam-Do where the highly epidemic area of J. E, 1972. Two isolated J. E virus strains are under testing for final identification.

日本腦炎疫學調査硏究

신학균,김경호,정의범,김부영,이한일,박기덕,이온영,이성우 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

사서직 충원상의 문제점

신학균,Sin, Hak-Gyun 한국도서관협회 1969 圖協月報 Vol.10 No.4

우리나라의 C형간염 바이러스 항체 분포

신학균,윤재득,유재창,김문보,김기순,서순덕 대한바이러스학회 1993 Journal of Bacteriology and Virology Vol.23 No.2

Enzyme immuno assay (EIA) which utilizes a solid phase coated with a recombinant antigen derived from the hepatitis C virus (HCV) genome was evaluated for efficacy in the detection of anti-HCV. Using the antigen expressed on E. coli from a cDNA clone encoding structural region and non-structural region of HCV genome, the prevalence of HCV antibody on 2858 peoples in Korea was investigated. Prevalence of antibodies to HCV is 1.2% in townsmen, 0.7% in farm village residents and 2.7% in seashore residents considered to be at low risk for exposure to HCV. These limited data also indicate that the prevalence of antibodies to HCV is 31.0% in commercial plasma donors, 10.6% in HIV(Human Immunodefecience Virus) antibody positive group and 4.8% in prostitutors considered to be at high risk for exposure to HCV. And 51(45.9%) sera were positive for hepatitis B core antibody, 3(2.7% ) sera were positive for hepatitis B surface antigen and 27(24.3%) sera were elevated ALT(alanine aminotransferase) concentration among 111 sera which were confirmed anti-HCV positive. We confirmed 10 specimens have had RNA of hepatitis C virus among 25 anti-HCV positive specimens by polymerase chain reaction(PCR) and southern blot hybridization, simultaneousely, comparative test was carried out among 8 commercial anti-HCV kits in Korea, and 6 kits had shown specific homology more than 95%. These seroepidemiological data suggest that HCV plays an important role as an etiological agent in Korea.

1990년 한국에서의 위험군 및 비위험군에 대한 C형 간염 항체유병율 조사

신학균,윤재득,박근용,김문보,정연호,서순덕,용군호,이성우 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.2

We carried out serological survey to detect the prevalence of HCV antibody in risk and non-risk groups by rneans of EIA technique with Abbott diagnostic kit and ALT (Alanine transaminase) test kit in Korea. In the risk groups, the prevalence of Anti-HCV of Hemodiaslysis patients, Paid- blood donors and RIA patients were 9.5%, 34.6% and 4.81% respectively. In the non-risk groups, the HCV antibody prevalence of Cheju inhabitants and Public employees were 1.27% and 1.49% respectively and overall prevalence of non-risk group is 1.44% Especially, in Paid-blood donors, the young age group under 30 years occupied about 67.8% and prevalence of HCV antibody of this age group is as high as 49.7% while the highest rate of Anti- HCV positive is 65.9% of the 35-40 years old group. We conclude that the paid-blood donors are, first of all, the major public health problem. So there is urgent need to find out exact control measures for this group. And the non-risk group's HCV positive rate is somewhat low but epidemiologic study should be continued to verify the exact status of HCV infection in Korea.

1969년도의 공공도서관계

신학균,Sin, Hak-Gyun 한국도서관협회 1969 圖協月報 Vol.10 No.10

한국인에서 E형 간염 바이러스의 항체 유병률에 관한 조사

신학균,윤재득,정연호,김문보,서순덕,김정서 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.2

Serosurvey of hepatitis E virus (HEV) infection, known as enterically transmitted non-A, non-B hepatitis (ET-NANBH), was done in Korea between May through August, 1991 using anti HEV EIA method developed by Genelabs Inc. in 1990. The coated polypeptide antigens were expressed in Baculovirus vector from the specific HEV cDNA clones derived from two patients infected with HEV, one from Burma (antigen A) and the other from Mexico (antigen B). The 1,079 examinees was arbitrarily grouped into risk (406) and non-risk (673) group according to the serologic markers of hepatitis B virus (HBsAg, HBsAb, and HBcAb), anti-hepatitis C virus (HCV), and alanine transarninase (ALT) value but not to the risk of HEV. The overall seroprevalences of HEV in non-risk and risk group were 10.4 %, and 8.9%, respectively. Among the non-risk subgroups, the prevalence of anti-HEV was about 10 % regard- less of the result of HBsAb or HBcAb. There was no sexual and regional difference. The peak prevalence was shown at the age between 30 and 39. In the risk groups, the highest prevalence was 15% shown in the anti-HCV positive group and the lowest was the 5.9% of HBsAg positive group. There was a little higher prevalence rate in male group but there was no significant statistical difference between male and female. Anti-HEV posit,ive rates of normal group were relatively higher than the expected risk group and these results strongly suggested the possibility of existence of HEV infection in Korea, particularly in rural areas where the tap water supply facilities are not enough.

1933년에 제정한 도ㆍ부ㆍ현에 있어서의 중앙도서관의 조직 및 설비 표준의 검토

신학균,Sin, Hak-Gyun 한국도서관협회 1969 圖協月報 Vol.10 No.7