가감좌귀음(加減左歸飮)의 알레르기성 기관지천식(氣管支喘息) 반응(反應) 조절(調節) 효과(效果)

박은정,이해자,박종익,박영주,Park, Eun-Jung,Lee, Hai-Ja,Park, Jong-Ik,Park, Young-Joo 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objective : KagamJwagwiEum(KJE) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of KJE, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. Methods: HMC-1 was stimulated with phorbol 12-myristate 13-acetate(PMA) and calcium ionophore A23187. KJE by itself had no effect on viability of HMC-l. The effects of KJE on the secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$, interleukin(IL)-6 and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Results : KJE inhibited PMA plus A23187-induced $TNF-{\alpha}$ and IL-6 secretion. But KJE had no effect IL-8 secretion: KJE had immunoregulatory effects on cytokines, increased secretion of NO and $TNF-{\alpha}$ but did not effect IL-12 secretion when the cells were primed and trigged with $IFN-{\gamma}$ in the peritoneal macrophages of C57BL/6 mice. Conclusions : Taken together, these results suggest that KJE inhibit the production of inflammatory cytokines in HMC-1 cells and activate macrophages.

당귀음자(當歸飮子) 가감방(加減方)의 처방별 (A, B) 아토피성 알레르기반응 조절 효과 비교 연구

박은정,이해자,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Han, Kyeung-Hoon 대한한방소아과학회 2005 대한한방소아과학회지 Vol.19 No.2

Objective: Danguieumja-gagambang (DGEJGB), a traditional Korean prescription, has been used as therapeutics for allergic diseases such as atopic dermatitis (AD). In this study, we compared with regulatory Effect of Atopic Allergic Reaction by Prescriptions A and by Prescriptions B. Methods : To evaluate and compare the atopic allergic effectiveness of two prescription (A, B) of DGEJGB, the author investigated a possible effect of DGEJGB on mast cell-mediated allergic reaction, cytokine secretion and mRNA expression in vivo and in vitro. Results : Mast cells are a potent source of mediators that regulate the inflammatory response in allergic reaction. In mice orally administered A, B of DGEJGB ( 0.1, 0.1 and 1.0 g/kg) for 1 h, compound 48/80-induced ear swelling was significantly reduced. Significant reduced levels (P < 0.05). of tumor necrosis factor $(TNF)-{\alpha}$ was observed in the human mast cell line (HMC-1) with DGEJGB (A). IL-6 and IL-8 secretion were significantly inhibited by DGEJGB (A, B). In addition, $TNF-{\alpha}$ and IL-8 mRNA expression were reduced by DGEJGB (A) at the dose of 0.01 mg/ml without cell toxicity. Conclusions : These results suggest that DGEJGB (A) contributes to the treatment of atopic allergic reactions rather than DGEJGB (B), and that its action may be due to inhibition of cytokine secretion and mRNA expression HMC-1.

청기산(淸肌散)과 가미청기산(加味淸肌散)이 마우스의 항(抗)알레르기 및 면역반응(免疫反應)에 미치는 영향(影響)

박은정,김양귀,Park Eun-Jeong,Kim Yang-Gwi 대한한방소아과학회 1998 대한한방소아과학회지 Vol.12 No.1

Cheonggisan(CGS) is well known for its effect on such allergic disease as urticaria and atopic dermatitis. Gagamcheonggisan(GCGS) was formulated by subtracting several herbs from CGS and adding several herbs to CGS. Even though it is being used frequently in the clinicai medicine for the treatment of above hypersensitivity diseases, basic study to make sure the mechanism of its action is rare. In this study the author tried to know the effect of CGS and GCGS on the vascular permeability, contact dermatitis, granular secretion from mast cells and function of macrophages. The results obtained in this study are as follows : 1. Administration of CGS and GCGS decreased the vascular permeability induced by serotonin and histamine. The decrease by serotonin is more typical and dose-dependent. 2. Administration of CGS and GCGS inhibited foot-pad and ear swelling responses induced by sheep red blood cells and picryl chloride respectively, the inhibition of foot-pad swelling responses is bigger than that of ear swelling responses and both of them are not dependent on the dose3. Treatment of peritoneal mast cells with CGS and GCGS water extract decreased the histamine release triggered by compound 48/80 in a dose dependent fashion 4. Administration of CGS and GCGS increased the phagocvtic activity of peritoneal macrophages and treatment of peritoneal macrophages with CGS activated phagocytic function in a dose dependent fashion. 5. Administration of CGS and GCGS enhanced such reactive oxygen intermediates(ROIs) as superoxide and hydrogen peroxide production from peritoneal macrophages. 6. Treatment of CGS and GCGS activated peritoneal macrophages for the production of ROIs. The above results show that CGS and GCGS decreased the hypersensitivity reactions by inhibiting non-specific inflammatory mediator release and vascular permeability without affecting general immune responsiveness.

소아비연(小兒鼻淵)에 관한 문헌적(文獻的) 고찰(考察)

박은정,정규만,Park Eun-Jeong,Jeong Gyu-Man 대한한방소아과학회 1989 대한한방소아과학회지 Vol.3 No.1

소아중풍(小兒中風) 환자(患者)에 대한 치험(治驗) 1례(例)

박은정,이해자,나원경,장성진,안재선,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Na, Won-Kyeung,Jang, Sung-Jin,Ahn, Jai-Sun,Han, Kyeung-Hoon 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objectives : The purpose of this study is case report of stroke in childhood. The childhood cerebrovascular diseases are interesting than those seen in adulthood, because the etiology and prognosis of the disease in children are quite different from those of adults. Cerebrovascular diseases in children is more common than once recognized. However, relatively little attention has been paid to the epidemiology of childhood stroke. Methods : We experienced one case of ischemic stroke in childhood whose symptoms are similar to that of adults. There is no evidence of cerebrovascular malformation at MRI, MRA film. The cause of stroke is undetermined and just we can see the little infarction lesion on Lt. pons. We treated the patient with herb medicine, acupuncture-Tx, negative-Tx, moxa-Tx and physical treatment. Results : After the treatment of oriental medicine, the patient recovered from the stroke symptoms. Conclusion : we thought that the more follow-up is needed. So we repoert one case of cerebral infarction in children that recovered from stroke symptoms after the treatment of oriental medicine.

독사 교상후 발생한 전신적 합병증 - 혈액학적 합병증과 신경학적 합병증을 중심으로 -

박은정,윤상규,안정환,최상천,김기운,민영기,이국종,정호성,정윤석,Park, Eun-Jung,Yoon, Sang-Kyu,Ahn, Jung-Hwan,Choi, Sang-Cheon,Kim, Gi-Woon,Min, Young-Gi,Lee, Kuk-Jong,Jung, Ho-Sung,Jung, Yoon-Seok 대한임상독성학회 2009 대한임상독성학회지 Vol.7 No.2

Purpose: This study explored and evaluated the systemic complications resulting from the bite of Korean venomous snake, focussing on hematologic and neurologic features. Methods: Medical records (demographic data, clinical measurements including laboratory results, severity score, and amount of antidote administration, and hospitalization course) of consecutive patients who presented with snakebites to two university teaching hospital during a 10-year period were retrospectively reviewed. Subgroup analysis was conducted for evaluations of anti-acetylcholine esterase administration in complicated victims. Results: The 170 patients displayed occurrence rates of hematologic and neurologic complications of 12.9% and 20.6%, respectively. Among 22 patients with hematologic complications, isolated thrombocytopenia was evident in eight patients (36.4%), prothrombin time (PT) / activated partial thromboplastin time (aPTT) prolongation in 11 patients (50.0%), and both in three patients (13.6%). The mean time to recovery was $4.5{\pm}1.8$ days for isolated thrombocytopenia, and $5.1{\pm}1.8$ days for PT and aPTT prolongation. Hematologic complications could occur suddenly 1?4 days after hospitalization. Among 35 patients with neurologic complications, dizziness was evident in 16 patients (45.7%), and diplopia / blurred vision in 19 patients (54.3%). The mean time to recovery was $3.4{\pm}0.6$ days in patients receiving anti-acetylcholine esterase and $6.9{\pm}1.8$ days in those not receiving anti-acetylcholine esterase (p=0.00). Conclusion: Occurrence rates of hematologic and neurologic complications following venomous snake bite differed as compared to other studies conducted in Korea. Onset of hematologic complications can occur rapidly days after admittance. Anti-acetylcholine esterase administration may be effective in treating neurologic complications.

소아(小兒) 재발성(再發性) 삼출성(?出性) 중이염(中耳炎)에 가미형개연교탕(加味荊芥蓮翹湯)이 중이강(中耳腔) 삼출성(?出性) 내(內) Immunoglobulin-G 아형(亞型)에 미치는 영향(影響)

박은정,이윤심,Park Eun-Jeong,Lee Yun-Sim 대한한방소아과학회 2001 대한한방소아과학회지 Vol.15 No.1

The author measured IgG1, IgG2, IgG3 and IgG4 levels of 22 ears-11 ears of them had treated by antibiotics, 11 of them by Kami-hyunggyeyungyotang-of pediatric recurrent otitis media with effusion using ELISA assay, and compared them. The results were obtained as follows. 1. The level of IgG1 in Kami-hyunggyeyungyotang group was significantly higher than that in antibiotics group(P=0.002). 2. The level of IgG2 in Kami-hyunggyeyungyotang group was higher than that in antibiotics group(P=0.178). 3. The level of IgG3 in Kami-hyunggyeyungyotang group was higher than that in antibiotics group(P=0.215). 4. The level of IgG4 in Kami-hyunggyeyungyotang group was higher than that in antibiotics group(P=0.198). According to above results, Kami-hyunggyeyungyotang is considered to be used for treatment 'recurrent otitis media with effusion' by controlling the production of immunoglobulins.

학습경험을 바탕으로 학생들이 제시하는 고등학교 화학교과 내의 어려운 개념과 문지방개념 분석연구

박은정,Park, Eun Jung 대한화학회 2014 대한화학회지 Vol.58 No.1

본 연구는 과학학습의 어려움이나 낮은 흥미도의 한 원인으로 과학 교과학습에서의 중요한 통로 혹은 입구에 해당하는 문지방개념의 존재를 가정하고 특히 화학의 어떠한 개념들이 여기에 해당하는지를 알아보았다. 또한, 각 개념의 속성이 무엇이며 개념을 이해하고 "아~하"의 깨달음을 얻는 경험은 어떠했는지도 함께 알아보았다. 이를 위해, 화학 II를 학습한 239명의 고등학생이 연구에 참여하였고 설문에 대한 응답으로 화학 교과의 어려운 개념이 무엇인지, 문지방에 해당하는 개념이 무엇인지, 혹은 문지방개념을 이해한 경험이 화학 학습에 어떠한 영향을 주었는지를 설명하였다. 몰과 원자구조가 화학 교과의 문지방개념으로 제시되었고 구체적으로는 제시된 두 개념이 문지방개념이 되는 속성이 무엇인지를 집중적으로 분석하였다. 문지방을 극복하고 이해하는 것은 각자의 경험에 따르지만, 문지방개념을 분석하는 기준은 각각의 경험에 일정한 준거를 제시하여 서로 다른 경험들을 객관화 시킬뿐 아니라 개념의 과학적 의미와 본성을 잘 드러내어 준다. 특히, 교사가 제시하는 화학의 문지방개념을 조사한 사전연구와의 비교는 문지방개념의 통합적 속성이 학생들의 학습과 과학흥미도 증진에 중요함을 보여준다.

중견교수로 불릴 고달픔

박은정,Park, Eun-Jeong 한국대학교육협의회 1994 大學敎育 Vol.72 No.-

당뇨병쥐 뇌에서 Gangliosides 분석

박은정,전길자,Park, Eun-Jeong,Jhon, Gil-Ja 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6

본 연구에서는 alloxan으로 유발시킨 당뇨병 쥐의 뇌에서 gangliosides 조성 변화를 조사하였다. 뇌 조직은 alloxan 주사후 3일째 희생시켜 얻었으며, gangliosides는 Folch-Suzuki 방법을 이용하여 추출하였다. Periodate-resorcinol 법으로 sialic acids를 정량한 결과, 당뇨병 쥐 뇌에서 sialic acid 양$(375{\mu}g)$이 정상$(783{\mu}g)$에 비해 반으로 감소하였다. 당뇨병 쥐 뇌에서 GT1b, GD1b, GD1a는 감소하였고 GM3와 band 1은 증가하였다. Sialidase처리로 band 1은 sialic acid가 없는 당지질임이 확인되었다. 2D-HPTLC로 당뇨병 쥐 뇌에서 GM1a와 GD1a 사이에 존재하는 마확인된 ganglioside가 증가함이 밝혀졌다. 미지의 ganglioside를 preparative TLC로 분리하여 ${\beta}-galactosidase$를 처리한 후 HPLC로 확인한 결과 Gal-GM1, Gal-Gal-GM1임이 확인되었다. The changes of ganglioside pattern from alloxan-induced diabetic rat brain, were investigated. Gangliosides were isolated by Folch-Suzuki method and analyzed by HPTLC. Sialic acid was determined by periodate-resorcinol method. The amount of gangliosides decreased 2 folds in diabetic brain. We found decreasing GT1b, GD1b, and GD1a, increasing GM3 and band 1, and no change of GM1a. In diabetic brain band 1 was identified as simple glycolipid with no sialic acid by the treatment of sialidase. The increase of unidentified ganglioside separated between GM1a and GD1a in diabetic brain found by 2-dimensional HPTLC. Unidentified ganglioside was collected by preparative HPTLC, treated with ${\beta}-galactosidase$ and analyzed by HPLC. As the result, this ganglioside revealed Gal-GM1 and Gal-Gal-GM1.