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      • KCI등재
      • KCI등재

        $Na^+/H^+$ exchanger와 $HCO_3^-$ transporter에 의한 흰쥐 타액선 선세포내 pH 조절

        박동범,서정택,손흥규,이종갑,Park, Dong-Bum,Seo, Jeong-Taeg,Sohn, Heung-Kyu,Lee, Jong-Gap 대한소아치과학회 1998 大韓小兒齒科學會誌 Vol.25 No.2

        Intracellular pH (pHi) plays an important role in the regulation of cellular processes by influencing the acitivity of various enzymes in cells. Therefore, almost every type of mammalian cell possesses an ability to regulate its pHi. One of the most prominent mechanisms in the regulation of pHi is $Na^+/H^+$ exchanger. This exchanger has been known to be activated when cells are stimulated by the binding of agonist to the muscarinic receptors. Therefore, the aims of this study were to compare the rates of $H^+$ extrusion through $Na^+/H^+$ exchanger before and during muscarinic stimulation and to investigate the possible existence of $HCO_3^-$ transporter which is responsible for the continuous supply of $HCO_3^-$ ion to saliva. Acinar cells were isolated from the rat mandibular salivary glands and loaded with pH-sensitive fluoroprobe, 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein(BCECF), for 30min at room temperature. Cells were attached onto the coverglass in the perfusion chamber and the changes in pHi were measured on the iverted microscope using spectrofluorometer. 1. By switching the perfusate from $HCO_3^-$-free to $HCO_3^-$-buffered solution, pHi decreased by $0.39{\pm}0.02$ pH units followed by a slow increase at an initial rate of $0.04{\pm}0.007$ pH units/min. The rate of pHi increase was reduced to $0.01{\pm}0.002$ pH units/min by the simultaneous addition of 1 mM amiloride and $100{\mu}M$ DIDS. 2. An addition and removal of $NH_4^+$ caused a decrease in pHi which was followed by an increase in pHi. The increase of pHi was almost completely blocked by 1mM amiloride in $HCO_3^-$-free perfusate which implied that the pHi increase was entired dependent on the activation of $Na^+/H^+$ exchanger in $HCO_3^-$-free condition. 3. An addition of $10{\mu}M$ carbachol increased the initial rate of pHi recovery from $0.16{\pm}0.01$ pH units/min to $0.28{\pm}0.03pH$ units/min. 4. The initial rate of pHi decrease induced by 1mM amiloride was also increased by the exposure of the acinar cells to $10{\mu}M$ carbachol ($0.06{\pm}0.008pH$ unit/min) compared with that obtained before carbachol stimulation ($0.03{\pm}0.004pH$ unit/min). 5. The intracellular buffering capacity ${\beta}1$ was $14.31{\pm}1.82$ at pHi 7.2-7.4 and ${\beta}1$ increased as pHi decreased. 6. The rate of $H^+$ extrusion through $Na^+/H^+$ exchanger was greatly enhanced by the stimulation of the cells with $10{\mu}M$ carbachol and there was an alkaline shift in the activity of the exchanger. 7. An intrusion mechanism of $HCO_3^-$ was identified in rat mandibular salivary acinar cells. Taken all together, I observed 3-fold increase in $Na^+/H^+$ exchanger by the stimulation of the acinar cells with $10{\mu}M$ carbachol at pH 7.25. In addition, I have found an additional mechanism for the regulation of pHi which transported $HCO_3^-$ into the cells.

      • KCI등재
      • KCI등재

        Glass Ionomer Cement의 산부식 시간에 따른 복합레진과의 결합결에 관한 실험적 연구

        박동범,김순주 大韓小兒齒科學會 1989 大韓小兒齒科學會誌 Vol.16 No.1

        The purpose of this study was to evaluate the effects of various etching time that affect to the bonding strength between glass ionomer cement and composite resin. Two glass ionomer cements were divided into 4 groups according to 4 various etching time each other. Total 80 specimens were uniformly made and tested for thebonding strength between glass ionomer cement and composite resin by Instron. The seperation force was recorded from the composite resin to the glass ionmer cement block for each specimens. The results were as follows: 1. Etching time affects the bonding strength between glass ionomer cement and composite resin. 2. In Shofu glass ionomer cement, the 3 group, 30sec etching group was greather than any other groups and significant differences were statistically demonstrated when compared to the 1 group, non-ey-ching group.(p<0.05) 3. In GC glass ionomer cement, the 4 group, 60sec etching group, was greater than any other groups and significant differences were statistically demonstrated when compared to the 1 group, non-et-ching group.(p<0.05)

      • KCI우수등재

        ‘장기 20세기’ 농업문제의 사회적 파장과 세계헤게모니 권력순환의 식민성

        박동범(Park, Dong-beom) 비판사회학회 2017 경제와 사회 Vol.- No.116

        이 글은 ‘장기 20세기’ 농업문제의 세계적 파장을 둘러싼 오늘날의 여러 연구 상 쟁점들로부터 사회주의 정치 기획의 ‘권역주의적 창신’ 가능성을 탐색해, 이에 필요한 재이론화 작업의 요점을 잡고자 한다. 크게 세 가지다. ① 20세기 한반도-동아시아 권역일대에서 불거진 농업/먹거리 위기 관련 현안과 결부됐던 사회주의적 대항정치 기획 전반은, 역사적 식민자본주의 체계 발전의 내적 모순들 및 그 정치지리적 차등화 궤적에 새겨진 ‘변경들의 식민화’ 효과로부터 생성된 것이었다는 점, ② 역사적 사회주의(내지 서구 마르크스주의) 정치 기획 속의 ‘타자’들이 지금껏 겪고 있을 진퇴양난과 곡절의 경험을 오늘날 어떻게 새삼 재정치화, 재역사화할 것이냐는 점, ③ 일국사회주의 구상의 이론적 정당화가 다다른 정치적 난파 지점들은, 특정 집권/운동세력의 의지와 조직화된 역량 문제이기에 앞서 ‘인민주권 형식’ 자체의 문제로, 영토국가적 국민됨의 실천형식 전반이 오늘날 마주한 내적 곤경들 및 사실상의 내파 상황과 깊이 연루된 것이라는 점이다. 이로부터 필요한 건, 예컨대 ‘자본주의 4.0’ 같은 체계 내적 해법일 수 없다. 다각화된 포스트자본주의적 사회주의 정치 기획이 두루 창신되기는커녕 민주자본주의의 대증적 개혁 처방만 학술장 안팎으로 부풀려지는 저간의 상황이야말로 얼마나 불합리한지 새삼 질문해볼 필요가 있다. 사회주의적 대항민주주의 기획이 거듭 창신된 버전으로, 권역주의적인 입지에서 재생돼야 하는 이유는, 실로 가공할 다중양극화와 나란히 유기적 구성의 기술적 고도화가 자본축적의 지속 자체를 무효화하려는 오늘날 되레 무궁무진하겠다는 점에서 그렇다. This paper inquires into the potentials for a ‘regionalistic transformation’ within socialist politics perspectives, catching the concomitant point of retheorization for this inquiry. The point comprises roughly three dimensions: 1) The emergence of socialist resistant politics concerning agrarian-food crises around East-asian region throughout the 20th century generally resulted from ‘colonization of borders’ effects inscribed in immanent contradictions and geopolitical differentiation trajectories of historical colonial-capitalist development, 2) How adversities and dilemmas which has been experienced by ‘others’ in the praxis framework of historical socialist(or, Western Marxist) politics should be repoliticized and rehistoricized freshly today, 3) The wrecked points encountered by the theoretical and ideological justification for state-socialist projection are involved with the problems of the ‘people’s sovereign form’ itself much rather than those concerning the will and organized capability of some ruling forces or leading movements, having been deeply involved with impasses and de facto implosive contexts the generic territorial state-oriented practice of nationhood is today reaching at the same time. The alternative from this reflexion can not be a intra-systemic solution like ‘Capitalism 4.0’, for example. We should question what an irrational situation it is that allopathy reformist prescriptions in the existing democratic capitalism have been bloated within and beyond academic fields, far from fixing left politics toward multiple postcapitalist socialism. Because the reasons socialist practices of democracy and concomitant theorizations should be continually renewaled from now on in the horizon of regionalist perspective are today ever inexhaustible, given that really horrible global multiple polarizations coinciding with the technological acceleration of organic composition might be nullifying the durative competence in planetary accumulation of capital.

      • KCI등재후보

        세 가지 역충전재의 근단부 폐쇄 효과에 관한 전기화학적 연구

        박동,손서진,오태석,유현미,박찬제,임순호,이영규,계승 대한치과보존학회 2004 Restorative Dentistry & Endodontics Vol.29 No.4

        The purpose of this study was to evaluate the apical sealing ability of Super-EBA, MTA and Dyract-flow as retrofilling materials. Forty-eight extracted human teeth with straight and single root canal were used in this study. The root canals were prepared to a #40 apical canal size and obturated with gutter-percha. Apicoectomies were performed and root end cavities were prepared to a depth of 3mm using an ultrasonic device. The root end cavities were filled with Super-EBA, MTA or Dyract-flow. Leakage was measured using an electrochemical technique for 4 weeks. According to this study, the results were as follows. 1. Increasing leakage with time was observed in all groups. 2. No significant difference was noted among the 3 groups with time (p = 0.216). 3. No significant difference was noted among the 3 groups when measured within the same time interval (p = 0.814). The results of this study suggest that the sealing ability of Dyract-flow is equal to that of Super-EBA and MTA, and Dyract-flow may be an alternative to other materials for root-end filling. . 본 연구의 목적은 Super-EBA, MTA, Dyract-flow를 근관 역충전재로 사용하였을 때의 근단부 폐쇄 능력을 전기화학적 방법을 통해 알아보고자 하는 것이다. 48개의 단근치를 Profile을 사용하여 근관형성한 후, Gutter-percha cone으로 수직가압 충전을 시행하였다. 근단부에서 3mm 되는 부위를 bur로 절단한 후, ultrasonic device로 3mm 깊이의 역충전 와동을 형성하였다. 각 실험군은 Super-EBA, MTA, 혹은 Dyract-flow로 역충전하고 4주에 걸쳐 전기화학적인 방법으로 치근단 누출의 변화를 측정한 후, Repeated measures of ANOVA로 통계분석하였다. 그 결과 모든 시편에서 시간에 따른 치근단 누출의 증가가 관찰되었으나, 각 실험군 간에 누출의 차이는 나타나지 않았다. 이러한 결과는 Dyract-flow의 치근단 역충전재로써의 사용 가능성을 시사한다.

      • KCI등재

        An electrochemical study of the sealing ability of three retrofilling materials

        Park, Dong-Sung,Sohn, Suh-Jin,Oh, Tae-Seok,Yoo, Hyun-Mi,Park, Chan-Je,Yim, Soon-Ho,Lee, Young-Kyoo,Kye, Seung-Bum 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.4

        The purpose of this study was to evaluate the apical sealing ability of Super-EBA, MTA and Dyract-flow as retrofilling materials. Forty-eight extracted human teeth with straight and single root canal were used in this study. The root canals were prepared to a #40 apical canal size and obturated with gutter-percha. Apicoectomies were performed and root end cavities were prepared to a depth of 3㎜ using an ultrasonic device. The root end cavities were filled with Super-EBA, MTA or Dyract-flow. Leakage was measured using an elestrochemical technique for 4 weeks According to this study, the reasults were as follows 1 Increasing leakage with time was observed in all groups 2. No significant difference was noted among the 3 groups with time (p = 0.216). 3. No significant difference was noted among the 3 groups when measured within the same time interval(p = 0.814). The results of this study suggest that the sealing ability of Dyract-flow is equal to that of Super-EBA and MTA, and Dyract-flow may be an alternative to other materials for root-end filling.

      • KCI우수등재

        빙-해저지반 상호작용을 고려한 빙쇄굴 시뮬레이션 비교연구

        신문,박동,서영교 한국해양공학회 2019 韓國海洋工學會誌 Vol.33 No.6

        The ice keel gouge and seabed interaction is one of the major considerations in the design of an Arctic pipeline system. Ice keel and seabed interaction engineering models based on experimental data, which give an explicit equation for estimating the ice gouging depth, have been suggested. The suggested equations usually overestimate the ice keel gouging depth. In addition, various types of numerical analyses have been carried out to verify the suggested engineering model equations in comparison to the experimental data. However, most of numerical analysis results were also overestimated compared with the laboratory experimental data. In this study, a numerical analysis considering the contact condition and geostatic stress was carried out to predict the ice keel gouging depth and compared with the previous studies. Considering the previously mentioned conditions, more accurate results were produced compared with the laboratory experiment results and the error rate was reduced compared to previous numerical analysis studies.

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