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고로쇠 Coumarinolignans의 b-Cyclodextrin 포접화합물 제조 및 암세포증식 억제활성
임순호,정다운,윌리엄스다렌,게클러커트,김경근,신부안,이익수,김현정 한국생약학회 2015 생약학회지 Vol.46 No.2
Two coumarinolignans, cleomiscosins C (1) and D (2) were isolated from the heartwood of Acer mono, together with four compounds, 5-O-methyl-(E)-resveratrol-3-O-b-D-glucopyranoside (3), 5-O-methyl-(E)-resveratrol-3-O-b-D-apiofuranosyl-(1?6)-b-D-glucopyranoside (4), scopoletin (5), and (E)-resveratrol-3-O-b-D-glucopyranoside (6). Of them, cleomiscosins C (1) and D (2) were applied to preparing inclusion complex molecules with b-cyclodextrin (b-CD) to improve the very poor solubility in cell media. The CD complexes of 1 and 2 exhibited an enhancement of water solubility which is feasible to measure their cytotoxicity using a spectrophotometer in a cell-based assay. Anti-proliferative activity of these complex molecules was successfully estimated on HCT116 human colon cancer cells, and cleomiscosin D (2) showed anti-proliferative effects at the concentration of 1.95~31.2 mg/mL in a dose-dependent manner.
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유리단 국소의치의 Clasp설계가 지대치 운동에 미치는 영향
임순호,장익태,Yim, Soon-Ho,Chang, Ik-Tai 대한치과보철학회 1987 대한치과보철학회지 Vol.25 No.1
The purpose of this study was to clarify the effect of clasp design on abutment tooth adjacent to a distal extension base under the influence by the location of functional loading. The RPI clasp, the Akers clasp and the combination clasp were selected for evaluation. Tests were performed at 10Kg, 20Kg, 30Kg loads on the buccal, central, lingual, mesial and distal positions of loading platform of each mandibular distal extension partial denture. The laser reflexion method was used for three dimensional measurement of abutment movement, which is possible to measure precisely without contact. The movement in the mesiodistal(X), buccolingual(Y), and occlusoapical(Z) directions and the rotational movement(R) were measured, and in addition, the total movement (SV) as expressed by the three dimensional summation vector independent of direction was calculated. The data were analyzed using Student t-test, p<.05. The following results were obtained from this study; 1. Clasp design did not generally affect the direction of abutment tooth movement except the movement in an undesirable occlusal direction in case of the Akers clasp and the combination clasp. 2. The greater the load on the prosthesis, the greater was the abutment tooth movement, and the direction of abutment tooth movement was affected by positional loading. 3. Each prosthesis was dislodged from the test base under the small amount of load in the distal load position, and the buccal loading showed the greatest abutment tooth movement under the maximum load. 4. RPI clasp was evaluated as the most favorable design.
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Phenolic Constituents from the Flowers of Hamamelis japonica Sieb. et Zucc.
임순호,이영주,박기덕,이익수,신부안,정다운,Darren R. Williams,김현정 한국생약학회 2015 Natural Product Sciences Vol.21 No.3
Hamamelis japonica (Hamamelidaceae), widely known as Japanese witch hazel, is a deciduous flowering shrub that produces compact clumps of yellow or orange-red flowers with long and thin petals. As a part of our ongoing search for phenolic constituents from this plant, eleven phenolic constituents including six flavonol glycosides, a chalcone glycoside, two coumaroyl flavonol glycosides and two galloylated compounds were isolated from the flowers. Their structures were elucidated as methyl gallate (1), myricitrin (2), hyperoside (3), isoquercitrin (4), quercitrin (5), spiraeoside (6), kaempferol 4'-O-b-glucopyranoside (7), chalcononaringenin 2'-O-b-glucopyranoside (8), trans-tiliroside (9), cis-tiliroside (10), and pentagalloyl-O-b-D-glucose (11), respectively. These structures of the compounds were identified on the basis of spectroscopic studies including the on-line LC-NMR-MS and conventional NMR techniques. Particularly, directly coupled LC-NMR-MS afforded sufficient structural information rapidly to identify three flavonol glycosides (2 - 4) with the same molecular weight in an extract of Hamamelis japonica flowers without laborious fractionation and purification step. Cytotoxic effects of all the isolated phenolic compounds were evaluated on HCT116 human colon cancer cells, and pentagalloyl-O-b-D-glucose (11) was found to be significantly potent in inhibiting cancer cell growth.
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