혈관평활근세포에서 고농도 포도당이 유도한 TSP-1 발현과 MMP-2 활성에 미치는 Epicatechin의 효과

류경렬,강정훈,홍경자,정인경,장화인,이덕주,윤신희,한상준,김명석,조양혁,김명준 대한내분비학회 2006 Endocrinology and metabolism Vol.21 No.4

Background: The incidence of atherosclerosis is well correlated with the progression of type 2 diabetes mellitus. High plasma glucose in uncontrolled diabetic patients evokes many vascular complications such as atherosclerosis. Specifically, high glucose was reported to induce thrombospondin-1 (TSP-1), which activates matrix metalloproteinase-2 (MMP-2) and leads to the invasion of vascular smooth muscle cells (VSMCs) into the intima. Catechins with antioxidant effects are known to inhibit MMP-2 activity. Therefore, this study was aimed at revealing the effect of epicatechin, one of catechins, on high glucose-induced TSP-1 and the invasiveness of VSMCs.Methods: VSMCs were primarily isolated from Sprague-Dawley rat aorta. The VSMCs were incubated with different doses (30, 100 and 300 μM) of epicatechin under high glucose concentration (30 mM). The TSP-1 protein and mRNA expressions were analyzed by performing Western blotting and Northern blot analyses, respectively. RT-PCR was performed to observe the MMP-2 mRNA expression. Gelatin zymography was performed for the measurement of MMP-2 activity. Invasion assays were performed to evaluate the invasiveness of VSMCs.Results: Epicatechin inhibited the high glucose-induced TSP-1 expression and the MMP-2 activity in a dose-dependent manner. Also, epicatechin inhibited the high glucose-induced invasiveness of VSMCs across the matrix barrier in a dose-dependent fashion.Conclusion: Collectively, epicatechin may prevent the high glucose-induced proliferation and invasion of VSMCs by inhibiting the TSP-1 expression and the MMP-2 activity. Therefore, epicatechin appears to play a protective role in the development of atherosclerosis. (J Kor Soc Endocrinol 21:302~310, 2006) 연구배경: 혈당이 조절되지 않는 당뇨병 환자에서 혈장내의 고혈당은 동맥경화증과 같은 혈관계 합병증을 유발한다. 특히, 고혈당은 혈관평활근세포에서 thrombospondin-1 (TSP-1)의 분비를 촉진하며, matrix metalloproteinase-2 (MMP-2)를 활성화시켜 혈관평활근세포의 이주를 촉진한다. 한편 항산화 및 항암효과가 보고된 catechin 계열이 MMP-2 활성을 억제한다는 연구 결과가 최근 보고되었다. 따라서 본 연구는 catechin 계열에 속하는 epicatechin이 고농도의 포도당에 노출된 혈관평활근세포에서 TSP-1 발현과 이주에 미치는 영향을 조사하였다.방법: 혈관평활근세포는 Sprague-Dawley계 흰쥐 대동맥에서 분리하였다. 혈관평활근세포를 고농도의 포도당(30 mM)과 epicatechin (30, 100, 300 μM)에 노출시킨 후 TSP-1 단백질과 mRNA 발현을 Western blot과 Northern blot으로 관찰하였다. 역전사 중합효소연쇄반응을 이용하여 MMP-2 mRNA 발현을 관찰하였으며, MMP-2 활성과 혈관평활근세포의 이주능을 gelatin zymography와 Matrigel을 이용한 invasion assay로 각각 조사하였다.결과: Epicatechin은 혈관평활근세포에서 고농도의 포도당이 유도한 TSP-1 발현과 MMP-2 활성을 농도의존적으로 감소시켰다. 또한 epicatechin은 고농도의 포도당에 의해 유도된 혈관평활근세포의 이주를 유의하게 감소시켰다.

Palobutrazol과 Chlorocholine Cholride 處理가 水稻 幼苗의 生理的 特性에 미치는 影響

류경렬,허훈,김인현 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

This study was conducted to investigate the incidence of urolithiasis of Korean native cattle and beef cattle in abattoir and feedlot cattle. In addition, chemical composition of urinary calculi and field treatment of urolithiasis in feedlot cattle were also studied. The results obtained were as follows : A total of 365 heads(46.8%) of 780 cattle in abattoir were found to be urinary calculi, 55.9% of these in Korean native cattle and 44.3% in beef cattle, respectively. Seasonal incidences were relatively high in spring(57.9%) and winter(55.8%), while low in autumn(47.4%) and summer(28.0) Among 474 castrated growing cattle which feeding with pellet feeds, 318 heads(66.6%) were confirmed to urolithiasis by means of urine precipitation test. Morphological classifications of urinary calculi were appeared to 25-3% in irregular form, 24.0% in granular form, 20.7% in fine crystals, 18.0% in globular form and 12.0% in powdered form, respectively. Chemical compositions of urinary calculi were detected to relatively high levels of magnesium(17.565%) and silica(5.340%) due to feeding on Tong-il rice straw. In the field treatment of special formulated drugs to affected feedlot cattle, a total of 291 heads(91.5%) of 318 cattle were recovered as high as at the 4 weeks after treatment. The principal pathological changes were composed of hemorrhages, deceleration, degeneration and necrotic lesions on renal cortex and hemorrahages, chronic inflammatory lesions on renal pelvis and papillae, urethra and urinary bladder in the urinary system. Key words ; urolithiasis, urinary calculi, feedlot cattle.

Decreased Expression and Induced Nucleocytoplasmic Translocation of Pancreatic and Duodenal Homeobox 1 in INS-1 Cells Exposed to High Glucose and Palmitate

류경렬,유준모,이에스더,고승현,안유배,송기호 대한당뇨병학회 2011 Diabetes and Metabolism Journal Vol.35 No.1

Background: Type 2 diabetes mellitus (T2DM) is often accompanied by increased levels of circulating fatty acid. Elevations in fatty acids and glucose for prolonged periods of time have been suggested to cause progressive dysfunction or apoptosis of pancreatic beta cells in T2DM. However, the precise mechanism of this adverse effect is not well understood. Methods: INS-1 rat-derived insulin-secreting cells were exposed to 30 mM glucose and 0.25 mM palmitate for 48 hours. Results: The production of reactive oxygen species increased significantly. Pancreatic and duodenal homeobox 1 (Pdx1) expression was down-regulated, as assessed by reverse transcription-polymerase chain reaction and Western blot analyses. The promoter activities of insulin and Pdx1 were also diminished. Of note, there was nucleocytoplasmic translocation of Pdx1, which was partially prevented by treatment with an antioxidant, N-acetyl-L-cysteine. Conclusion: Our data suggest that prolonged exposure of beta cells to elevated levels of glucose and palmitate negatively affects Pdx1 expression via oxidative stress.

토탈 케어 IOT 스마트 환자 돌봄 시스템

류경렬,김현석,한현우,양윤모 한국조명·전기설비학회 2022 한국조명·전기설비학회 학술대회논문집 Vol.2022 No.11

RINm5F 세포에서 IL-1β가 유도하는 iNOS 발현과 NO 생성을 억제하는 epicatechin의 작용

류경렬,한상준,조양혁,이덕주,윤신희,김명석,김명준,민도식 대한당뇨병학회 2003 Diabetes and Metabolism Journal Vol.27 No.6

-Background: Interleukin-1β (IL-1β) stimulates the expression of inducible nitric oxide synthase(iNOS) and the production of nitric oxide(NO), lead to NO-mediated insulin, which produces cell damage. Within these signal pathways, nuclear factor-κB(NF-κB) activation is crucial, with many IL-1β-sensitive genes containing NF-κB binding sites in their promoter regions. The inhibitory effect of (-)epicatechin (EC), an antioxidant agent, on IL-1β-induced NF-κB activation, and the subsequent iNOS expression in RINm5F cells, were examined.Methods: RINm5F cells were pretreated with EC(0.8mM), and then cultured with IL-1β(10U/mL), and the iNOS mRNA and protein levels then determined by Northern and Western blots, respectively. The production of NO was measured as nitrite in the culture supernatant. The protein levels of the inhibitor of nuclear factor κB(IκB) and NF-κB DNA binding activity were determined by Western blot and electrophoretic mobility shift assay, respectively. Also, the promoter activity following transient transfection of the iNOS promoter-luciferase reporter genes into the cells were tested.Results: EC was found to significantly reduce the IL-1ß-induced NO production, and iNOS protein and mRNA levels, and also blocked the IL-1β-induced IκB protein degradation, NF-κB activation and iNOS promoter activity.Conclusion: These results suggest that EC inhibits the IL-1β-induced iNOS expression 연구배경: Interleukin-1β(IL-1β)는 inducible nitric oxide synthase(iNOS) 발현과 NO 생성을 유도하여 인슐린 분비 세포를 파괴함으로써 제1형 당뇨병 발병을 매개한다. 전사 인자 nuclear factor-κB(NF-κB)는 iNOS 유전자의 promoter에 결합하여 iNOS 발현을 활성화시킨다. 따라서 RINm5F 인슐린종양주세포에서 IL-1β가 유도한 NF-κB 활성과 iNOS 발현을 항산화 작용을 가진 (-)­epicatechin(EC)가 억제하는지를 관찰하였다. 방법: RINm5F 세포에 EC(0.8mM)를 전처리하고 IL-1β(10U/mL)를 함께 처리한 후 iNOS mRNA와 단백질 발현을 Northern blot과 Western blot으로 관찰하였고, 생성된 NO를 측정하였다. 또한 NF-κB와 결합하여 NF-κB 활성을 억제하는 IκBα 단백질과 NF-κB의 DNA 결합능을 Western blot과 젤지연분석법으로 관찰하였다. iNOS promoter-luciferase 기자 유전자를 제작하여 일시적으로 과발현시킨 후 promoter 활성도를 측정하였다.결과: EC는 IL-1β가 유도한 NO 생성과 iNOS 단백질 및 mRNA 발현을 감소시켰으며, IκBα 단백질의 분해와 NF-κB의 DNA 결합능 및 iNOS promoter 활성도를 감소시켰다.

INS-1 베타 세포에서 Exendin-4에 의한 Cyclin D1 발현에 관여하는 cAMP/PKA의 작용

류경렬,강정훈,장화인,고승현,정인경,이덕주,윤신희,한상준,조양혁,김명석,김명준 대한당뇨병학회 2005 Diabetes and Metabolism Journal Vol.29 No.4

폐결핵환자의 말초혈액에서 Activated T Cell의 변화

류경렬 ( Gyeong Ryeol Ryu ),박은숙 ( Une Sook Park ),박종화 ( Chong Hwa Park ),정판준 ( Pan Joon Jeoung ),황영실 ( Young Sil Hwang ),이왕재 ( Yang Jae Lee ),장가용 ( Ga Yong Chang ) 대한결핵 및 호흡기학회 1995 Tuberculosis and Respiratory Diseases Vol.42 No.6

원저 : 기계호흡기 관련 폐렴환자의 정량적 배양에 있어서 Endotracheal Aspirates과 Protected Specimen Brush의 비교 관찰

류경렬 ( Kyoung Ryeol Roo ),김민구 ( Min Gu Kim ),김기량 ( Gi Yang Kim ),정호경 ( Ho Gyeong Jung ),박영호 ( Young Ho Park ),강병선 ( Byeng Sun Kang ),김호철 ( Ho Chul Kim ),황영실 ( Young Sil Hwang ) 대한결핵 및 호흡기학회 1995 Tuberculosis and Respiratory Diseases Vol.42 No.5

Paclobutrazol과 Chlorocholine Chloride 처리가 수도 요묘의 생리적 특성에 미치는 영향

류경렬, 허훈, 김인현 忠北大學校 農業科學硏究所 1994 農業科學硏究 Vol.12 No.2

-본문참고-

벼 자포니카와 통일형 품종간 광전역에 따른 엽신의 광합성특성 차이

許煇,柳慶烈 韓國作物學會 1994 Korean journal of crop science Vol.39 No.4

광전역이 수도엽신의 광합성에 미치는 영향을 구명코져 일반형 2품종(추청벼, 화진벼)과 통일형 2품종(삼강벼, 칠성벼)에 대하여 분얼기에서 출수기에 이르는 기간동안 무처리, 30%, 70% 차광처리하여 분얼기와 출수기에 고저 광도 조건하에서 광합성 특성 및 이와 관련한 생리, 생태적 특성을 검토한 결과를 요약하면 다음과 같다. 1. 차광처리에 의한 건물중의 감소는 70% 차광처리구에서 많았으며, 분얼기보다 출수기가 현저하였고, LAR 및 SLA도 출수기에 높았다. 2. 분얼기 저광도(5Klux)하의 개체의 광합성 능력은 낮은 편이며, 무처리<30%<70% 차광처리의 순이었고, 고광도(33Klux)하에서는 무처리구 보다 30% 차광처리구가 가장 높았다. 3. 출수기에 단엽의 광합성 능력은 무처리에 비하여 70%차광처리구가 오히려 높았다. 4. 광합성에 대한 호흡비율(Respiration/Photosynthesisx100)은 무처리구에 비하여 차광처리구에서 전반적으로 낮았다. 5. CGR, RGR, NAR은 무처리구에 비하여 차광정도가 높을수록 감소하였다. 6. 차광처리는 수량 구성요소중 특히 1수 영화수를 감소시켜 정조수량이 감소되고, 따라서 수확지수도 저하되었다. To investigate the effect of light pretreatment on photosynthetic characteristics of leaf blade of rice, 2 varieties of japonica type and 2 varieties of tongil type were grown under 30% or 70% shading conditions from tillering stage to heading stage. Shading treatment of 70% at the heading stage produced low dry matter, and higher LAR and SLA compared with other combination of shading treatment and growing stage did. Photosynthetic activity was lower in order of 0%, 30% and 70% shading treatment under the low light intensity(5Klux) but significantly high in 30% shading treatment under the high light intensity (33Klux) at tillering stage. Photosynthetic activity under low(5Klux) and high(33Kluk) light intensity were higher in order of 70%, 30% and 0% shading treatment at heading stage. Respiration /photosynthesis ratio was lower in shading treatment than in control. CGR, RGR and NAR decreased in shading treatment. Shading treatment reduced the number of ripened grain per panicle and decreased the harvesting index.