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LPS로 유발된 대식세포의 염증반응에 대한 청상보하탕(淸上補下湯)의 효과
이경희,김홍렬,정희재,이형구,Lee, Kyung-Hee,Kim, Hong-yeoul,Jung, Hee-Jae,Lee, Hyung-Koo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1
Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.
해열탕(解熱湯)이 LPS로 자극된 대식세포에 있어 염증관련 Cytokine 발현억제에 미치는 효과
이병삼,김홍렬,김진주,정승기,이형구,정희재,Lee, Byeong-Sam,Kim, Hong-yeoul,Kim, Jin-Ju,Jung, Sung-Ki,Rhee, Hyung-Koo,Jung, Hee-Jae 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.2
Background and Objective : Haeyeol-tang, composed of Houttuyniae Herba, Lonicerae Flos, Taraxaci Herba, and Scrophulariae Radix, is widely used for alleviating the symptom of various kinds of inflammatory pulmonary disease, including asthma and COPD. We want to know whether Haeyeol-tang has an anti-inflammatory effect by analyzing expression of pro-inflammatory cytokines. Materials and Methods : We differentiated the THP-1 cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We found the safe concentration of Haeyeol-tang by using MTS assay and used PD98059 as a negative control for comparison of anti-inflammatory effect of Haeyeol-tang. Results : The RT-PCR analysis results show that the cell survival rate is over 100% within 1 ng/mL to 1 ug/mL of Haeyeol-tang and begins to decrease under 100% at 10 ug/mL. The gene expression of $IL-1{\beta}$, IL-6, IL-8, IL-10, $TNF-{\alpha}$ and $TGF-{\beta}$ levels were down-regulated when Haeyeol-tang was treated at concentrations between 1 ng/mL an 1 ug/mL on monocyte-derived macrophages. Interestingly, 1 ug/mL Haeyeol-tang-treated samples showed that the transcriptional activities of IL-8, $TNF-{\alpha}$, IL-10 and $TGF-{\beta}$ were more down-regulated than those of PD098059 $(TNF-{\alpha}$ inhibitor). At protein level, the ELISA analysis results showed that there were more remarkable (p<0.001) decreases in expression of $IL-1{\beta}$, IL-6, IL-8 and $TNF-{\alpha}$ on both the 1 ug/mL Haeyeol-tang-treated group and the PD98059-treated group than the LPS-treated group. Conclusion : We conclude that Haeyeol-tang has an anti-inflammatory effect by inhibiting expression of pro-inflammatory cytokines and chemokines at mRNA and protein levels. These results may provide us a promising way to care for general inflammatory diseases as well as inflammatory pulmonary disease, including asthma and COPD, with further clinical study.
제천전(濟川煎)과 약물(藥物) 가감(加減)이 흰쥐의 사하작용(瀉下作用)에 미치는 영향(影響)
이승희,이상준,박수연,김홍렬,박성규,Lee Seung-Hee,Lee Sang-Jun,Park Soo-Yeon,Kim Hong-Yeoul,Park Seong-Kyu 대한한의학방제학회 2002 大韓韓醫學方劑學會誌 Vol.10 No.1
We have examined the purgative effect of three Jechun-jun formulas in sprague dawley(SD) rat. Three jechun-jun formulas were Jechun-jun(Sample I ) and augmented Jechun-jun(Sample II) and augmented Jechun-jun add rhubarb(sample III ). We examined the amount and the humidity of feces in rat. The experimental animals were devided into four groups. as control group and three Jechun-jun (sample I, II, III). We administerd the water extract of sample I, II, III to rat per oral for 8days long. After every 24hours measured the amount of wet feces from rats in metabolic cage. Humidity rate of feces from rat was at first measure wet feces for 24hours (W) and measure dried feces (W1) and then we consider W-W1 as humidity. High humidity rate means constipation changes into moistening stool. The amount of wet feces and humidity rate of feces in rats were increased in sample I, II, III. Sample I has highest humidity rate of feces. so showed strong moistening effect. Sample II has mild effect in treating constipation. sample III has most amount of wet feces. in conclusion Jechun-jun has an effect of moistening stool. and augmented Jechun-jun add rhubarb has strong purgative effect.
백과엽(白果葉) 및 자오가(刺五加) 가미사물탕(加味四物湯)이 백서(白鼠)의 흉부대동맥(胸部大動脈) 혈관이완(血管弛緩)에 미치는 영향
김형환,박수연,강순아,김홍렬,안덕균,박성규,Kim Hyung-Hwan,Park Soo-Yeon,Kang Soon-Ah,Kim Hong-Yeoul,Ahn Duk-Kyun,Park Seong-Kyu 대한한의학방제학회 2003 大韓韓醫學方劑學會誌 Vol.11 No.2
We have examined the relaxational response to the water extract of Angelica gigas $N_{AKAI}$ (AG), Gingko biloba $L_{INNE}$ (GB), Acanthopanax senticosus $H_{ARMS}.$ (AP) and Augumented-Four-Substance Decoction (AG-FSD, GB-FSD, AP-FSD) in isolated thoracic aorta from sprague dawley(SD) rat. Rat thoracic aorta was investigated in vessel segments suspended for isometric tension recording by polygraph. Responses to AG, GB, AP and AG-FSD, GB-FSD, AP-FSD were investigated in vessels precontracted with 5-hydroxytryptamine(5-HT) were compared in vasodilation effect. We found that the thoracic aorta segments responded to AG, GB, AP and AG-FSD, GB-FSD, AP-FSD with a dose-dependent vasodilation. The 5-hydroxytryptamine induced contraction at $10^{-4}M$ were inhibited by 26.3%, 75.8%, 87.5% and 6.9%, 22.6%, 30.8% after addition of the 0.1 g/mL water extract of AG, GB, AP and AG-FSD, GB-FSD, AP-FSD. In conclusion, AG, GB, AP and AG-FSD, GB-FSD, AP-FSD induced relaxation in the isolated rat thoracic aorta were composed of dose-dependent relaxation. AP-FSD has very potent vasodilation.
무주지역에서 채취한 산삼 생육 토양미생물에 대한 동정 연구
신동엽,박용일,김홍렬,안덕균,박성규 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2
objectives : Microorganisms were isolated from soils remaining attached to the roots of Wild Panax ginseng C.A. Mey. naturally grown in Moo-Ju area. methods : As a preliminary study for the establishment of wild ginseng radix discrimination system based on area of growth, these isolates were identified by using mainly API 20NE and API 50CHB kits and the microbial consortia were compared. From the sample of Moo-Ju area, 9 and 6 strains were isolated and identified after the incubation of agar plates at 30℃ and 37℃, respectively. results : The results showed that 2 strains of soil bacteria, Aeromonas hydrophilia, and Bacillus cereus commonly existed in the roots of wild ginseng of both 30℃ and 37℃. And that 6 strains, Pseudomonas putida, Stenotrophomonas maltophilia, Pseudomonas fluorescence at 30℃ and Bacillus subtilis, Empedobacter brevis, Brevi. laterosporus at 37℃, inhabited specifically in the roots of wild ginseng of Moo-Ju area. conclusion : These results suggest that comparison of microbial consortia associated with roots of wild ginseng could be used for the establishment of a naturally growing wild ginseng radix discrimination system.