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Saccharomyces cerevisias 에서 발현시킨 인성장호르몬의 정제 및 특성 확인
원특연,제훈성,김천형,최형배,한규범,박순재 ( Teug Yeon Won,Hoon Sung Jeh,Chun Hyung Kim,Hyung Bae Choi,Kyu Beom Han,Soon Jae Park ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
The recombinant human growth hormone (rHGH) was expressed in Saccharomyces cerevisiae. The aggregated rHGH molecules were solubilized by rasing pH of the cell lysates. When pH of the solution was lowered to around 6.5, substantial amount of the contaminating yeast proteins was effectively removed. The rHGH was further purified by successive chromatographic steps including DEAE cellulose, Sephacryl S-200, DE-52, and Phenyl-sepharose. The specific activity of the purified rHGH was 2.7IU per mg, when assessed by radioreceptor assay. This value was higher than that of pituitary-derived international standard HGH, indicating that rHGH was correctly folded.
Hoe-Yune Jung,An-Na Lee,Tae-Jun Song,Hyo-Sun An,Young-Hoon Kim,Kyu-Dae Kim,In-Bo Kim,김경심,한백수,김천형,김광수,김종배 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.7
The beneficial effects of exercise on overall health make it desirable to identify the orally active agents that enhance the effects of exercise in an effort to cure metabolic diseases. Natural compounds such as resveratrol (RSV) are known to increase endurance by potentiating mitochondrial function. Korean mistletoe (Viscum album coloratum) extract (KME) has characteristics similar to those of RSV. In the present study, we determined whether KME could increase mitochondrial activity and exert an anti-fatigue effect. We found that KME treatment significantly increased the mitochondrial oxygen consumption rate (OCR) in L6 cells and increased the expression of peroxisome proliferator-activated receptor c coactivator (PGC)-1a and silent mating type information regulation 2 homolog 1 (SIRT1), two major regulators of mitochondria function, in C2C12 cells. In the treadmill test, KME-treated mice could run 2.5-times longer than chow-fed control mice. Additionally, plasma lactate levels of exhausted mice were significantly lower in the KME-treated group. In addition, the swimming time to exhaustion of mice treated with KME was prolonged by as much as 212% in the forced-swim test. Liver and kidney histology was similar between the KME-treated and phosphate-buffered saline-treated animals, indicating that KME was nontoxic. Taken together, our data show that KME induces mitochondrial activity, possibly by activating PGC-1a and SIRT1, and improves the endurance of mice, strongly suggesting that KME has great potential as a novel mitochondria-activating agent.