심바스타틴이 인체망막색소상피세포에서 생산된 혈관내피성장인자에 미치는 효과

김경진,김경실,김나래,진희승,Kyoung Jin Kim,Kyong Sil Kim,Na Rae Kim,Hee Seung Chin 대한안과학회 2012 대한안과학회지 Vol.53 No.6

Purpose: To examine the effect of simvastatin on vascular endothelial growth factor (VEGF) expression in cultured human retinal pigment epithelial (RPE) cells under oxidative stress. Methods: RPE cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 hours of incubation with various concentrations of simvastatin or H2O2. Cultured human RPE cells were pretreated with various concentrations of simvastatin and then incubated with 100 μm H2O2. After 24 hours of incubation, an enzyme-linked immunosorbent assay (ELISA) was performed to evaluate the expression of VEGF. Results: Simvastatin showed no toxicity up to 10 μm, but cell viability gradually decreased with increased concentration of simvastatin. Human RPE cells showed increased VEGF expression when exposed only to H2O2. When RPE cells were preincubated with simvastatin and later exposed to H2O2, VEGF expression was relatively lower. Conclusions: Simvastatin downregulated the expression of VEGF in human RPE cells under oxidative stress. Simvastatin may have some clinical benefits in preventing retinal diseases associated with VEGF. J Korean Ophthalmol Soc 2012;53(6):849-855

좌심실형성부전증후군 환아의 치과치료를 위한 전신마취 시 강화 후두마스크를 이용한 기도 유지

김경진,신터전,현홍근,김영재,김정욱,장기택,이상훈,김종철,서광석,Kim, Kyung Jin,Shin, Teo-Jeon,Hyun, Hong-Keun,Kim, Young-Jae,Kim, Jung-Wook,Jang, Ki-Taeg,Lee, Sang-Hoon,Kim, Chong-Chul,Seo, Kwang-Suk 대한치과마취과학회 2013 Journal of Dental Anesthesia and Pain Medicine Vol.13 No.4

Despite the laryngeal mask airway (LMA) has been widely used in anesthesia, its use is rare in the field of dentistry. Placing LMA in the oral cavity may interrupt dental treatment. However, there are some circumstances in which LMA is more advantageous than tracheal intubation for managing the airway. Especially, the reinforced LMA has a flexible tube shaft, rendering it more accessible for dental treatment. We report a case of dental treatment of a 3-year-old patient with hypoplastic left heart syndrome combined with pulmonary hypertension using reinforced LMA for airway management under general anesthesia. We also discuss the considerations of utilizing the reinforced LMA for dental treatment.

HPLC-ICP/MS 와 ICP/MS를 이용한 FAPAS 07187 쌀의 총비소, 무기비소, 카드뮴 측정

노현명 ( Hyun Myung Noh ),김지영 ( Ji Young Kim ),김원일 ( Won Il Kim ),( Anitha Kunhikrishnan ),김민지 ( Min Ji Kim12 ),김경진 ( Gyeong Jin Kim ),박기문 ( Ki Moon Park ),류지혁 ( Ji Hyock Yoo ),김두호 ( Doo Ho Kim ),이영자 ( Y 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

Metal (loid)s such as environmental contaminants affect food safety. Metal (loid)s exist trace extremely in agriculture products. Therefore it is important to analyze accurately about trace these metals. High resolution techniques and quality controls are needed to analyze these materials. In order to achieve quality results, standardized methods and techniques are required. So ISO(International Organization for Standardization) of laboratory proficiency testing is determination of laboratory testing performance by means of inter-laboratory comparison. The values have been derived as a consensus of a number of laboratories using a variety of methods. Proficiency tests were applied to analyze the metal(loid)s [inorganic arsenic (As), total As and cadmium (Cd)] in powdered rice FAPAS 07187 (Food Analysis Performance Assessment Scheme; February-April 2013). The rice powder sample was extracted with 1% HNO3 for inorganic As for microwave-assisted extraction using water at 95℃ for 90 min and their analysis by HPLC/ICPMS. Also, HNO3 for total As and Cd using microwave-assisted extraction using at 200℃ for 50 mins and analysis by ICP/MS. Validation check was performed for accuracy using CRM (NMIJ CRM 7503-a) for inorganic As, and CRM (IRMM-Rice CRM) for total arsenic and cadmium. The result were satisfactory. FAPAS 07187 the z-scores of inorganic As, total As and Cd in powdered rice were-1.8,-0.9 and 0.2(lzl<2, respectively).

환절기 농장 점검사항 - 환절기 약품 및 소독제관리 요령

김경진,Kim, Gyeong-Jin 대한양계협회 2016 월간 양계 Vol.48 No.9

여름철 농장 점검사항 - 여름철 고온 스트레스 예방법

김경진,Kim, Gyeong-Jin 대한양계협회 2016 월간 양계 Vol.48 No.6

In Vitro Construction and Characterization of the Bacteriophage P4 Derivative, P4 sid71 cosP2, Containing the Bacteriophage P2 cos Region

김경진,Kim, Kyoung-Jin The Microbiological Society of Korea 2013 미생물학회지 Vol.49 No.1

박테리오파지 P2 sir 변이체는 그것의 위성파지인 박테리오 파지 P4를 위해 비효율적인 도움파지로 알려졌다. 이러한 현상을 "P2 sir-관련 도움파지 비효율성"이라고 부르고 있으며, P2와 P4의 cos 지역에서의 DNA 염기배열 순서 차이가 이러한 현상을 나타나게 한다고 생각되었다. 이를 검증하기 위해, 여러 단계의 in vitro DNA 조작을 거쳐 P2의 cos 지역을 함유하는 유도체 P4인 P4 sid71 cosP2를 조성하였다. 일단계 생장 실험을 통해 그것의 후손 방출량을 결정하였다. 그 결과는, P4 sid71 cosP2에서 P4의 cos 지역을 P2의 cos 지역으로 대체한 것이 "P2 sir-관련 도움파지 비효율성"을 극복한 것으로 나타났다. P2 야생형과 P2 sir 돌연변이형을 도움파지로 삼아 준비한 P4 sid71 cosP2 파지 농축액들을 CsCl 부양 균등밀도 편차실험으로 분석하였다. 그 결과, 양 경우 모두 3개의 P4 유전체를 가진 파지 입자가 우세한 것으로 나타났다. Bacteriophage P2 sir mutants are inefficient helpers for their satellite bacteriophage P4. The term, "P2 sir-associated helper inefficiency" has been used to define this phenomenon and it has been suggested that the DNA sequence difference between the cos region of P2 and that of P4 is responsible. To test this hypothesis, P4 derivative phage, P4 sid71 cosP2, containing the cos region of P2 and sid71 allele was constructed through several in vitro DNA manipulation steps. Its burst size was determined using a one-step growth experiment. The results showed that the substitution of the cos region of P2 for the cos region of P4 in P4 sid71 cosP2 overcame "P2 sir-associated helper inefficiency". P4 sid71 cosP2 stock phages prepared with P2 wild type helper and P2 sir helper were analyzed using a CsCl buoyant equilibrium density gradient experiment. The results revealed that the phage particles containing three copies of the P4 genome were the predominant particles in both cases.

이산대수 기반 Diffie-Hellman형 표준 키 분배 프로토콜의 안전성 분석에 관한 연구

김경진,김성덕,심경아,원동호,Kim, Kyung-Jin,Kim, Sung-Duk,Shim, Kyung-Ah,Won, Dong-Ho 한국정보처리학회 2002 정보처리학회논문지 C : 정보통신,정보보안 Vol.9 No.6

인터넷과 같은 첨단의 정보 전송 시스템이 발달함에 따라 네트워크 상에서 전송되는 메시지에 대한 기밀성을 제공하기 위해서 암호 시스템의 사용이 증가하고 있으며 그 중요성은 더욱 강조되고 있다. 안전한 암호 시스템을 구현하는 데 있어서 키 분배 프로토콜은 가장 필수적인 요소이며, 지금까지 여러 키 분배 프로토콜들이 표준으로 제안되었으나 이에 대한 엄밀한 안전성 증명은 아직까지 부족한 실정이다. 따라서 본 논문에서는 ANSI X9.42의 Diffie-Hellman형 표준 키 분배 프로토콜의 특징을 자세히 분석하고 이를 기반으로 여러 능동적 공격자 모델에 대한 프로토콜의 안전성을 증명하고자 한다 According to the wide-spread of information transmission system over network, the use of cryptographic system to provide the integrity of transmitted message over network is increasing and the importance of that is emphasized. Because the security of the cryptographic system totally relies on the key, key management is a essential part of cryptographic system. A number of key agreement protocols have been proposed to far, but their rigorous security analysis is still open. In this paper, we analyze the features of Diffie-Hellman based standard key agreement protocols and provide the security analysis of those protocols against several kinds of active attacks.

Hugo $H\"{a}ring$의 '신건축(Neues Bauen)' 사상과 그 이론 발전의 상징론적 측면에 대한 고찰

김경진,임석재,Kim, Kyoung-Jin,Yim, Seock-Jae 한국건축역사학회 2003 건축역사연구 Vol.12 No.2

Hugo $H\"{a}ring$(1882-1952) belonged to that special generation of architects born in the 1880s which became responsible for the establishment of Modern Movement in the 1920s as W. Gropius, Mies van der Rohe and Le Corbusier, etc. Although he have been overlooked by many historian, He was a key figure of the Modern architecture and as the main theorist for Organic stream in German architecture. He is well-known for his theory of 'Neues Bauen(New Building)', the organic functionalism that is epitomized as the design process from the inside outwards, starting with the life-processes of dwelling. So he argued that the builder must become aware of the life process his building is to serve, and he should not impose a form but try to find the form. These concepts are expressed well in his key-words, the 'Organwerk(organ-work)' and 'Leistungsform(form as achievement)'s. $H\"{a}ring's$ theory can be found in the short early essay, 'Wege zur Form(approaches to form)' of 1925. But His concept of 'function' is based on the speciality and individual identity that concerned him from the start, not purely pragmatic aspects. After 1940s his theory moved increasingly in this direction. He defined this as the transition from 'Organwerk' to 'Gesetaltwerk', from mere anatomy to essence, being, personality, life. It suggest that Hugo Haring's idea of Gestalt is a dimension of mystical or symbolic meaning. This paper Is about the way in which this theoretical transition can be parallel with contemporary philosophers as E. Cassirer's philosophy of symbolic forms and M. Heldegger's phenomenology. And the key example of this viewpoint is <Gut Garkau>(1921-1926) near Lubuk in Germany, with its 'cowshed' of pear shaped plan devised around the requirements and rituals of farm. This study presents the symbolic conception of Hugo $H\"{a}ring's$ theory can propose the ability of a symbolic intuition as a view that re-integrate technical thinking with knowledge of other kinds beyond the immediate material.

Construction and characterization of the bacteriophage P4 derivatives whose genome size suitable for packaging into a P2_sir3-sized head

김경진,Kim, Kyoung-Jin The Microbiological Society of Korea 2015 미생물학회지 Vol.51 No.1

"P2 sir-관련 도움파지 비효율성"이라는 용어는 P2 sir 변이체가 그들의 위성 박테리오파지인 P4에 대해 도움파지 역할을 충분히 못하는 것을 가리키는 용어이다. 이 연구의 목적은 이러한 P2 sir-관련 도움파지 비효율성을 극복하는 요인들을 조사하는 것이다. 우선 P2의 cos 영역을 함유하는 P4 sid71 cosP2가 P2 sir3의 도움파지 비효율성을 극복하는지를 조사하였다. 그 결과 P4 sid71 cosP2는 P2 sir3의 도움파지 비효율성을 극복하지 못하였다. P2의 cos 영역 대신에 $P2_{sir}$-sized head에 packaging되는 DNA 크기가 도움파지 비효율성을 극복하는 중요한 요인으로 나타났다. 이 연구에서는, DNA 조작을 통해 packaging 되는 DNA 크기가 P4 ost1과 P4 ost2 사이가 되는 세 종류의 P4 유도체를 조성하였다. 그 중 하나인 P4 sid71 delRI::apr의 CsCl 균등밀도차실험을 거쳐 packaging되는 DNA의 크기를 확인하였다. P4 유도체들의 후손방출량 실험에 따르면 그들은 모두 P2sir3-관련 도움파지 비효율성을 극복하였다. $P2_{sir3}$-sized head에 잘 packaging되는 P4 유도체의 크기는 28-29 kb로 나타났다. The term "P2 sir-associated helper inefficiency" has been used to define the inefficient helper capability of P2 sir mutants for their satellite bacteriophage P4. The aim of this study was to investigate the factors overcoming P2 sir-associated helper inefficiency. At first, we verified whether the P2 cos region containing P4 sid71 cosP2 could overcome P2 sir-associated helper inefficiency with P2 sir3. The result was that P4 sid71 cosP2 could not overcome P2 sir-associated helper inefficiency with P2 sir3. Instead of cos region of P2, the size of the DNA packaged into a $P2_{sir}$-sized head seems to be important for overcoming P2 sir-associated helper inefficiency. In the present work, three kinds of P4 derivatives with packaged DNA sizes between those of P4 ost1 and P4 ost2, were constructed through DNA manipulation. In one P4 derivative, P4 sid71 delRI::apr, the size of the packaged DNA was identified with a CsCl buoyant equilibrium density gradient experiment. According to the burst sizes of the P4 derivatives, they could overcome P2 sir3-associated helper inefficiency. The size of the P4 derivative DNA suitable for packaging into a $P2_{sir3}$-sized head was 28-29 kb.

11월의 양돈관리 포인트

김경진,Kim, Gyeong-Jin 대한양돈협회 2007 養豚 Vol.29 No.11