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아스콜빈산과 Aflatoxin B_1 반응생성물의 돌연변이 유발성
권미향,박건영,최홍식,백형석 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.5
아스콜빈산(Ascorbic acid, AA)과 Aflatoxin B_1(AFB_1)의 반응생성물(37℃, pH5에서 5일간 반응) 그리고 산화제(cupric ion, Cu^2+)와 환원제(cysteine) 존재하에서 얻어진 동 반응생성물의 돌연변이 유발성에 대한 특성을 Salmonella assay system에 의하여 살펴보았다. AA와 AFB_1의 반응생성물의 돌연변이 유발성은 대조군인 AFB_1의 경우보다 73% 감소하였고, Cu^2+이 첨가된 AA와 AFB_1 반응생성물의 돌연변이 유발성은 더욱 감소되어 거의 돌연변이성을 나타내지 않았다. 그리고 cysteine의 존재하의 AA와 AFB_1 반응생성물의 돌연변이 유발성은 AFB_1 단독보다는 현저히 낮아졌으나, AA+AFB_1 반응생성물보다는 약간 낮고, Cu^2+AA+AFB_1 반응생성물보다는 약간 높은 경향을 보였다. 그러므로 AA와 AFB_1의 반응 체계에 있어서 AA는 AFB_1의 분해 또는 전환에 영향을 주고 있으며 동 반응생성물은 AFB_1 단독이 갖는 돌연변이 유발성보다는 현저히 낮았고 이 때 산화환원제의 존재 역시 돌연변이성에 영향을 주었다. Aflatoxin B_1(AFB_1) was reacted with ascorbic acid(AA) alone, with AA plue cysteine and with AA plus cupric ion for 5 days(at 37℃ and pH 5), and the mutagenicity of the reaction products was tested with Salmonella typhimurium TA 100. About 70% of AFB_1 induced mutagenesis was reduced when AFB_1 reacted with AA. This decreasing effect was more severe when AFB_1 reacted with AA plus cysteine. The mutagenicity of AFB_1 when reacted with AA plus cupric ion was almost completely inhibited, however, cupric ion itself was shown to enhance the mutagenicity of AFB_1. Therefore, AFB_1 may be degraded in the presence of AA under the given reaction condition and the reaction products was observed to have nonmutagenic effects on the bacterial mutagenecity trials.
권미향,임은정,성하진 ( Mee Hyang Kweon,En Jung Lim,Ha Chin Sung ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1
During the screening for anti-complementary activity from 10 kinds of edible mushrooms, an alkali extract of Agaricus bisporus showed the highest activity through the complement fixation test. The crude anti-complementary material(AB-O) from Agaricus bisporus was obtained by the alkali extraction using 1 N NaOH containing 5% urea(65℃), followed by methanol reflux, dialysis and lyophilization. The fraction AB-O showed potent anit-complementary and anti-tumor activity against sarcoma-180 injected mice. The fraction AB-O was divided into 5 fractions(AB-20, AB-40, AB-60, AB-80, AB-A) by gradual acetone precipitation. Among them fraction AB-20 having the highest activity and yield was found to contain 39% carbohydrate and 46% protein. The anti-complementary protein-bound polysaccharide AB-20 consisted of glucose, arabinose, xylose, galactose and mannose in a molar ratio of 6.49 : 1.98 : 1.24 : 1.00 : 0.71, respectively and its main component amino acids were alanine(20.59%), isoleucine(16.85%), glutamine+glutamic acid(14.12%) and leucine (13.83%). The anti-complementary activity of AB-20 was decreased greatly by periodate oxidation, but decreased slightly by pronase digestion. This indicates that polysaccharide moiety is corelated with the anti-complementary activity and that protein is also involved in the activity.
아스콜빈산과 Afatoxin $B_1$ 반응생성물의 돌연변이 유발성
권미향,박건양,최홍식,백형석 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.5
아스콜빈산 (Ascorbic acid, AA)과 Aflatoxin ($B_1\; AFB_1$) 의 반응생성물 ($37^{\circ}C$ pH5에서 5일간 반응) 그리고 산화제(cupric ion, $Cu^{2+}$)와 환원제(cysteine) 존재하에서 얻어진 동 반응생성물의 돌연변이 유발성에 대한 특성을 Salmonella assay system에 의하여 살펴보았다. AA와 $AFB_1$의 반응생성물의 돌연변이 유발성은 대조군인 $AFB_1$의 경우보다 73 감소하였고, $Cu^{2+}$이 첨가된 AA와$AFB_1$반응생성물의 돌연변이 유발성은 더욱 감소되어 거의 돌연변이성을 나타내지 않았다. 그리고 cysteine의 존재하의 AA와 $AFB_1$ 반응생성의 돌연변이 유발성은 $AFB_1$ 단독보다는 약간 낮고, $Cu^{2+}$나 AA+$AFB_1$ 반응생성물보다는 약간 높은 경향을 보였다. 그러므로 AA와 $AFB_1$의 반응 체계에 있어서 AA는 $AFB_1$의 분해 또는 전환에 영향을 주고 있으며 동 반응생성물은 $AFB_1$ 단독이 갖는 돌연변이 유발성보다는 현저히 낮았고 이 때 산화환원제의 존재 역시 돌연변이성에 영향을 주었다. Aflatoxin $B_1$ ($AFB_1$) was reacted with ascorbic acid (AA) alone, with AA plue cysteine and with AA plus cupric ion for 5 days (at $37^{\circ}C$ and pH 5), and the mutagenicity of the reaction products was tested with Salmonella typhimurium TA 100. About 10% of AFBl induced mutagenesis was reduced when $AFB_1$ reacted with AA. This decreasing effect was more severe when $AFB_1$ reacted with AA plus cysteine. The mutagenicity of $AFB_1$ when reacted with AA plus cupric ion was almost completely inhibited, however, eupric ion itself was shown to enhance the mutagenicity of $AFB_1$. Therefore, $AFB_1$ may be degraded in the presence of AA under the given reaction condition and the reaction products was observed to have nonmutagenic effects on the bacterial mutagenecity trials.
권미향(Mee Hyang Kweon),박미경(Mee Kyung Park),나경수(Kyung Soo Ra),성하진(Ha Chin Sung),양한철(Han Chul Yang) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2
Screening of anticoagulant activity was conducted for the hot water extracts of 73 kinds of medicinal herbs, 41 kinds of Korean edible plants. and 5 kinds of sea weeds using plasma recalcification test(Tr). In the first screening several extracts of the plants, Alisma calnaliculation, Corydalis ternate. Panax notoginseng. Allium sativum, Ganoderma lucidum, Codium fragile, showed high activities. When the plants were reextracted with various solvent conditions, acidic water extracts of Codium fragile showed the highest activity in APTT. A crude polysaccharide fraction(CF-1) was prepared by methanol reflux, ethanol precipitation, dialysis and lyophilization of the acid extracts. CF-1 comprised 80.8% total sugar consisting of arabinose, galactose and glucose as the main monomers, 8.7% protein, and 13.3% sulfate. The anticoagulant activity of CF-1 was not changed by pronase digestion, but decreased by periodate oxidation, and this indicated that the anticoagulant activity was attributed to the polysaccharide portion.
김신덕,권미향,김창진,유익동 ( Shin Duk Kim,Mi Hyang Kweon,Chang Jin Kim,Ick Dong Yoo ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1
A plant growth inhibitor has been isolated from culture filtrate of strain 80-8, a soil isolate. This compound was identified as hygromycin, an antibiotic against gram-positive and gram-negative bacteria. This is the first report that hygromycin inhibits the growth of plant.
고사리 열수 추출물로부터 보체계 활성화 산성 다당의 분리 및 특성
오병미,권미향,나경수 한국식품영양학회 1994 韓國食品營養學會誌 Vol.7 No.3
고사리 열수 추출물로부터 보체계 활성화 산성 다당을 분리하였다. 열수 추출물의 메탄올 환류, 에탄올 침전, 투석 및 동결건조를 통하여 조다당획분인 HPA-1을 분리하였다. HPA-1은 총당 81.80%, 산성당 30.40%, 단백질 15.60%를 함유하였으며, 주요 구성당으로서 rhamnose, galactose, galacturonic acid, glucuronic acid등이 포함되어 있었다. HPA-1을 cetavlon(cethyltrimethyl ammonium bromide)처리에 의해 HPA-2, 3, 4, 5의 획분들로 분리하였으며, 활성과 수율이 가장 높았던 HPA-2로부터 DEAE-Sepharose CL-6B 이온교환 및 Sephadex G-100 and Sepharose CL-6B, Sepharose CL-4B 겔여과 크로마토그라피를 통하여 두 개의 주요 활성 다당체인 HPA-2-Ⅳa와 HPA-2-Ⅴa-2를 정제하였다. 이 다당들은 HPLC에서 단일 획분으로 확인되었으며 분자량은 각각 500,000과 560,000으로 측정되었다. HPA-2-Ⅳa는 총당 81.63%, 산성당 31.61%로 구성되었으며 fucose, galacturonic acid, glucuronic acid를 1.40:0.97:1.88의 몰비율로 함유하였다. HPA-2-Ⅴa-2는 주요 구성당으로서 rhamnose, galactose, galacturonic acid를 1.00: 1.38: 139의 몰비율로 함유하였다. 이 다당들의 Ca^2+존재 유무시의 황보체 활성 측정과 교차면역 전기영동 결과, 고전경로(classical pathway)와 부경로(alternative pathway)를 통한 보체계 활성화함에 관여함을 알 수 있었다. From the hot water extract of bracken (Pteridium aquilinum var. latiusculum), a Korean wild edible plant, anti-complementary acidic polysaccharldes were isolated. Crude polysaccharide fraction(HPA-1) was obtained by methanol reflux, ethanol precipitation, dialysis, and lyophilization. HPA-1 contained 81.80% of total sugar, 30.40% of uronic acid, and 15.60% of protein. HPA-1 was purified consecutively by cetavlon fractionation and chromatography including ion exchange with DEAE-Sepharose CL-6B and gel permeation with Sephadex G-100 and Sepharose CL-6B. HPA-2-Ⅳa and HPA-2-Ⅴa-2 were nearly homogeneous on HPLC and had 500,000 and 560,000 daltons of molecular weights, respectively. HPA-2-Ⅳa consisted of fucose, galacturonic acid, and glucuronic acid at the molar ratio of 1.40:0.97:1.88. HPA-2-Ⅴa-2 was composed of rhamnose, galactose, and galacturonic acid at the molar ratio of 1.00 : 1.38 : 1.39. The polysaccharides were found to activate the C3 component both in the presence and in the absence of Ca^2+ through the crossed-immunoelectrophoresis suggesting that those involved in both classical and alternative complement pathway.