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대장균에서 무작위 샤인-달가노 서열이 소성장호르몬 유전자 발현에 미치는 영향
나경수,나경수,백형석,이용세 한국생명과학회 2000 생명과학회지 Vol.10 No.4
In order to search for the effects of Shine-Dalgarno (SD) sequence and nucleotide sequence of spacer region (SD-ATG) on bGH expression, oligonucleotides containing random SD sequences and a spacer region were chemically synthesized. The distance between SD region and initiation codon (ATG) was fixed to 9 nucleotides in length. The expression vectors have been constructed using pT7-1 vector containing a T7 promoter. Positive clones were screened with colony hybridization and named pT7A or pT7B plasmid series. The selected clones were confirmed by DNA sequencing and finally, 19 clones having various SD combinations were obtained. When bovine growth hormone was induced by IPTG in E. coli BL21(DE3), all cells harboring these plasmids produced a detectable level of bGH in western blot analysis. However, various SD sequences did not affect on bGH expression, indicating that the sequences of SD and the spacer region did not sufficiently destabilize mRNA secondary structure of bGH gene. Therefore, these results indicate that the disruption of mRNA secondary structure might be a major factor for regulating bGH expression in the translational initiation process.
아담신화와 무위자연론의 비교 : 문화의 개념 정립을 위한 시고
羅景洙 全南大學校 人文科學硏究所 1993 용봉인문논총 Vol.22 No.-
A common feature that we can find out from comparing the Myth of Adam with the Doctrine of Inactive Nature is that both have a same idea about Knowledge Both the Garden of Eden and the World of Inactive Nature are places where Knowledge connot maintain its position. On the contrary, the Lost Paradise and the earthly world are places where we seek after Knowledge, and are supported by Knowledge. Lao-tzu said that the best goodness is letting matters their natural courses. That idea is comparable to the idea of Myth of Adam: 'We can live permanently only by not eating the fruit of the Tree of Knowledge' The Lost Paradies is a cultural life or cultural place in the Myth of Adam, because it is the place where we must work and produce something useful. That is to say, the fruit of the Tree of Life is a symbol of permanent life, and the fruit of the Tree of Knowledge is a symbol of Knowledge. So we can conclude that the places, where we can be workless and unproductive, is the world of Inactive Nature, while the place where we must work and produce something is artificial and cultural world.