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Rhizobium Japonicum 1 - 23 Glutamine Synthetase Ⅰ의 분리 정제 및 성질
성하진,이왕식,방원기 생화학분자생물학회 1993 BMB Reports Vol.22 No.4
Glutamine synthetase I (GS I) was induced in Rhizobium japonicum 1-23 grown on glycerol as a sole source of carbon. The enzyme was purified 28.8 fold by heat-treatment ammonium sulfate fractionation, DEAE-Sepharose ion-exchange chromatography, Cibacron-Blue F3GA affinity chromatography, and Ultrogel AcA 22 gel fractionation. The n and the isoactivity point of GS I were 4.5 and 7.53, respectively. Molecular weight of GS I was 600,000 composed of twelve identical 52,000 subunits. The activities of GS I were inhibited by 87.2% (7.14 mM of alanine) and 98.1% (5 mM of inosine). K_m for the substrate, glutamine, was 5.4 mM and V_(max) was 6.90 μ㏖/min/㎎.
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성하진 광주보건대학 1978 論文集 Vol.4 No.-
Results obtained from a series of experiments on cultural conditions of Rhodotorula glutinis for glutathione production, on extraction methods, and on purification of glutathione were as follows. To maximize the glutathione content in the cell mass of R. glutinis, addition of Mg ion by 0.05% and Ca ion 0.01% to the basal medium was favorable. The optimal concentration of methionine added to the medium was approximately 2.5×10^(-3)M. Boiling water extraction at 90℃ for 15 minutes and ultrasonic extraction at 60 kc for 15 minutes were favorable method of extraction. Highest yield was obtained when extracted with ultrasonic treatment and subsquent boiling water treatment. The final recovery rate after purification was increased from 49.3% to 57.1% by addition of sodium gluconate at a 0.02% level.
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성하진,권미향,임은정 한국농화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1
During the screening for anti-complementary activity from 10 kinds of edible mushrooms, an alkali extract of Agaricus bisporus showed the highest activity through the complement fixation test. The crude anti-complementary material(AB-O) from Agaricus bisporus was obtained by the alkali extraction using 1 N NaOH containing 5% urea(65℃), followed by methanol reflux, dialysis and lyophilization. The fraction AB-O showed potent anit-complementary and anti-tumor activity against sarcoma-180 injected mice. The fraction AB-O was divided into 5 fractions(AB-20, AB-40, AB-60, AB-80, AB-A) by gradual acetone precipitation. Among them fraction AB-20 having the highest activity and yield was found to contain 39% carbohydrate and 46% protein. The anti-complementary protein-bound polysaccharide AB-20 consisted of glucose, arabinose, xylose, galactose and mannose in a molar ratio of 6.49 : 1.98 : 1.24 : 1.00 : 0.71, respectively and its main component amino acids were alanine(20.59%), isoleucine(16.85%), glutamine+glutamic acid(14.12%) and leucine (13.83%). The anti-complementary activity of AB-20 was decreased greatly by periodate oxidation, but decreased slightly by pronase digestion. This indicates that polysaccharide moiety is corelated with the anti-complementary activity and that protein is also involved in the activity.
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