마산만의 환경학적 연구 1. 물리적 특성과 화학 성분함량에 대하여

김종만,한상준,이종화,Kim, Jong Man,Han, Sang Joon,Lee, Jong Wha 한국해양학회 1976 韓國海洋學會誌 Vol.11 No.1

The physical factors and chemical contents were studied at 8 stations within Masan Bay, differing in depth and location with respect to inner and outer harbours. It is a relatively small bay with a long and narrow outlet. The water temperature and salinity at the inner bay was rather subject to change on weather condition of the neighbouring land than on the effects of the water mass of outer bay. The high nutrient contents in the bay were caused by the sewage and industrial activities. The outer harbour was less polluted than that of the inner bay; the high contents were significantly decreased from the entrance of the outer harbour. While the high disolved oxygen contents, over saturation in the surface strata may be the result of nearly all year round phytoplankton blooms, the lack of oxygen contents in the bottom strata were caused by the inadequate mixing of water mass and organic matters. The frequent red tide in the area may be the results of inflowing raw sewage, industrial activities of neighbouring land and inadequate mixing of water masses.

종자의 크기가 묘삼의 생육에 미치는 영향

김종만,이성식,김요태,Kim, Jong-Man,Lee, Seong-Sik,Kim, Yo-Tae 고려인삼학회 1981 Journal of Ginseng Research Vol.5 No.2

Ginseng seeds were gathered from 3,4,5 and 6 years of age and were classified into four qroups (below 4mm, 4∼5mm, 5∼6mm and above 6mm in across sieve). They were sown in seedling bed and some characters were investigated in each qroup of seed size. 1. The distribution of seed size of below 4mm, 4-5mm, 5-6mm and 6mm were 23.7%, 60.8%, 12.4% and 4.5%, respectively. 2. The ratio of seed coat dehiscence was not affected by seed size but emergence ratio and emerging vigor were superior in large seed. 3. The large seed showed superiority in stem length, stem diameter, leat and also in root length, root diameter and root weight. but diseased root was not affected by seed size. The effect of age(seed harvest) was not significant on all those characters.

묘삼의 표질이 본답에서의 생육에 미치는 영향 제2보. 묘삼의 중량과 본답에서의 인삼생육과의 관계

김종만,이성식,김요태,Kim, Jong-Man,Lee, Seong-Sik,Kim, Yo-Tae 고려인삼학회 1981 Journal of Ginseng Research Vol.5 No.2

This experiment was carried out to inverstigate the characteristics of growth of ginseng Plant on field conditions as affected by seedling weight. Weight of seedling ranged from 0.4 to 1.8g were divided into seven classes at interval of 0.2g. The heavy seedlings showed superiority in stem length, stem diameter, leaf area, number of palmate leaf and leaflets and root weight in 2-,3-, and 4-age as compared to light ones. The heavy seedlings also showed superiority in the ratio of inflorescence appearance in 2-age and seed product in 4-age as compared to light ones, but there were no significant difference in the ratio of missing-plant and the cracking. of root. The differences of growth in field condition as affected by seedling weight were diminished as the age progressed, and the annual growth rates of root of light seedling were high in older plant.

가축의 설사변에서 분리한 대장균과 살모넬라균의 항균물질 감수성과 마우스에서의 치료효과

김종만,진남섭,김종완,진영화,이희수,권창희,우승룡,이해천,박종명,김재학,이재진,Kim, Jong-man,Jean, Nam-seop,Kim, Jong-wan,Jean, Young-hwa,Lee, Hee-soo,Kweon, Chang-hee,Woo, Sung-ryong,Lee, Hae-chon,Park, Jong-myung,Kim, Jae-hak,Rhee, J 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.2

Survey on MIC of antimicrobial drugs and its treatment efficacy in mice were conducted for the strains of Escherichia coli and Salmonella spp isolated from feces of young domestic animals with diarrhea in 1996. A total of 338 strains of E coli and 61 strains of Salmonella spp were examined for the susceptibility to 20 antibiotics and 7 synthetic antimicrobial drugs. The results indicated that the majority of strains were susceptible to amikacin(93.5%), cefoperazone/sulbactam(93.5%), cefotaxim(93.3%), cefomandole(92.8%), cefoperazone(91.6%) and ciprofloxacin(85.1%), in order. Although gentamicin, ciprofloxacin and norfloxacin showed the relatively low MIC distributions, erythromycin, doxycycline, sulfamethoxazole and oxytetracycline revealed the high MIC distributions to most of isolates. The $MIC_{90}$ of antimicrobials for E coli were > $62.5{\mu}g/ml$ in gentamicin, $2.0{\mu}g/ml$ in ciprofloxacin, $1.0{\mu}g/ml$ in norfloxacin, > $500{\mu}g/ml$ in erythromycin, $125{\mu}g/ml$ in doxycycline, > $1000{\mu}g/ml$ in sulfamethoxazole and > $250{\mu}/ml$ in oxytetracycline. In general, the MIC of E coli isolates was higher than that of Salmonella spp isolates. Although variation in synergism or additivity of antibiotic combinations were demonstrated, ampicillin-gentamicin was the most efficacious combination both against E coli and Salmonella spp with the fluctuation of 7.7-77.5%. In the experiment of treatment efficacy in mice, the highest survival ratio(83.3%) after challenge with pathogenic E coli and Salmonella typhimurium was detected in the group treated with gentamicin.

면역학적인 방법에 의한 한우와 유우의 요네병 발생조사

김종만,안종삼,우승룡,조동희,조윤상,박정문,윤용덕,장국현,Kim, Jong-man,Ahn, Jong-sam,Woo, Seung-roung,Jo, Dong-hee,Jo, Yun-sang,Park, Jeung-moon,Yoon, Yong-dhuk,Chang, Guk-hyun 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1

A immunological survey of paratuberculosis in dairy and Korean native cattles was conducted by enzyme linked immunosorbent assay(ELISA), complement fixation test(CFT), agar gel immunodiffusion test (AGID) and intradermal skin test(ID). Over all prevalence of pararuberculosis in cattles was 6.7%(109/1633) by ID, 7.5(205/2719) by AGID, 9.3% (245/2641) by CFT and 13.4%(363/2719) by ELISA. Prevalence in dairy cattle was higher than that of Korean native cattle. Of 70 ELISA-positive cattle, 23(28.6%) and 48(68.6%) cattles were classified as positive in the AGID and positive or suspect in CFT, respectively. Of 92 ELISA-suspect cattle, 32(34.9%) and 48(52.2%) cattles were classified as AGID-positive and CFT-positive or suspect, respectively. It was concluded that paratuberculosis is widespread in cattle of Korea.

백신의 이론과 효과적인 접종방법 -양계농장에서 백신의 효능을 증가시키기 위한 방향-

김종만,Kim, Jong-Man 대한양계협회 2010 월간 양계 Vol.42 No.8

송아지 대장균(大腸菌) Pilus Vaccine 개발(開發)에 관한 연구(硏究): II. 시험(試驗)백신의 면역원성(免疫原性) 및 방어효과(防禦效果)

김종만,윤용덕,박정문,김봉환,Kim, Jong-man,Yoon, Young-dhuk,Park, Jeung-moon,Kim, Bong-hwan 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.1

The oil emulsion and alhydrogel pilli vaccines were prepared from a strain(O9: K35, K99, F41) of enterotoxigenic Escherichia coli isolated from calves with diarrhea and their immunogenicity was tested in guinea-pigs, pregnant goats and cows. Serum antibody responses to K99 and F41 antigens in guinea-pigs given experimental oil and gel vaccines peaked at 4 and 6 weeks after vaccinations. At that time, the mean hemagglutination inhibition titers to K99 and F41 antigens in guinea-pigs given oil vaccine were 1:25 and 1:1, 218 and those given gel vaccine were 1:54 and 1:724 respectively. Agglutinin titers in pregnant goats given the oil vaccine were significantly higher(mean 1:2,347) compared to those of control group(mean 1:160). Less than 12.5% of goatlings from vaccinated goats developed scours compared to nearly 100% in control group after oral challenge with enterotoxigenic Escherichia coil within 24 hours after birth. The highest agglutinin titers of cow serum and colostrum and of the serum of calves 48 hours after birth from cows given oil vaccine were 1:256, 1:512 and 1:64 respectively. On the other hand, those titers of serum and colostrum and of the serum of nursing calves from nonvaccinated cows were 1:8, 1:16 and 1:20 respectively. The protective efficacy of the oil emulsion vaccine was 72.1% under field conditions. These results strongly indicated that the vaccine could be applied for protection of diarrhea caused by enterotoxigenic Escherichia coli in calves.

낙엽분말을 이용한 합판용 접착제의 증량에 관한 연구

김종만,박종열,이필우 ( Jong Man Kim,Jong Yeol Bark,Phil Woo Lee ) 한국산림과학회 1979 한국산림과학회지 Vol.42 No.1

It was planned and performed to study the possibility on the use of inexpensive and easily acquirable foliage powder, which processed by pulverizing after dried, instead of imported expensive wheat flour for the extending of plywood adhesives. Pine leaves of softwood trees, Poplar, Oak and Sycamore leaves of broad leaved species were selected and harvested to pulverize into the minute foliage powder. The harvested foliages from each selected species were pulverized into 40 mesh particles after dried at 100∼105℃ condition during 24 hours in drying oven. To compare the extending effect of plywood adhesives with these foliage powders 100 mesh wheat flour using at current plywood industry was also prepared. Foliage powder and wheat flour were extended into 10, 20, 30, 50 and 100% to the urea and phenol formaldehyde resin. After plywoods were processed by the above extending method shear strength of extended plywoods were analyzed and discussed. The results obtained at this study are as follows: 1) Among 10% extensions of urea formaldehyde resin plywood, dry shear strength of plywood extended by wheat flours was the highest and that of non-extended plywood the next. Plywood extended with foliage powder showed the lowest dry shear strength. The order of dry shear strength of plywoods extended by foliage powder was that of Oak foliage powder extension, the best, that of Sycamore, that of Pine, and that of Poplar. 2) Among 20% extensions of urea formaldehyde resin plywood, plywood extended by wheat flour showed the highest dry shear strength, and the next was plywood by Poplar foliage powder. All these two showed higher dry shear strength than non-extension plywoods. Except Poplar, dry shear strength of foliage powder extension plywoods was bad, but the order of dry shear strength of plywoods extended by foliage powder was Pine, Poplar and Oak. 3) In the case of 30% extensions of urea formaldehyde resin plywood, dry shear strength of wheat flour extension was the highest and non-extension the next. Dry shear strength of foliage powder extension plywoods was poor with a rapid falling-off in strength. 4) Among 50% and 100% extensions of urea formaldehyde resin plywood, only wheat flour showed excellent dry shear strength. In the case of foliage powder extension, low dry shear strength showed at the 50% extension of Pine and Poplar, and plywoods of 50% extension of Oak foliage powder delaminated without measured strength. All plywoods of 100% foliage powder extension delaminated, and then shear strength were not measured. 5) Among wet shear strength of 10% extensions of urea formaldehyde resin plywood, wheat flour extension was the highest as in the case of dry shear strength, and non-extension plywood the next. Except Poplar foliage extension, all foliage powder extension plywoods showed low shear strength. 6) Wet shear strength of plywoods of 20% extension lowered in order of non-extension plywood, plywood of wheat flour extension and plywood of foliage powder extension, but other plywoods of foliage powder extension except plywoods of Poplar and Oak foliage powder extension delaminated, 7) Wet shear strength of 30% or more extension of urea for madehyde resin plywood were weakly measured only at 30% and 50% extension of wheat flour, and wet shear strength of plywoods extended by foliage powder were not measured because of delaminating. 8) Dry shear strength of phenol formaldehyde plywoods extended by 10% wheat flour was the best, and shear strength of plywoods extended by foliage powder were low, but the order was Oak, Poplar, and Pine, Plywood of Sycamore foliage powder extension delaminated. 9) In the case of 20% extensions of phenol formaldehyde resin, dry shear strength of plywood extended by wheat flour was the best, but plywood of Pine foliage powder extension the next, and the next order was Oak and Poplar foliage powder. Plywood of Sycamore foliage powder extension delaminated. 10) Among dry shear strength of 3

부루세라 RB51 백신의 임신소에 대한 안전성

김종만,우승룡,이지연,정석찬,진영화,김재훈,권창희,윤용덕,유한상,Kim, Jong-man,Woo, Sung-ryong,Lee, Ji-youn,Jung, Suk-chan,Jean, Young-hwa,Kim, Jae-hoon,Kweon, Chang-hee,Yoon, Young-dhuk,Yoo, Han-sang 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.4

The safety of Brucella abortus strain RB51(SRB51) was investigated in dairy cows and Korean native cattle of 4~7th month of gestation. From experimentally inoculated cattle, 18 of 25 (72.0%) dairy cows, and 3 of 10 (30.0%) Korean native cattle were aborted or delivered premature fetus. There were no significant differences in the incidence of abortion depending on the inoculation route (intramuscular and subcutaneous) and dosages ($1{\times}10^9$, $2.8{\times}10^9$, and $4.0{\times}10^9$ CFU). The antibodies to the SRB51 were measured by a dot blot enzyme-linked immunosorbent assay. The highest titers to SRB51 were detected between 5~7 weeks after inoculation and the specitic antibody could be detected up to 28 weeks after inoculation. The SRB51 was isolated from amnio-allantoic fluid, bronchial lymph node, mammary gland, and supramammary lymph node in 5 of 25 dairy cows during 4 weeks after either abortion or delivery. Although SRB51 was isolated from 4 of 24 aborted fetus or normally delivered calves at parturition time, it was not isolated during 4 weeks afterward. Eleven of twentyfive dairy cows showed the endometritis and/or necrosis until 6 weeks after delivery, no lesions were seen at 8 weeks after delivery and uterus from control dairy cows. The results of present study revealed that SRB51 might induce the clinical signs of brucellosis in the pregnant cattle at 4~7th month of gestation.

개량안강망 어선 설계와 조업실태

김종만,Kim, Jong-Man 선박안전기술공단 1984 漁船 Vol.19 No.-