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      • Alkalophilic Bacteria B가 생산하는 Xanthine Oxidase저해제에 관한 연구

        배은경,김건희 덕성여자대학교 자연과학연구소 2005 자연과학 논문집 Vol.11 No.-

        For the development of new xanthine oxidase inhibitor, alkaophilic bacteria B producing xanthine oxidase inhibitor was isolated from soil. The xanthine oxidase inhibitor was isolated from the culture broth of alkalophlic strain B. This xanthine oxidase inhibitor was sequentially purified by centrefugation, ethyl acetate extraction, Silica gel column chromatography, ephadex LH 20-100 gel column chromatography and Prep HPLC. The instrumental analysis showed that this xanthine oxidase inhibitor had 14 hydrogens, 11 carbons, aromatic ring, aldehyde group, amino or amine group, glycolipid and ketose or oligosaccharide in its structure. Molecular weight of XOase inhibitor might be 187 daltons

      • KCI등재
      • KCI등재

        학교정신건강상담 교사직무연수 프로그램 평가 연구

        배은경 한국정서행동장애학회 2018 정서ㆍ행동장애연구 Vol.34 No.2

        This study aimed to implement the teacher training program(hereafter the program) for school-based mental health counseling for elementary school teachers and evaluate its effects. This program was developed to improve the teachers’ competence in school-based mental health counseling according to the procedure from the intervention research of Rothman and Thomas(1994); the program includes the education on children’s mental health and ADHD and the repeated practices of the skills to support the emotion control, conflict mediation, prosocial behaviors and problem-solving of/among children. The program was implemented for 27 elementary school teachers once a week(two hours per a session) for eight weeks between October and November in 2015; 12 teachers for experimental group and 15 for comparison group. As to the quantitative evaluation, pretest-posttest design was used for the non-equivalent group in quasi-experimental research design and as to the qualitative evaluation, the records related with the program were collected and analyzed. The results of the quantitative evaluation showed that the level of the knowledge about ADHD of teachers went up and their difficulties and burnout decreased. The results of the qualitative evaluation showed the subjective experiences that were acquired by the teachers such as the improvement of competence in counseling, the awareness of the changes of students and the strong or/and weak points of the program. The said results showed that the teacher training program for school-based mental health counseling was positively effective and the improved competence of the teachers in school-based mental health counseling would contribute to the healthy development and improvement of mental health of children. 본 연구의 목적은 초등교사를 대상으로 학교정신건강상담 교사직무연수 프로그램을 실행하고 그 영향을 평가하는 것이다. 본 프로그램은 Rothman과 Thomas(1994)의 개입연구(Intervention Research)에 기반하여 개발되었으며, 아동정신건강 및 ADHD지식 등에 대한 교육, 아동의 감정조절 및 갈등중재, 친사회적 행동 및 문제해결을 지원하는 기술을 반복 실연함으로써 교사의 학교정신건강상담 역량 향상을 지원하였다. 이를 위하여 프로그램은 2015년 10월∼11월에 걸쳐 매 120분간 주 1회로 8주간 실행되었으며, 초등교사 27명(실험집단 12명, 비교집단 15명)이 참여하였다. 양적 평가를 위하여 유사실험설계의 비동일집단 사전․사후검사 설계가 사용되었으며, 질적 평가를 위하여 프로그램 관련 기록물 등의 자료를 수집하고 분석하였다. 그 결과, 양적 평가에서는 본 프로그램에 참여한 초등교사의 ADHD지식이 증가하고, 상담의 어려움과 소진은 감소된 것으로 나타났다. 질적 평가에서는 초등교사의 상담역량 향상, 학급원의 변화 인식, 교사직무연수 프로그램의 강점 및 보완점 인식이라는 주관적 경험이 나타났다. 본 연구결과는 학교정신건강상담 교사직무연수 프로그램의 효과성을 뒷받침한다고 볼 수 있으며, 초등교사의 학교정신건강상담 역량 향상을 통하여 궁극적으로 아동의 건강한 발달 및 정신건강 증진에 기여할 수 있을 것으로 기대된다.

      • 오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교

        배은경,이인애,김기용,이병현,손대영,이효신,정민섭,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

        Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) × regeneration ratio(%)/100]for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93>Sparta>Pizza>Condor>Lidaglo>Glorus>Hapsung2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of φ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between φ2.5cm~4cm. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed sxplants for the best 6 varieties was Condor>Sparta>93M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between φ1.5~5.7cm in which Sparta, the largest one of φ5.7cm was five times larger than the smallest one. The callus size of the control variety. Hapsung 2 was φ3.8cm, which belonged to larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Plano while no callus was regenerated in Juno, 9. The range of regeneration effciency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration effciency were r=0.8369, r=0.6683 and r=0.5937, respectively in 6-week callus, and all the correlations were significant on the 1% level.

      • 체강액에서 총 Lactate dehydrogenase 및 Lactate dehydrogenase 동위효소 측정의 진단학적 의의

        김정숙,전창호,이영현,홍석일,배은경 영남대학교 의과대학 1986 Yeungnam University Journal of Medicine Vol.3 No.1

        1984년 10월부터 1986년 5월까지 영남대병원 임상병리과에 의뢰된 132예의 복수액과 늑막액의 진단적가치를 설정하기위한 연구로서 총 LD, LD동위효소의 활성도, 생화학 및 혈액학적 검사, 세포검사 등의 소견을 연구한 결과 다음과 같은 결론을 얻었다. 1. 복수액은 총 LD활성도 및 단백양으로 여출액의 감별이 가능하였다. 2. 복수액과 늑막액의 악성삼출액은 LD2가 염중성 삼출액에 비하여 증가되었고(P<0.001), 혈청에 비하여 LD4가 증가되어 (P<0.05)통계적 유의성을 나타내었다. 3.늑막액과 복수액의 염증성 삼출액은 LD5가 증가하여 혈청과 악성삼출액에 비하여 의의성 있는(P<0.05)증가를 나타내었다. 4. 복수액에서는 염증성 삼출액의 총 LD양은 악성삼출액에 비해 유의한 증가를 보여 총 LD활성도에 의한 삼출액의 구별이 가능하였으나 늑막액에서는 악성 삼출액과 결핵성 삼출액의 총 LD활성도 차이의 통계적 유의성은 없었다. 5. 총 LD 및 LD5동위효소의 삼출액에서 백혈구수와 상관관계가 성립하지 않았다. Body fluid Lactate dehydrogenase and its isoenzyme measurment was perfomed in 132 patients : 8 cases with peritonitis, 21 cases with malignant ascites, 43 cases with liver cirrhosis, 48 cases with tuberculous pleuritis, 12 cases with malignant pleural effusion respectively. Body fluid protein and glucose contents, red blood cell counts, white blood cell counts, cytologic examination were also performed as a comparatve study. The results were as follows : 1. Measurement of total LD and protein amount could differentiate between transudate and exudate in the ascitic fluids. 2. In the malignant exudate of ascites and pleural fluid, the activity of LD2 isoenzyme was statistically increased compared with that of inflammatory exudate and the activity of LD4 isoenzyme was also increased compared with that of serum(P<0.05). 3. The inflammatory exudate of pleural fluid and ascites demonstrated the increase of LD5 isoenzyme activity statistically compared with that of serum and malignant exudate(P<0.05). 4. A difference of total LD activity between malignant ascites and inflammatory ascites was significant statically, while this was not observed in the pleural exudate. 5. Total LD and LD5 isoenzyme activity didn't correlated with the number of white blood cells in the exudate.

      • KCI등재

        탄광부 진폐증환자의 Angiotensin-Converting Enzyme활성치

        김경동,최명숙,이채훈,김정숙,배은경 영남대학교 의과대학 1989 Yeungnam University Journal of Medicine Vol.6 No.1

        1988년 9월부터 1989년 4월 까지 문경병원에서 탄광부 진폐증으로 진단받고 입원 중인 환자 75명과 정상대조군 100명을 대상으로 영남대학병원 임상병리과에서 실시하고 있는 ??법에 의한 혈중 ACE치를 분석하여 다음과 같은 결론을 얻었다. 1. 정상대조군의 혈중 ACE치(평균±표준편차)는 남녀 각각 13.6±3.3U/L, 13.2±4.3U/L 이었으며, 성별 및 연령별 차이는 없었으며, 정상대조군 전체의 결과는 13.4±3.9U/L로 나타나 참고범위는 4.6-21.8U/L로 나타났다. 2. 용수법으로 시행한 ??법의 일내변이는 5.6%이하, 일간변이는 6.5%이하로 나타나 비교적 안정된 분석치를 나타냈다. 3. 탄광부 진폐증 환자군의 혈중 ACE치(평균±표준편차)는 20.3±5.7U/L로서 정상 대조군보다 유의한 상승을 나타냈다(P<0.01). 혈중 ACE치의 분석은 탄광부 진폐증 환자의 경과 관찰이나 진단방법의 일환으로 이용될 수 있을 것으로 사료되는 바, 향후 이 방면의 추적 연구가 필요할 것으로 사료된다. We measured fasting serum angiotensin-converting enzyme (SACE) in 100 healthy controls and 75 coal worker's pneumoconiosis (CWP) patients by a commercial kits(??, Fujirio Inc., Japan) and evaluated this manual method. The linear range extends to an activity of 80U/L. Precision on a commercial control serum (ACE control-??, Sigma Co.) with a mean value of 9.47U/L yielded a within-run and between-run CVs are 5.6% (N=15) and 6.9% (N=14) respectively. SAVE in 75 CWP was 20.3±5.7U/L (mean±s.d.) ; higher than in healthy controls(13.4±3.9U/L, P<0.01). No correlation was found between SACE, sex, and age. The results suggest that the measurement of SACE and follow-up SACE in coal workers may be a useful diagnostic tools for CWP.

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