Amplification of the UQCRFS1 Gene in Gastric Cancers

전경화,김수영,윤정환,Jae Hwi Song,박원상 대한위암학회 2012 Journal of gastric cancer Vol.12 No.2

Purpose: The specific aim of this study is to unravel a DNA copy number alterations, and to search for novel genes that are associated with the development of Korean gastric cancer. Materials and Methods: We investigated a DNA copy number changes in 23 gastric adenocarcinomas by array-comparative genomic hybridization and quantitative real-time polymerase chain reaction analyses. Besides, the expression of UQCRFS1, which shows amplification in array-CGH, was examined in 186 gastric cancer tissues by an immunohistochemistry, and in 9 gastric cancer cell lines, as well as 24 gastric cancer tissues by immunoblotting. Results: We found common gains at 48 different loci, and a common loss at 19 different loci. Amplification of UQCRFS1 gene at 19q12 was found in 5 (21.7%) of the 23 gastric cancers in an array-comparative genomic hybridization and DNA copy number were increased in 5 (20.0%) out of the 25 gastric cancer in quantitative real-time polymerase chain reaction. In immunohistochemistry, the overexpression of the protein was detected in 105 (56.5%) out of the 186 gastric cancer tissues. Statistically, there was no significant relationship between the overexpression of UQCRFS1 and clinicopathologic parameters (P>0.05). In parallel, the overexpression of UQCRFS1 protein was confirmed in 6 (66.7%) of the 9 gastric cancer cell lines, and 12 (50.0%) of the 24 gastric cancer tissues by immunoblotting. Conclusions: These results suggest that the overexpression of UQCRFS1 gene may contribute to the development and/or progression of gastric cancer, and further supported that mitochondrial change may serve as a potential cancer biomarker.

발달장애아 부모의 생애사를 통해 본 도전적 행동 돌봄 적응

전경화,임병우,이형주,김신호,김혜경 경인행정학회 2019 한국정책연구 Vol.19 No.3

본 연구는 발달장애 자녀를 둔 부모들이 자녀의 도전적 행동으로 인해 당면하게 된 돌봄 관련 적응과정을 심도 있게 이해하기 위한 탐색적 연구이다. 연구방법으로는 질적 연구 방법 중의 하나인 생애사 연구로써 Mandelbaum(1973)의 삶의 영역(Dimensions), 전환점(turnings), 적응(adaptation)’이라는 개념 틀로 분석하였다. 본 연구결과에 따르면, 도전적 행동을 하는 자녀를 둔 부모들은 신체적 스트레스, 정서적 피폐, 사회적 고립, 경제적 어려움 등의 복합적인 문제들을 경험한 것으로 나타났다. 구체적으로 삶의 영역에서는 자녀가 장애라는 낙인을 갖고 태어난 것에 대한 원망과 분노, 불안을 삭힐 여유도 없이 돌발적이고 공격적인 행동을 하는 자녀를 양육하며 어떠한 돌파구를 찾아야 했던 참여자들의 생애사적인 흐름 속에서 그들이 갖고 있는 생각들을 볼 수 있었다. 태어난 아이의 잘못도 돌봄자인 부모의 잘못도 아닌데 겪어야 하는 문제 상황들을 감내해야 했다. 전환점에서는 이러한 도전적 행동에 대한 돌봄 스트레스, 동질집단에서의 갈등, 가족갈등, 사회적 단절과 소외, 양육 혼란 속에서 하루하루를 살아내야 했고, 이러한 상황은 자녀의 미래에 대한 불안으로 나타났다. 자녀의 도전적 행동은 예고를 하지 않으므로 때로는 자녀에게 맞기도 하고, 타인에게 해를 입히기도 하는 과정 속에 남편, 가족과 갈등이 발생하며, 심지어는 가족이 해체되는 결과를 낳기도 하였다. 이러한 스트레스 상황은 고령기에 다가갈수록 주돌봄자와 장애 자녀에게 무거운 현실로 다가온다. 이러한 삶의 무게를 지탱하려면 누군가의 도움이 반드시 필요하다. 적응의 영역에서 참여자들은 자녀의 행동이 생존하기 위한 행동임을 알게 되었고, 자녀의 장애를 수용하며 사회에서 살아갈 길을 찾게 된다. 이러한 과정에서 가족의 소중함을 알게 되었고 사회에서 자녀와 함께 살아가기 위한 대변자의 역할을 하게 된다. 부모의 가장 큰 걱정은 부모 사후에 자녀가 살아갈 세상이다. 이를 위해 단절되었던 이웃, 더 나아가 지역사회에 문을 두드리며 부정적인 장애인식을 변화시키려고 노력하게 되었다. 본 연구자는 이와 같은 결과를 바탕으로 다양한 실천적 함의를 제시하였다. The research explores in-depth understanding of the process of parents with developmental disability children, caring about children's future and confronting parental caring stress, family conflict, struggle with homogenous group, and social alienation due to their challenging behavior. This study is a qualitative research approaching life story research by Mandelbaum's(1973) concept frame Dimensions, Turnings, and Adaptation. According to the research result, parents with developmental disability children have experienced physical stress, mental exhaustion, social isolation, economical difficulties and complex problems by their challenging behavior. Concretely, in dimensions, the participants had to look for any breakthrough for their children's challenging behavior without being able to reduce their resentment, anger and anxiety for born with disablility. The participants reflected their thoughts through daily routine. Neither was the fault of the born child nor the caregiver parents' fault, yet had to endure every situation. In Turnings, by cause of challening behavior the participants had to cope up every caring stress, struggle with homogenous group, family conflict, social alienation and nurture. These circumstances appeared to be anxious about the future of their children. Challenging behavior is not foreseeable and is possible to receive violence, furthermore gives violence to others. In the process of these actions conflict happens between family, even causing family separation. This stressful situation comes to heavy reality for caregivers and their children as they get older. To support this weight of life, someone's help is essential. In the Adaptation, the participants realize their behavior is to survive, moreover accept their children's disability and find ways to live in the society. In this process the participants learn the value of family and become spokesperson to live the society. The biggest concern for parents is the world in which their children live after they have left the world. To do this, they knocked on the neighbors who were cut off and even the community, trying to change the negative perception of disability. Based on these results, the researcher suggested various practical implications.

세계화에 대비한 유아기 전통 교육에 대한 고찰

전경화 부천대학 1999 論文集 Vol.20 No.-

In the age of globalization which is characterized by its openness, the cultural pluralism requires an attitude that is willing to understand and accept other country's cultures. In order to prepare for the 21st century's globalization and to obtain their own identity of traditional cultures, education on tradition has to be done from the age of childhood. In this paper, we investigated the current status of our childhood education on tradition and the problems of it that has been made from the western point of view. We also suggested a desirable direction of kindergarten's education on tradition based on the investigation. The results are as follows: 1. The tradition of each country is the culture that has been inherited for a long time from their own life. It cannot be classified as "Good" or "Bad." 2. Tradition is what is alive at present, not the traditum of museum. 3. The education on tradition has to be made for national identity as well as natioal survival. 4. The childhood education on tradition has to be extended to not simply traditional play but our traditional view to the world, culture of life, and the culture of museum. 5. For the efficient education on tradition, various programs must be developed which include an education utilizing cultural museum, an education using fairy tales, and an education harnessing picture books of great men of past ages.

교육실습을 통한 예비유아교사의 교사 효능감 변화 탐색

전경화,허윤정,한남주 한국영유아교원교육학회 2006 유아교육학논집 Vol.10 No.4

Silk Fibroin Composite Hydrogels Prepared by Enzymatic Crosslinking System

전경화,김정수,이현지,김지환,양세준,이기훈 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.2

고령 장애인의 체력단련 프로그램 이용 경험에 관한 질적 연구

전경화,임병우 한국노년학연구회 2017 한국 노년학연구 Vol.26 No.2

DNA Methylation of Multiple Genes in Gastric Cancer: Association with CpG Island Methylator Phenotype and Helicobocter pylori Infection

전경화,원용성,신은영,조현민,임명구,진형민,박우배,Jun, Kyong-Hwa,Won, Yong-Sung,Shin, Eun-Young,Cho, Hyun-Min,Im, Myoung-Goo,Chin, Hyung-Min,Park, Woo-Bae The Korean Gastric Cancer Association 2006 대한위암학회지 Vol.6 No.4

목적: 유전자 메틸화는 유전자의 서열에 영향을 주지 않으면서 유전자의 발현을 억제하고 세포분열 후 그대로 보존되는 후성적 변화이다. 위암조직과 정상위조직에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin 유전자와 MINT (MINT1, 2, 12, 25, 31)의 메틸화 상태를 검사하여 위암의 발생 과정에서의 작용과 CIMP 및 Helicobacter pylori균 감염을 포함한 임상병리학적인자와의 연관성을 알아보고자 하였다. 대상 및 방법: 위암과 정상위 신선 동결 조직 각각 36예를 대상으로 MSP (methylation-specific PCR)방법을 이용하여 메틸화 상태를 분석하였고 CIMP의 분석은 MINT1, MINT2, MINT12, MINT25, MINT31의 5개 marker를 대상으로 시행하였다. Helicobacter pylori균 감염여부는 Warthin-Starry silver 염색을 통하여 분류하였다. 결과: 위암 관련 유전자인 p14, p16, MGMT, COX-2, E-cadherin, hMLH1의 메틸화는 각각 14예(38.9%), 13예(36.1%), 8예(22.2%), 10예(27.8%), 21예(58.3%), 6예(16.7%)였다. MINT1과 MINT25의 메틸화는 위암조직에서 정상위조직에서보다 통계학적으로 유의하게 높게 관찰되었다. CIMP 양성률은 위암조직에서 44.4%로 높게 나타났으며 CIMP-H 위암은 환자의 연령과 종양크기와 연관이 있었다. CIMP 양성 위암은 p16 유전자의 메틸화와 연관이 있었고 p16 유전자의 메틸화는 조직학적으로 저분화, 미만형, 궤양형성하는 위암에서 낮게 나타났다. MINT1의 메틸화는 Helicobacter pylori균과 연관성이 있었다. 결론: 위암에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT (MINT1, 2, 12, 25, 31)의 불활성화에 DNA 메틸화가 작용함을 알 수 있었고, Helicobacter pylori균에 의한 위암발생에 MINT1의 메틸화가 연관이 있음을 알 수 있었다. Purpose: Methylation of gene regulatory elements plays an important role in gene inactivation without genetic alteration. Gastric cancer is one of the tumors that exhibit a high frequency of CpG island hypermethylation. The purpose of this study was to investigate the occurrence of CpG island hypermethylation in gastric carcinoma in relation to H. pylori infection, CIMP and clincopathologic variables. Materials and Methods: We investigated the promoter methylation Status of six genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin) and CIMP in 36 gastric carcinoma tissues as well as in nontumor tissues. CIMP status was investigated by examining the methylation status of MINT 1, 2, 12, 25 and 31. The methylation status of the promoter was examined by methylation-specific PCR (MSP) and H. pylori infection was examined by histological diagnosis after staining with Warthin-Starry silver. Results: Among the 36 gastric carcinoma tissues, DNA hypermethylation was detected in the following frequencies: 14 (38.9%) for p14, 13 (36.1%) for p16, 8 (22.2%) for MGMT, 10 (27.8%) for COX-2, 21 (58.3%) for E-cadherin, and 6 (16.7%) for hMLH1. The frequencies for MINT1 and MINT25 hypermethylation were significantly higher in tumor tissues than in nontumor tissues. 16 (44.4%) of the 36 gastric carcinoma tissues were positive for the CIMP CIMP-H tumors were associated with older patients and larger tumor size than CIMP-L tumors. We found a significant association between the presence of the CIMP and hypermethylation of p16. Hypermethylation of p16 and MINT2 were significantly different when compared by age. MINT1 gene methylation was significantly associated with H. pylori infection (P=0.004). Conclusion: Our results suggest that aberrant hypermethylation of multiple tumor related genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT1, 2, 12, 25, 31) occurs frequently in gastric carcinoma tissues. The hypermethylation of MINT1 was significantly higher in the tumor tissues and was associated with H. pylori infection.

Enzymatically cross-linked silk fibroin hydrogels triggered by glucose

전경화,이기훈,김정수,이현지,김지환 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.1

부모의 국가정체성과 교육적 태도 및 유아의 국가정체성에 대한 연구

전경화,강정원 한국영유아교원교육학회 2004 유아교육학논집 Vol.8 No.3

The purpose of this study is to collect get fundamental data for effective cultural education involving parents, children, and kindergartens. We hope to clarify and correlate the perception of traditional culture and the spirit of nationalism shared by parents and children alike. The perception of the importance of traditional culture and spirit of nationalism has been negatively correlated with both parents in nuclear families coming from mega cities, having a family income below the mean, and having a low level of academic achievement. Because of these correlations, it has been deemed necessary to draw up a concrete program that provides parents and children with joint traditional cultural experiences through a partnership of school and home. 본 연구는 가족의 인구학적 배경에 따른 국가 정체성과 교육적 태도 및 유아의 국가정체성의 관계를 알아보고, 유아교육기관과 부모와의 협력적 전통문화교육 프로그램 구성에 필요한 기초 자료를 얻고자 한다. 연구 결과, 부모 모두 대도시에서 자란 경우, 수입이 평균이하인 경우, 핵가족인 경우, 부모의 학력이 낮은 경우에 국가정체성이 낮은 경향을 보였고, 이러한 변인들은 유아의 국가정체성에도 영향을 미치는 것으로 나타났다. 그러나 부모와 유아의 국가 정체성( 전통문화인식과 국가 자긍심)에는 상관이 적은 것으로 나타나 부모 자녀 간에 전통문화 경험은 부족한 것으로 보인다. 따라서 가정에서도 전통문화에 대한 인식과 교육의 실제가 이루어지도록 돕는 구체적인 방안이 필요하다.

교육 실습의 실태 분석및 개선방향

전경화 부천대학 1990 論文集 Vol.11 No.-