김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

Is step section necessary for determination of complete pathological response in rectal cancer patients treated with preoperative chemoradiotherapy?

Park, Seog Yun,Chang, Hee Jin,Kim, Dae Yong,Jung, Kyung Hae,Kim, Sun Young,Park, Ji Won,Oh, Jae Hwan,Lim, Seok‐,Byung,Choi, Hyo Seong,Jeong, Seung‐,Yong Blackwell Publishing Ltd 2011 Histopathology Vol.59 No.4

<P>Park S Y, Chang H J, Kim D Y, Jung K H, Kim S Y, Park J W, Oh J H, Lim S‐B, Choi H S & Jeong S‐Y
(2011) <I>Histopathology</I><B>59</B>, 650–659</P><P><B>Is step section necessary for determination of complete pathological response in rectal cancer patients treated with preoperative chemoradiotherapy?</B></P><P><B>Aims: </B> To assess the efficacy of the step section for determination of pathological complete response (pCR) in rectal cancer treated with preoperative chemoradiotherapy (CRT).</P><P><B>Methods and results: </B> Of 709 patients with rectal cancer who received preoperative CRT, 88 were initially diagnosed as having pCR. These 88 patients were re‐evaluated after two‐level step sections of the entire tumour by using Dworak’s regression grade. Additional serial step sections revealed residual tumour cells in seven of 88 patients (7.95%), all of whom were upgraded to regression grade 3 (near total regression) from regression grade 4 (total regression). Of these seven patients, one (14.3%) showed tumour recurrence, compared with 11 of 81 (13.6%) patients with a final regression grade of 4. Neither recurrence rate nor disease‐free survival rate differed significantly between these two groups (<I>P </I>><I> </I>0.5). Calcification was significantly more frequent in grade 3 than in grade four patients (71.4% versus 32.1%; <I>P </I>=<I> </I>0.037), and acellular mucin pools were associated with better disease‐free survival (<I>P </I>=<I> </I>0.022).</P><P><B>Conclusions: </B> Stratifying patient outcome by final regression grade after step section did not yield different outcomes in patients with initial pCR. If residual tumour cells are not identified on initial meticulous examination, further processing of step sections is not necessary.</P>

혈압 조절이 당뇨병성 만성 신부전증의 진행속도에 미치는 영향

박인석 ( Park In Seog ),박주현 ( Park Ju Hyeon ),이승헌 ( Lee Seung Heon ),안석주 ( An Seog Ju ),양철우 ( Yang Cheol U ),김석영 ( Kim Seog Yeong ),장윤식 ( Jang Yun Sig ),윤영석 ( Yun Yeong Seog ),손호영 ( Son Ho Yeong ),방병기 ( 대한내과학회 1993 대한내과학회지 Vol.44 No.6

연구 배경 : 당뇨병성 신증은 신 대치요법을 필요로 하는 주요 원인이며, 그 빈도가 증가추세에 있고, 말기 신부전으로의 진행이 필연적이므로 사구체의 손상에 대한 위험 요소들을 조절하여 신부전의 진행속도를 저하시키려는 노력이 필요하다. 그 중 고혈압의 조절이 그 진행속도를 늦출 수 있다는 여러 임상보고 및 동물실험의 증거가 있으나, 적정 혈압의 목표에 대해서는 알려져 있지 않다. 이에 저자들은 혈압 조절이 당뇨병성 만성 신부전증의 진행속도에 미치는 영향을 관찰하였다. 방법 : 가톨릭 의과대학에서 당뇨병성 만성 신부전증으로 진단받고 보존적인 치료를 받고 있는 6개월 이상 추적이 가능했던 환자 30명을 대상으로 내원시마다 측정된 혈압과 혈청 크레아티닌을 조사하였다. 또한 추적기간을 평균동맥압에 따라 3개의 period로 나누고 각 peirod에서의 신기능의 진행속도를 혈청 크레아티닌의 역수와 시간과의 상관계수로 계산하였다. 결과 : 혈청 크레아티닌의 증가치와 그 역수의 감소로 본 평균 동맥압에 따른 신부전증의 진행속도는 각각 A period(평균동맥압?105mmHg)에서 0.0797±0.0286mg/dl/month, -0.0079±0.0018dl/mg/month, B period(105<평균동맥압<115mmHg)에서0.2224±0.0976mg/dl/month, -0.0430±0.0093dl/mg/month, C period(평균동맥압?115mmHg)에서 0.2731±0.0452mg/dl/month, -0.0434±0.0073dl/mg/month였으며, 모두 평균동맥압이 105mmHg 이하일때 의미있게 신부전의 진행속도가 감소되었다(p<0.0). 결론 : 이상의 성적으로 보아 당뇨병성 만성 신부전증환자에서 평균 동맥압을 적어도 105mmHg 이하로 유지하는 것이 신부전의 진행속도를 감소시키는데 중요하다고 사료된다. Background : Dabetic nephropathy is considered as the main cause of end stage renal disease which needs the renal replacement therapy. The adequate control of blood pressure is known important to reduce the progression rate of renal function in patients with diabetic renal insufficiency. We investigate the effect of blood pressure control on the progression rate of renal function in 30 patients with diabetic renal insufficiency. Method : We divided the observation time to 3 periods arbitarily according to degree of blood pressure control. The progression rate of renal function was measured by the monthly changs of serum creatinine concentration and reciprocal serum creatinine concentration. Result : The monthly changes in serum creatinine concentration in A period (MBP?105mmHg), B period (105mmHg<MBP?115mmHg) and C period (MBP?115mmHg) were 0.0797±0.0286, 0.2224±0.0976 and 0.2731±0.0452mg/dl/month, respectively. The monthly changes in reciprocal serum creatinine concentrations in A, B, C period were -0.0079±0.0018, -0.0430±0.0093 and -0.0434±0.0073dl/mg/month respectively. The monthly changes in serum creatinine concentrations and reciprocal serum creatinine concentrations in A period were significantly low compared with B and C periods. Conclusion : The results may suggest that adequate control of mean blood pressure below 105mmHg is important to reduce the progression rate of renal function in patients with diabetic renal insufficiency.

Selective GSK-3β inhibitors attenuate the cisplatin-induced cytotoxicity of auditory cells

Park, Hee-Je,Kim, Hyung-Jin,Bae, Gi-Sang,Seo, Sang-Wan,Kim, Do-Yun,Jung, Won-Seok,Kim, Min-Sun,Song, Mi-Young,Kim, Eun-Kyung,Kwon, Kang-Beom,Hwang, Sung-Yeon,Song, Ho-Joon,Park, Cheung-Seog,Park, Rae- Elsevier 2009 Hearing research Vol.257 No.1

<P><B>Abstract</B></P><P>Glycogen synthase kinase-3 (GSK-3) plays an important role in the regulation of apoptosis. However, the role of GSK-3 in the auditory system remains unknown. Here we examined whether the GSK-3-specific inhibitors, SB 216763 and LiCl, could protect against cisplatin-induced cytotoxicity of auditory cells. GSK-3 was activated by cisplatin treatment of HEI-OC1 cells. SB 216763 or LiCl treatments inhibited cisplatin-induced apoptosis in a dose-dependent manner and activated caspase-9, -8 and -3. In rat primary explants of the organ of Corti, SB 216763 or LiCl treatments completely abrogated the cisplatin-induced destruction of outer hair cell arrays. Administration of SB 216763 or LiCl inhibited cochlear destruction and the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in cisplatin-injected mice. Furthermore, administration of SB 216763 or LiCl reduced the thresholds of the auditory brainstem response (ABR) in cisplatin-injected mice. Collectively, these results suggest that cisplatin-induced ototoxicity might be associated with modulation of GSK-3 activation.</P>

Comparison of CpG island hypermethylation and repetitive DNA hypomethylation in premalignant stages of gastric cancer, stratified for Helicobacter pylori infection

Park, Seog-Yun,Yoo, Eun J,Cho, Nam-Yun,Kim, Nayoung,Kang, Gyeong H John Wiley Sons, Ltd. 2009 The Journal of pathology Vol.219 No.4

<P>CpG island hypermethylation and genomic DNA hypomethylation are found not only in gastric cancers but also in associated premalignant lesions. Helicobacter pylori infection induces aberrant CpG island hypermethylation in gastric mucosae. However, little is known about the relationship between H. pylori infection and aberrant methylation in premalignant lesions. The present study characterized methylation changes in a subset of genes and repetitive DNA elements (ALU, LINE-1, SAT2) and examined their relationship with H. pylori infection in premalignant lesions of gastric cancers. We performed MethyLight analysis of 25 genes and SAT2 and COBRA analysis of ALU and LINE-1 in 212 gastric tissue samples. H. pylori infection was closely associated with enhanced hypermethylation of CpG island loci in chronic gastritis samples, but this association was not found among intestinal metaplasias, gastric adenomas and gastric cancers. The number of methylated genes was greater in intestinal metaplasia and gastric adenoma samples than in chronic gastritis samples, regardless of H. pylori infection. Methylation of repetitive DNA elements in gastric lesions generally decreased with progression of the gastric lesion along the multistep carcinogenesis. No difference was noted in the number of methylated genes in chronic gastritis or intestinal metaplasia between gastric cancer patients and non-cancer subjects. In conclusion, we found that there was no enhanced CpG island hypermethylation in gastric cancer and premalignant lesions in association with H. pylori infection and our findings suggest that CpG island hypermethylation and repetitive DNA hypomethylation are enhanced with progression of the gastric lesion through the multistep carcinogenesis, regardless of the status of H. pylori infection. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.</P>

Identification of occult tumors by whole-specimen mapping in solitary papillary thyroid carcinoma

Park, Seog Yun,Jung, Yuh-S,Ryu, Chang Hwan,Lee, Chang Yoon,Lee, You Jin,Lee, Eun Kyung,Kim, Seok-Ki,Kim, Tae Sung,Kim, Tae Hyun,Jang, Jeyun,Park, Daeyoon,Dong, Seung Myung,Kang, Jae-Goo,Lee, Jin Soo,R Society for Endocrinology 2015 Endocrine-related cancer Vol.22 No.4

<P>We undertook this study to estimate an accurate incidence and spread patterns of occult papillary thyroid carcinoma (PTC) in patients with a preoperative diagnosis of solitary PTC by using whole-specimen mapping of all specimens after a total thyroidectomy. Enrolled prospectively in this whole-thyroid mapping study are 82 consecutive patients who underwent a total thyroidectomy under a preoperative diagnosis of solitary PTC. All thyroidectomy specimens were serially sectioned in 2 mm thickness and whole-thyroid mapping was carried out for additional foci of occult PTC. The frequencies of occult lesions detected in the whole and contralateral lobe were determined, and clinicopathologic factors associated with multifocality were assessed. Whole-thyroid mapping revealed 66 occult PTC lesions missed by preoperative ultrasound in 37 (45.1%) of the 82 patients. The great majority (92.5%) of the occult PTC was smaller than 3 mm in size and 25 patients (30.5%) had contralateral lesions. We found that the male sex was an independent predictor of multifocality (odds ratio (OR), 3.00; 95% CI, 1.11–8.14), adjusting for preoperative findings. Analysis with pathologic parameters showed that the male sex (OR, 5.03; 95% CI, 1.68–15.08) and extrathyroidal extensions (OR, 3.03; 95% CI, 1.03–8.95) were associated with multifocal PTC. However, none of the clinicopathologic factors evaluated predicted contralateral PTC. Our study demonstrates the diagnostic limitations of ultrasound for the detection of multifocal PTC and the need to consider the possibility of occult lesions in the management of solitary PTC, especially in male patients.</P>

Methylation Profiles of CpG Island Loci in Major Types of Human Cancers

Park, Seog-Yun,Kim, Baek-Hee,Kim, Jeong Ho,Cho, Nam-Yun,Choi, Minhee,Yu, Eun Joo,Lee, Sun,Kang, Gyeong Hoon The Korean Academy of Medical Sciences 2007 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.22 No.2

<P>Several reports have described aberrant methylation in various types of human cancers. However, the interpretation of methylation frequency in various human cancers has some limitations because of the different materials and methods used for methylation analysis. To gain an insight into the role of DNA hypermethylation in human cancers and allow direct comparison of tissue specific methylation, we generated methylation profiles in 328 human cancers, including 24 breast, 48 colon, 61 stomach, 48 liver, 37 larynx, 24 lung, 40 prostate, and 46 uterine cervical cancer samples by analyzing CpG island hypermethylation of 13 genes using methylation-specific PCR. The mean numbers of methylated genes were 6.5, 4.4, 3.6, 3.4, 3.1, 3.1, 3.1, and 2.1 in gastric, liver, prostate, larynx, colon, lung, uterine cervix, and in breast cancer samples, respectively. The number of genes that were methylated at a frequency of more than 40% in each tumor type ranged from nine (stomach) to one (breast). Generally genes frequently methylated in a specific cancer type differed from those methylated in other cancer types. The findings indicate that aberrant CpG island hypermethylation is a frequent finding in human cancers of various tissue types, and each tissue type has its own distinct methylation pattern.</P>

Mixed-type gastric cancer and its association with high-frequency CpG island hypermethylation

Park, Seog-Yun,Kook, Myeong Cherl,Kim, Young Woo,Cho, Nam-Yun,Kim, Tae-You,Kang, Gyeong Hoon Springer-Verlag 2010 Virchows Archiv Vol.456 No.6

CpG island hypermethylator phenotype in gastric carcinoma and its clinicopathological features

Park, Seog-Yun,Kook, Myeong Cherl,Kim, Young Woo,Cho, Nam-Yun,Jung, Namhee,Kwon, Hyeong-Ju,Kim, Tae-You,Kang, Gyeong Hoon Springer-Verlag 2010 Virchows Archiv Vol.457 No.4

의성 마늘의 브랜드 마케팅 전략 개발

김윤호(Yun Ho Kim),박길석(Gil Seog Park),박주섭(Joo Sub Park),최칠구(Chil Koo Choi),박승용(Seung Yong Park) 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.5