Stilbenes and Oligostilbenes from Leaf and Stem of Vitis amurensis and Their Cytotoxic Activity

Do Thi Ha,Quan Cheng Chen,Tran Manh Hung,Tran Minh Ngoc,Phuong Thien Thuong,김홍진,성연희,민병선,배기환,윤의중 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.2

Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-ε -viniferin (3), gnetinH (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)- ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with IC50 values ranging from 15.7 ± 2.1 to 30.9 ± 1.8 μM. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 (IC50 values of 30.6 ± 4.1 and 28.7 ± 2.81μM, respectively) and K562 (IC50 values of 38.6 ± 0.82 and 24.6 ± 0.76 μM, respectively). Gnetin H (4)showed only weak cytotoxic activity against L1210 with an IC50 value of 40.1 ± 4.23 μM. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-ε -viniferin (3), (+)-ampelopsin F (7), and piceatannol(9) exhibited no activity on three cancer cell lines.

Identification of Soluble Epoxide Hydrolase Inhibitors from the Seeds of Passiflora edulis Cultivated in Vietnam

To Dao Cuong,Hoang Thi Ngoc Anh,Tran Thu Huong,Pham Ngoc Khanh,Vu Thi Ha,Tran Manh Hung,김영호,Nguyen Manh Cuong 한국생약학회 2019 Natural Product Sciences Vol.25 No.4

Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC50 value of 3.4 mM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.

Determinants Influencing the Conversion of Financial Statements: The Case of Multinational Firms in Vietnam

Manh Dung TRAN,Thi Tuyet Mai NGO,To Uyen PHAN,Duc Tai DO,Thi Thuy Hang PHAM 한국유통과학회 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.3

The research aims to investigate the impact levels of determinants on the conversion of financial statements from Vietnamese accounting standards (VAS) to International financial reporting standards (IFRS) in the multinational firms in Vietnam. The method of data collection was done through the survey and subjects are accountants in Multinational Firms doing business in Hanoi and ten neighboring provinces. After checking the information on the votes, there are 170 questionnaires with full information for data entry and analysis. We use Cronbach‟s Alpha, EFA analysis and run regression model to investigate the impact levels of each independent variable on dependent variable of the conversion of VAS financial statements to IFRS. The results show that five determinants including Economic, Politics, Law, Culture, and Conditions for implementation have positive relationships with the conversion of VAS financial statements to IFRS. In particular, Economic determinant is the most strongest. Based on the findings, some recommendations are given for improving the conversion of VAS financial statements to IFRS of multinational firms doing business in the context of Vietnam. The results are considered a useful reference for firms when making financial statements to transparently change the financial statement information and improve the quality of financial statement information.

Protective effects of extract of <i>Cleistocalyx operculatus</i> flower buds and its isolated major constituent against LPS-induced endotoxic shock by activating the Nrf2/HO-1 pathway

Tran, Phi-Long,Kim, Okwha,Tran, Huynh Nguyen Khanh,Tran, Manh Hung,Min, Byung-Sun,Hwangbo, Cheol,Lee, Jeong-Hyung Elsevier 2019 Food and chemical toxicology Vol.129 No.-

<P><B>Abstract</B></P> <P>The flower buds of <I>Cleistocalyx operculatus</I> are used as an important ingredient in herbal tea and herbal products in several tropical countries. However, their protective effects and underlying mechanisms on lipopolysaccharide (LPS)-induced endotoxic shock remain unclear. The aim of this study was to investigate the anti-inflammatory effects of ethanol extract of <I>C. operculatus</I> flower buds (ECO) and its major constituent 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) in macrophages and in an experimental LPS-induced sepsis mouse model. ECO inhibited the LPS-induced production and expression of pro-inflammatory mediators in macrophages. In an endotoxic shock mouse model, the oral administration of ECO rescued LPS-induced mortality, and attenuated LPS-induced increases in the serum levels of pro-inflammatory mediators, and damage of the lung and liver tissues. ECO increased the nuclear translocation of the nuclear factor erythroid 2-related factor 2 (Nrf2), as well as the expression of Nrf2 target genes, including heme oxygenase-1 (HO-1), in macrophages. Similar to the effects of ECO, DMC also inhibited the LPS-induced inflammatory response in macrophages and endotoxic shock in mice, and activated the Nrf2/HO-1 pathway. In conclusion, our findings suggested that ECO and its major constituent, DMC, attenuated LPS-induced endotoxic shock by activating the Nrf2/HO-1 pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The flower buds of Cleistocalyx operculatus are used as an ingredient in herbal products in several tropical countries. </LI> <LI> The anti-inflammatory effects of C. operculatus flower buds (ECO) and its major constituent DMC are demonstrated. </LI> <LI> ECO and DMC attenuates lipopolysaccharide-induced inflammatory response in macrophages and endotoxic shock in mice. </LI> <LI> The Nrf2/HO-1 pathway is attributable to the anti-inflammatory properties of ECO and DMC in macrophages. </LI> <LI> The mechanism explains the anti-inflammatory effects of ECO and DMC. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

iGuardian: Drunk Detection System Based on Wearable Device

Manh-Hung Tran,Vu Nguyen Phan,Sanggil Yeoum(염상길),Hyunseung Choo(추현승) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.6

iCaMs: 안티 콜 피싱 및 메시지 사기를 위한 지능형 시스템

( Manh-hung Tran ),양희규,( Thien-binh Dang ),추현승 ( Hyun-seung Choo ) 한국정보처리학회 2019 한국정보처리학회 학술대회논문집 Vol.26 No.2

The damage from voice phishing reaches one trillion won in the past 5 years following report of Business Korea on August 28, 2018. Voice phishing and mobile phone scams are recognized as a top concern not only in Korea but also in over the world in recent years. In this paper, we propose an efficient system to identify the caller and alert or prevent of dangerous to users. Our system includes a mobile application and web server using client and server architecture. The main purpose of this system is to automatically display the information of unidentified callers when a user receives a call or message. A mobile application installs on a mobile phone to automatically get the caller phone number and send it to the server through web services to verify. The web server applies a machine learning to a global phone book with Blacklist and Whitelist to verify the phone number getting from the mobile application and returns the result.

Lipoxygenase Inhibitory Constituents from Rhubarb

Tran Minh Ngoc,Pham Thi Hong Minh,Tran Manh Hung,IkSoo Lee,민병선,Phuong Thien Thuong,배기환 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5

Phytochemical study on the ethanol extract of rhubarb led to the isolation of fifteen compounds, including five anthraquinones: chrysophanol (1), physcion (2), emodin (7), chrysophanol- 8-O-β-D-glucopyranoside (9) and emodin-8-O-β-D-glucopyranoside (15), and ten stilbenes: desoxyrhaponticin (3), rhaponticin (4), resveratrol (5), desoxyrhapotigenin (6), rhapontigenin (8), piceatannol-3'-O-β-D-glucopyranoside (10), piceid (11), ε -viniferin (12), ampelopsin B (13) and isorhaponticin (14). Their structures were identified by comparing the physicochemical data with those of published papers. Among the isolated compounds, stilbene derivatives (3-6, 8 and 10-14) showed remarkable inhibitory effect on lipoxygenase with IC50 values ranging from 6.7 to 74.1 μM. The inhibition kinetics analyzed by Lineweaver-Burk plots found that they were competitive inhibitors with the linoleic acid at the active site of lipoxygenase. In addition, stilbenes exhibited significantly free radical scavenging activity against ABTS•+ with trolox equivalent activity capacity (TEAC) values ranging from 1.16 to 4.64. Whereas, anthraquinone derivatives (1-2, 7, 9 and 15) neither inhibited lipoxygenase nor scavenged free radical ABTS•+. These results indicated that stilbene derivatives were considerate to be mainly lipoxygenase inhibitor and free radical scavenger constituents of rhubarb.

Acetylcholinesterase Inhibitory Effect of Lignans Isolated from Schizandra chinensis

Tran Manh Hung,나민균,민병선,Tran Minh Ngoc,IkSoo Lee,XinFeng Zhang,배기환 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.6

The hexane extract of the fruit of Schizandra chinensis (Schisandraceae) was found to show significant inhibition of the activity of acetylcholinesterase enzyme (AChE). In further studies, fourteen lignans were isolated, and evaluated for their inhibitory effect on AChE. The compounds having both aromatic methylenedioxy and hydroxyl groups on their cyclooctadiene ring, such as gomisin C (6), gomisin G (7), gomisin D (8), schisandrol B (11) and gomisin A (13), entirely inhibited AChE in dose dependent manners, with IC50 values of 6.71 ± 0.53, 6.55 ± 0.31, 7.84 ± 0.62, 12.57 ± 1.07 and 13.28 ± 1.68 µM, respectively. These results indicate that the lignans could potentially be a potent class of AChE inhibitors.

Cytotoxic Saponins from the Root of Dipsacus asper Wall

Tran Manh Hung,WenYi Jin,Phuong Thien Thuong,송경식,성연희,배기환 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.9

Cytotoxic activitiy of seven hederagenin saponins isolated from the root of Dipsacus asper were investigated in vitro against L1210, HL-60 and SK-OV-3 tumor cell lines by the MTT method. 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin (2), 3-O-β-Dxylopyranosyl-( 1→3)-α-L-Rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin (6) and 3-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl hederagenin (7) exhibited the potent cytotoxicity against the three tumor cell lines with IC50 values ranging from 4.7 to 8.7 µg/mL, with the exception of compound 7, which exhibited weak cytotoxic activity against SK-OV-3 (IC50 22.5 µg/mL). Other compounds did not exhibit any cytotoxic activity (IC50 > 30 µg/mL).

Two Lignans from the Stem Bark of Styrax japonica

Hung, Tran Manh,Lee, Mi-Kyoung,Min, Byung-Sun,Kim, Jin-Cheol,Choi, Jae-Sue,Lee, Hyeong-Kyu The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5

Two lignans, 1R,2R,5S,6R-2-(4'-hydroxy-3'-methoxyphenyl)-6-(3'',4''-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane 4'-O-${\beta}$-D-glucopyranoside (1) and styraxlignolides F (2), were isolated from the EtOAc-soluble fraction of the stem bark of Styrax japonica (Styracaceae). The structures of lignans (1-2) were determined by spectroscopic means. The absolute configuration for styraxlignolides F (2), (2S,3S)-2-(3'',4''-dimethoxybenzyl)-3-(4'-hydroxy-3'-methoxybenzyl)-${\gamma}$-butyrolactone 4'-O-(${\beta}$-D-glucopyranoside), was unambiguously confirmed by X-ray diffraction analysis.