An improvement of real-time polymerase chain reaction system based on probe modification is required for accurate detection of African swine fever virus in clinical samples in Vietnam

Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10

Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.

ĐÁNH GIÁ KHẢ NĂNG LÀM LÀNH VẾT BỎNG TRÊN CHUỘT CỦA HYDROGEL TỪ AgNP-CHITOSAN-CURCUMIN

Hoang Ngoc Anh Nhan,Nguyen Ba Trung,Giang Thi Kim Lien,Truong Thi My Phuong,Ho Kha Vinh Nhan,Pham Xuan Anh 한국베트남학회 2023 베트남연구 Vol.21 No.1

Skin burn is one of the major causes of morbidity and mortality in burn patients due to its susceptibility to infection. The disruption of the epidermal barrier, combined with the denaturation of proteins and lipids, provides a fertile environment that is rich in bacterial nutrients for microbial growth, making it significantly prone to infection. This study aimed to formulate a thermoresponsive hydrogel containing silver nanoparticles (300 ppm), oligo chitosan 2%, and curcumin (0.1%) in the polymer pluronic F127 matrix with a final concentration of 13% to effectively promote the healing of skin wounds. The prepared thermoresponsive hydrogel was investigated for its physical and chemical stability, gelation temperature, and chemical composition. In addition to in vitro antibacterial activity against Staphylococcus aureus found in burn infections, in vivo burn healing and antibacterial activities were also investigated and compared with those of a commercial product using burn-induced infected wounds in mice. The formulation showed antibacterial activity with effective values for wound healing properties, as shown in vivo and by histopathological studies. This study also demonstrates that the thermoresponsive hydrogel was successful as an antibacterial and burned wound-healing transdermal drug delivery system.

Synthesis, characterization, and photocatalytic activity of multicomponent CdMoO₄/g-C₃N₄/GO composite

Hoang Van Duc,Nguyen Thi Anh Thu,Do Thi Ngoc Cam,Nguyen Dinh Luyen,Nguyen Le My Linh,Nguyen Thi Ai Nhung,Dao Ngoc Nhiem,Dinh Quang Khieu 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.6

Inverse giant magnetoresistance due to spin-dependent bulk scattering in Fe_1–xCr_x/Cu/Co

Thi, Ngoc Anh Nguyen,Thi, Hoang Yen Nguyen,Yi, Hyunjung,Joo, Sung-Jung,Shin, Kyung-Ho WILEY-VCH Verlag 2007 Physica status solidi. PSS. A, Applications and ma Vol.204 No.12

<P>Inverse giant magnetoresistance (IGMR) can be observed in multilayers with alternating two ferromagnetic layers F1 and F2 possessing opposite spin scattering asymmetries. We report here the observation of inverse current-in-plane giant magnetoresistance (CIP-IGMR) in Fe<SUB>1–x</SUB>Cr<SUB>x</SUB>/Cu/Co spin-valve systems with varying Cr concentration and FeCr-layer thickness. The highest magnitude of IGMR, –0.45%, has been achieved in the sample doped with 35 at.% Cr. It is shown that the proper substitution of Cr for Fe can alter spin-dependent scattering in the Fe layer, causing the inversion of the bulk spin scattering asymmetry coefficient therefore resulting in CIP-IGMR. The GMR has a dominant contribution to the observed inverse MR, rather than the anisotropic magnetoresistance (AMR) component. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</P>

An overview bioactive compounds on the skin of frogs (Anura)

Tran Thi Huyen,Phan Thi Hoang Anh,Nguyen Thi Anh Hong,Nguyen Ngoc Duyen,Le Pham Tan Quoc,Tran Dinh Thang 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.4

The robust development of frog farming offered high economic benefits but created a large waste residue of frog bones and skin that received little attention. Over the years, inedible by-products have often been processed into biomolecules of potential value and environmental benefits, such as collagen, gelatin, and bioactive peptides. An overview of bioactive compounds on frog skins from various countries indicated that brevinin was the most abundant biological peptide found in frog skin. Other remaining compounds also possessed their highlighted activities, including antibacterial, stimulating insulin release and gastric hormone release, anti-cancer, and neuroregulatory. Notably, various components have been analyzed in the structure and sequence to give meaningful insight into clustering components related to their biological activity. This review may create a source of raw materials for the developmental research of by-products from frog skin and concomitantly reduce environmental pollution.

Tobacco Access and Availability for Vietnamese School Children (aged 13-15): Results from the Global Youth Tobacco Use Survey (GYTS) 2014 in Viet Nam

Le, Thi Kim Anh,Bui, Thi Tu Quyen,Hoang, Van Minh,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

Efforts to reduce tobacco use among school children need to be based on understanding of access to cigarettes by these subjects because previous studies indicated that enforcement of laws for controlling tobacco sales seems to not affect teen/school children because they can obtain cigarettes from different sources. This paper aims to describe access to and availability of cigarettes among school students (aged 13-15 years old) according to the data from GYTS Vietnam 2014. In GYTS, a national school-based survey of students of grades 8-10, our findings showed that about 15% school children are current smokers who smoke at home, and that they could easily buy cigarettes from stores (63.2%), or someone else (27.8%), or street vendors (9%). Notably, over 85% of school children answered that they were not refused because of their age. This high percentage was nearly the same in the North (85.7%), the Centre (92.5%), and the South (89.7%) of Viet Nam. These findings show that it is quite easy for school children to obtain cigarettes and this is a crucial challenge for policy makers aiming to reduce tobacco use among youth in general and school-age students in particular.

Uptake of arsenic and heavy metals by native plants growing near Nui Phao multi-metal mine, northern Vietnam

Ha, Nguyen Thi Hoang,Ha, Nguyen Thi,Nga, Tran Thi Huyen,Minh, Nguyen Ngoc,Anh, Bui Thi Kim,Hang, Nguyen Thi An,Duc, Nguyen Anh,Nhuan, Mai Trong,Kim, Kyoung-Woong Elsevier 2019 Applied geochemistry Vol.108 No.-

<P><B>Abstract</B></P> <P>Phytoremediation is a plant-based, environment-friendly, and cost-effective technology that can be potentially used to remediate contaminated media. This study was conducted to evaluate the phytoextraction and phytostabilization potential, two common techniques of phytoremediation, of 21 plant species growing naturally at the largest tungsten (W) mine in Vietnam—the second largest production of W in the world. The average concentrations in different soil fractions were in the following order: residue > organically complexed, Fe- and Mn oxide-bound > carbonate bound > exchangeable. The total concentrations of arsenic (As), cadmium (Cd), copper (Cu), and zinc (Zn) in the soil varied by 34–3390, 4.87–81.6, 14.3–2080, and 21.9–370 mg/kg, respectively. The concentrations of As, Cd, Cu, and Zn in the shoots ranged 0.71–2400, 0.05–5.55, 4.81–249, and 13.3–380 mg/kg-DW, respectively. The concentrations of As and Cd in all soil samples and Cu in 70% of soil samples collected around the mine fluctuated within 2–227, 3–54, and up to 21-folds higher than the maximum allowable limit for agricultural soils in Vietnam (QCVN 03-MT:2015/BTNMT). The results indicated that hyperaccumulation levels (mg/kg-DW) were obtained for only As in <I>Pityrogramma calomelanos</I> (2400) and <I>Pteris vittata</I> L. (1860). Based on the recorded hyperaccumulation levels, translocation and accumulation factors, biomass, and fast growth of these plants, <I>P. calomelanos</I> and <I>P. vittata</I> were considered to be promising native plants for the phytoextraction of As contaminated soils. <I>Bidens pilosa</I> L. also has great potential for phytostabilization of mining soils contaminated with As and heavy metals.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soils near multi-metal (W–F–Cu–Au–Bi) mine were contaminated with As, Cd, Cu. </LI> <LI> Arsenic in shoots were in order of Fern > Monocotyledonae > Dicotyledonae. </LI> <LI> <I>Pityrogramma calomelanos</I> and <I>Pteris vittata</I> L. suggested for phytoextraction of As. </LI> <LI> <I>Bidens pilosa</I> L. suggested for the phytostabilization of mining contaminated soils. </LI> </UL> </P>

A novel mathematical modelling for simulating the spread of heavy metals in solid waste landfills

Ngoc Ha Hoang,Anh My Chu,Kim Thai Thi Nguyen,Chi Hieu Le 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3

Municipal solid waste (MSW) landfills create the environmental pollutions due to problems related to toxins, leachate, and greenhouse gas, which are the growing environmental concerns, especially in developing countries. This paper presents a novel mathematical modelling for simulating the spread of heavy metals in soil layers in the MSW landfills. A new mathematical modelling procedure is studied for deriving the governing equation which describes the time varying concentration of pollutants in the leachate in a 3-dimensional (3D) space of soil layers. A Finite Element Method (FEM) based algorithm is then constructed to solve numerically the governing equation over time in 3D space for effectively analyzing the variation of pollutants concentrations. Finally, the applicability and advantages of the proposed novel method are demonstrated through the case study with soil samples collected from the actual MSW landfill. The successfully developed algorithm can be applied to model and predict the spread of different heavy metals in the MSW landfill sites; and to develop effective solutions and policies for solid waste classification and management, in order to minimise the negative impacts from the potential risk of the toxic heavy metal pollutions, soil contaminations and groundwater pollutions in the areas nearby the MSW landfill.

Factors Affecting the Internal Control System: A Case Study of Chemical Enterprises in Vietnam

Hoang Thanh NGO,Tung Dao NGUYEN,Nga Thuy Thi NGUYEN,Ha Ngoc DAO,Anh Phuong Tran VU 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.12

The article analyzes the impact of factors affecting the internal control system of chemical enterprises in Vietnam, providing more empirical evidence on factors affecting the system of internal control. Research data is collected in the form of face-to-face interviews and email interviews with managers and employees working in different positions, managers at all levels, chief accountants, and accountants of 52 chemical enterprises in Vietnam. The survey results collected 310 questionnaires. After eliminating the invalid questionnaires due to many blank cells, the authors chose to use 297 questionnaires. Quantitative research was carried out with SPSS 25 software. Research results show that Business Strategy, Organizational Structure, Organization’s perception of the instability of the external environment, and Organizational culture are the factors that positively affect the internal control system of chemical enterprises. This can be explained that, for chemical enterprises, Business strategy, Organizational structure, Organization’s perception of the instability of the external environment, and Good organizational culture will positively impact the internal control system of chemical enterprises. Based on the research results, the authors have proposed recommendations to enhance the effectiveness of the internal control system of chemical enterprises in Vietnam, thereby contributing to improving the performance of enterprises in the chemical Vietnamese.

Tobacco Control Policies in Vietnam: Review on MPOWER Implementation Progress and Challenges

Hoang, Van Minh,Tran, Thu Ngan,Vu, Quynh Mai,Nguyen, Thi Tuyet My,Le, Hong Chung,Vu, Duy Kien,Tran, Tuan Anh,Nguyen, Bao Ngoc,Vu, Van Giap,Nguyen, Manh Cuong,Pham, Duc Manh,Kim, Bao Giang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1

In Vietnam, the WHO Framework Convention on Tobacco Control (WHO FCTC) took effect in March 2005 while MPOWER has been implemented since 2008. This paper describes the progress and challenges of implementation of the MPOWER package in Vietnam. We can report that, in term of monitoring, Vietnam is very active in the Global Tobacco Surveillance System, completing two rounds of the Global Adult Tobacco Survey (GATS) and three rounds of the Global Youth Tobacco Survey (GYTS). To protect people from tobacco smoke, Vietnam has issued and enforced a law requiring comprehensive smoking bans at workplaces and public places since 2013. Tobacco advertising and promotion are also prohibited with the exception of points of sale displays of tobacco products. Violations come in the form of promotion girls, corporate social responsibility activities from tobacco manufacturers and packages displayed by retail vendors. Vietnam is one of the 77 countries that require pictorial health warnings to be printed on cigarette packages to warn about the danger of tobacco and the warnings have been implemented effectively. Cigarette tax is 70% of factory price which is equal to less than 45% of retail price and much lower than the recommendation of WHO. However, Vietnam is one of the very few countries that require manufacturers and importers to make "compulsory contributions" at 1-2% of the factory price of cigarettes sold in Vietnam for the establishment of a Tobacco Control Fund (TCF). The TCF is being operated well. In 2015, 67 units of 63 provinces/cities, 22 ministries and political-social organizations and 6 hospitals received funding from TCF to implement a wide range of tobacco control activities. Cessation services have been starting with a a toll-free quit-line but need to be further strengthened. In conclusion, Vietnam has constantly put efforts into the tobacco control field with high commitment from the government, scientists and activists. Though several remarkable achievements have been gained, many challenges remain. To overcome those challenges, implementation strategies that take into account the contextual factors and social determinants of tobacco use in Vietnam are needed.