Furosemide에 의한 소디움 운반체 발현의 변화

오윤규 ( Yoon Kyu Oh ),나기영 ( Ki Young Na ),이재욱 ( Jay Wook Lee ),장혜련 ( Hye Ryun Chang ),박영선 ( Young Sun Park ),박정환 ( Jung Hwan Park ),주권욱 ( Kwon Wook Joo ),김근호 ( Gheun Ho Kim ),이정상 ( Jung Sang Lee ),한진석 ( 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.2

배경 : 임상에서 흔히 사용하는 이뇨제 furosemide는 비후상행각에서 Na+-K+-2CI- cotranspoter (NKCC2)를 억제하여 NaCl 재흡수를 차단하여 이뇨작용을 나타낸다. Furosemide를 장기간 투여하면 내성과 대사성 알카리증의 부작용이 발생할 수 있는데, 이는 집합관에 도달하는 소디움 증가와 관련 있을 가능성이 있다. 방법 : 저자들은 furosemide의 내성이나 부작용이 집합관 상피 소디움 통로 (ENaC) 단백발현의 변화와 관련이 있는지를 확인하고자, Sprague-Dawley rat에서 farosemide (12 mg/day)을 7일간 지속적 피하 주입한 후 반정량적 immunoblotting과 면역조직화학법을 이용하여 NKCC2, Na +-CL- cotransporter (NCC), ENaC 단백의 발현을 관찰하였다. 실험기간 동안 수분과 전해질 용액 (0.8% NaCl & 0.1% KCl)을 자유롭게 섭취하도록 하여 체액 감소를 방지하였다. 결과 : 부형약 (vehicle)을 투여한 대조군에 비하여, furosemide를 투여한 군에서 각각 요량과 요 소디움 배설이 증가하였으나, 체중, 혈청 알도스테론치 및 크레아티닌 청소율은 차이가 없었다. Furosemid 투여 후 NKCC2 단백은 피질 (151±10 vs. 100±10%, p<0.05)과 외수질 (122±5 vs. 100±3%, p<0.01)에서 증가해 있었다. ENaC 단백은 세 가지 subunit 모두 furosemide 투여 후 대조군에 비하여 피질 (α:187±25 vs. 100±22%, p<0.05; β:155±8 vs. 100±15%, p<0.05; γ:168±16 vs. 100±9%, p<0.05)과 외수질 (α:171±27 vs. 100±17%, p<0.05; β :986±91 vs. 100±33%, p<0.01; γ :242±24 vs. 100±22%, p<0.01)에서 증 가하였다. 면역조직화학법에서도 furosemide를 투여한 군의 집합관 주세포에서 ENaC β-subunit가 더 강하게 염색되었다. 결론 : 이상에서 장기간 furosemide 투여시 집합관 ENaC 발현이 증가하며, 이러한 원위부네프론의 적응 반응이 이뇨제 내성을 유발하는데 기여할 것으로 생각한다. Background : Furosemide inhibit NaCl absorption in the thick ascending limb and produce an increase in distal delivery of Na+. We carried out semiquantitative immunoblotting and immunohistochemistry of rat kidneys to investigate whether chronic furosemide infusion is associated with compensatory increases in the abundance of Na+ transporters in distal nephron. Methods : Osmotic minipumps were implanted into Sprague-Dawley rats to deliver 12 mg/day of furosemide(n=6) with simultaneous administration of 0.8% NaCl and 0.1% KCl in drinking water for 7days. Results : Compared with vehicle infused controls, urine volume and urine sodium amount were increased. However, there were no differences in body weight, serum aldosterone, and creatinine clearance. The abundance of Na+-K+-2CI - cotransporter after furosemide infusion was increased in cortex (151±10 vs. 100±10%, p<0.05) and outer medulla (122±5 vs. 100±3%, p<0.01). In furosemide infusion group, the abundance of all three subunits of epithelial sodium channel (ENaC) was increased both in cortex (α:187±25 vs. 100±17%, p<0.05; β:155±8 vs. 100±15%, p<0.05; γ :168±16 vs. 100±9%, p<0.05) and outer medulla (α:171±27 vs. 100±17%, p<0.05; β :986±91 vs. 100±33%, p<0.01; γ :242±24 vs. 100±22%, p<0.01). Consistent with these results, ENaC β-subunit immunohistochemistry showed a remarkable increase in immunoreactivity in the principal cells of collecting ducts with furosemide treatment. Conclusion : These increases in the abundance of ENaC protein may account for the generation of diuretic tolerance.

Hierarchically nanoporous pyropolymer nanofibers for surface-induced sodium-ion storage

Yoon, Hyeon Ji,Lee, Min Eui,Kim, Na Rae,Yang, Seung Jae,Jin, Hyoung-Joon,Yun, Young Soo Pergamon Press 2017 Electrochimica Acta Vol. No.

<P><B>Abstract</B></P> <P>Surface-driven charge storage materials based on both electrochemical double layer (EDL) formation and pseudocapacitive behavior can deliver high energy and power capabilities with long-lasting cycling performance. On the other hand, the electrochemical performance is strongly dependent on the material properties, requiring sophisticated electrode design with a high active surface area and a large number of redox-active sites. In this study, hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH. The HN-PNFs have a hierarchically nanoporous structure and an exceptionally high specific surface area of 3,950.7m<SUP>2</SUP> g<SUP>−1</SUP> as well as numerous redox-active heteroatoms (C/O and C/N ratio of 10.6 and 16.8, respectively). These unique material properties of HN-PNFs resulted in high reversible Na-ion capacity of ∼292mAhg<SUP>−1</SUP> as well as rapid kinetics and stable cycling performance in the cathodic potential range (1-4.5V vs. Na<SUP>+</SUP>/Na). Furthermore, energy storage devices based on HN-PNFs showed a remarkably high specific energy of ∼258 Wh kg<SUP>−1</SUP> at ∼245Wkg<SUP>−1</SUP> as well as a high specific power of ∼21,500Wkg<SUP>−1</SUP> at ∼78 Wh kg<SUP>−1</SUP>, with long and stable cycling behaviors over 2,000 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) were fabricated. </LI> <LI> HN-PNFs have a high specific surface area of ∼3,950m<SUP>2</SUP> g<SUP>−1</SUP> and numerous heteroatoms. </LI> <LI> HN-PNFs exhibit high capacities of ∼292mAhg<SUP>−1</SUP> and great rate/cycling performances. </LI> <LI> High energy (258Whkg<SUP>−1</SUP>) and high power (21,500Wkg<SUP>−1</SUP>) are achieved. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Hierarchically nanoporous pyropolymer nanofibers (HN-PNFs) fabricated from electrospun polyacrylonitrile nanofibers by simple heating with KOH exhibited high electrochemical performance for Na-ion storage.</P> <P>[DISPLAY OMISSION]</P>

A Na⁺-coupled C₄-dicarboxylate transporter (Asuc_0304) and aerobic growth of <i>Actinobacillus succinogenes</i> on C₄-dicarboxylates

Rhie, Mi Na,Yoon, Hyo Eun,Oh, Hye Yun,Zedler, Sandra,Unden, Gottfried,Kim, Ok Bin Society for General Microbiology 2014 Microbiology Vol.160 No.7

<P><I>Actinobacillus succinogenes</I>, which is known to produce large amounts of succinate during fermentation of hexoses, was able to grow on C<SUB>4</SUB>-dicarboxylates such as fumarate under aerobic and anaerobic conditions. Anaerobic growth on fumarate was stimulated by glycerol and the major product was succinate, indicating the involvement of fumarate respiration similar to succinate production from glucose. The aerobic growth on C<SUB>4</SUB>-dicarboxylates and the transport proteins involved were studied. Fumarate was oxidized to acetate. The genome of <I>A. succinogenes</I> encodes six proteins with similarity to secondary C<SUB>4</SUB>-dicarboxylate transporters, including transporters of the Dcu (C<SUB>4</SUB>-dicarboxylate uptake), DcuC (C<SUB>4</SUB>-dicarboxylate uptake C), DASS (divalent anion : sodium symporter) and TDT (tellurite resistance dicarboxylate transporter) family. From the cloned genes, Asuc_0304 of the DASS family protein was able to restore aerobic growth on C<SUB>4</SUB>-dicarboxylates in a C<SUB>4</SUB>-dicarboxylate-transport-negative <I>Escherichia coli</I> strain. The strain regained succinate or fumarate uptake, which was dependent on the electrochemical proton potential and the presence of Na<SUP>+</SUP>. The transport had an optimum pH ~7, indicating transport of the dianionic C<SUB>4</SUB>-dicarboxylates. Transport competition experiments suggested substrate specificity for fumarate and succinate. The transport characteristics for C<SUB>4</SUB>-dicarboxylate uptake by cells of aerobically grown <I>A. succinogenes</I> were similar to those of Asuc_0304 expressed in <I>E. coli</I>, suggesting that Asuc_0304 has an important role in aerobic fumarate uptake in <I>A. succinogenes</I>. Asuc_0304 has sequence similarity to bacterial Na<SUP>+</SUP>-dicarboxylate cotransporters and contains the carboxylate-binding signature. Asuc_0304 was named SdcA (<U>s</U>odium-coupled C<SUB>4</SUB>-<U>d</U>i<U>c</U>arboxylate transporter from <I><U>A</U></I>. <I>succinogenes</I>).</P>

이뇨제의 저항성과 내성 기전

오윤규,나기영,김근호,김소영,한진석 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

콘크리트 내구성 통합 실험 기법 Yoon’s Test의 개발

윤인석 ( Yoon In-seok ),우영웅 ( Woo Young-woong ),김나리 ( Kim Na-ri ),맹찬호 ( Mang Chan-ho ) 한국구조물진단유지관리공학회 2013 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.17 No.2

Three-Dimensional Z-Plasty(Yoon's Method)를 이용한 내안각 췌피 성형술

박흥식,김우신,윤진호,나민화,김한중 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

The presence of epicanthal folds in Asian eyelids is one of their unique features, in combination with the lack of supratarsal folds. Western culture has influenced many people to prefer to eliminate the prominent epicanthal fold. A number of surgical techniques have been suggested for their correction. However, difficulty with design, excessive and prominent scarring of medial canthal and nasal area, recurrence, and rigidity of application are potential problems associated with many procedures. This paper describes a epicanthoplasty with three-dimensional Z-plasty. Through epicanthal fold incision, the medial canthal tendon is medially advanced and sutured to the periosteum of nasal bone. After the transposition of the flaps, trimming of the flap is usually required. Three-dimensional Z-plasty creates the attractive eyes. From March, 1995 to March, 1997, the technique was applied to 37 patients with epicanthal fold and was performed with or without double-fold operation. There were 34 females and 3 males with ages ranging from 17 to 30 years. Through 2 years follow-up, this technique has delivered esthetically good results with minimal postoperative scar and could made the reduction of ICD from 40 ± 2.13 mm to 34 ± 1.98 mm. The advantages of epicanthoplasty procedure using three-dimensional Z-plasty(Yoon's method) are as follows; 1) simple in design 2)minimal postoperative scar in the medial canthal area 3) versatile in its application 4) no recurrence 5) no hypertrophic scar 6) preserving ethnic identity.

무전해 Co-W-P 도금층의 자기적 특성

김창욱,윤성렬,한승희,나재훈,이창재 광운대학교 신기술연구소 1998 신기술연구소논문집 Vol.27 No.-

합금박막형 자기기록매체의 제작방법은 스퍼터링과 무전해 도금방법이 주로 이용되고 있으며, 미국이나 일본등에서는 스퍼터링 방법에 비하여 대량생산이 용이하고. 도금조건에 따라 다양한 특성의 합금박막을 제조할 수있는 무전해 도금방법을 이용한 합금자성박막에 대하여 많은 연구를 하고 있지만 국내에서는 이에 관한 연구가매우 미약한 실정이다. 따라서 이 연구에서는 차아인산이수소나트륨을 환원제로 사용한 무전해 도금법을 이용하여 corning glass 2948 유리기판 위에 Co-W-P 도금충을 제조할 때, pH 및 온도에 따른 석출속도, 합금조성 및 미세구조와 자기적 특성을 고찰하였다 무전해 Co-W-P 도금충의 경우는 자기적 특성에서 보자력은 5000e, 각형비는 0.6 정도의 경향을 보였지만, 결정배향에 있어서는 α-Co (0002) 방향으로 우선 배향하여 수직자화벡터를 형성함을 확인할 수 있었으며, 합금조성에 있어 인(P)의 함량은 0.8±0.2%로 일정하였고, W의 석출량은 Na_2W0_4의 농도가 증가할수록 증가하여 0.1mol/L일 때 20%이였다 수소가스를 이용한 환원분위기에서100℃간격으로 1시간씩 400℃까지 열처리에 따른 자기적 특성과 미세구조의 변화를 확인하여 본 결과Co-W-P 는 열처리에 따라 표면의 평활도는 향상되었지만, 자기적 특성과 미세구조에는 아무런 변화가 없었다. Usually sputtering and electroless plating methods were used for manufacturing metal-alloy thin film magnetic memory devices. Since electroless plating method has many merits in mass production and product variety compared to sputtering method, many researches about electroless plating have been Performed in the United State of America and Japan. However, electroless plating method has not much been studied in Korea. In these respects the purpose of this research is manufacturing Co-W-P alloy thin film on the corning glass 2948 by electroless plating method using sodium hypophosphite as a reductant, and analyzing deposition rate. alloy composition. microstructure, and magnetic characteristics at various pH's and temperatures. For Co-W-P alloy thin film. coercive force was 5000e and squareness was 0.6. For crystal orientation, (0002) orientation of α-Co was dominatly found. Then we could confirm the formation of perpendicular magnetization. The content of P was constant at 0.8±0.2% and the content of W increased as the concentration of Na_2W0_4 increased. When the concentration ofNa_2W0_4 was 0.1 mol/L. the composition of W was 20%. We observed the changes of magnetic characteristics and microstructure of thin film depositions of Co-W-P by the heat treatment. For heat treatment, the temperature was increased step by step to l00℃, 200℃. 300℃, and 400℃.and it took 1 hour at each step in the reductive condition of hydrogen gas. By the heat treatment. flatness of surface was improved, but there were no changes on the magnetic characteristics and the microstructures.

Gintonin facilitates catecholamine secretion from the perfused adrenal medulla

Seung-Yeol Na,Ki-Hwan Kim,Mi-Sung Choi,Kang-Su Ha,Dong-Yoon Lim 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.6

The present study was designed to investigate the characteristics of gintonin, one of components isolated from Korean Ginseng on secretion of catecholamines (CA) from the isolated perfused model of rat adrenal gland and to clarify its mechanism of action. Gintonin (1 to 30 μg/ml), perfused into an adrenal vein, markedly increased the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. The gintonin-evoked CA secretion was greatly inhibited in the presence of chlorisondamine (1 μM, an autonomic ganglionic bloker), pirenzepine (2 μM, a muscarinic M₁ receptor antagonist), Ki14625 (10 μM, an LPA_1/3 receptor antagonist), amiloride (1 mM, an inhibitor of Na⁺/Ca²⁺ exchanger), a nicardipine (1 μM, a voltage-dependent Ca²⁺ channel blocker), TMB-8 (1 μM, an intracellular Ca²⁺antagonist), and perfusion of Ca²⁺-free Krebs solution with 5mM EGTA (a Ca²⁺chelater), while was not affected by sodium nitroprusside (100 μM, a nitrosovasodialtor). Interestingly, LPA (0.3~3 μM, an LPA receptor agonist) also dose-dependently enhanced the CA secretion from the adrenal medulla, but this facilitatory effect of LPA was greatly inhibited in the presence of Ki 14625 (10 μM). Moreover, acetylcholine (AC)-evoked CA secretion was greatly potentiated during the perfusion of gintonin (3 μg/ml). Taken together, these results demonstrate the first evidence that gintonin increases the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. This facilitatory effect of gintonin seems to be associated with activation of LPA- and cholinergic-receptors, which are relevant to the cytoplasmic Ca²⁺ increase by stimulation of the Ca²⁺ influx as well as by the inhibition of Ca²⁺ uptake into the cytoplasmic Ca²⁺ stores, without the increased nitric oxide (NO). Based on these results, it is thought that gintonin, one of ginseng components, can elevate the CA secretion from adrenal medulla by regulating the Ca²⁺ mobilization for exocytosis, suggesting facilitation of cardiovascular system. Also, these findings show that gintonin might be at least one of ginseng-induced hypertensive components.

Resveratrol Inhibits Nicotinic Stimulation-Evoked Catecholamine Release from the Adrenal Medulla

Seong-Chang Woo,Gwang-Moon Na,Dong-Yoon Lim 대한생리학회-대한약리학회 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.4

Resveratrol has been known to possess various potent cardiovascular effects in animal, but there is little information on its functional effect on the secretion of catecholamines (CA) from the perfused model of the adrenal medulla. Therefore, the aim of the present study was to determine the effect of resveratrol on the CA secretion from the isolated perfused model of the normotensive rat adrenal gland, and to elucidate its mechanism of action. Resveratrol (10∼100ՌM) during perfusion into an adrenal vein for 90 min inhibited the CA secretory responses evoked by ACh (5.32 mM), high K⁺ (a direct membrane-depolarizer, 56 mM), DMPP (a selective neuronal nicotinic N_n receptor agonist, 100ՌM) and McN-A-343 (a selective muscarinic M₁ receptor agonist, 100ՌM) in both a time- and dose- dependent fashion. Also, in the presence of resveratrol (30ՌM), the secretory responses of CA evoked by veratridine 8644 (an activator of voltage-dependent Na⁺ channels, 100ՌM), Bay-K-8644 (a L-type dihydropyridine Ca²⁺ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca²⁺-ATPase inhibitor, 10ՌM) were significantly reduced. In the simultaneous presence of resveratrol (30ՌM) and L-NAME (an inhibitor of NO synthase, 30ՌM), the CA secretory evoked by ACh, high K⁺, DMPP, McN-A-343, Bay-K-8644 and cyclopiazonic acid were recovered to a considerable extent of the corres</SUP>ponding control secretion compared with the inhibitory effect of resveratrol alone. Interestingly, the amount of nitric oxide (NO) released from the adrenal medulla was greatly increased in comparison to its basal release. Taken together, these experimental results demonstrate that resveratrol can inhibit the CA secretory responses evoked by stimulation of cholinergic nicotinic receptors, as well as by direct membrane-depolarization in the isolated perfused model of the rat adrenal gland. It seems that this inhibitory effect of resveratrol is exerted by inhibiting an influx of both ions through Na⁺ and Ca²⁺ channels into the adrenomedullary cells as well as by blocking the release of Ca²⁺ from the cytoplasmic calcium store, which are mediated at least partly by the increased NO production due to the activation of NO synthase.

Gemcitabine 이 봉입되고 CR2945가 결합된 리포좀 제제의 in vivo 항암 활성

윤나영,김진석 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

항암제 gemcitabine 이 봉입되고 CR 2945 를 표적 지향적 리간드로 결합시킨 리포좀을 이용하여 인간 췌장암세포(PANC-I)가 이식된 실험용쥐 (Balb/cnumice) 에 주사하여 그 항암활성을 조사하였다. Gemcitabine 이 봉입되고 CR 2945 가 결합된 리포좀과 CR 2945 가 비결합된 리포좀 군에서는 투여 중간에 약물자체의 독성에 의해 사망한 쥐는 없었으나 gemcitabine을 PBS에 녹여 투여한 군에서는 3회 투여부터 사망한 쥐가 발생하였다. 그러나 항암효과 면에서는 Gemcitabine 이 봉입되고 CR 2945 가 결합된 리포좀군, free gemcitabine 군, 그리고 PBS 군 사이에 통계학적인 차이가 나타나지 않았다. 이는 리포좀에 봉입함으로써 gemcitabine 의 독성은 줄어 들었지만 항암효과는 크게 향상시키지 못했음을 나타내고 있기에 제형 개발에 대한 연구가 더 진행되어야 할 것으로 생각된다.