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사람-사람 하이브리도마의 생산을 위한 fusion partner cell의 개발
안지영,김혜원,서동상 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1
This study was performed to establish human hybridmyeloma cell lines as fusion partner cells for producing human-human hybridoma. Human myeloma, SKO-007(J3), and human cervix epitheloid carcinoma cell line, HeLa MR, were fused by using with 50% PEG, and cultured in RPMI 1640 medium containing 15% FBS. Hybrids of SKO-007(J3) and HeLa MR were selected in RPMI 1640 media containing 6-thioguanine(6-TG ; gradually increasing concentration from 10 ㎍/ml to 20 ㎍/ml, and then gradually decreasing to 10 ㎍/ml) for 6 weeks. 6-TG resistant cells were collected and cloned by limiting dilution method. The fused myelomas with HeLa MR grew more rapidly than the fused cells with a small number of HeLa MR. Their doubling time was 72 hours.
( Dong Hye Suh ),( Jeong Hwee Choi ),( Sang Jun Lee ),( Ki Heon Jeong ),( Kye Yong Song ),( Min Kyung Shin ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: High-intensity focused ultrasound (HIFU) and radiofrequency (RF) are used for non-invasive skin tightening. Neocollagenesis and neoelastogenesis have been reported to have a mechanism of controlled thermal injury. Objectives: To compare neocollagenesis and neoelastogenesis in each layer of the dermis after each session of HIFU and monopolar RF. Methods: We analyzed the area fraction of collagen and elastic fibers using the Masson’s trichrome and Victoria blue special stains, respectively, before and after 2 months of treatments. Histometric analyses were performed in each layer of the dermis, including the papillary dermis, and upper, mid,and deep reticular dermis. Results: Monopolar RF led to neocollagenesis in the papillary dermis, and upper, mid, and deep reticular dermis, and neoelastogenesis in the papillary dermis, and upper and midreticular dermis. HIFU led to neocollagenesis in the mid and deep reticular dermis and neoelastogenesis in the deep reticular dermis. Among these treatment methods, HIFU showed the highest level of neocollagenesis and neoelastogenesis in the deep reticular dermis. Conclusion: HIFU affects deep tissues and impacts focal regions. Monopolar RF also affects deep tissues, but impacts diffuse regions. We believe this data provide further insight into effective skin tightening.
( Dong Hye Suh ),( Hee Kyung Lim ),( Hyun Joo Kim ),( Sang Jun Lee ),( Tae In Kim ),( Ki Heon Jeong ),( Min Kyung Shin ),( Kye Yong Song ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Various treatment modalities have been used to treat acne scars. Carbon dioxide (CO2) gas subcision into the dermis by needle with high pressure can cause breakage of fibrotic collagen taking effect of not only subcision but also CO2 gas effects. CO2 fractional laser is the effective and commonly used tool for acne scar to ablation. Objectives: This study was conducted to evaluate the efficacy and safety of subcision with CO2 gas and CO2 fractional laser. Methods: Fourteen patients with skin phototype III and V with acne scars were treated 3 sessions of CO2 gas subcision with 2-week interval and 2 sessions of fractional laser with 4-week intervals. The clinical improvements were assessed in terms of acne scar using a quartile grading scale. Any adverse effects were assessed during the entire study period. A punch biopsy was performed before and after treatment in 10 patients for histologic analysis. Results: Excellent result show 7%, marked result in 57%, moderate result in 29%, and mild improved show in 7%. Histologic evaluation of skin biopsy samples using hematoxylin and eosin and Victoria blue stains showed greater dermal collagen with thickening of the dermis and straightening of elastic fibers in the reticular dermis after treatment. Conclusion: Combination therapy using subcision CO2 gas and fractional laser with 2 sessions was satisfactory and safe for acne scar.
( Dong Hoon Suh ),( Hye Yon Cho ),( Bang Hyun Lee ),( Ki Dong Kim ),( Jae Hong No ),( Yong Beom Kim ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: To identify major determinants of successful laparoscopic radical hysterectomy (LRH) versus abdominal radical hysterectomy (ARH) in early-stage cervical cancer for inexperienced surgeons (IS). 방법: Consecutive 161 patients with stage IA2-IIA cervical cancer who underwent RH were grouped into two according to the surgeons` experience of LRH: experienced surgeon (ES, 40 LRH versus 57 ARH) versus IS (15 LRH versus 49 ARH). After matching for age and risk factors, surgical and survival outcomes were compared. Short postoperative hospital stay (PHS) was considered as a surrogate marker for fast recovery without serious complications. 결과: Earlier-stage and lesser risk factors of LRH than ARH in ES were not observed in IS. After matching, vaginal tumor-free margin (VFM) of LRH was shorter than that of ARH in ES (1.3 cm versus 1.7 cm, p=0.007), however, longer than that of ARH in IS (1.8 cm versus 1.3cm, p=0.035). PHS of LRH was shorter than that of ARH in ES (5.5days versus 7.7days, p<0.001), however, not different from that of ARH in IS. VFM > 1.8 cm (OR 7.33, 95% CI 1.22-40.42), stage > IB1 (OR 8.83, 95% CI 1.51-51.73), and estimated blood loss > 575ml (OR 33.95, 95% CI 4.87-236.79) were independent risk factors for longer PHS in IS. There was no difference of 5yr-profression-free survival of LRH patients between ES and IS after matching (55.1 % versus 33.3 %, p=0.391). 결론: Selection of earlier-stage disease and moderate VFM might be important for IS to successfully perform LRH with minimal complication in early-stage cervical cancer.
( Dong-hye Suh ),( Haneul Oh ),( Sang Jun Lee ),( Hyun Joo Kim ),( Hwa Jung Ryu ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Acne vulgaris is one of the most common dermatological diseases, and it is known that various factors are related to triggering this condition in a patient. In particular, although the use of cosmetics is thought to be associated with the onset of a patient developing acne, there are few studies that have been conducted to date on this disease and its related factors. Objectives: To investigate the relationship between cosmetic use patterns and acne, in addition to clinical features for acne patients. Methods: To begin with, a total of 539 patients with acne vulgaris, who visited three dermatologic clinics in Korea, were asked to complete a questionnaire regarding their condition in relation to clinical features and exacerbating factors, including the use of cosmetics. Results: Among the respondents, 38.1% of patients who failed treatment answered that the condition of their acne was worsened by the continued use of cosmetics, which was significantly higher than 26.3% of the surveyed treatment-naive patients (p<0.05). In this relation, double cleansing and the number of cosmetics in use were also noted in higher numbers than in the treatment-failure patients. As noted, the liquid-type foundation and concealer were frequently used in this group. Conclusion: This study showed that overall dermatologists should consider advising about cosmetic use to acne patients along with medical treatment options for the patients with acne.
P111 The efficacy and safety of fractional unipolar radiofrequency on facial tightening
( Dong-hye Suh ),( Eun Jung Byun ),( Sang-jun Lee ),( Kye Yong Song ),( Hei Sung Kim ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2
<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: The medical use of radiofrequency (RF) is based on an oscillating electrical current that forces collisions between charged molecules and ions, which are then transformed into heat. Water is the main target for this process. RF-generated tissue heating has different biological and clinical effects, depending on the depth of tissue targeted, the frequency used, and the specific cooling of the dermis and epidermis. RF can be delivered using monopolar, bipolar, and unipolar devices. With unipolar RF, there is one electrode, no grounding pad, and a large field of RF is emitted in an omnidirectional field around a single electrode. A unipolar hand-piece delivers RF energy to an estimated depth of up to 20 mm. Objectives: Herein, we aimed to assess the efficacy and safety of a fractional unipolar RF device on facial tightening. Methods: Fractional unipolar RF (tuneface hand-piece, AccentTM Prime, Alma Lasers, Inc., Fort Lauderdale, FL) was applied to the individual’s face every 2 weeks for a total of 5 sessions. Results: A total of 14 patients (all female, mean age: 49.7 years) were included in this study. The majority of patients (85.7%) answered that the results were good or excellent. Consensus ratings by 2 physicians found that the objective outcomes were divided among “75-100% improvement (35.7%)”, “50-74% improvement (50%)”, “25-49% improvement (14.3%)”, and “improvement of less than 25% (0%)”. Skin biopsies from the face revealed increase of collagen in the dermis. The incidence of complications was low and minor. Conclusion: Unipolar RF device with a novel mobile energy delivery offers an additional step in non-ablative laser resurfacing for rhytides and skin laxity.