초음파 사용이 건강한 여성의 피부 탄력과 피부 깊은 탄력 개선에 미치는 영향

고민주,김기수,장은미,오재섭 대한통합의학회 2023 대한통합의학회지 Vol.11 No.1

Purpose : Aging is reflected on the face of women due to the depletion of collagen and hydration in the facial skin overtime. This study investigated the effect of multiple SONO® ultrasound for a 4-week period on the skin health by measuring the skin elasticity and deeper skin elasticity in the tested women subjects. Methods : Twenty healthy women were recruited for this experiment. All the participants applied multiple ultrasound device (SONO®) during this experiment. The SONO® device was set to ANTI-AGING function and five power steps such as 1, 3, 10 and 17 ㎒ were used during this experiment, and directly contacted with the facial skin. Specifically, the probe was contacted with the entire face except for the nose and eyes for 10 min on each side of the face every day and repeated for 4 weeks. The skin elasticity and the elasticity of deeper skin were measured at three times (0, 2, 4 weeks) using a Ballistometer and dermal torque meter, respectively. The one way repeated ANOVA was used to compare the skin elasticity and the elasticity of deeper skin among three times (0, 2, 4 weeks). Results : The skin elasticity (p<.05) and elasticity of deeper skin (p<.05) were significantly increased at 2 weeks and 4 weeks of intervention compared to that at 0 weeks. For the skin elasticity, there was no significant difference between 2 and 4 weeks of intervention (p>.05). For the elasticity of deeper skin, it increased significantly at 4 weeks compared to 2 weeks of intervention (p<.05). Conclusion : These findings suggest that applying multiple SONO® ultrasound to the facial skin of healthy women for 4 weeks, can increase the skin elasticity and elasticity of deeper skin by supporting epidermal hydration and dermal collagen production.

각질층 환경변화에 따른 얼굴과 하박내측 피부의 탄력 특성 비교연구

장민열 ( Minyoul Chang ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.4

피부의 탄성은 주로 콜라겐, 탄력섬유 등이 풍부한 진피의 영향을 받는다. 하지만, 피부의 가장 바깥층인 각질층의 변화가 피부의 기계적인 특성에 어떠한 영향을 미치는지에 대해선 아직 명확하게 밝혀지지 않고 있다. 이에 본 연구에서는 새롭게 디자인한 시험방법을 통해 피부 깊이별 탄성 특성과 수화과정을 통한 각질층 변화가 피부 탄성 특성에 어떻게 영향을 주는지를 알아보았다. 또한 이 방법을 이용해 얼굴피부와 하박내측 피부의 특성을 비교 평가하였다. 25-40세 연령의 건강한 성인 10명을 대상으로, 피부 깊이별 탄성 특성을 연구하기 위해 Cutometer^Ⓡ의 음압을 다양하게 설정하여 얼굴피부와 하박내측 피부의 탄성 특성을 측정하여 비교 평가하였다. 음압은 100, 200, 300, 450 mbar로 구분하여 측정하였다. 또한 충분한 수화과정을 통해 각질층의 변화를 유도하고, 다양한 음압 조건에서 피부 탄성 특성을 측정하였다. 그 결과, 음압 조건을 달리함에 따라 피부 깊이에 따른 탄성 특성 측정이 가능함을 확인하였다. 그리고 하박내측 피부의 R7 값은 모든 음압조건에서 얼굴피부보다 통계적으로 유의하게 높았다(p < 0.05). 또한 수화과정에 의한 각질층의 변화는 피부탄성 특성에 영향을 줄 수 있음을 확인하였다. 특히 얼굴피부의 R7 값은 300 mbar 음압조건에서 수화전 상태의 피부에 비해 통계적으로 유의하게 변화하였다(p < 0.05). 그리고 전체적으로 얼굴피부가 하박내측 피부보다 수화과정에 더 크게 영향을 받고 있음을 알 수 있었다. 이러한 결과는 다양한 음압조건과 피부 수화 과정을 활용한다면, 각질층 및 표피층의 변화를 고려한 피부탄력특성을 평가할 수 있는 유용한 시험법이 될 것으로 사료된다. Skin elasticity has been known to be influenced by the change of dermal components such as collagen, elastic fiber, and glycosaminoglycans. However, it is unclear whether the uppermost epidermis may affect the mechanical characteristics of elasticity. In present study we tried to find the role of stratum corneum when determining the elastic property under skin bioengineering measurement with Cutometer^Ⓡ. A total of 10 healthy volunteers aged 25-40 years were investigated by the parameters reflecting the skin elasticity from face and volar forearm. Within various ranges of suction pressure, R0 (=Uf), R7 (=Ur/Uf) and R8 (=Ua) were obtained to explore the depth-associated elasticity. In addition, these parameters were re-measured after the skin was fully hydrated. As results, we found that the measurement of depth-associated elasticity was possible as using various suction pressure. And the R7 parameter was significantly lower from face than those from forearm in before hydration (p < 0.05). Also, we found that the hydration of stratum corneum could affect skin elasticity. Especially, the R7 parameter at 300 mbar suction pressure of face skin showed significantly increased values than that of before hydration (p < 0.05). Interestingly, measured data from the face before and after hydration manifested relatively higher variation than from the forearm. These results suggest that it is possible to evaluate the skin elasticity considering the changes of stratum corneum and epidermis by using various suction pressure and skin hydration.

모녀간 피부 특성의 상관관계에 대한 연구

조가영 ( Ga Young Cho ),김지연 ( Jee Yeun Kim ),염명훈 ( Myeong Hun Yeom ),조준철 ( Jun Chul Cho ),김종일 ( Jong Il Kim ),김윤범 ( Yoon Bum Kim ) 대한한방부인과학회 2012 大韓韓方婦人科學會誌 Vol.25 No.4

Objectives: It is well known that genetic factors and environmental factors play major role in aging skin. In general, son and daughter take after their parents in appearance. But comparability of skin properties in Korean family has not been well estimated. So we evaluated the correlation of skin properties in Korean mother-daughter pairs. Methods: 10 couples of mother and daughter pair(n=20) participate in this study. Mothers and daughters were aged 50.6±2.459 and 23.8±2.15 years, respectively. Skin moisture, sebum, skin color and elasticity is measured using non-invasive method: Corneometer CM825, Sebumeter(MPA580), Mexameter MX18, Cutometer MPA580. Statistical analysis program we used is Minitab 14 Korea. Results: The Skin moisture of mother group was significantly higher than daughter group. And skin elasticity(R2) of the daughter group was significantly higher than mother group. In the results of correlation analysis between mother and daughter pairs, skin elasticity(R2) had high positive correlation(r=0.729, p=0.026). But skin moisture, sebum, melanin index, erythema index and skin elasticity(R5) did not be confirmed a significant correlation. Conclusions: Skin elasticity(R2) is the only factor that showed high positive correlation significantly in mother and daughter pairs. Mother and daughter share the genetic factors and environmental factors that influence skin aging. So, mother`s aging pattern will be a good guide for anti-aging of daughters.

봉독이 피부 노화에 미치는 영향

김진명,김윤범,Kim, Jin-Myoung,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.2

Background and Objective : Increasing interest in anti-aging and anti-wrinkling agents for the skin has triggered the recent outflow of researches and studies in this field. This study was designed to investigate the effects of bee venom on skin wrinkling and skin aging by testing the skin wrinkling, skin elasticity, trans-epidermal water loss (TEWL), free radical level, anti-oxidative agent level, and skin tissue after infusion of bee venom on hairless mouse. Materials and Methods : Fifteen hairless mice aged between 36~40 weeks were divided randomly into 3 Group; the Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group. The Bee Venom Syringe Group were injected subcutaneously with bee venom (0.1cc in total) using an insulin syringe on three spots in the lumbar spine (one spot on the center and two spots 1~2cm to the side bilaterally). The Bee Venom Needle Group were pricked with bee venom-smeared acupuncture needles on three longitudinal spots in the lumbar spine each 1cm apart, after which the needles were removed 10 minutes later. The Control Group did not receive any form of intervention. All procedures took place thrice a week for four weeks, during which the mice were allowed free access to water and fodder. The mice were measured and compared in the weight, skin wrinkling scale, skin elasticity, and TEWL before and after the experiment. After the experiment, blood samples were taken to measure the free radical and anti-oxidative agent level, and the skin tissue was sliced for examination. Data was analyzed using the SPSS program (ver 12.0). The ANOVA analysis was used to compare and contrast the three groups, and t-test for paired samples was used to evaluate skin-wrinkling before and after experiment. The cut-off p-value of significance was set at p<0.05. Results : 1. Administration of bee venom did not cause serious weight loss or gain. 2. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed a decrease in skin wrinkling scale after intervention. Especially, the Bee Venom Syringe Group showed a significant decrease (p<0.05). 3. Compared to the control group, the Bee Venom Syringe Group and the Bee Venom Needle Group both showed an increase in skin elasticity. Especially, the Bee Venom Syringe Group showed a significant increase (p<0.05). 4. No significant change in TEWL was found in the mice in all the three groups before and after experiment. 5. Free radical level was normal in all 15 mice in all the three groups, and anti-oxidative agent was not significantly different across the three groups. 6. The Bee Venom Syringe Group, the Bee Venom Needle Group, and the control group did not show any significant difference in the thickness of epidermis and dermis, infiltration of inflammatory cells, and skin wrinkling. The epidermis layer was relatively better preserved in the Bee Venom Syringe Group as compared to the Bee Venom Needle Group and the control group. Conclusion : Direct injection of bee venom on the hairless mouse using a syringe was found to improve wrinkling of the skin and increase skin elasticity but did not show effectiveness on skin dryness due to water loss. The bee venom appears to have suppressive effects on skin wrinkling, one of the symptoms of skin aging, through a process independent of suppression of free radicals or increase of anti-oxidative agent.

글리콜산 필링후 건성 및 지성 피부의 유분, 수분과 피부 탄력도 평가

공희경,황인철 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.3

A glycolic acid peel is the typical one of chemical peels used to improve and smooth the texture of the facial skin using a chemical solution such as natural fruit acids. Glycolic acid is one of alpha hydroxy acids(AHAs) to remove old, dead, and sun-damaged surface skin cells, and promote healthier and softer skin. This study was conducted to evaluation the improvement effects in skin moisture, sebum(oiliness) and elasticity for dry and oily skin types after glycolic acid treatment. The 20 young ladies of 21.0±5.2 years old were assigned to two group according to their dry or oily skin types. And then skin moisture, sebum and elasticity in T-zone(forehead) and U-zone(cheek) using a non-invasive measuring method were measured after a glycolic acid peel treatment a week for 4 weeks. The results were as follows:1. The sebum content of T- and U-zone in dry skin group showed a significant increase with increasing glycolic acid peel treatments, while in oily skin group it was significantly decreased (p<0.05). 2. The moisture content of T- and U-zone in dry skin and of U-zone in oily skin group was increased with increasing glycolic acid peel treatments. But the moisture content of T-zone did not show significant difference according to repeated chemical peel treatments (p>0.05). 3. The elasticity content of T-zone and U-zone in dry skin group and of T-zone in oily skin group was significantly increased according to repeated chemical peel treatments. But the elasticity content of U-zone in oily skin group did not show according to repeated chemical peel treatments (p>0.05). 에스테틱 필링은 진피에 손상을 주지 않고 주기적으로 각질을 탈락 시켜줌으로써 피부 재생과 점차적인 개선 효과를 얻을 수 있어 광범위하게 많이 이용되고 있다. 본 연구는 임상에서 화학적 필링제로 가장 많이 사용되고 있는 글리콜산을 건성과 지성피부에 각각 4주간 적용하여 피부 유분, 수분, 탄력도의 변화를 관찰하였다. 실험 대상은 20대 여대생(평균 연령 21±5.2세)으로 건성과 지성피부 각각 10명씩 총 20명으로 하였고, 주 1회 간격으로 총 4회 글리콜산 필링 관리를 하였으며, 관리중의 피부상태 변화를 알아보기 위해 필링전, 2, 4회 필링후에 안면 피부 상태를 측정하였다. 글리콜산 필링후 유분량은 건성피부에서는 T존과 U존 모두 관리횟수가 증가하면서 유의한 증가를 보였지만, 지성피부에서는 유의한 감소를 나타내었다. 수분량은 건성피부에서는 T존과 U존 모두 관리횟수가 증가하면서 유의한 증가를 보였지만, 지성피부에서는 T존은 관리횟수에 따른 유의한 차이가 없었고, U존은 관리횟수가 증가하면 유의한 증가를 나타내었다. 탄력도는 건성피부에서는 T존과 U존 모두 관리횟수가 증가하면서 유의한 증가를 보였지만, 지성피부에서는 T존은 관리횟수가 증가하면 유의한 증가를 보였으나, U존은 유의한 차이가 없었다. 따라서 글리콜산 필링은 건성피부의 T존과 U존의 유분 증가, 수분 증가, 탄력 증가에 효과적이고, 지성피부의 유분 감소, U존의 수분 증가, T존의 탄력 증가에 효과적인 것으로 사료된다

Liquid collagen from freshwater fish skin ameliorates hydration, roughness and elasticity in photo-aged skin: a randomized, controlled, clinical study

Joong Su Lee,Yeo Cho Yoon,Ju Myung Kim,Yun-Ho Kim,Young-Hee Kang,Yong Chul Shin 한국영양학회 2024 Nutrition Research and Practice Vol.18 No.3

BACKGROUND/OBJECTIVES: Collagen is commonly used in diverse forms as a functional component in skincare products. On the other hand, the effects of collagen on human skin are controversial. Dietary collagen hydrolysates from freshwater Pangasius hypophthalmus fish skin ameliorated photo-aged skin of hairless mice. This study conducted a randomized, double-blind, placebo-controlled clinical trial to determine if liquid fish collagen (Collagen-Tripep20™, Tripep20) as a drink strengthens skin health and quality. SUBJECTS/METHODS: In this clinical trial, 85 subjects aged 35–60 yrs were diagnosed with photo-aged skin. Eighty-five subjects were randomized to receive either Tripep20 (n = 44) or placebo (n = 41). Seventy-eight subjects fully participating for a 12-week period consumed 1,000 mg of Tripep20 (n = 41) or placebo (n = 37) in a 50-mL bottle as a daily drink. The intend-to-treat and per-protocol populations were 85 and 78, respectively. Skin hydration, wrinkles, and elasticity were assessed at 0 (baseline), 6, and 12 weeks during the study period. RESULTS: Skin hydration in the Tripep20 group was significantly higher from 6 weeks (P < 0.001) than the baseline. After 12 weeks, the Crow’s-feet visual score and skin roughness (R<SUB>a</SUB>, R<SUB>q</SUB>, and R<SUB>max</SUB>) were significantly improved in the Tripep20 group than in the placebo group (P < 0.05). Consuming liquid collagen Tripep20 greatly enhanced skin elasticity (Gross R2, Net R5, and Biological elasticity R7) in 6 weeks compared to the placebo group. The Tripep20 group showed a significant increase in skin elasticity from the baseline after 6 and 12 weeks (P < 0.001). Neither abnormal symptoms nor adverse events were encountered during the study period in subjects ingesting Tripep20 or placebo. The changes in parameters related to hematology and clinical chemistry were within the normal ranges. CONCLUSION: Oral consumption of liquid collagen Tripep20 was safe and well-tolerated. The results of this study show that freshwater fish-derived liquid collagen Tripep20 can be used as a healthy functional food ingredient to improve skin moisturizing, anti-wrinkling, and elasticity in an aging population.

연령과 성별에 따른 비침습적 방법을 이용한 피부측정값의 비교

박경순 ( Kyoung Soon Park ),오수정 ( Su Jung Oh ),강수경 ( Su Kyung Kang ) 한국미용학회 2009 한국미용학회지 Vol.15 No.2

This study was to compare the measurements of skin moisture, elasticity and even grain between men and women depending upon their age. Focusing on total 203 residents (male and female; age ranging from 10 to 49) living in Gwangju metropolitan city and Jeonnam province, used ARAMO-TS. It was found that all our subjects hereof scored the following mean points in each skin measurement item: 40.56±4.71 (in moisture), 42.90±11.62 (skin elasticity), and 31.81±7.37 (even grain). Depending upon sex, it was found that women scored higher mean points in the moisture and even grain of skin surface than men (p<0.05). Depending on age, it was found that 10`s group scored highest mean point (42.22±4.33) in the content of moisture among others, and the older age was in significant correlations with the lower moisture content of skin. And it was found that 10`s group scored the highest points in skin elasticity and even grain among others, which was followed by 20`s, 40`s and 30`s group respectively. Here, it is found that 30`s group has worst conditions of skin elasticity and even grain among others (p<0.01). Depending on age and sex, it was found that female group in 10`s and 20`s had significantly better skin elasticity than male group at same age, while female group in 30`s and 40`s had significantly lower skin roughness than male group at same age (p<0.01).

노인여성의 피부문제와 비침습적 피부측정값의 상관관계

박정연 ( Park Jeong Yeon ),강수경 ( Kang Su Kyung ) 대한미용학회 2012 대한미용학회지 Vol.8 No.4

This study has been attempted to identify the relevance of skin problems of which elderly women are aware in some areas with some measurements on skin using non-invasive methods. The results are as follows: In terms of skin problems perceived by the female senior citizens, ``dry skin´ was the highest, followed by ``lentigo``, ``itching`` and ``scaling``. Upon looking at the differences of measurements on skin depending on degree of skin problems that they realized, moisture in uredo, moisture, lipid and TEWL in dry skin, TEWL in scaling and moisture, and melanin in solar lentigo showed statistically significant differences, respectively. Through above results, the relevance was identified by examining the skin problems which elderly women realized and the differences of moisture, lipid, TEWL, elasticity, melanin and erythema on surface of skin based on the degree of skin problem. Based on this, this study can be used as the basic material required to develop cosmetics and skin care program targeting the seniors.

연구논문 : 여성의 피부건강상태 및 관련 생활행태의 연구

홍진이 ( Jin I Hong ),김주덕 ( Ju Duck Kim ) 한국미용학회 2007 한국미용학회지 Vol.13 No.3

The purpose of this study was to examine what internally and externally affected skin care in routine life in an attempt to help improve the daily life habits of women and delay their skin aging. The subjects in this study were 379 female adults from Seoul, Bundang and Chuncheon. They were 97 female at the age of 20 and up. After their medical history and facial skin were checked, a survey was conducted. The findings of the study were as below: 1. Concerning in which part of the facial skin they were interested, they were most intrigued by pigmentation like liver sports or freckles (21.35%), followed by elasticity(20.3%), wrinkles(18.4%), dryness(15.8%), pore size(10.7%), excessive sebum excretion(6.2%) and others. 2. The most widely used basic cosmetic was toner and emulsion(17%), followed by sun block(14%), nutrition cream(14%), eye cream(12%), moisturizing cosmetics(11%), wrinkle-improving cosmetics(7%) and whitening cosmetics(5%). 3. As for skin care, the women who took care of their own skin by using cosmetics, etc., constituted the largest group(55.4%). The second largest group(11.3%) used skin-care clinics, and the third greatest group(5%) visited dermatologists. The fourth largest group(28.2%) didn`t take care of their skin. The most frequently used material for skin care was a mask pack(44.3%), and those who were less educated, whose monthly income was smaller and who were younger took care of their skin in person. 4. Regarding life habits, more than half(62.8%) slept for six to seven hours a day, and many(54.1%) took four or five cups of water a day. 5. When their facial skin was evaluated, moisture values were higher among the women who took six or seven cups of water a day, who slept for six to eight hours, who didn`t have a coffee, who were jobless, who were married and who got regular exercise. 6. As to oil state, those who worked out were moist but not oily in the face. Specifically, the women who had a job and who found themselves under pressure were oilier in the forehead. 7. The skin elasticity was stronger among those who worked out on a regular basis, who received skin-care treatment from experts, who slept for six to seven hours, who didn`t have a coffee, and who were unemployed.

정제된 스피큘을 함유한 화장품의 경피흡수력 및 노화피부 개선에 미치는 영향

한도숙,김기숙,강상모 한국미용학회 2020 한국미용학회지 Vol.26 No.4

Spicules is a physical exfoliator that is known in academia that induces normal keratination cycles and further promotes skin metabolism and skin regeneration. In addition, based on the structural feature with its fine pores spicules, experimental attempts to use it as a percutaneous delivery system for cosmetics will be meaningful. To address this study has two-folded purposes: verifying the percutaneous absorbance of cosmetics manufactured by a combination of purified spicules and functional active ingredients, and identifying how much skin improvement the spicules-containing functional cosmetics through observe in those changes after applying to the actual skin. For these aims the percutaneous absorbance of niacinamide was evaluated on the artificial membrane similar to that of human skin was compared with the general functional cosmetics and the functional cosmetics containing 1% weight of purified spicules. In addition, clinical trial was conducted for four weeks targeting the total number of twenty women. As for the clinical trials experimental groups involves: a group with placebo cosmetics (C), general functional cosmetics (T1) and spicules-containing functional cosmetics (T2). As a result of the percutaneous absorption-testing of niacinamide showed that T2 significantly increased amount of absorptive & absorptivity of naiacinamide over time (p<.001). Furthermore, resulted in different changes on the skin, depending on their respective groups. In particular, the T2 has obtained the following statistically significant results: a decrease in eye bags laxity (p<.001), oral angle laxity (p<.001) and TEWL (p<.001), an increase in skin elasticity (p<<).001, skin density (p<.001) and skin brightness (p<.001). Thus, the purified spicules has been proven as material that helps absorb the active ingredients of cosmetics for improve aging skin.