아토피피부염을 가진 24개월 미만의 소아에서 집먼지진드기 특이 IgE 항체의 양전

박미란 ( M Iran Park ),이현영 ( Hyun Young Lee ),이상일 ( Sang Il Lee ),김지현 ( Ji Hyun Kim ),안강모 ( Kang Mo Ahn ) 대한천식알레르기학회 2013 Allergy Asthma & Respiratory Disease Vol.1 No.4

Purpose: A considerable number of children with atopic dermatitis (AD) is known to be sensitized with respiratory allergen and developed to respiratory allergic disease. We aimed to determine the time of sensitization to house dust mite (HDM) allergen and identify the risk factors of sensitization in children with AD. Methods: Data from 159 children with AD aged less than 24 months and with no sensitization to HDM were analyzed retrospectively. Disease severity, total IgE, specific IgE levels to HDM and food allergens were assessed. They were followed to monitor positive conversion of specific IgE against HDM and development of allergic respiratory diseases. The specific IgE was considered as positive when its level was over 0.69 kU/L. Results: The 44.7% of children with AD were sensitized to HDM. The 50% of sensitization to HDM occurred at the median age of 52 months using survival analysis. The severity of atopic dermatitis (six area, six sign in atopic dermatitis score>15; hazard ratio, 2.62) and high peak total IgE in 2 years old (hazard ratio, 1.58) was associated with an increased risk of sensitization to HDM. Sensitization to food allergen, family history of allergic disease and total IgE of 1st visit of hospital were not associated with early sensitization to HDM. Conclusion: The sensitization of HDM of children with AD occurred at the median age of 52 months. The risk factors of sensitization to HDM were AD severity and total IgE level. Children with severe AD need to control the indoor environment before 24 months of age.

아토피피부염을 가진 24개월 미만의 소아에서 집먼지진드기 특이 IgE 항체의 양전

박미란 ( Mi Ran Park ),이현영 ( Hyun Young Lee ),이상일 ( Sang Il Lee ),김지현 ( Ji Hyun Kim ),안강모 ( Kang Mo Ahn ) 대한소아알레르기호흡기학회 1991 소아알레르기 및 호흡기학회지 Vol.1 No.4

Purpose: A considerable number of children with atopic dermatitis (AD) is known to be sensitized with respiratory allergen and developed to respiratory allergic disease. We aimed to determine the time of sensitization to house dust mite (HDM) allergen and identify the risk factors of sensitization in children with AD. Methods: Data from 159 children with AD aged less than 24 months and with no sensitization to HDM were analyzed retrospectively. Disease severity, total IgE, specific IgE levels to HDM and food allergens were assessed. They were followed to monitor positive conversion of specific IgE against HDM and development of allergic respiratory diseases. The specific IgE was considered as positive when its level was over 0.69 kU/L. Results: The 44.7% of children with AD were sensitized to HDM. The 50% of sensitization to HDM occurred at the median age of 52 months using survival analysis. The severity of atopic dermatitis (six area, six sign in atopic dermatitis score>15; hazard ratio, 2.62) and high peak total IgE in 2 years old (hazard ratio, 1.58) was associated with an increased risk of sensitization to HDM. Sensitization to food allergen, family history of allergic disease and total IgE of 1st visit of hospital were not associated with early sensitization to HDM. Conclusion: The sensitization of HDM of children with AD occurred at the median age of 52 months. The risk factors of sensitization to HDM were AD severity and total IgE level. Children with severe AD need to control the indoor environment before 24 months of age. (Allergy Asthma Respir Dis 2013;1:350-356)

한국인 성인 알레르기 환자에서 국내산 농산물에 대한 감작률 및 교차반응성

윤성호 ( Sung Ho Yoon ),강영미 ( Young Mi Kang ),예영민 ( Young Min Ye ),김승현 ( Seung Hyun Kim ),서창희 ( Chang Hee Suh ),남동호 ( Dong Ho Nahm ),박해심 ( Hae Sim Park ) 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.4

Background: Agricultural products have been mainly consumed food in Korea. However, there have been no published data to observe the IgE-sensitization rate, cross-reactivity and allergenic risks to home-made agricultural food in adult allergy patients. Objective: The aim of this study was to evaluate the IgE sensitization rate and cross-reactivity to home-made agricultural products and to establish a basic data for planning treatment and prevention methods of food allergy in adult allergy patients. Method: Five thousand three hundred forty allergy patients complaining of various allergic diseases and visiting the Allergy Clinic of Ajou University Hospital were enrolled. Skin prick tests were performed with home-made agricultural products including soybean, corn, buckwheat, chestnut, rye grain, potato, nut, wheatflour, carrot, onion and rice. If A/H ratio for each agricultural product showed more than 2+ on skin prick test, it was considered a positive response. ELISA inhibition tests were performed for cross-reactivity to agricultural products. Result: The sensitization rate to home-made agricultural products ranged from 2.2% to 9.5% in adult allergy patients. Sixty-five (3.8%) and sixty-four (3.7%) out of the one thousand seven hundred sixteen patients showed positive responses (A/H≥2+) to soybean and corn, positive responses to buckwheat and chestnut were noted in 84 (4.8%) and 66 (3.8%) patients respectively, followed by rye grain (9.5%), potato (5.7%), nut (5.4%), wheatflour (5.1%), carrot (5.1%), onion (3.3%) and rice (2.2%). Wheat flour and rye grain inhibited IgE binding to each other. Soybean, corn, Hop. J, wheatflour and rye grain extracts inhibited IgE binding to potato extracts in potato sensitized subjects sera. There was a strong inhibition between corn and soybean extract. Conclusion: The sensitization rate to major agricultural products ranges from 2.2% to 9.5% in adult allergy patients, and buckwheat and soybean were the most prevalent ones among the home-made agricultual products in this country. Agricultural products-specific IgE antibody that cross reacted with other agricultual products can cause allergic reaction in foods allergy. (Korean J Asthma Allergy Clin Immunol 2005;25:269-275)

성인 알레르기 환자에서 자연산과 유전자 변형 감자에 대한 감작률과 특이 IgE 항체 측정 및 알레르겐 성상 비교

윤성호 ( Sung Ho Yoon ),김현미 ( Hyun Mi Kim ),예영민 ( Young Min Ye ),강영미 ( Young Mi Kang ),서창희 ( Chang Hee Suh ),남동호 ( Dong Ho Nahm ),김승현 ( Seung Hyun Kim ),박해심 ( Hae Sim Park ) 대한내과학회 2005 대한내과학회지 Vol.69 No.6

목적 : 최근 유전자 변형 기술의 발달로 유전자 변형 음식의 생산과 소비가 급증하고 있는 추세이다. 본 연구에서는 국내에서 섭취가 많고 또한 유전자 변형 음식물 중 하나인 감자의 주요 알레르겐 성분을 규명하고 유전자 변형에 의한 알레르기 유해능을 평가하고자 하였다. 방법 : 각종 알레르기 질환으로 아주대학교 알레르기 클리닉을 방문한 15세 이상의 성인 1,886명을 대상으로 자연산 감자와 유전자 변형 감자 항원으로 피부단자시험을 실시하였고, 각각의 항원에 대해 면역효소법으로 혈청 내 특이 IgE 항체치를 측정하여 비교하였으며 면역효소억제시험으로 각 감자 항원에 대한 IgE의 특이성을 관찰하였다. 또한 특이 IgE 항체치가 높은 환자들의 혈청으로 IgE-Immunoblot 분석을 실시하여 감자내 주요 알레르겐을 규명하여 유전자 변형에 따른 차이가 있는지를 조사하였다. 결과 : 국내 성인에서 자연산과 유전자 변형 감자에 대한 감작률은 모두 5.7%이었으며, 특이 IgE 항체는 두 감자 항원간에 유사한 결과를 보였다. 또한 면역효소억제시험에서 강한 억제 양상을 보였으며 두 항원간에 알레르기 성상은 유사하였다. 각각의 감자에서 1개의 공통적인 주요 알레르겐(45 kDa) 성분을 규명하였으며 두 감자 항원간에 차이는 없었다. 결론 : 본 연구의 결과로 볼 때 감자의 경우 유전자 변형 공정에 따른 알레르기 유해성의 증가는 가능성이 낮을 것으로 생각한다. Background : The aim of this study was to evaluate the prevalence of IgE sensitization and allergic risk of genetically modified (GM) potato compared with wild one in adult patients with various allergic diseases. Methods : One thousand eight hundred eighty eight allergy patients visited Ajou University hospital and 38 healthy controls were enrolled. Skin prick tests were performed with wild and GM extracts. Phosphinothricin N-acetyltransferase (PAT) and neomycin phosphotransferase (NPT) gene was inserted in GM potato. Serum specific IgE level to the two potato extracts was measured by ELISA and their binding specificities were confirmed by ELISA inhibition test. IgE binding components in both wild and GM potato extracts were identified by SDS-PAGE and IgE-immunoblot. Results : One hundred eight patients (5.7%) showed positive responses (A/H ≥ 2+) on skin prick test to both wild and GM potatoes. Serum specific IgE was detectable in 50~88% among the positive reactors on skin prick test. ELISA inhibition tests showed similar inhibition pattern between wild and GM. Fourteen IgE binding components within wild potato and nine IgE binding components within GM potato with similar binding patterns, of which three major allergens in wild (26, 34, 45 kDa) and one (45 kDa) in GM one were noted. Conclusions : The sensitization rates to wild and GM potato extracts were 5.7% respectively, in adult allergy patients and one common major allergen (45 kDa) was identified. It is speculated that genetic manipulation of the potato did not increase allergenic risk.(Korean J Med 69:651-659, 2005)

Changes in Sensitization Rate to Weed Allergens in Children with Increased Weeds Pollen Counts in Seoul Metropolitan Area

김주화,오재원,Ha-Baik Lee,Seong-Won Kim,Im-Joo Kang,Myung-Hee Kook,김봉성,Kang-Seo Park,백혜성,김규랑,최영진 대한의학회 2012 Journal of Korean medical science Vol.27 No.4

The prevalence of allergic diseases in children has increased for several decades. We evaluated the correlation between pollen count of weeds and their sensitization rate in Seoul, 1997-2009. Airborne particles carrying allergens were collected daily from 3 stations around Seoul. Skin prick tests to pollen were performed on children with allergic diseases. Ragweed pollen gradually increased between 1999 and 2005, decreased after 2005 and plateaued until 2009 (peak counts, 67 in 2003, 145 in 2005 and 83 grains/m3/day in 2007). Japanese hop pollen increased between 2002 and 2009 (peak counts, 212 in 2006 and 492 grains/m3/day in 2009). Sensitization rates to weed pollen, especially ragweed and Japanese hop in children with allergic diseases, increased annually (ragweed,2.2% in 2000 and 2.8% in 2002; Japanese hop, 1.4% in 2000 and 1.9% in 2002). The age for sensitization to pollen gradually became younger since 2000 (4 to 6 yr of age, 3.5% in 1997 and 6.2% in 2009; 7 to 9 yr of age, 4.2% in 1997 and 6.4% in 2009). In conclusion,sensitization rates for weed pollens increase in Korean children given increasing pollen counts of ragweed and Japanese hop.

Changes in Sensitization Rate to Weed Allergens in Children with Increased Weeds Pollen Counts in Seoul Metropolitan Area

Kim, Joo-Hwa,Oh, Jae-Won,Lee, Ha-Baik,Kim, Seong-Won,Kang, Im-Joo,Kook, Myung-Hee,Kim, Bong-Seong,Park, Kang-Seo,Baek, Hey-Sung,Kim, Kyu-Rang,Choi, Young-Jean The Korean Academy of Medical Sciences 2012 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.27 No.4

<P>The prevalence of allergic diseases in children has increased for several decades. We evaluated the correlation between pollen count of weeds and their sensitization rate in Seoul, 1997-2009. Airborne particles carrying allergens were collected daily from 3 stations around Seoul. Skin prick tests to pollen were performed on children with allergic diseases. Ragweed pollen gradually increased between 1999 and 2005, decreased after 2005 and plateaued until 2009 (peak counts, 67 in 2003, 145 in 2005 and 83 grains/m<SUP>3</SUP>/day in 2007). Japanese hop pollen increased between 2002 and 2009 (peak counts, 212 in 2006 and 492 grains/m<SUP>3</SUP>/day in 2009). Sensitization rates to weed pollen, especially ragweed and Japanese hop in children with allergic diseases, increased annually (ragweed, 2.2% in 2000 and 2.8% in 2002; Japanese hop, 1.4% in 2000 and 1.9% in 2002). The age for sensitization to pollen gradually became younger since 2000 (4 to 6 yr of age, 3.5% in 1997 and 6.2% in 2009; 7 to 9 yr of age, 4.2% in 1997 and 6.4% in 2009). In conclusion, sensitization rates for weed pollens increase in Korean children given increasing pollen counts of ragweed and Japanese hop.</P>

연구논문 : 비이온성 계면활성제에 의한 mouse 피부층의 형태 및 Lectin변화 연구

최정숙 ( Jeung Suk Choi ),이선주 ( Seun Joo Lee ) 한국미용학회 2003 한국미용학회지 Vol.9 No.3

Ultraviolet A Induces Immunosuppression, Protection or Memory Enhancement Depending on Dose, while Ultraviolet B is Immunosuppressive and Tolerogenic over a Large Dose Range

Halliday, Gary M.,Byrne, Scott N. Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

UVR-induced immunosuppression contributes to skin cancer. The aim was to construct accurate dose response curves for primary and secondary contact sensitivity for solar-simulated UVR (ssUVR; 290-400nm), UVA and UVB as the role of UVA in immunosuppression is controversial. We used a xenon arc source. The mice were immobilised, enabling accurate dosing. C57BL/6 mice were immunosuppressed at half the dose of ssUVR required to cause sunburn but not by higher doses (up to the sunburn dose). Thus, ssUVR causes systemic immunosuppression only over a narrow, low dose range. UVA caused suppression at low but not high doses whereas UVB induced immunosuppression at all doses tested. 8 weeks later the mice were resensitised to assess tolerance. Mice exposed to the minimum immunosuppressive dose of ssUVR prior to primary sensitisation were tolerant to re-sensitisation. However, at higher doses of ssUVR, these mice were protected from tolerance. Interestingly, while low doses of UV A caused immunosuppression, even lower doses enhanced the response to the second sensitisation. Higher doses of UVA had no affect. UVB induced tolerance in a dose related manner. Thus, ssUVR only induces immunosuppression and tolerance over a narrow dose range. Both UVA and UVB are immunosuppressive at this dose, while higher doses of UVA protect from the suppressive effects of UVB. Surprisingly very low doses of UVA enhanced memory development. Thus UVR has complex effects on the immune system depending on dose and spectrum.

Impact of pretransplant rituximab induction on highly sensitized kidney recipients

Young Hae Song,Kyu Ha Huh,Yu Seun Kim,Hyung Soon Lee,Myoung Soo Kim,Soo Jin Kim,Hyun Jung Kim,Soon Il Kim,Dong Jin Joo 대한외과학회 2012 Annals of Surgical Treatment and Research(ASRT) Vol.82 No.6

Purpose: Highly sensitized patients with a high level of panel reactive antibody (PRA) experience more episodes of antibody-mediated rejection (AMR) and poorer graft survival than non-sensitized patients. Rituximab is a well-known monoclonal anti-CD20 antibody that causes the depletion of B lymphocytes. The aim of this study was to compare a rituximab-administered and a non-administered group of highly sensitized recipients. Methods: Forty-three kidney recipients with a PRA level of ≥50% were included. Sixteen (group R) received one dose of rituximab at 2 days prior to transplantation and 27 patients (group NR) did not. Results: Patients’ demographics, such as age, sex, dialysis duration, and type of immunosuppressive agent were not different in the two groups. No side effects due to rituximab administration were observed in group R. Class I PRA of group R (75.6 ± 37.7%) was higher than that of group NR (45.7 ± 35.8%, P = 0.013). More acute rejection episodes occurred within 1 year after transplantation in group NR but the difference between the groups was not significant (18.8% in group R vs. 29.6% in group NR, P = 0.631). However, two AMR episodes occurred only in group NR. Renal functions were not different in the two groups. In group R, CD19 and CD20 rapidly decreased 2 days after rituximab infusion. Furthermore, the administration of rituximab was not linked to acute rejection. Conclusion: To confirm the long-term anti-rejection and beneficial effects of rituximab, further studies should be performed with a larger cohort. In conclusion, rituximab administration 2 days prior to transplantation is both effective and safe.