소아에서 연령군에 따른 집먼지진드기 주항원 ( Der f 1 과 Der f 2 ) 에 대한 특이 IgE 항체의 변화

남동호(Dong Ho Nahm),박중원(Jung Won Park),홍천수(Chein Soo Hong),이수영(Soo Young Lee),이기영(Ki Young Lee) 대한소아알레르기호흡기학회 1995 소아알레르기 및 호흡기학회지 Vol.5 No.2

N/A To evaluate the early IgE antibody response to house dust mite in child, we measured the specific IgE antibodies to whole body extract (WBE), of D. farinae, and two major allergens, Der f I and Der f II by ELISA (enzyme-linked immunosorbentassay) in sera from 173 children less than 16 years old who visited Allergy Clinic in Severance Hospital with the chief complains of respiratory and/or dermatologic allergic disease. 1. The levels of WBE specific IgE antibody were steadily increased accoding to the increase of age groups, and there was the significant difference between less than four years of age groups, (0.062±0.129 vs 0.500±0.361; t-test, p<0.05). 2. The levels of Der f I and Der f II specific IgE antibodies were also steadily increased by age, and there were noted significant differences between before and after years of age groups (in Der f I: 0.027±0.030 vs 0.082±0.076, in Der f II; 0.334±0.267 vs respectively, t-test, p<0.05). 3. In all age groups over 4 year, the responses of specific IgE antibody to Der f II were significantly higher than the responses to Der f L. In conclusion, these results suggest that IgE antibody response to house dust mite significantly rise after 4 year of age and Der f II may be more important than Der f I in the development of early IgE response to house dust mite antigen in Korean children.

자가면역성 갑상선질환과 알레르기

남동호 ( Dong Ho Nahm ) 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.3

전신성 홍반성 낭창 환자 혈청내 항뇌신경세포 항체에 관한 연구

남동호(Dong Ho Nahm),박중원(Jung Won Park),홍천수(Chein Soo Hong),조상래(Sang Nae Cho),김태승(Tae Seung Kim) 대한내과학회 1992 대한내과학회지 Vol.43 No.4

N/A Background: Neuropsychiatric disorders caused by lupus activity occur during the course of the illness in about 50% of patients with systemic lupus erythematosus (SLE). And neuropsychiatric disorder is a poor prognostic sign and frequent cause of death in lupus patients. Recently some evidences suggest that autoantibodies directed against neuronal cell may have pathogenic significance in neuropsychiatric lupus. The present study was undertaken to characterize further nature of antineuronal antibody in SLE sera and to prove the association between antineuronal antibody and neurapsychiatric disorders in lupus. Methods: Sera from 61 patients with SLE (female 58, male 3) and 20 health control were examined for IgG antineuronal antibody by indirect immunofluorescence method using the frozen section of human brain (cortical portion of parietal lobe), Absortion experiments with 10 sera of high antineuronal antibody titer (≥1:10) by cardiolipin, asialoGM1, CNS neurofilament, myelin basic protein, ceramide and galactocerebroside were done to evaluate cross-reaction between purifed protein and lipid antigens and fluorescent IgG antineuronal antibody. Results: Fluorescent lgG antineuronal antibody was detected in 64% (39/61) of SLE patients and 5% (1/20) in health control, 3 types of fluorescent patterns of neuronal staining were observed. Those were homogenous pattern (21 cases), ring pattern (3 cases) and mixed pattern (18 cases). SLE patients with neuropsychiatric disorders had high titer (≥l:10) of fluorescent IgG antineuronal antibody more frequently than those without neuropsychiatric disorders (14/29, 48% vs 4/32, 12.5%; p<0.05). Lymphocyte counts of the peripheral blood in SLE patients with neuropsychiatric disorders were significantly lower than those of SLE patients without neuropsychiatric disorder (788/mm3 vs 1453/ mm3; p<0.05). Absorption experiments showed the significant changes in immunofluorescent patterns or immunofluorescent grades by asialo GM1, CNS-neurofilament and cardiolipin. Conclusion: IgG antineuronal antibody was high titer in neuropsychiatric lupus and absorption experiment suggested the cross-reactivity of antineuronal antibody with cardiolipin, asialo GM1, CNS-neurofilament.

아토피성 및 비아토피성 기관지천식 환자의 혈청내 항행항체 및 항갑상선 자가항체의 양성률 비교

남동호(Dong Ho Nahm),김선신(Sun Sin Kim),이수걸(Soo Keol Lee),박해심(Hae Sim Park) 대한천식알레르기학회 2000 천식 및 알레르기 Vol.20 No.2

N/A Background and objectives : There have been several reports showing increased prevalences of various autoantibodies in non-atopic asthma compared to atopic asthma. And the possible involvement of autoimmune mechanisms in the pathogenesis of non-atopic asthma has been suggested. To evaluate this hypothesis, we compared the prevalence of circulating autoantibodies in sera from patients with non-atopic and atopic asthma. Subjects and methods : Sera samples from 23 patients with non-atopic asthma showing no visible skin reaction to 50 common inhalant allergens and serum total IgE levels below 200 IU/ml, 23 patients with atopic asthma who showing positive skin prick tests and positive allergen bronchoprovocation tests, 34 age-matched healthy volunteers, and 20 patients with systemic lupus erythematosus were enrolled. We screened IgG antinuclear antibody by indirect immunofluorescence test using HEp-2 cell, and IgG autoantibodies to thyroglobulin and thyroid peroxidase by radioimmunoassay. Results IgG antinuclear antibodies were found in 1 (3%) of 34 healthy volunteers, 3 (13%) of 23 patients with non-atopic asthma, none of 23 patients with atopic asthma, 19 (95%) of 20 patients with systemic lupus erythematosus. IgG autoantibodies to thyroglobulin or thyroid peroxidase were found in 2 (6%) of 34 healthy volunteers, 2 (9%) of 23 patients with non-atopic asthma, 1 (4%) of 23 patients with atopic asthma, and 4 (25%) of 20 patients with systemic lupus erythematosus. Conclusion : There was no evidence for increased prevalence of autoantibodies to nuclear and thyroid antigen in sera from atopic and non-atopic asthmatics compared to healthy controls.

비용 조직에서의 집먼지진드기에 대한 IgE 항체 검출

강지원,남동호,서경식,김희연,박해심 ( Ji Won Kang,Dong Ho Nahm,Kyung Sik Suh,Hee Yeon Kim,Hae Sim Park ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Objective '. In order to confirm the local production of total and specific IgE antibodies in the nasal polyp tissues. Material and method: We measured total IgE and house dust mite(Dermatophagoides pteronpssinus .' DP)-specific IgE antibody using enzyme-linked immunosorbent assay(ELISA) in the supernatant of nasal polyp homogenates from 72 subjects undergoing nasal polypectomy. The subjects were divided into three groups according to skin reactivity to DP: 20 strongly atopic subjects to group I(mean wheal diameter) 3mm), 19 weakly atopic subjects to group II (mean wheal diameter 1-3mm) and 33 negative skin responders to group III. Result: Group I showed significantly higher levels of total and DP-specific IgE levels in the nasa

아토피피부염 치료 실태에 관한 다기관 설문조사

이동훈 ( Dong Hun Lee ),도은진 ( Eun Jin Doh ),이진영 ( Jin Young Lee ),박양 ( Yang Park ),오재원 ( Jae Won Oh ),이미희 ( Mi Hee Lee ),홍수종 ( Soo Jong Hong ),이소연 ( So-yeon Lee ),박준수 ( Joon Soo Park ),남동호 ( Dong-ho Nahm 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.4

Purpose: The effective management of atopic dermatitis (AD) adjusted to individual clinical courses and demands can be challenging to both patients and physicians. Understanding of actual situations, experienced and perceived by patients with AD and their caregivers, is essential to improve clinical outcomes and satisfaction in real practice.Methods: This multicenter survey was conducted in patients with AD or their caregivers from 9 centers with questionnaires on diagnosis and management of AD.Results: A total of 324 patients and caregivers participated in the study. Most of the AD cases were initially diagnosed by physicians (80.6%), followed by self-diagnosis. Patients and caregivers thought that allergic substances, such as house dust mites, food, and pollutants, are responsible for AD development; moisturization, environmental control, and improvement of the body constitution are important for AD management. Allergy tests were performed in 194 patients (59.9%), but allergen avoidance strategy was instructed in only 81 subjects (41.8%). Major topical medications were steroids (81.8%) and topical immunomodulators (34.3%), while systemic medications were steroids (42.6%), antihistamines (36.4%), and cyclosporins (2.8%). One hundred eighty-one subjects (55.9%) had received complementary alternative medicine, including Oriental medicine. Many subjects desired to receive individualized management, use of specialized institutions for AD as well as evidence-based, effective, sustainable treatment.Conclusion: Our findings suggest that there may still be an unmet need for patients with AD in real practice. Personalized, evidence-based, and multidisciplinary approaches, including patient education, should be implemented for good outcomes. (Allergy Asthma Respir Dis 2016:4:271-275)

Toluene diisocyanate 에 의한 직업성천식 환자의 객담내 호중구 활성화의 증거

김희연,남동호,박해심 ( Hee Yeon Kim,Dong Ho Nahm,Hae Sim Park ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3

Background: Toluene diisocyanate (TDI) is the most prevalent agent to cause occupational asthma (OA) in Korea. The pathogenic mechanism of TDI-induced OA is still unclear. Involvement of both immunological and non-immunologicaI mechanisms have been suggested. Objeetive '. To evaluate a possible role of neutrophil in the development of TDI-asthma. Object and method: Myeloperoxidase (MPO) as a neutrophil activation marker in both serum and induced sputum, and IL-8 in induced sputum were measured. Induced sputa and sera were collected from 15 TDI-induced OA patients (classified to group I) during TDI- bronchoprovocation test and were compared with those from 11 asthmatic subjects with negative TDI-bronchoprovocation test (group II). MPO levels were measured by radioimmunoassay, IL-8 levels, by enzyme linked immunosorbent assay and albumin levels, by nephelometry. Sputum MPO and IL-8 levels were presented as a ratio to albumin. Result: Serum MPO level tended to decrease during the TDI-bronchoprovocation test in two groups, but no statistical significance was reached (p>0.05). However, the ratios of MPO (the ratio of MPO level measured at 30 min to MPO level at baseline, and the ratio MPO level measured at 360 min to MPO baseline) in group I were significantly lower than group II (p=0.004, p=0.03 respectively). The IL-8/albumin and MPO/albumin levels in induced sputum from group I were significantly increased after the TDI-bronchprovocation test in comparison to the baseline value which was obtained before the bronchoprovocation test (p=0.0l, p=0.02 respectively). There was a significant correlation between the percent increase of IL-8/albumin and the MPO/albumin in induced sputum (r=0.89, p<0.05). Conclarsiog '. These findings suggest a possible involvement of neutrophil in the development of bronchoconstiction after the TDI exposure, and IL-8 might contribute to neutrophil recruitment to airway mucosa. Further investigation will be needed to investigate mechanism of neutrophil activation in the pathogenesis af TDI-induced OA.

부산지역 호흡기 알레르기 환자에서 흡입 알레르겐에 대한 감작률

김경호 ( Kyung Ho Kim ),김경태 ( Kyung Tae Kim ),이수걸 ( Soo Keol Lee ),박해심 ( Hae Sim Park ),이영목 ( Young Mok Lee ),남동호 ( Dong Ho Nahm ),손춘희 ( Choon Hee Son ),양두경 ( Doo Kyung Yang ),노미숙 ( Mee Sook Roh ),최필조 ( 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.1

한국인 성인 알레르기 환자에서 국내산 농산물에 대한 감작률 및 교차반응성

윤성호 ( Sung Ho Yoon ),강영미 ( Young Mi Kang ),예영민 ( Young Min Ye ),김승현 ( Seung Hyun Kim ),서창희 ( Chang Hee Suh ),남동호 ( Dong Ho Nahm ),박해심 ( Hae Sim Park ) 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.4

Background: Agricultural products have been mainly consumed food in Korea. However, there have been no published data to observe the IgE-sensitization rate, cross-reactivity and allergenic risks to home-made agricultural food in adult allergy patients. Objective: The aim of this study was to evaluate the IgE sensitization rate and cross-reactivity to home-made agricultural products and to establish a basic data for planning treatment and prevention methods of food allergy in adult allergy patients. Method: Five thousand three hundred forty allergy patients complaining of various allergic diseases and visiting the Allergy Clinic of Ajou University Hospital were enrolled. Skin prick tests were performed with home-made agricultural products including soybean, corn, buckwheat, chestnut, rye grain, potato, nut, wheatflour, carrot, onion and rice. If A/H ratio for each agricultural product showed more than 2+ on skin prick test, it was considered a positive response. ELISA inhibition tests were performed for cross-reactivity to agricultural products. Result: The sensitization rate to home-made agricultural products ranged from 2.2% to 9.5% in adult allergy patients. Sixty-five (3.8%) and sixty-four (3.7%) out of the one thousand seven hundred sixteen patients showed positive responses (A/H≥2+) to soybean and corn, positive responses to buckwheat and chestnut were noted in 84 (4.8%) and 66 (3.8%) patients respectively, followed by rye grain (9.5%), potato (5.7%), nut (5.4%), wheatflour (5.1%), carrot (5.1%), onion (3.3%) and rice (2.2%). Wheat flour and rye grain inhibited IgE binding to each other. Soybean, corn, Hop. J, wheatflour and rye grain extracts inhibited IgE binding to potato extracts in potato sensitized subjects sera. There was a strong inhibition between corn and soybean extract. Conclusion: The sensitization rate to major agricultural products ranges from 2.2% to 9.5% in adult allergy patients, and buckwheat and soybean were the most prevalent ones among the home-made agricultual products in this country. Agricultural products-specific IgE antibody that cross reacted with other agricultual products can cause allergic reaction in foods allergy. (Korean J Asthma Allergy Clin Immunol 2005;25:269-275)

성인 알레르기 환자에서 자연산과 유전자 변형 감자에 대한 감작률과 특이 IgE 항체 측정 및 알레르겐 성상 비교

윤성호 ( Sung Ho Yoon ),김현미 ( Hyun Mi Kim ),예영민 ( Young Min Ye ),강영미 ( Young Mi Kang ),서창희 ( Chang Hee Suh ),남동호 ( Dong Ho Nahm ),김승현 ( Seung Hyun Kim ),박해심 ( Hae Sim Park ) 대한내과학회 2005 대한내과학회지 Vol.69 No.6

목적 : 최근 유전자 변형 기술의 발달로 유전자 변형 음식의 생산과 소비가 급증하고 있는 추세이다. 본 연구에서는 국내에서 섭취가 많고 또한 유전자 변형 음식물 중 하나인 감자의 주요 알레르겐 성분을 규명하고 유전자 변형에 의한 알레르기 유해능을 평가하고자 하였다. 방법 : 각종 알레르기 질환으로 아주대학교 알레르기 클리닉을 방문한 15세 이상의 성인 1,886명을 대상으로 자연산 감자와 유전자 변형 감자 항원으로 피부단자시험을 실시하였고, 각각의 항원에 대해 면역효소법으로 혈청 내 특이 IgE 항체치를 측정하여 비교하였으며 면역효소억제시험으로 각 감자 항원에 대한 IgE의 특이성을 관찰하였다. 또한 특이 IgE 항체치가 높은 환자들의 혈청으로 IgE-Immunoblot 분석을 실시하여 감자내 주요 알레르겐을 규명하여 유전자 변형에 따른 차이가 있는지를 조사하였다. 결과 : 국내 성인에서 자연산과 유전자 변형 감자에 대한 감작률은 모두 5.7%이었으며, 특이 IgE 항체는 두 감자 항원간에 유사한 결과를 보였다. 또한 면역효소억제시험에서 강한 억제 양상을 보였으며 두 항원간에 알레르기 성상은 유사하였다. 각각의 감자에서 1개의 공통적인 주요 알레르겐(45 kDa) 성분을 규명하였으며 두 감자 항원간에 차이는 없었다. 결론 : 본 연구의 결과로 볼 때 감자의 경우 유전자 변형 공정에 따른 알레르기 유해성의 증가는 가능성이 낮을 것으로 생각한다. Background : The aim of this study was to evaluate the prevalence of IgE sensitization and allergic risk of genetically modified (GM) potato compared with wild one in adult patients with various allergic diseases. Methods : One thousand eight hundred eighty eight allergy patients visited Ajou University hospital and 38 healthy controls were enrolled. Skin prick tests were performed with wild and GM extracts. Phosphinothricin N-acetyltransferase (PAT) and neomycin phosphotransferase (NPT) gene was inserted in GM potato. Serum specific IgE level to the two potato extracts was measured by ELISA and their binding specificities were confirmed by ELISA inhibition test. IgE binding components in both wild and GM potato extracts were identified by SDS-PAGE and IgE-immunoblot. Results : One hundred eight patients (5.7%) showed positive responses (A/H ≥ 2+) on skin prick test to both wild and GM potatoes. Serum specific IgE was detectable in 50~88% among the positive reactors on skin prick test. ELISA inhibition tests showed similar inhibition pattern between wild and GM. Fourteen IgE binding components within wild potato and nine IgE binding components within GM potato with similar binding patterns, of which three major allergens in wild (26, 34, 45 kDa) and one (45 kDa) in GM one were noted. Conclusions : The sensitization rates to wild and GM potato extracts were 5.7% respectively, in adult allergy patients and one common major allergen (45 kDa) was identified. It is speculated that genetic manipulation of the potato did not increase allergenic risk.(Korean J Med 69:651-659, 2005)