Free Radical에 의한 노화 및 발암 기전

정해영 한국노화학회 1992 한국노화학회지 Vol.2 No.1

Free Radical Scavenging Activity of β-chitooligosaccharides

이승재,김은경,황진우,김세권,전병태,오현정,양송이,이승준,박표잠 한국키틴키토산학회 2009 한국키틴키토산학회지 Vol.14 No.4

The free radical scavenging activity of β-chitooligosaccharides (β-COSs) was evaluated by employing an electron spin resonance (ESR) spectrometer on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical. We prepared three kinds of β-COSs with different molecular weights: higher molecular weights (HMWβ-COSs: 50-10 kDa), medium molecular weights (MMWβ-COSs: 10-5 kDa), and lower molecular weight (LMWβ-COSs: below 5 kDa). The DPPH radical scavenging activity of HMWβ-COSs was the highest among the three kinds of β-COSs, and the IC50 value was 0.73 mg/mL. In addition, all β-COSs scavenged hydroxyl radical, and the IC50 values of LMWβ-COSs, MMWβ-COSs and HMWβ-COSs were 0.49, 0.62 and 0.47 mg/mL, respectively. Also, all β-COSs showed the alkyl radical scavenging activity, and the IC50 values of LMWβ-COSs, MMWβ-COSs and HMWβ-COSs were 0.35, 0.13 and 0.17 mg/mL, respectively. These results indicate that β-COSs possess antioxidative activity. The free radical scavenging activity of β-chitooligosaccharides (β-COSs) was evaluated by employing an electron spin resonance (ESR) spectrometer on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and alkyl radical. We prepared three kinds of β-COSs with different molecular weights: higher molecular weights (HMWβ-COSs: 50-10 kDa), medium molecular weights (MMWβ-COSs: 10-5 kDa), and lower molecular weight (LMWβ-COSs: below 5 kDa). The DPPH radical scavenging activity of HMWβ-COSs was the highest among the three kinds of β-COSs, and the IC50 value was 0.73 mg/mL. In addition, all β-COSs scavenged hydroxyl radical, and the IC50 values of LMWβ-COSs, MMWβ-COSs and HMWβ-COSs were 0.49, 0.62 and 0.47 mg/mL, respectively. Also, all β-COSs showed the alkyl radical scavenging activity, and the IC50 values of LMWβ-COSs, MMWβ-COSs and HMWβ-COSs were 0.35, 0.13 and 0.17 mg/mL, respectively. These results indicate that β-COSs possess antioxidative activity.

Effects of boron/nitrogen/phosphorus doping on the scavenging action of armchair single‑walled carbon nanotubes (armchair‑SWCNT) for OH radicals: a DFT study

Meenakshi Malakar,Pradeep Kumar Shukla 한국탄소학회 2023 Carbon Letters Vol.33 No.1

Hydroxyl radical (OH radical) is the most harmful free radical amongst the Reactive Oxygen Species (ROS) responsible for numerous diseases of DNA damage like mutagenesis, carcinogenesis and ageing. Therefore, it is important to find a suitable scavenger for OH radical. In the present contribution, we aim to investigate the ability of pristine armchair-SWCNT and B/N/P-doped armchair-SWCNT to scavenge OH radicals using DFT calculations. The calculations reveal that the B/Pdoped armchair-SWCNTs can act as a better scavenger for OH radical compared to pristine armchair-SWCNT but N-doped armchair-SWCNT does not act as a better scavenger for OH radical compared to pristine armchair-SWCNT. Furthermore, the developed scavenger is examined in terms of large-scale availability, biocompatibility, conductivity, stability and reactivity. For both in vivo and in vitro studies, the work is found to useful for enhancing SWCNT as a free radical scavenger.

An Analysis of Influencing Factors on Free Radicals among Women

Yoon Shin Lee,Mi Ryeong Song 보안공학연구지원센터 2016 International Journal of Bio-Science and Bio-Techn Vol.8 No.1

The goal of this study was to obtain baseline data for detoxification by analyzing the factors influencing free radical levelsin women. Data were collected through questionnaires and urine tests between November 2014 and February 2015 from women between 20 and 65 years of age. Data were analyzed using descriptive statistics, independent t-tests, Pearson’s correlation, and ANOVAusing SPSS version 21.0. Sleep satisfaction of participants was 55.4, fatigue was 61.3, and free radical was 646.9mmol/mL. Sleep satisfaction was significantly different according to economic status(F = 8.42, p<.001). Fatigue was significantly different according to health(F = 5.33, p = .006) and economic status(F = 8.42, p<.001). Free radicals were significantly different according to age(F = 8.84, p<.001), economic status(F = 10.12, p<.001), level of education(F = 6.79, p = .001), and occupation(F = 4.30, p = .006). Age(r = .437, p<.001), sleep(r = -.199, p = .013), and fatigue(r = .159, p = .049) were statistically significantly correlated with free radical levels. Factors influencing free radicals were age(β = .612, p<.001), medium economic status(β = -.324, p = .007), high economic status(β = -.467, p<.001), high school education(β = .338, p = .004), and college education(β = .338, p = .004); the explanatory power of all influencing factors was 33.2%. Detoxification management for reducing free radical levels has to consider subjects who are between the ages of 40 and 60 years and who have low economic status and level of education. A comprehensive analysis that includes free radical-related variables, such as diet, exercise, living habits, and environment, needs to be carried out in the future.

비만 범용 처방(處方)에 대한 탕전후(湯煎後) 경락 기능 진단기(QRIS)의 비만 연관 Code측정 및 수침후(水浸後) Free Radical 측정기를 이용한 측정에 대한 고찰

김재중,임형호,선유진,이동훈,Kim, Jae-Joong,Lim, Hyung-Ho,Sun, Yu-Jin,Lee, Dong-Hoon 척추신경추나의학회 2000 대한추나의학회지 Vol.1 No.1

Oryeong-San, Pangpungtongseong-San, Rangkyeoksanwha-Tang, Sipeemikwanjoong -Tang and Taeumjoui-Tang are not only examined using the code which are related to overweight in the QRIS but are also investigated the level of Free Radical using the Free Radical Measurement after steeping those 5 prescriptions in water. The results are as follows: 1. We indicated in the study of QRIS that 5 kinds of medicines which used most frequently In the treatment of the obesity appeared to affect the Immune system, spleen, kidneys, pancreas, the fatigue toxicity, TSH, and the metabolic disability but did not influence high on the contents of overweight and those of fatty cell, as well. in addition, there were no significant differences between the prescriptions as regards testosterone and progesterone. 2. In the Free Radical Measurement, Rangkyeoksanwha-Tang evaluated significantly high level of Free Radical, whereas others appear to have the similar level of Free Radical. These findings suggest that the treatment of the obesity affects particular body parts with respect to the control of overweight, although those medicines are not related directly to the areas(such as fatty cell Code), it is possible that they influence on the cure for the obesity. Furthermore, they indicate that with soaking prescription, Free Radical is not produced as much as we expected.

비용에서 스테로이드 투여 후 활성산소의 생성과 항산화효소 활성도의 변화

조진학,김현웅,김희동,오원희,정인교,정근,임현준 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.1

활성산소에 의한 피부노화와 항산화비타민의 효능에 대한 이론적 고찰

홍재기 한국피부과학연구원 2009 대한피부미용학회지 Vol.7 No.2

피부노화를 설명하기에 적합한 노화이론은 환경기인설(environmental etiology)이다. 그 중 자외선과 호흡을 통해 생성되는 활성산소(free-radical)는 피부노화에 가장 중요한 요인으로 간주되고 있다. 이렇듯 활성산소가 피부노화의 주요원인으로 밝혀짐에 따라 활성산소를 제거하는 항산화제, 즉 항산화비타민의 체내흡수가 피부노화를 예방하고 지연시키는 데에 중요한 연구과제가 되고 있다. 하지만 피부미용학적 연구에서는 피부노화를 예방하고 지연시키는 측면이 화장품을 통해 항산화비타민을 흡수케 하거나 매뉴얼테크닉을 이용한 외양적인 변화에만 목적을 두고 있는 실정이다. 유용성을 가지고 있는 화장품과 매뉴얼테크닉은 피부미용학에서 필요한 항목이다. 그러나 피부노화를 예방하고 지연시키는 측면이 화장품과 매뉴얼테크닉으로만 해결하려 한다면 곧이어 한계에 부딪치게 될 것이다. 피부노화란 본문에서처럼 생체 산화에 따른 외부변화현상이기 때문이다. 따라서 피부의 아름다움을 유지하고 관리하는 피부미용학적 연구에서도 피부의 외양적 관리와 함께 항산화비타민의 체내흡수도 병행되어야 할 것인데, 그러기 위해서는 항산화비타민의 체내흡수를 통한 피부의 변화양상에 대한 지속적인 연구가 이루어져야 할 것이다. 이러한 연구가 이루어질 때에 피부미용학의 범위가 넓어질 것이며, 고객에 대한 전인적 관리도 기대할 수 있을 것이다. A theory of aging proper for explaining skin aging is environmental etiology. Out of them, the free-radical, which is generated through ultraviolet radiation and breathing, is being regarded as the most important factor to skin aging. Free-radical leads to damaging skin cell and tissue, thereby resulting in speeding up skin aging that is characterized by reduction in elasticity, wrinkling, and revelation in liver spots & freckles. A method, which can prevent and delay this skin aging, is to inhibit free-radical or make antioxidant system strong. However, what inhibits free-radical, which is generated by breathing or external environment, is unreasonable. Accordingly, a method of making antioxidant system strong needs to be sought. In our body, the antioxidant vitamins(vitamin C, vitamin E, β-carotene) available for a supply from the outside to the inside of the body, are forming antioxidant system along with its enzymes(enzymes such as SOD, CAT, GPX). These antioxidant vitamins are available for increasing quantity, thereby being able to allow antioxidant system in a living body to be strong by taking it. In addition, the antioxidant vitamins remove free-radical inside and outside of cell membrane, thereby having effect on aesthetics of inhibiting damage in collagen and elastic fiber and of preventing pigmentation and wrinkling in the skin. For this reason, the absorption in the body of antioxidant vitamins is becoming an important research task in preventing and putting off skin aging. Accordingly, in the aspect of preventing and delaying skin aging even in an aesthetics research of keeping and managing beauty in the skin, the customer management will need to be developed in line with it along with an extensive research of seeking for solution by grasping a fundamental cause for a change in the skin.

홍화자 분획물이 사염화탄소 유발 간손상 흰쥐에서 지질과산화와 oxygen free radical 제거 효소 활성도에 미치는 영향

정기화,정춘식,정정숙 德成女子大學校 藥學硏究所 1999 藥學論文誌 Vol.10 No.1

저자들은 사염화탄소로 유발된 간손상에 대한 홍화자 메탄올 추출물과 이를 계통 분획한 분획물의 보호 효과를 이미 보고하였다. 본 실험에서는 홍화자 분획물의 간보호 작용에 대한 기전을 살펴보기 위하여 지질과산화와 oxygen free radical 제거 효소 활성을 측정하였고 조직병리학적 분석을 통하여 간보호 효과를 확인하였다. 홍화자 BS-5 분획물은 CCl_4로 간손상을 유발시킨 흰쥐에서 superoxide dismutase의 활성도를 유의적으로 증가시켰으며, in vitro에서의 1, 1-diphenyl -2-picrylhydrazyl radical를 직접적으로 제거하였다. 또한 조직병리학적 분석에서도 홍화자 BS-5분획투여군은 CCl_4 투여로 인한 중심정맥 주변의 ballooning degeneration, 염증 세포 침윤 및 괴사를 완화시켜 간손상 보호 작용을 나타내었으며, 이는 free radical scavenging 작용에 의한 것으로 생각된다. Previous studies have shown that methanol extract and its butanol fraction of Carthamus tinctorius L. Semen have the hepatoprotective effect on the CCl_4-induced hepatotoxicity. The hepatoprotective effect fo the subfractions of butanol fraction has been evaluated by analyzing oxygen free radical scavenging enzyme activities and histopathological examinations. In BS-5 subfraction treated group, the activity of superoxide dismutase has been significantly increased as compared with that of CCl_4 treated rats. Antioxidant activity has been evaluated by the examination of the scavenging ativity against 1,1-diphenyl-2-picrylhydrazyl radical. BS-5 subfraction has shown strong antioxidant activities. The histopathological examination showed that the treatment of BS-5 subfraction has relieved the ballooning degeneration of hepatocytes which had been generated by CCl_4. It appears that the protective effect of BS-5 subfraction would be mediated of the attenuation of lipid peroxidation by acting as a free radical scavenger. which were based on the increase of superoxide dismutase activity.

Free Radical Scavenging Activity of Enzymatic Hydrolyzates of Hot Water Extract from the Shell of Reeve's Turtle (Chinemys reevesii)

Je, Jae-Young,Kim, Eun-Kyung,Park, Pyo-Jam,Kang, Mi-Kyung,Ahn, Chang-Bum The Korean Society of Fisheries and Aquatic Scienc 2008 Fisheries and Aquatic Sciences Vol.11 No.2

The shell of Reeve's turtle has been used as a traditional folk medicine in Korea. We produced a hot water extract from Reeve's turtle shell according to the traditional medical practice. To release bioactive peptides, the hot water extract was enzymatically hydrolyzed with various proteases, and the free radical scavenging activity of the hydrolysate was investigated against 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl and peroxyl radicals. The free radical scavenging activity of the enzymatic hydrolysates varied from 1 to 79% depending on the enzymes, free radical species, and concentration. The $EC_{50}$ values demonstrated that the enzymatic hydrolysates of hot water extract from the shell of Reeve's turtle are potential antioxidants.

Free Radical Scavenging Activity of the Seed of Phaseolus calcaratus Roxburgh

Minghao Fang,Hyoung-Kwon Cho,Yun-Pyo Ahn,Sang-Jeong Ro,전영미,Wan Kyuun Whang,이정채 한국생약학회 2010 Natural Product Sciences Vol.16 No.3

The seed of Phaseolus calcaratus Roxburgh (PHCR) is traditionally used for anti-pyretic and anti-inflammatory effects. Although these effects are believed to be related to its antioxidant potential, little information is available for the mechanisms by which PHCR seed might scavenge free radicals or otherwise act as an antioxidant. In the present study, we purified some fractions from the ethanol extract of PHCR seed and evaluated each fraction's ability to scavenge free radicals generated by cell-free systems. We also identified active compound that is putatively responsible for free radical scavenging by analyzing NMR spectra. PHCR samples exhibited a concentration-dependent radical scavenging activity against hydroxyl radicals, superoxide anions, and DPPH radicals. Of the samples tested, a methanol-eluted sub-fraction from the PHCR extract, named FF4, scavenged these radicals more effectively than the other fractions. We identified catechin-7-O-b-D-glucopyranoside as the active compound responsible for free radical scavenging potential of FF4.