흡수성 차폐막에 배양된 구개관세포의 증식양상의 비교

이창훈,이만섭,권영혁,박준봉,허익,Lee, Chang-Hoon,Lee, Man-Sup,Kwon, Young-Hyuk,Park, Joon-Bong,Herr, Yeek 대한치주과학회 2003 Journal of Periodontal & Implant Science Vol.33 No.2

The purpose of this study is to evaluate the phenomenon of attachment and spreading of the cultured rat calvarial cell inoculated on their surface of different kinds of biodegradable membrane which had been used on tissue regeneration on periodontal defects by using scanning electron microscope. In this experiment 30 Sprague-Dawley male rats (mean BW 150gm) were used to harvest abundant number of cell in the short period. The rats were sacrificed by decapitatioan to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Biodegradable barrier membrane were collected with collagen type, and were divided into 3 different kind of surface such as scattered, polarized and fine-net type as their surface texture. Microcover plate which usually used for cell culture was used as control for smooth surface. All the membrane were seeded with cultured calvarial cell on their surface. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. After the culture as designed time, all the membrane were washed with 0.1 M Phosphate Buffered saline and fuxed with 2.5% Glutaraldehyde. And all specimen were treated with $OsO_4$, and Tannic acid before drying the cell for coating the cell with gold. Scanning Electron Microscope was used to observation. The following results were obtained. I. During the whole period of experiment, the phenomenon of cell attachment and spreading were revealed similar pattern to compare with smooth surface culture plate and ordinary culture dish. 2. The shape of cell attachment and spreading on the surface of barrier membrane were observed no remarked difference pattern between smooth surface culture plate and ordinary culture dish. 3. The cytoplasmic process of cultured calvaria cell extent to the deep portion of barrier membrane like as their own proper shape. 4. There were no remarkable relationships between the degree of cultured cell spreading and surface structure of barrier membrane. 5. Slight starified layer of cultured calvaria cell were observed on the scattered type of resorbable membrane, Conclusively, this study thus suggest that cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of carrier for many cell which could be used as new tissue regeneration, and those tissue engeering technique may become an new method in the approach to the repair of bone defects.

1930년대 소비에뜨 문화의 패러다임으로서 ‘문화성’

오원교(Oh Won Kyo) 한국노어노문학회 2009 노어노문학 Vol.21 No.1

본 연구는 1930년대 소비에뜨 러시아에서 문화성의 개념적 의미와 역사적 맥락, 내외적 양상과 진화의 역학, 그리고 그것에 대한 성격 규정과 역사적 평가 등에 대한 탐구이다. 러시아의 역사에서 1917년 혁명은 정치-사회적 차원에서 중대한 전환점이자, 문화적 차원에서도 새로운 시대의 도래로서 기록된다. 새로운 사회의 건설과 함께, 특히 새로운 인간의 창조라는 혁명의 과제는 문화의 몫이었고, 그것의 실행은 일종의 문화 혁명이었다. 소비에뜨 러시아에서 문화적 변동은 사회주의 체제가 종말을 고할 때까지 멈추지 않았지만, 문화 혁명에 값하는 근본적인 변화는 스딸린시대, 특히 1930년대에 가장 뚜렷하게 나타났다. 바로 이 시기에 문화 영역의 전면에 등장한 것이 문화성이다. 문화성은 일상, 환경, 의식, 행위를 포괄하는 1930년대 소비에뜨 일상 문화의 패러다임으로서, 스딸린주의 문화의 핵심적 요소였다. 문화성은 국가가 새로운 소비에뜨적 시민을 육성하기 위한 문화적 가치 체계이면서, 동시에 개인들이 주체적으로 전유하고자 했던 문화적 규범 체계였다. 말하자면 그것은 소비에뜨적 유형의 근대적 자아 만들기의 매트릭스에 다름 아니었다. 또한 문화성은 개인에 대한 배제의 전략이었던 테러와는 달리 대중에 대한 포용의 전략으로서, 강제성 보다는 자발성에 기초하는 유연한 일상의 정치학이었다. 하지만 역사적 현실이 말해주듯이, 문화성을 통한 소비에뜨적 주체의 형성은 평등주의라는 이상의 실현이 아니라, 새로운 지배 계층의 출현을 야기했고, 그것은 그들만의 문화적 패러다임으로 특권화 되기도 했다. 문화성이 결박된 이러한 모순은 문화성 개념의 역사적 진화속에서 잘 드러난다. 하지만 문화성의 체현자들이 스딸린 시대뿐만 아니라 그 후의 소비에뜨 역사에서 주도적인 역할을 지속적으로 수행했던 것처럼, 소비에뜨 역사 속에서 문화성의 역학은 그것의 소멸이 아니라 오히려 생명력을 증거하고 있다. 따라서 그것에 대한 역사적 평가는 단순한 긍정과 부정이 아니라 냉정한 객관화의 문제이다. This paper is a study on culturedness(kulturnost') of the Soviet Russia in the 1930s; on its conceptual meanings, historical contexts, inner/outer aspects, dynamics of its evolution, characterization and historical assessments. In the history of Russia, the 1917 Revolution is recorded not only as a critical juncture for Russian politics and society, but also as a turning point into a new cultural era. The revolutionary mission of 'constructing a new society,' especially that of 'creating a new type of human being,' was assigned to culture, and putting it in action meant a kind of cultural revolution. While cultural alterations in Soviet Russia had not stopped until the end of communist system, fundamental changes corresponding to a cultural revolution appeared most clearly in the Stalin era, especially in the 1930s. It was the notion of culturedness that emerged at the forefront of cultural sphere in this period. Culturedness was the key element of Stalinist culture as a paradigm of Soviet daily culture containing everyday life, environment, consciousness, and behaviors in the 1930s. It was a cultural value system created by the state to foster new Soviet citizens and, at the same time, a system of cultural norms that the individuals tried to possess independently. In other words, culturedness was no other than a matrix to cast a modern subject of Soviet type. On the while, culturedness was a kind of flexible politics on daily life based on self-motivation rather than coercion, because it was a strategy to embrace the mass of people, not a terror as a strategy to exclude the individuals. As the historical realties tell us, however, formulating Soviet subjects through the culturedness did not result in realization of the ideal of egalitarianism, but rather caused the appearance of new ruling classes and increasingly degenerated into just their own exclusive cultural paradigm. This paradox of culturedness can be found evidently in the historical evolution of the concepts of culturedness. As those who embodied culturedness had continued to play the leading roles not only in the Stalin era but also in the subsequent Soviet history, however, the dynamics of culturedness throughout the Soviet history proves more of its vitality than its extinction. In this regard, this paper argues that historical assessments on the culturedness of the Soviet Russia in the 1930s should not be a matter of confirmation or negation, but of sober and sincere objectification.

배양된 정상 갑상선세포에서 LECTIN 에 의한 HLA - DR 항원 발현에 관한 연구

박성기(Sung Ki Park),이명식(Myung Shik Lee),조보연(Bo Youn Cho),고창순(Chang Soon Koh),이문호(Mun Ho Lee),차창룡(Chang Young Cha),황응수(Eung Soo Hwang),오승근(Seung Keun Oh) 대한내과학회 1988 대한내과학회지 Vol.35 No.5

N/A Although it has been suggested that HLA-DR Ag expression in thyrocytes by nonspecific immune stimuli such as lectin may play an important pathogenetic role in autoimmune thyroid diseases, its precise mechanism has not been established. The purpose of this study is to investigate a part of the mechanism and features of lectin-induced expression of HLA-DR antigenicity in cultured normal thyrocytes. By an indirect immunofluorescence method using monoclonal anti-HLA-DR Ab, this study was performed to assess the effects of various lectins, as well as the effects of culture duration, cyclosporine, prostaglandin E₂, and gamma-interferon on HLA-DR Ag expression in cultured normal thyrocytes, and also to compare cytoplasmic and surface expression of HLA-DR Ag. The results were summarized as follows: 1) In 5 separate experiments with normal thyrocytes cultured with 10 mg/ml leucoagglutinin, HLA-DR Ag was detected on the 2nd day of culture. The expression rate increased to a maximum on the 7th to 9th day of culture and decreased thereafter. 2) The positivity of HLA-DR Ag expression by T-cell specific mitogenic lectins was 60% in the cultured cells with 2 mg/ml phytohemagglutinin, 60% in those with 10 mg/ml concanavalin A, and 50% in those with 10 mg/ml leucoagglutinin on the 7th day of culture, There were no significant changes of HLA-DR Ag expression in higher concentrations. HLA-DR Ag was expressed in 40% of cultured thyrocytes with 100 mg/ml pokeweed mitogen but HLA- DR Ag expression was not induced by lipopolysaccharide, which is not a T-cell mitogenic lectin. 3) Leucoagglutinin-induced expression of HLA-DR Ag was reduced by cyclosporine which suppresses secretion of lymphokines from T-lymphocyte, but not by prostaglandin E₂, which suppresses HLA-DR Ag expression in macrophages. 4) Gamma-interferon-induced expression of HLA-DR Ag was not reduced by cyclosporine. 5) HLA-DR Ag expression was not induced by hormones such as TSH, insulin and hydrocortisone. 6) In normal thyrocytes cultured with concanavalin A or leucoagglutinin, the HLA-DR Ag expression rates in the cytoplasm and cell surface were nearly similar. These results, that HLA-DR Ag expression was induced by T-cell mitogenic lectin but suppressed by cyclosporine (inhibitor of gamma-interferon synthesis in T-lymphocyte) and that the expression rates in the cytoplasm and cell surface were nearly similar suggest that the T-lymphocyte is involved in lectin-induced expression of I-ILA-DR Ag in cultured normal thyrocytes and that HLA-DR Ag is produced in the cytoplasm of thyrocytes.

PUVA가 배양 각질형성세포의 성장과 배양 멜라닌세포의 성장 , 멜라닌양 , 수지상돌기 수 및 길이에 미치는 영향

전일선 ( Il Sun Jun ),박재경 ( Jae Kyung Park ),허충림 ( Choong Rim Haw ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.5

Background : The combination of psoralen and UVA (PUVA) as a model of photochemotherap, has been used in a wicle variety of cutaneous disorders such as psoriasis, vitiligo, mycosis fungoides and atopic dermatitis. The mechanism of PUVA of psoriasis is based on the fact. that PUVA causes photoconjugation of psoralens to DNA and a subsequent suppression of mitosis, DNA synthesis, and keratinocyte proliferation. Although PUVA apparently inhibits keratinocytie proliferation and is effective therefore in the treatment of psoriasis, PUVA increases pigmentations by stimulating melanocyte proliferation and melanin synthesis in vivo. Objective : We tried to investigate the PUVA effects on the proliferation and different,iation in cultured human keratinoc tes and melanocytes. Methods : We examined morphologic changes, and the number of the cultured human keratinocytes and melanoiytes and melanin contents in a control group and experimental group. (UVA group, 8-MOP group and PUVA group). i.e., UVA group was exposed to UVA at 60mJ, of. 8-MOP group was acJded at dose of 2 x 10 M to medium for 30 minutes. PUVA group was exposed to UVA at 60mJ, of after adding in 8 MOP at Zx 10 M for 30 minutes. Results : 1. Morphologic changes of cultured human keratinocytes and melanocytes. There were no significent changes between the control group and the experimental groups in keratinocytes and melanocytes after 24, 48 and 72 hours culture. The number and length of meianocyte dendrites showecl no significant differences between the groups after 24, 48 and 72 hours culture(p.>0.05). 2. Proliferation of cultured human keratinocytes and melanocytes 1) The number of keratinocytes in 8-MOP and PUVA groups decreased significantly more than in the control and LVA groups at 72nd hour after culture (p<0.01). 2) The number of melnocytes showed no differences between the groups at 72nd hour after culture (p>0.05). 3. Melanin contents in iultured human melanocytes The melanin contents increased significantly in the PUVA groups compared to that in the other groups at 72nd hour after culture (p<0.01). Conclusion : In culturel human keratinocytes, PUVA has no effect on the morphology and differentiation, hut inhibit proliferation. In cultured human melanocytes, PUVA has no effect on morphology and proliferation, but it increases the melanin contents. (Kor J Dermatol 1995;33(5): 886-894)

배양된 이자섬 $\beta$세포의 미세구조적 변화와 인슐린 분포 양상

민병훈,김수진,Min, Byoung-Hoon,Kim, Soo-Jin 한국현미경학회 2007 Applied microscopy Vol.37 No.4

이자섬은 이자를 구성하는 외분비조직에 둘러싸여 존재하는 내분비세포의 집단으로, 이자섬에서 분비되는 인슐린은 $\beta$세포에서 분비되는 호르몬이며, 세포질의 리보좀에서 합성되고 골지체를 경유하여 세포질로 방출되는 기작을 가지고 있다. 충분한 양의 이자섬 이식은 인슐린 의존형 당뇨병인 제1형 당뇨병에서 정상혈당을 회복시키고, 당뇨 합병증을 방지할 수 있는 치료방법으로 사용되고 있다. 하지만 당뇨병 환자에게 이식을 위한 이자섬의 양에 비해 공여자로부터 증여된 이자섬의 양은 제한적이다. 이러한 문제점은 이자섬의 증식으로 연구되고 있으나, 배양된 이자섬이 정상 조직내의 이자섬과 형태적 기능적으로 동일한 것인지에 관한 연구는 미비하였다. 따라서 본 연구에서는 분리된 이자섬과 배양된 이자섬을 구성하는 세포들의 내부구조의 변화를 주사전자현미경, 투과전자현미경을 이용하여 세포의 미세구조를 확인하고, 인슐린 항체를 이용한 $\beta$세포 내의 인슐린 분포양상을 확인하여 다음과 같은 결과를 얻었다. 분리된 이자섬의 $\beta$세포는 일반적인 핵 미토콘드리아, 세포질세망 그리고 인슐린 과립이 분포하고, 배양된 이자섬 $\beta$세포의 경우 분리된 이자섬에 비하여 일반적인 핵의 모습과 부피가 증가한 세포질과 미토콘드리아, 세포질세망 그리고 골지체의 발달이 이루어지는 것으로 관찰되었다. 인슐린 과립의 경우 분리된 이자섬에 비해 감소하며, 세포막 주위에 분포하는 것으로 관찰되었다. 배양된 이자섬에서 관찰되는 인슐린 과립 분포의 변화, 세포질세망의 증가, 골지체의 발달은 배양된 이자섬 $\beta$세포의 인슐린 생성 분비 기능의 향상과 부피의 증가가 이루어지기 위한 세포 내부의 형태적 변화가 이루어지는 것으로 추측된다. The Pancreatic islet are the clusters of endocrine cells scattered through out the exocrine pancreas. Transplantation of a sufficient pancreatic islets can normalize blood glucose level so that may prevent devastating complications of type I diabetes(IDDM) and other side effects of the IDDM. Recently, there are several approaches to transplant sufficient pancreatic islet, and it was comprised in increase or regeneration of the endogenous $\beta$-cell mass from donor's pancreas, but relatively few studies have been devoted to the morphological characters of the isolated and 3 day cultured pancreatic islets. We investigated morphological pattern of intracellular structure of isolated and 3 day cultured pancreatic islets. The morphological characters of the pancreatic islets were observed by scanning electron microscope and transmission electron microscope, and insulin distribution of the each islets were observed by transmission electron microscope, and were labeled with insulin antibody. Intracellular structures including nuclei, mitochondria, RER, Golgi complex and many secretory granules were normally appeared in the isolated pancreatic islets which was extracted immediately dornor's pancreas, however, There is a significant morphological changes between the 3 day cultured pancreatic islets and isolated islets. 3 day cultured pancreatic islet's $\beta$-cells had normal nuclei but increased cytoplasm mass and RER and developed Golgi complex. Insulin secretory granules were decreased in numbers rather than isolated pancreatic islet. In this study, the pattern of intracellular structure variation was examined during pancreatic islet culture. Most distinct features are variation of the insulin secretory granules, and developed RER, and dilated golgi complex. Therefore, we suggested that the various change of the morphological characters on cultured pancreatic islets were responsible for the function(biosynthesis and secretion of insulin) and growth. These results were also cultured islets have greater ability to recover and maintain normoglycemia than isolated islet transplantation.

α - MSH가 배양 정상 인체 멜라닌세포의 형태 , 생존 및 멜라닌화에 미치는 영향

김형섭 ( Hyoung Seob Kim ),이무형 ( Mu Hyoung Lee ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.2

Background: The effects of melanocyte stimulating hormone(MSH) on the integument of many species, including mammals, are well known. The significance of MSH as a physiological regulator of cutaneous pigmentation in humans is still controversial. Although the administration of MSH results in skin darkening, previous reports suggest that cultured human melanocytes are relatively unresponsive to this peptide. This may be related to the conditions under which the melanocytes were cultured. Objective: The purpose of this study was to investigate the effect of a -MSH on the morphological changes, survival, and melanization of cultured human melanocytes in a basal medium without any mitogen. Method: We examined the morphological changes, number and melanin contents of cultured human melanocytes in control(absence of a-MSH) and experimental groups(presence of 10-8 M, 10-7 M, and 10-6 M a -MSH). Results : 1. There were no significant morphological changes of cells between the control and experimental groups after 24, 48, and 72 hours' culture. The number and length of melanocyte dendrites showed no significant difference between the groups after 24, 48, and 72 hours' culture. 2. The number of melanocytes in the experimental groups(presence of 10-7 M, and 10-6 M a -MSH) were significantly higher than the number of melanocytes in control group after 72 hours culture(p<0.05). This effect of a-MSH was dose-related. 3. The melanin contents slightly increased in the experimental groups. The significant difference between the groups was showed in the presence of 10-8 M a-MSH. Conclusions : a -MSH has no effect on the morphology, but increases the survival of cultured human melanocytes and has a melanogenic effect. (Korean J Dermatol 1998;36(2): 280-285)

Genetic Similarity and Variation in the Cultured and Wild Crucian Carp (Carassius carassius) Estimated with Random Amplified Polymorphic DNA

Yoon, Jong-Man,Park, Hong-Yang Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.4

Random amplified polymorphic DNA (RAPD) analysis based on numerous polymorphic bands have been used to investigate genetic similarity and diversity among and within two cultured and wild populations represented by the species crucian carp (Carassius carassius). From RAPD analysis using five primers, a total of 442 polymorphic bands were obtained in the two populations and 273 were found to be specific to a wild population. 169 polymorphic bands were also produced in wild and cultured population. According to RAPD-based estimates, the average number of polymorphic bands in the wild population was approximately 1.5 times as diverse as that in cultured. The average number of polymorphic bands in each population was found to be different and was higher in the wild than in the cultured population. Comparison of banding patterns in the cultured and wild populations revealed substantial differences supporting a previous assessment that the populations may have been subjected to a long period of geographical isolation from each other. The values in wild population altered from 0.21 to 0.51 as calculated by bandsharing analysis. Also, the average level of bandsharing values was $0.40{\pm}0.05 $ in the wild population, compared to $0.69{\pm}0.08$ in the cultured. With reference to bandsharing values and banding patterns, the wild population was considerably more diverse than the cultured. Knowledge of the genetic diversity of crucian carp could help in formulating more effective strategies for managing this aquacultural fish species and also in evaluating the potential genetic effects induced by hatchery operations.

人蔘이 산소유리기로 손상된 척수신경세포의 손상에 미치는 영향

田炳勳,文炳淳,姜益賢 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.1

산소자유기는 중풍인 뇌졸중을 비롯하여 뇌허혈, 파킨슨씨병과 같은 뇌질환의 병리적 요인으로 밝혀짐에 따라 이의 독성효과나 작용현상에 대한 기전을 규명하기 위하여 많은 연구가 진행되어 왔다. 그러나 산소자유기의 독성효과에 대하여 아직까지 이에 대한 자세한 기전이 밝혀져 있지 않고 치료방법 역시 미흡한 실정이다. 본 연구는 산소자유기의 신경독성 효과에 대한 기전을 규명하기 위하여 배양소의 희소돌기아교세포에 여러 농도의 산소자유기를 처리한 다음 산소자유기의 독성효과를 측정하였으며 또한 산소자유기의 독성효과에 대한 인삼추출물의 영향을 MTT assay법으로 조사하여 다음과 같은 결론을 얻었다. xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 인삼추출물을 배양 희소돌기아교세포에 전처리한 결과 XO/HX의 독성효과를 유의성있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 인삼추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. To elucidate the neurotoxic mechanism of oxygen radicals in cultured bovine oligodendrocytes, cytotoxic effect was measured by MTT assay after cultured oligodendrocytes were grown in the medium containing various concentrations of oxygen radicals. In addition, neuroprotective effect of herb extract, Korean red Ginseng was examined by MTT assay in cultured oligodendrocytes. Cell viability of cultured oligodendrocytes was remarkably decreased by xanthine oxidase(XO) and hypoxanthine(HX) in dose-dependent manner, and Korean red Ginseng protected remarkably oxidant-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured bovine oligodendrocytes, and herb extracts such as Korean red Ginseng are effective in blocking the neurotoxicity induced by oxygen radicals in cultured bovine oligodendrocytes.

신경세포성장인자로서 척수운동신경세포의 손상에 미치는 한약재의 약류별 효능 및 기전에 관한 연구

鄭遇悅,朴承澤,成彊慶,李星根 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

산소자유기가 흰쥐의 배양 운동신경세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약재중 보혈약류와 보기약류의 대표적인 처방인 사군자탕과 사물탕의 신경세포독성의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 사물탕과 사군자탕이 XO/HX의 독성효과를 유의있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 사군자탕과 사물탕등의 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. In order to elucidate the neuroprotective effect of Sagunjatang and Samultang on oxidant-induced neurotoxicity in cultured rat spinal motor neurons, the colorimetric assay such as MTT assay was performed and light microscopic study was also carried out after cultured spinal motor neurons from neonatal rat were treated with the medium containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, protective effect of herb extracts on the neurotoxicity induced by oxygen radicals was examined in these cultures. The results were as follows : Cell viability of cultured rat spinal motor neurons which were exposed to various concentrations of 30mU/ml XO and 0.1mM HX for 3 hours, was decreased in a dose-dependent manner. MTT50 value was 30mU/ml XO and 0.1mM HX after motor neurons were incubated with various concentrations of XO/HX for 3 hours. Cultured spinal motor neurons showed degenerative changes such as the decrease of cell number and loss of cell processes when neurons were cultured in the medium containing 30mU/ml X0 and 0.1mM HX for 3 hours. Oxygen radicals were toxic on cultured rat spinal motor neurons by the decrease of cell viability. Herb extracts, Sagunjatang and Samultang prevented the oxidant-induced neurotoxicity significantly after spinal motor neurons were preincubated with herb extractions for 2 hours before exposure of oxygen radicals. These results suggest that oxygen radicals have neurotoxic effect on cultured spinal motor neurons derived from neonatal rat, and selective herb extracts such as Sagunjatang and Samultang are very effective in protecting the neurotoxicity induced by oxygen radicals.

배양 희소돌기아교세포에 있어서 산소자유기의 神經毒性에 대한 淫羊藿의 효과

朴炳林,田炳薰,朴承澤 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.2

산소자유기가 배양 소의 희소돌기아교세포에 미치는 세포독성에 대한 기전을 규명하기 위하여 여러 농도의 산소자유기를 배양 신경세포에 처리한 후 산소자유기의 신경독성 효과를 분석하였으며 또한 산소자유기에 의하여 유발된 신경독성에 대한 한약추출물인 淫羊藿(Epimedium Koreanum Nakai)의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. Xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 또한 음양곽이 XO/HX의 독성효과를 유의게 방어하였다. 이상의 결과로 부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 음양곽과 같은 한약추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. To elucidate the neurotoxic mechanism of oxygen radicals in cultured bovine oligodendrocytes, cytotoxic effect was measured by MTT assay after cultured oligodendrocytes were grown in the medium containing various concentrations of oxygen radicals. In addition, neuroprotective effect of herb extract, Epimedium Koreanum Nakai was examined by MTT assay in cultured oligodendrocytes. Cell viability of cultured oligodendrocytes was remarkably decreased by xanthine oxidase(XO) and hypoxanthine(HX) in dose-dependent manner, and Epimedium Koreanum Nakai protected remarkably oxidant-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured bovine oligodendrocytes, and herb extracts such as Epimedium Koreanum Nakai are effective in blocking the neurotoxicity induced by oxygen radicals in cultured bovine oligodendrocytes.