건선환자의 말초혈액내 면역세포 ( T Cell , T Subsets 및 B Cell ) 의 정량과 광화학요법 ( PUVA ) 이 이들 면역세포에 미치는 영향에 관한 연구

허충림(Choong Rim Haw),정영철(Young Chul Jung),한을남(Eul Nam Han),임수덕(Soo Duk Lim),명기범(Ki Bum Myung),국홍일(Hong Il Kook) 대한피부과학회 1983 大韓皮膚科學會誌 Vol.21 No.5

Recent studies have demonstrated that the patients with psoriasis have various immune alternation in both humoral and cellular immunities. However the results of these are still controversial. These facts promoted us to analyze the immune cells (T cell, T subsets and B cell) by rosette method for these cells in peripheral blood of 15 patients with psoriasis. And we also analyzed the effects of photo- chemotherapy(PUVA) on these cells. The results were summerized as follows; 1. The mean values of T cell, T, T, cell and B cell in peripheral blood of the- patients with psoriasis before PUVA therapy were 56. 2+-5. 7%, 35. 1+-6.9%, 5. 5+l. 4%, 11. 6+4. 0% respectively, These results of immune cells showed signi- ficantly lower mean values when these were compaired with the mean values. of nomnal control group(T cell; 65. 2+-2. 9%, TM cell; 45. 8+-3. 2%, T cell; 7. 3+ 1. 3% B cell; 16. 2+2. 4%), respectively (T cell, Tm Tg, p <0. 01, B cell; p <0. 05). 2. The mean values of T cell, T, T, cell and B cell in peripheral blood -countinue-

특집 : 피부 알레르기 ( Special Edition : Skin Allergy )

허충림(Choong Rim Haw) 경희대학교 경희의료원 1996 慶熙醫學 Vol.12 No.2

Retinoids가각질형성세포에서 감마인터페론에 의한 HLA - DR , ICAM - 1 발현에 미치는 영향

이춘봉(Choong Bong Lee),심우영(Woo Young Sim),박재경(Jai Kyung Park),허충림(Choong Rim Haw) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.1

Background : Retinoids exert a wide range of effects on cell growth and development and have important effects on keratinocytes differentiation in vvc and in vitro. Besides the effects on epithelial differeotiation, modulation of cellular and amoral responses of lymphocytes, changes in natural killer and T-killer cell activities and riodulation of antigen presenting properties were shown by retionds. Objective : We studied to investigate the immunologic role of etinoids. Methods : With foreskin. the effects of 13-cis retinoic acid and etretinate on interferony induced HLA-DR and ICAM-1 expression of kratinocytes were!ev luated. Results : 1. When keratinocytes were grown in low calcium media, priliferation was inhibited only with 8 x 10 M of 13-cis retinoic acid. 8 x 10 , 8 x 10 M of 13-cis retinoic acid and 8 x 10 , 8 x 10, 8 x 10 M of etretinate had no effect on keratinocytes roiferation. When cultured in 0.15 mM calcium media or 1.0 mM calcium media, 13-cis retino acid and etretinate had no effection keratinocytes proliferation. 2. Keratinocytes HLA-DR expression was decreased with 8 x 10 , 8 x 10 M of 13-cis retinoic acid in 0.15 mM carcium media and 8 x 10 , 8 x 10 M of 13-cis retinoic acid in 1.0 mM calcium media. Etretinate had no effect on keratinocytes HLA-DR expression. 3. Keratinocytes ICAM- 1 expression was increased with 8 x 10 M of 13-cis retinoic acid in low calcium. media. When cultured on 0.15 mM carcium media, ICAM-1 expression was increased with 8 x 10 M of 13-cis retinoic acid and tetinate. When cultured in 1.0 mM calcium media, ICAM-1 expression was increased with 8 x 10, 8 x 10 , 8 x 10 M of 13-cis retinoic acid and 8 x 10, 8 x 10 , 8 x 10 M of etretinate. Conclusion : These results suggest that retiniods have an immunomodulating effect as well as effects on epithelial differentiation. Clarification of the mechanism of increased expression of ICAM-1 and decreased expression of HLA-DR remains to the proved. (Kor J Dermatol 1995; 33(1): 33-43)

제 1형 Trichorhinophalangeal 증후군

김형섭,심우영,허충림 ( Hyoung Seob Kim,Woo Young Sim,Choong Rim Haw ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Trichorhinophalangeal syndrome(TRPS) was first described in 1966 by Giedion. It is a rare genetic disease and divided into two groups : TRPS type I and TRPS type II. TRPS type I is an autosomal dominant inherited disorder, defined by three characteristic findings : first, sparsely distributed brittle and slow-growing scalp hair, second, a bulbous pear-shaped nose with an elongated philtrum, third brachydactyla with angular deformities. The characteristic radiological features of the hands include cone shaped epiphysis of the proximal interphalangeal joints. Most TRPS type II is sporadic and shows multiple exosteosis, mental retardation and microcephaly in addition to the three chracteristic findings of TRPS type I . A 20-year-old female visited our department for the evaluation of her sparse, slow-growing scalp hair. She also had a bulbous pearshaped nose, ulnar deviated fingers and shortened both hallux. Some relatives of patients show similar symptoms of scalp hair. A Radiologic aly, histopathologic aly and chromosomal study were done, and we established the diagnosis, TRPS type I. (Kor J Dermatol 1997;35(2): 344-349)

UVB 반복조사가 배양 인체 멜라닌세포의 형태학적 변화와 증식 및 멜라닌화에 미치는 영향

김진환,박재경,허충림,이무형 ( Jin Hwan Kim,Jai Kyung Park,Choong Rim Haw,Mu Hyoung Lee ) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.6

피부는 외부에 노출되어 있으므로 외부의 여러 물리적 또는 화학적 요소들에 의해 영향을 받아 변화하는데, 그중 일광은 항상 접하게 되는 필수적 요소의 하나이다. 일광이 피부에 미치는 영향에는 비타민 D의 합성 등의 인체에 이로운 작용외에도 급성반응으로 홍반이나 색소침착, 만성반응으로 나타나는 피부노화, 피부암의 유발 등은 인체에 유해하거나 미용적인 문제를 야기하게 한다. 이러한 피부변화는 표피세포 뿐만 아니라 진피세포들의 복합적인 작용으로 일어나게 된다. 그러나 표피나 진피를 구성하는 세포 각각의 독립적인 변화를 알아보기 위해서는 구성세포의 분리배양이 필요하다. 특히 멜라닌세포는 일광노출에 의해 멜라닌 합성이 증가됨으로써 피부의 색소침착에 중요한 역할을 담당할 뿐 아니라, 이 합성된 멜라닌이 각질형성세포로 이동함으로써 일광 등에 의한 피부의 손상을 방지하게 된다. 멜라닌세포의 성장과 멜라닌화에 영향을 미치는 요소에는 세포의 유전성 프로그램, 일광, 호르몬, 그밖의 멜라닌 대사에 영향을 주는 화학물질 등이 있다. 그중 일광노출에 의한 변화는 주로 자외선B(이하 UVB라고 약함)에 의해 일어나는데, 일반적으로 생체내 실험에서는 멜라닌 세포수가 증가되며 멜라닌화를 촉진하는 것으로 알려져 있다. 그러나 배양 멜라닌세포를 이용한 실험관내 실험에서는 보고자에 따라 다른결과를 보이는데, 이는 자외선의 조사방법, 조사량, 멜라닌세포의 배양방법, 종족이나 개체간의 멜라닌 세포의 특성의 차이 등으로 인해 나타난 것으로 생각된다. 특히 조사량과 함께 조사횟수 즉, 일회조사한 경우와 반복조사한 경우의 멜라닌세포수나 멜라닌 양은 다르게 나타난다. 저자는 일상생활에서의 일광노출과 유사한 적은 양의 UVB를 배양 인체 멜라닌세포에 반복 조사하여 멜라닌세포의 성장과 분화의 변화를 알아보았다. 또한 동일량의 UVB를 분할하여 반복조사한 경우와 일회조사한 경우에 어떤 차이가 있는지 알아봄으로써 UVB가 실험관내 실험에서 멜라닌세포의 성장과 분화에 미치는 영향을 규명하고자 본 실험을 시행하였다. Background:In the skin, the major stimulus for cutaneous pigineatation is ultraviolet radiation. The most important physiologic role of melanin is protection against harmful UV radiation to skin. It is known there are some differences in melanization between a single and multiple exposures of UB, in vivo. Little if known about the functions of the melanocyte alone in cutaneous pigmentation after ultraviolet exposure, because of the complexity of interactions in the whole epidermis Objective:To investigate the effects of multiple exposures at various dosages of UVB, and to compare the effect of UVB in multiple divided exposures ? a single exposure at the same total dosage of UVB on proliferation and melanization in cultured human melanocyte. Methods:Melanocytes were cultured by modified TIC medium The melanocter were exposed daily for three consecutive days to UVB at 2, 4, 8, and 16 mJ/㎠ and a single exposure at 24 mJ/㎠. The morphologic changes were examined by phase contrast microscopy. The melanocytes were counted by hemocytometer and melanin content;were assayed by spectro-photometer. Results:1. The effects of multiple UVB exposures: 1)The morphologic changes were as follows:With three time exposures at a dosage of 8 mJ/㎠, the melanocytes enlarged in size, and elongated their dendrites slightly;with three time exposures at a dosage of 16 mJ/㎠, enlargement in sized and elongation of dendrited were more significant. 2) With three time exposures at dosages of 2 and 4 mJ/㎠, the proliferation of melanocytes was stiumlated significantly(p<0.05). However, with three time exposures at dosages of i and 16 mJ/㎠ the proliferation was inhibited(p<0.05). 3) With three time exposures at dosages of 2 and 4 mJ/㎠, the melanin contents were decreased. However, with three time exposures at a dosage of 16 mJ/㎠, the melanin contents were highly increased(p<0.01). 2. The comparison between multiple divided exposures and a single exposure at the same toal dosage of UVB: 1) There were no morphologic differences of dendrities between with three time exposures at a dosage of 8 mJ/㎠ and with a single exposure at a dosage of 24 mJ/㎠. However enlarged melanocytes were more numerous with a single exposure. 2) The proliferation of melanocytes was more inhibited with:single exposure than with multiple divided exposures(p<0.05). 3) The melanin contents were more increased with a single exposure than with multiple divided exposures(p<0.05). Conclusion:With multiple exposures at lower dosages of UVB, the proliferation of melanocytes was stimulated, and melanixation was decreased. However, with multiple exposures at higher dosages of UVB, the proliferation was inhibited, and melanization was increased. At the same total dosage of UVB, the proliferation on was more inhibited, and the melanization was more increased with a single exposure than with multiple divided exposures.(Kor J Dermatol 1994;32(6):1035∼1045)

Bleomycin 정맥 주사 후 발생한 편모충양 색소침착 3예

조항래,이무형,허충림 ( Hang Rae Cho,Mu Hyoung Lee,Choong Rim Haw ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.2

Bleomycin is an antitumoral antibiotic derived from Streptomyces verticillus in 1965. The drug has been used with a varying success in the treatment of squamous cell carcinomas and malignant lymphomas. The cutaneous toxicities of bleomycin include hyperpigmentation, sclerosis, gangrene, nail changes and erythema. Linear streaks appear to be a characteristic eruption induced by bleomycin. We report three cases of flagellate pigmentation from intravenous bleomycin. Three patients were suffering from uterine cervical carcinomas and were treated with chemotherapy with a VBP (vincristine, bleomycin, cisplatire) regimen. During or after their courses of chemotherapy, dark brownish skin lesions developed on the body surface of these patients. A Light microscopic study of each specimen showed increased pigmentation of the basal layer and an electron microscopic study showed a slightly increased number of melanosomes in keratinocytes. (Kor J Dermatol 1997;35(2): 317-321)

각질형성세포에서 HLA - DR 항원의 발현이 IL - 1 생산에 미치는 영향에 관한 연구

심우영,박재경,허충림 ( Woo Young Sim,Jai Kyung Park,Choong Rim Haw ) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.6

N/A There is increasing evidence that keratinocytes may parcipitate in the inflammatory processes and immune response of skin. Keratinocytes can be induced to express functional immunocompetent molecules such as HLA-DR antigen. In additior, they secrete a wide range of infiammatory cytokines including IL-1. In order to better understand the immunologic role of keratinocyte, it is necessary to quantitate the amount of IL-1α produced by cultured human keratinocytes and evalute the effect of HLA-DR expression on the production of IL-1α by keratinocytes. In this study, we employed the radioimmunoassay to measure the amount of IL-1α produced by cultured human keratinocyte. Human keratinocytes were isolated from neonatal foreskins and grown in MCDB 153 medium. HLA-DR expression was induced by 30 U/㎖ of r-IFN-?. The supernatants were assayed for IL-1α. The results were as follows : 1. When culture medium was not replaced until 72 hours, cell numbers of kerantinocytes without treatment of r-IFN-? were 0.80±0.26×10^6, 1.16±0.21×10^6, 1.33±0.21×10^6 at 24, 48 and 72 hours, respectively. When culture medium was replaced every 48 hours, cell numbers of keratinocytes with treatment of r-IFN-? were 0.72±0.29×10^6, 1.06±0.26×10^6 and 1.06±0.25×10^6 at 24, 48 and 72 hours, respectively. Antiproliferative effect of r-IFN-? was significant at 48 and 72 hours(p=0.046, p=0.003). When culture medium was replaced every 48 hours, cell numbers of keratinocytes without treatment of r-IFN-? were 1.16±0.21×10^6, 1.69±0.27×10^6, 2.01±0.18×10^6 at 48, 96 and 144 hours, respectively. Cell numbers of keratinocytes with treatment of r-IFN-? were 1.06±0.26×10^6, 1.51±0.20×10^6 and 1.67±0.17×10^6 at 48, 96 and 144 hours, respectively. A?tiproliferative effect of r-IFN-? was significant at 48 and 144 hours(p=0.046, p=0.0001). 2. Relatively lower concentration of IFN-?(30 U/㎖) can induce HLA-DR expression on keratinocytes. The percent of HLA-DR positive keratinocytes were 42.15±4.87% by 48 hours. Then it decreased to 14.26±2.85% by 144 hours. 3. When culture medium was not replaced, the levels of IL-1α from supernatants of cultured keratinocytes were 1.513±0.774, 0.778±0.299 and 0.720±0.274 fmol/10^7cells at 24, 48 and 72 hours, respectively. The level is highest at 24 hours and then i? decreased. 4. When the percentage of HLA-DR positive keratinocytes were relatively high 42.15±4.87% and 38.59±3.74% at 48 and 72 hours, respectively, the levels of IL-1α were 1.009±0.320 and 1.002±0.301 fmol/10^7cells, respectively. They were significantly higher than r-IFN-? untreated groups, of which IL-1α levels were 0.778±0.299 and 0.720±0.274 fmol/10^7cells, respectively(p=0.059, p=0.007). When the percentage of HLA-DR positive keratinocytes were decreased, the differences of IL-1αlevel between two groups were not significant. In summary, the results indicate that the amount of IL-1α produced by keratinocytes are relatively low and keratinocytes may participate in the immune process of the skin, and that the immunologic role of HLA-DR^+ keratinocytes might be media?ed via an increased production of IL-1α from HLA-DR^+ keratinocytes.

원저 : Henoch - Schonlein 자반증의 직접면역 형광검사 소견에 관한 연구 ( Original Article : Direct Immunofluorescence Findings in Patients with Henoch - Schonlein Purpura )

김낙인(Nack In Kim),허충림(Choong Rim Haw),홍대광(Dae Kwang Hong) 경희대학교 경희의료원 1986 慶熙醫學 Vol.2 No.1

증례 : 3 남매에서 발생한 우췌양 표피이형성증 3 예 ( Case Report : 3 Cases of Epidermodysplasia Verruciformis occurred in sibling )

김낙인(Nack In Kim),허충림(Choong Rim Haw),홍대광(Dae Kwang Hong),하태영(Tae Young Har),이무형(Moo Hyung Lee) 경희대학교 경희의료원 1986 慶熙醫學 Vol.2 No.3

배양된 인체 멜라닌 세포의 증식과 HLA - DR 항원 표현에 대한 재조합 감마 인터페론의 영향

어수동,이무형,엄주용,박재경,허충림 ( Soo Dong Ahu,Mu Hyoung Lee,Joo Yong Eom,Jai Kyung Park,Choong Rim Haw ) 대한피부과학회 1993 大韓皮膚科學會誌 Vol.31 No.3