The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Yuxia Li,Hua Bai,Zhongzu Zhang,Weihua li,Lei Dong,Xueju Wei,Yanni Ma,Junwu Zhang,Jia Yu,Guotao Sun,Fang Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-co-ding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2

Li, Yuxia,Bai, Hua,Zhang, Zhongzu,li, Weihua,Dong, Lei,Wei, Xueju,Ma, Yanni,Zhang, Junwu,Yu, Jia,Sun, Guotao,Wang, Fang Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.3

MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

Optimization of Physical Education Teaching Mode in Colleges based on Data Mining Clustering Algorithm

Runzhong Li,Yuxia Wang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.10

Deep neural networks (DNNs) and their learning algorithms have been widely used in large data analysis. With the practice of teaching network reform in higher education, many colleges began to use the network to change the traditional teaching methods, and made several achievements. In this paper, the authors first analyze the infinite depth neural network, and construct evaluation index system by using network data mining method. The result shows that 58.09% students think the evaluation from college physical education is formative assessment, 27.94% students think that the evaluation reflects the magnitude progress, and 30.15% students think that it reflects the students' self-evaluation. Physical teaching evaluation is an important part of physical education; however, the survey found that physical education students are lack of understanding of teaching evaluation. Therefore, colleges should make comprehensive evaluation of physical education, and formulate scientific and rational teaching evaluation concept.

Adsorption of different molecular weight lignosulfonates on dimethomorph powder in an aqueous system

Zhili Li,Yuxia Pang,Yuanyuan Ge,Xueqing Qiu 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1

Different molecular weight lignosulfonates were acquired by ultrafiltration and their adsorption behavior on dimethomorph powder were investigated. The results showed that as the molecular weight of lignosulfonates increased the adsorption amounts increased and the adsorption processes were driven by van der Waals forces and hydrophobic effects. The adsorption isotherms were fitted well by Langmuir model. Relations of adsorption amounts and zeta potentials with particle size of dimethomorph particles were established to disclose the dispersing mechanism of dimethomorph suspensions. The average particle sizes were determined by zeta potentials rather than adsorption amounts, which confirmed the dispersing mechanisms were electrostatic repulsive forces.

Identification of genes involved in inbreeding depression of reproduction in Langshan chickens

Xue Qian,Li Guohui,Cao Yuxia,Yin Jianmei,Zhu Yunfen,Zhang Huiyong,Zhou Chenghao,Shen Haiyu,Dou Xinhong,Su Yijun,Wang Kehua,Zou Jianmin,Han Wei 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources. Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens.Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq).Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction.Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

Molecular cloning, subcellular localization, and expression of BsWRKY51 gene from Bletilla striata

Wang Shuangshuang,Zheng Yuxia,Dou Quanli,Zhang Zhengling,Zeng Boping,Li Ying,Qian Yongchun,Ma Li 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2

The WRKY transcription factor family plays a key role in plant growth and development, hormone signaling, and resistance to environmental stress. In this study, we investigated the gene sequence, subcellular localization, and response pattern of a member of the WRKY transcription factor family to reveal its protein structure and involvement in the resistance signaling pathway.The BsWRKY51 gene was cloned by RT-PCR, and the structural characteristics of its encoded protein WRKY51 were analyzed by bioinformatics. The vector was next transiently transformed into tobacco to analyze the subcellular locali- zation, and real-time fluorescence quantitative PCR was performed to analyze the changes in the expression pattern of BsWRKY51. The BsWRKY51 gene has a coding sequence (CDS) length of 987 bp.The respective unstable hydrophilic protein BsWRKY51 is localized in the nucleus. It most closely related to the WRKY protein of Dendrobium catenatum in the Orchidaceae family. Fluorescence quantitative PCR results showed that the BsWRKY51 expression in the leaves was significantly higher than that in the roots, stems, and pseudobulbs of Bletilla striata seedlings. Under the conditions of salt and drought stress, the BsWRKY51 expression gradual increased and then a slightly decreased, and under salicylic acid (SA) treatment, the expression of BsWRKY51 showed an overall decreasing trend.The BsWRKY51 gene of Bletilla striata may play an important regulatory role in its salt and drought stress responses. Our present findings provide the foundation for elucidating the mechanisms of salt and drought tolerance in Bletilla striata and for breeding new varieties.

Green and mild production of 5-aminolevulinic acid from algal biomass

Binglin Chen,Jiachen Li,Yunchao Feng,Kai Le,Yuxia Zai,Xing Tang,Yong Sun,Xianhai Zeng,Lu Lin 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5

Algal biomass was converted into 5-aminolevulinic acid (5-ALA) in five chemical steps: conversion to 5- (chloromethyl)furfural (5-CMF), ammoniation, ring-opening (photo-oxidation), reduction, and hydrolyzation. Among them, we mainly focused on the 5-CMF production and the following ammoniation. To our knowledge, the mixed solvent catalytic system of deep eutectic solvent (DES) and low concentration hydrochloric acid is the first reported for the synthesis of 5-CMF from algal biomass, providing a 24.6% 5-CMF yield at 120 oC for 5 h. Potassium phthalimide (KPI) was employed as an ammoniation reagent with superb selectivity and activity instead of conventional sodium azide (NaN3). Optimizing the experimental design, a 23.7% 5-ALA yield along with high purity (>96%) was achieved from 5-CMF, and the total 5-ALA yield was 5.8% from algal biomass. This work provides a green and mild pathway for 5- ALA production from algal biomass.

Carbon Source Affects Synthesis, Structures, and Activities of Mycelial Polysaccharides from Medicinal Fungus Inonotus obliquus

( Huihui He ),( Yingying Li ),( Mingyue Fang ),( Tiantian Li ),( Yunxiang Liang ),( Yuxia Mei ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.6

The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated. After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups. Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source. Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa. Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios. The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions. In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and antitumor activities of NIOPs were substantially affected by carbon source. qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source. Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.

Insect cytokine growth-blocking peptide may regulate density-dependent phase trait of cuticular melanization in the larval armyworm, Mythimna separata

Zhang Yingchao,He Juan,Zhang Yuxia,Li Yue,Yu Yang,Jiang Xingfu,Pan Weidong 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Growth-blocking peptide (GBP) is a 25 amino acid insect cytokine found in lepidopteran insects that has diverse biological activities such as larval growth regulation, paralysis induction, cell proliferation and stimulation of immune cells. Density-dependent phase polyphenism is a phenomenon of phenotypic plasticity in which the expression of a variety of traits can be affected by local population density. In the present study, the armyworm Mythimna separata larvae with four rearing densities (1 larva/vial, 2 larvae/vial, 4 larvae/vial and 6 larvae/vial) were tested for cuticular melanization and body weight throughout the third-fifth instar, and the functional role of GBP in regulating the changes was investigated. The results indicated that when reared at high densities, the larvae exhibited less body weight and more degree of cuticular melanization than larvae reared at low densities. The gene expression of GBP in armyworm larvae showed an initial rise and then decline trend with increased rearing densities in the third to fifth instar. Compared with control, more degree of cuticular melanization was observed in GBP-injected larvae (500 ng/larva in volume 50 μL) than that in Ringer’s solution-injected counterparts. Furthermore, the gene expression level of dopa decarboxylase and prophenoloxidase increased significantly in GBP-injected fifth instar larvae from 6 h to 12 h after injection, suggesting the role of GBP in modulating density-dependent phase trait of armyworm cuticular melanization.

Nonlinear Adaptive Backstepping with ESO for the Quadrotor Trajectory Tracking Control in the Multiple Disturbances

Jie Liu,Wendong Gai,Jing Zhang,Yuxia Li 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.11

In this paper, we present a nonlinear adaptive backstepping with extended state observer (ESO) trajectory tracking controller for a quadrotor unmanned aerial vehicle (UAV) subject to the multiple disturbances, which include the parametric uncertainties, actuator faults and external disturbance. First, a six-degrees of freedom quadrotor UAV model with the multiple disturbances function is built. Second, the adaptive backstepping controller is designed to track the desired trajectory command aim at internal disturbance. And the adaptive backstepping controller with ESO is designed to track the desired trajectory command aim at external disturbance. Third, the stability of the system is proved by the circle criterion. Finally, under different flight scenarios, simulation results are given to demonstrate the effectiveness of the proposed method.