Analysis of Rate Controlling Factors in the Kraft Pulping Process

Yoon, Sung Hoon,Peter Labosky Jr . 한국공업화학회 1996 Journal of Industrial and Engineering Chemistry Vol.2 No.1

Aspen (Populus tremuloides) and Nonway spruce (Picea exelsa) wood chips were subjected to 170℃ isothermal laboratory kraft pulping. Hypothetical spherical wood core models were used in the analysis of the rate limiting factor in the kraft pulping process. Under the assumption of a heterogeneous reaction, the carbohydrate dissolution reaction in the kraft pulping process appeared to be limited by the molecular diffusion process. In the kraft delignification process, however, no rate-controlling factor was determined using the heterogeneous model analysis. A volumetric reaction model formulated under the assumption of semi-heterogeneous reaction showed the rate limiting factor in the kraft delignification to be a chemical reaction in both wood species.

정상 및 임신성 고혈압 임신의 태반 내 Gelatinases의 발현 양상에 관한 연구

노정래,문종수,양순하,황종대,오원종,최정주,윤병구,이제호 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.6

목적 : 정상과 임신성 고혈압 임신에서 태반 내 gelatinases의 활성도와 조절 인자들의 발현을 비교해보고자함. 연구 방법 : 각8개의 정상 및 임신성 고혈압 태반을 대상으로 gelatinase A와 B의 활성도는 gelatin zymography를 이용하여 비교하였고 MMP-2, TIMP-2와 MT1-MMP의 발현정도는 Northern blot을 이용하여 비교하였다. 결과 : MT1-MMP 및 TIMP-1,2의 mRNA의 발현정도는 정상 및 임신성 고혈압 임신 사이에 차이가 없었으나 MMP-2와 MMP-9 mRNA의 발현은 정상 임신의 경우에 임신성 고혈압 임신에 비하여 높게 나타났다. 한편 zymogram을 이용한 MMPs의 활성도를 살펴보면 proMMP-2와 proMMP-9의 활성도가 정상 임신의 경우에 임신성 고혈압 임신에 비하여 높았으나 62kDa의 active form의 활성도는 두 군 간에 차이가 없었다. 그러나 분만 진통의 영향을 배제시킨 후에는 Northern blot에서 MMP-2, MMP-9, TIMP-1, TIMP-2 및 MT1-MMP mRNA의 발현 정도에는 정상 및 임신성 고혈압 임신 간에 유의한 차이가 없었고, zymogram의 proMMP-2 및 proMMP-9의 활성도에도 정상 및 임신성 고혈압 임신간에 유의한 차이가 없었다. 결론 : 임신성 고혈압 임신에서 proMMP-2와 proMMP-9의 활성도가 정상 임신에 비하여 높았지만 이는 분만 진통의 영향으로 생각되고, 태반 내 MT1-MMP가 MMP-2와 MMP-9의 활성화 과정에 조절 작용을 하는 것으로 여겨지나 향후 폭 넓은 연구가 필요하다. Objectives : The aim of this study was to compare activities of gelatinases and their regulators in normal and preeclamptic placenta tissue. Methods : The activities of gelatinase A and B were evaluated by gelatin zymography in eight preeclamptic and 8 normal placentas. The levels of MMP-2, MMP-9, TIMP-1, TIMP-2 and MTI-MMP were measured by Northern blotting and gelatin zymography. Results : Densitometric measurements of gelatin zymogram revealed decreased activities of 92 kDa proMMP-9 and 72kDa proMMP-2 activity in preeclamptic placentas compared to normal placentas. The activities of 62 kDa active MMP-2, however, were not different between normal and preeclamptic placentas. The level of MMP-2 and MMP-9 mRNA was lower in preeclamptic placenta than in normal placenta. On the other hand, there was no difference in MT1-MMP and TIMP-2 mRNA level between normal and preeclamptic placenta. To the contrary of our expectation, increased level of poMMP-2 and proMMP-9 activities in placenta was associated with labor. Conclusions : Decreased activities of gelatinases may contribute to defective trophoblastic invasion of maternal tissues in preeclampsia. The MT1-MMP ssems to be a main determinant of gelatinase activities in human placenta. The mechanism that regulates activities of other MMPs in human placenta needs further investigations.

원인대의 원발성 평활근종 1 예

김정란,윤혜원,임문환 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.11

동국대학교 의과대학 산부인과에 내원한 37세의 경산부에서 원인대의 평활근종 1예를 치유한 바 있어 이에 문헌고찰과 함께 보고하는 바이다. A 37-year old patient was surgically treated for a tumor the size of a mans fist, located on the right of the uterus and appeared to be either a ovarian tumor or an uterine myoma. Laparotomy revealed a solid tumor in the uterine round ligament, about 3 cm apart from the uterine horn, and histologically diagnosed as a leiomyoma. We have experienced a case a leiomyoma in the round ligament and it is reported witha brief review.

자궁경부의 상피내암과 침윤성암에서 세포증식능 표지자로서의 Heat Shock Protein-70과 Ki-67의 발현에 관한 연구

김정란,심재철,윤혜원,배철성,양회생,최석철,도은형,이종임 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.5

본원에서 자궁경부 상피내종양과 침윤성 편평상피암종으로 확진된 환자의 자궁경부조직 50예(CIN Ⅰ/Ⅱ 14예, CIN Ⅲ 18예, 침윤암 18예)를 대상으로 HSP 70 및 Ki-67에 대한 면역조직 화학적 염색을 시행하여 각각의 발현 유무와 상호 연관성을 조사하여 다음과 같은 결과를 얻었다. 1. 침윤성 편평상피암종에서 HSP 70의 높은 발현 율 및 강도를 보였으며(p<0.05) 분화가 좋은 암종에 서 더 높은 양성률 및 강도를 보였으나 통계학적 유 의성은 없었다(p=0.124). 2. HSP 70의 발현율 및 HSP 70의 염색지수는 암 종이 전암단계에서 침윤암으로 진행함에 따라 증가 하는 경향을 보였고 상피내종양과 침윤암 사이에는 통계적으로 유의한 차이가 있었다(p<0.05). 3. Ki-67의 양성 세포비율은 자궁경부 병변이 심 화될수록 유의하게 증가하였다(p<0.05). Ki-67 항원 표지자에 염색된 양성 세포는 정상상피와 편평화생 세포에서는 양성 세포가 기저층과 부기저층에만 국 한되었으며 CIN Ⅰ/Ⅱ는 중간층까지, CIN Ⅲ과 침윤 암에서는 상피전층에 걸쳐 분포하였다. 4. 자궁경부의 상피내종양과 침윤암에서 HSP 70 의 염색지수가 증가할수록 Ki-67의 양성 세포 비율이 높게 나타났으나, HSP 70의 염색지수와 Ki-67 양성 세포 비율의 상관관계에 있어서는 통계적으로 유의한 차이가 없었다. 이상의 결과로 HSP 70은 상피내 병변과 암종 사 이에 뚜렷한 발현차를 보여 상피내 병변이 침윤암종 으로 진행하는데 일정한 역할을 할 것으로 사료되 며, 향후 HSP 70의 정량적인 분석이 가능하게 될 경 우 HSP 발현이 상피내종양의 악성화 예측에 유용한 표지자로 이용할 수 있을 것으로 추정되었다. Ki-67 의 발현이 양성 세포비율 또한 자궁경부 병변이 진행할수록 유의하게 증가하는 것으로 보아 HSP 70과 Ki-67의 발현 정도가 세포증식능 표지자로서 가능성이 있음을 보여주었다. Heat shock protein (HSP) expressions is upregulated in some tumor cells. Furthermore, the 70-kda HSP (HSP70) is implicated in the degree of tumor differentiation, the rate of tumor proliferation and the magnitude of the antitumor immune reponse. Accordingly, the distribution and intensity of HSP 70 expression were assessed in normal squamous epithelium, squamous metaplasia and 32 cervical intraepithelial neoplasm (CIN, 14 CIN Ⅰ, Ⅱ and 18 CIN Ⅲ) and 18 squamous cell carcinoma (SCC) of uterine cervix with Ki-67 cell proliferation associated antigen by avidin biotin complex immunohistochemisrty. Staining intesity was recorded in scoring index. Specific staining with anti-HSP 70 antibody was exclusively confined to the cytoplasm. HSP 70 was expressed in normal squamous epithelium (66.7%). Metaplastic squamous cells were completely negative. Rate of positive cases of HSP 70 expression were 35.9% in CIN Ⅰ/Ⅱ, 50% in CIN Ⅲ, 89% in carcinomas (100% in keratinizing, 85% in nonkeratinizing) and scoring index of HSP 70 were 0.57 in CIN Ⅰ/Ⅱ, 0.61 in CIN Ⅲ, 1.50 in carcinomas (2.00 in keratinizing, 1.31 in nonkeratinizing). The distribution and intensity of HSP 70 expression in keratinizing SCC was greater than that in nonkeratinizing SCC, although this result just failed to reach stastistical significance. Results of staining HSP 70 were correlated with distribution and rate of Ki-67 antigen expression but there was no significant regression coefficience between these groups. The expression and intensity of HSP 70 had no overlap between CIN and SCC, which can be proposed as a useful marker for the detection likely to be at risk of progression of CIN to SCC.

Targeting of Recombinant Adenovirus to Hepatocellular Carcinoma Cells using a Tumor Specific Bifunctional Fab Comples

Wands,JR,Yoon,SK,Mohr,L,Oiordan,C,Armentano,D 가톨릭중앙의료원 가톨릭암센터 1998 암심포지움 Vol.- No.2

Aims: Recombinant adenovirus vectors are very attractive for gene delivery if specifically targeted malignent cells. In this study we developed an approach to target adenoviral vectors using AF-20 mAb that binds to a 180 kDa antigen which is highly expressed on HCC cells using a bifunctional Fab complex (Bi-Fab) to induce tumor cell specific gene delivery.

임신성 고혈압질환과 안지오텐신전환효소 유전자의 유전적 다형성과의 연관성에 관한 연구

이제호,윤병구,배덕수,정재현,노정래,양순하,김덕경 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.6

Background: The angiotensin coverting enzyme(ACE) gene(encoding kininase II, EC 3.4.15.1) contains a polymorphism based on the presence(insertion [I]) or absence(deletion [D]) within an intron of a 287bp nonsense DNA domain, resulting in three genotypes(D/ I) and I/I homozygotes, and I/D heterozygotes). Alu insertion is associated with lower ACE level than deletion allele(D) and it was observed that D/D individuals have twice the ACE activity of I/I patients. Pregnancy induced hypertension(PIH) probably results from dominating pressor systems owing to loss of antagonizing vasodilator autacoids. Angiotensin II is an extremely potent arteriolar vasoconstrictor. Overactivity or failure to supress responsiveness to the increased activity of angiotensin II, which is generated by ACE, would seem to be a reasonable basis for the vasoconstriction of PIH. The aim of this study is to evaluate the relationship between ACE genotype and PIH. Methods: Blood sampling was taken from 39 patients with PIH. The hypertensive disorders, confirmed at postpartum follow up, were classified as gestational hypertension without proteinuria, preeclampsia(mild and severe) and eclampsia. The diagnosis of preeclampsia was made according to the American College of Obstetrics and Gynecology criteria of hypertension and proteinuria($gt;300 mg/24 hr urine). Genomic DNA was extracted from blood sample. After PCR amplification of the respective fragments from intron 16 of the ACE gene, size fractionation and visualization by electrophoresis were performed. Results: PIH group(including gestational hypertension, mild and severe preeclampsia : frequency of I allele 0.756 and D allele 0.244) had more I allele and less D allele when compared with normal population(frequency of I allele 0.609 and D allele 0.391)(p$lt;0.05). And PIH group had more I/I homozygote individuals showing significant distortion from Hardy-Weinberg equilibrium of ACE genotype(p$lt;0.05). Moreover, severe preeclampsia group alon(frequency of I allele 0.759 and D allele 0.241) had more I allele and less D allele when compared with normal population and had significantly more I/I homozygote individuals. Conclusion: As pregnancies with PIH had more ACE I allele and I/I homozygote individuals. PIH could be associated with I allele of the ACE gene. Considering the observed codominant association between the D-I polymorphism and plasma ACE activity, our result is in favor of the thesis that PIH primarily arises from defective synthsis of vasodilating autacoids and renin-angiotensin system exerts secondary vasoconstrictive action. However, the relationship between ACE genotype and defective vasodilating mechanism during pregnancy is unknown at present.

Complication of Percutaneous Intra-articuclar Transtendon Biceps Tenodesis - Two Cases Report

Lee WS,Yoon JR,Kim YB,Kim JK,Kim KH 대한견주관절의학회 2014 대한견주관절학회 학술대회논문집 Vol.2014 No.3

Neurotrophic arthritis of the elbow due to syringomyelia

Lee WS,Yoon JR,Kim YB,Oh SR,Lee JW,Kim JK 대한견주관절의학회 2013 대한견주관절학회 학술대회논문집 Vol.2013 No.3

Tophaceous gout of the rotator cuff

Lee WS,Yoon JR,Kim YB,Oh SR,Lee JW,Kim JK 대한견주관절의학회 2013 대한견주관절학회 학술대회논문집 Vol.2013 No.3

Detection and Characterization of a Lytic Pediococcus Bacteriophage from the Fermenting Cucumber Brine

( Sung Sik Yoon ),( Roudolphe Barrangou Poueys ),( Fred Breidt Jr ),( Henry P. Fleming ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.2