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한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석
장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1
Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.
한국인에서 DXS7132 유전좌와 GATA31D10 유전좌의 다형성에 관한 연구
안종성,장영길,이숭덕,신창호,이윤성,이정빈 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1
The validation study for two STR loci on X-chromosome, DXS7132 and GATA31D10, was done including allelic distribution and frequency of each allele to use these results for individual identification and paternity testing. For 496 unrelated Koreans, above two STR loci were amplified simultaneously using duplex PCR amplification method. The amplified products were analyzed by polyarylamide gel electrophoresis followed by silver staining. In male DXS7132 locus revealed 7 different alleles ranging from 276bp to 300bp. The largest allele was consisted of 14 repetition of [TCTA] unit and took 0.3417. The allele 15 followed next as 0.3165 and allele 13 as 0.1726. In female general distribution was same except one allele, allele 18 was found additionally. The heterozygosity was 0.7706 and 23 different genotypes were found. Polymorphism information content(PIC) was 0.727. Two cases of mutation were noted in DXS7132 locus In both male and femal 7 different alleles were noted in GATA31D10 locus and the alleles ranged from 195bp to 231bp. The allele 15(199bp) took the majority of all as 0.825. The other alleles showed rather relatively low frequency. The heterozygosity was 0.2385 and 11 different genotypes were found. PIC was 0.2521, and no mutation was noted in GATA31D10 locus. Considering these two loci together, 22 different halpotype were noted.
Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3
Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.
Constructions of Generalized Bent Boolean Functions on Odd Number of Variables
Yong-Bin Zhao,Feng-Rong Zhang,Yu-Pu Hu 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.5
In this paper, we investigate the constructions of generalized bent Boolean functions defined on with values in Z4. We first present a construction of generalized bent Boolean functions defined on with values in Z4. The main technique is to utilize bent functions to derive generalized bent functions on odd number of variables. In addition, by using Boolean permutations, we provide a specific method to construct generalized bent functions on odd number of variables.
High Precision FE Modeling for Predicting Inner Polygon Defect of Hot Rolled Seamless Steel Tubes
Yong-Zheng Jiang,Yong-Zheng Jiang,Xiao-Ping Zhang,Kuan-Fang He,Guan-Fu Bin 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.11
A high precision FE model is the key basis for cause analysis and overcoming of inner polygon defect of hot rolled seamless steel tubes. In this paper, the viscoelastic-plastic FEM is adopted in order to develop a high precision FE model for predicting the defect of inner polygon. Mechanical properties of tube material under rolling environment is obtained through five sets of high temperature compression tests, thus the viscoelastic-plastic constitutive equation of tube material is regressed and agrees with the tests results. Heat transfer boundary conditions, roll constant speed and contact friction boundary conditions are applied simultaneously on the FE model and thermo-mechanical coupled explicit algorithm is adopted for solution. The precision of the FE model is verified through industry experiments. Results shows the simulated inner wall shape is in good accordance with the experiment results, and the friction force, stress, strain and temperature distribution in the deformation zone are also discussed. It can be concluded that the viscoelastic-plastic FE model is of high precision and can be applied for better analysis of the hot rolling results.
Yong Chun Li,Fan Rong Meng,Chun Yan Zhang,Ning Zhang,Ming Shan Sun,Jiang Ping Ren,Hong Bin Niu,Xiang Wang,Jun Yin 한국식물학회 2012 Journal of Plant Biology Vol.55 No.5
To understand better the mechanisms that regulate the water stress response in wheat, we conducted a comparative analysis of transcript profiles in roots from two wheat genotypes -- drought-tolerant ‘Luohan No. 2’ (LH)and drought-susceptible ‘Chinese Spring’ (CS). In LH roots,3831 transcripts displayed changes in expression of at least two-fold over the well-watered control when drought treatment was applied. Of these, 1593 were induced while 2238 were repressed. Relatively fewer transcripts were drought-responsive in CS; i.e., 1404 transcripts were induced and 1493 were repressed. In common between LH and CS,569 transcripts were induced and 424 transcripts were repressed. In all, 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories. Among those annotated transcripts from LH and CS that had fold-change ratios of at least 4, 92 induced transcripts were common to both, while 23 transcripts were specifically induced in LH. Gene ontology analysis of these induced genes showed highly significant enrichment for multiple terms related to abiotic stimuli, organic acid biosynthesis,and lipid metabolism. This suggests that these gene groups play important roles during the stress response in LH and CS, and might also be responsible for differences in drought tolerance between those genotypes.
Validation of the fuel rod performance analysis code FRIPAC
Yong-Jun Deng,Jun Wei,Yang Wang,Bin Zhang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.6
The fuel rod performance has great importance for the safety and economy of an operating reactor. Thefuel rod performance analysis code, which considers the thermal-mechanical response and irradiationeffects of fuel rod, is usually developed in order to predict fuel rod performance accurately. The FRIPAC(Fuel Rod Integral Performance Analysis Code) is such a fuel rod performance analysis code that has beendeveloped recently by China Nuclear Power Technology Research Institute Co. Ltd. The code aims at thecomputational simulation of the Pressurized Water Reactor fuel rod behavior for both steady-state andpower ramp condition. A brief overview of FRIPAC is presented including the computational frameworkand the main behavioral models. Validation of the code is also presented and it focuses on the fuel rodbehavior including fuel center temperature, fission gas release, rod internal pressure/internal void volume,cladding outer diameter and cladding corrosion thickness. The validation is based on experimentaldata from several international projects. The validation results indicate that FRIPAC is an accurate andreliable fuel rod performance analysis code because of the satisfactory comparison results between theexperimental measurements and the code predictions.
MULTIPLE PERIODIC SOLUTIONS OF p-LAPLACIAN EQUATION WITH ONE-SIDE NAGUMO CONDITION
Zhang, Jian Jun,Liu, Wen Bin,Ni, Jin Bo,Chen, Tai Yong Korean Mathematical Society 2008 대한수학회지 Vol.45 No.6
In this paper, the existence and multiplicity of solution of periodic solutions of p-Laplacian boundary value problem are studied by using degree theory and upper and lower solutions method. Some known results are improved.
Parallel Control of Shunt Active Power Filters in Capacity Proportion Frequency Allocation Mode
Zhang, Shuquan,Dai, Ke,Xie, Bin,Kang, Yong The Korean Institute of Power Electronics 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.4
A parallel control strategy in capacity proportion frequency allocation mode for shunt active power filters (APFs) is proposed to overcome some of the difficulties in high power applications. To improve the compensation accuracy and overall system stability, an improved selective harmonic current control based on multiple synchronous rotating reference coordinates is presented in a single APF unit, which approximately implements zero steady-state error compensation. The combined decoupling strategy is proposed and theoretically analyzed to simplify selective harmonic current control. Improved selective harmonic current control forms the basis for multi-APF parallel operation. Therefore, a parallel control strategy is proposed to realize a proper optimization so that the APFs with a larger capacity compensate more harmonic current and the ones with a smaller capacity compensate less harmonic current, which is very practical for accurate harmonic current compensation and stable grid operation in high power applications. This is verified by experimental results. The total harmonic distortion (THD) is reduced from 29% to 2.7% for a typical uncontrolled rectifier load with a resistor and an inductor in a laboratory platform.