Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.

유자종실의 Sterol 조성

최상도,주옥수,조용운 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

남해산 柚子種實油을 分析試料로 하여 日本, 基準油脂分析試驗法에 따라서 不檢化物量을 定量하고 T. L. C法으로 不檢化物을 分劃하였으며 G. C法에 依해 sterol組成을 分析하였다. 유자종실기름의 불검화물량은 기름 1g당 19.2mg으로서 1.9%이였고 불검화물의 분획 pattern은 less polar compounds가 20.3%, 4,4-dimethylsterol이 9.6%, 4-desmethylsterol이 47.1% 및 미전개물질이 22.9%이였다. sterol조성은 cholesterol이 0.3%, campesterol이 11.9%, stigmasterol이 9.3%, β-sitosterol이 72.2% 및 未知 sterol이 6.3% 이였다. 불검화물량, 불검화물의 분획 pattern 및 sterol조성은 他植物種實油와 거의 같은 경향을 나타냈다. This experiment was performed to the unsaponifiable matters content of the citrus seed oil. Also, the fractionated sterol pattern and the sterol composition of the citrus seed oil were examined. The citrus seed oil were contained 19.2mg unsaponifiable matters per gram. The unsaponifiable matters in seed oil were fractionated into less polar compounds(20.3%), 4, 4-dimethylsterol(9.6%), 4-monomethylsterol(0%), 4-desmethylsterol(47.1%) and undeveloped matters(22.9%). The sterol composition in citrus seed oil were cholesterol 0.3%, campesterol 11.9%, stigmasterol 9.3% and beta-sitosterol 72.2%. But brassicasterol and avenasterol were not detected.

복합재료 구조물 수리시 발생하는 잔류변형에 대한 덧대기 현상

최항석,이수용 한국항공대학교 1999 論文集 Vol.37 No.-

복합재료 구조물의 덧대기 수리시에 발생하는 잔류변형에 대한 덧대기의 영향을 파악하기 위해서 유한요소 해석을 수행하였다. 유한요쇼 프로그램은 삼차원 응축 쉘 요소와 일차 전단변형 이론에 근거하여 개발되었다. 준등방성, 직교이방성, angle-ply 적층 쉘에 대해 각각 angle-ply, 준등방성, 직교이방성 덧대기 적층으로 수리하였을 때 최종 잔류변형률을 계산하였다. 최대 잔류변형률은 대부분 적층쉘과 덧대기의 인접 부위에서 발생하였고, 그 부위에서 상당히 급격하게 변화하였다. 잔류변형은 적층쉘과 덧대기의 적층순서에 따라 크게 좌우되며, 또한 cutout 영역이 커짐에 따라 일반적으로 잔류변형이 증가하였다. Finite element analysis is performed to investigate the effect of patches on residual deformation induced by the patch repair of composite structures. The finite element program is developed based upon a 3-D degenerated shell element and the first shear deformation theory. Final residual strains are calculated for quasi-isotropic, orthotropic and angle-ply laminated shells repaired with angle-ply, quasi-isotropic and orthotropic prepreg patches, respectively. Maximum residual strains are occurred and abruptly changed near at interfaces of the laminated shell and the patch. Residual deformation is depended on stacking sequences of the laminated shell and the patch, and is generally increased as the region of a cutout is increased.

미계측 유역의 부유물질 산정을 위한 다중회귀식 개발

최한규,박재용,박수진 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

The purpose of this study is to present quantitatively the influence of variables that had the largest effect on the changes in suspended solids(SS), which would cause turbid water phenomenon, among water quality factors of the non-point pollution source, and then to develop a multiple regression equation of SS and predict the water quality of ungaged watersheds so as to provide basic data to establish efficient management plans for SS which flow in rivers and lakes. To identify the correlation of SS with the amount of rainfall and the state of land use, a simple correlation analysis and a simple regression analysis were conducted respectively. Finally, a multiple regression analysis was conducted to provide that SS were set as dependent variables while the amount of rainfall, paddy fields and dry fields were set as independent variables. As a result, the amount of rainfall had the most significant influence on changes in SS, followed by dry fields and paddy fields. In addition, the multiple regression equation was developed to predict SS in unmeasurable watersheds.

CRP* 의존성 maltose 대사 촉진 유전자 sfs4의 클로닝 및 염기배열 결정

최용락,정희태,조무제,정수열 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3

CRP (cAMP receptor protein)은 cAMP와 결합하여 cAMP-CRP 복합체를 형성하여 전사조절의 조절인자로서 작용한다. crp 유전자에 변이를 도입하여 cAMP의 비존재 상태에서 cAMP-CRP와 비슷한 기능을 가진 crp* 유전자가 도입된 대장균 MK2001 (crp*¹, cya::km)을 숙주로 사용하여 cAMP 혹은 cGMP의 비존재하에서도 mal 유전자의 발현을 촉진시키는 유전자 sfs (sugar fermentation stimulation) 수 종을 클로닝하였다. 본 실험에서는 이미 밝혀진 nlp (Ner like protein) 유전자와 같이, sfs의 새로운 유전자를 탐색하여, 그 중 sfs4의 2126bp 전 염기배열을 결정하고, 잠정적인 sfs4의 promoter 영역에는 CRP 단백질과의 결합 DNA 공통 염기배열(5' AAT TGTGA ACACCA TCACC CGT 3')이 존재함을 확인했다. lacZ 융합 유전자를 작성하여 TP2010R1과 MK2001의 균주에서 cAMP를 첨가할 경우 각각 2.3배, 1.8배의 β-galactosidase 활성이 증가 하는 것으로 보아 sfs4는 cAMP-CRP에 의해 발현 조절을 받는 것으로 나타났다 In Escherichia coli, CRP forms a complex with cAMP and acts as a transcriptional regulator of many genes, including sugar metabolism operons. The E. coli MK2001, which is introduced the altered crp*¹, is functional in the expression of lac, ara and man, in the absence of cAMP. However, the expression of mal gene is fully activated by the addition of cAMP or cGMP. The object of the study is cloning of the sfs (sugar fermentation stimulation) genes, which was involved in regulation of mal gene expression with the altered crp*¹gene, and structural analysis and characterization of the genes at the molecular level. We have cloned 5 different E. coli genes which stimulate the maltose metabolism in a crp*¹, cya::km (MK2001) background. Newly identified genes were designated as sfs. One of the sfs genes (pPC1), located at the 53.2 min map position in the E. coli chromosome, was further analyzed. Expression of the genes, which is involved in maltose metabolism, malQ (amylomaltase), was increased to 5.8-fold in the presence of a plasmid, pAP5, containing the subcloned sfs4 gene. The nucleotide sequence of a common 2,126bp segment of the pPCM1 was determined and two open reading frames (ORF1 and ORF2) were detected. The ORF1 encodes the sfs4 gene and ORF2 encodes a truncated protein. Potential CRP binding site is located in the upstream of the putative promoter in the regulatory region. Expression of the cloned sfs4 gene was positively regulated by the cAMP-CRP complex.

슬관절에 히야루론산 주입후 발생한 활막성 연골종증 : 증례 보고

최의성,김용민,김동수,손현철,박경진,조병기,배승환 충북대학교 의과대학 충북대학교 의학연구소 2009 忠北醫大學術誌 Vol.18 No.2

활막성 연골종증은 비교적 드문 양성 종양으로 관절내 활액막 결체조직의 양성 반응성 이형성에 의해 여러 개의 연골성 결절을 형성하고 이것이 관절내로 유리되어 유리체를 형성하는 질환으로 주로 슬관절에 발생한다. 활막성 연골종증의 정확한 발생 기전은 아직 밝혀지지 않았으나 외상, 감염 등이 원인으로 제시되고 있다. 저자들은 슬관절 내 히야루론산 주입후 발생한 슬관절내 활막성 연골종증을 1 례 경험하였으며, 관절경적 제거술을 통해 만족스런 결과를 얻었다. As a relatively rare benign tumor, synovial chondromatosis forms several cartilaginous nodules by the benign reactive metaplasia of synovial connective tissues within joints, which are loosed into the joints and cause a disease forming loose body mainly in knee joint. Accurate mechanism of synovial chondromatosis has not been clarified yet; however, trauma, infections and others have been suggested as its possible causes. The authors of this study experienced an example of a patient with synovial chondromatosis in knee joint occurred after the injection of hyaluronic acid in the joints and had a satisfactory outcome through the arthroscopic resection.

증류식 소주 제조 중 고급 알콜류의 생성

최진상,이용수 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

백미원료와 대맥원료의 일반성분은 큰 차이가 없었지만, 전분가는 백미원료가 대맥원료보다 3.4% 정도 높았다. 백미원료와 대맥원료의 알콜 수율은 백미원료가 우수하였으며, 대맥원료는 산의 생성량이 많았다. 효모별 발효율은 위스키 효모인 Saccharomyces cerevisiae Suntory균이 가장 높았고, 다음으로 Sake, Mikada 및 Hansen 균의 순이었다. 원료별 고급 알콜류로서 n-propanol, iso-butanol 및 iso-amyl alcohol 등을 본석하였으며, 고급 알콜류의 생성량은 백미원료가 대맥원료보다 높았으나 생성 특성에는 큰 차이가 없었다. 효모종류별 고급 알콜류의 생성특성은 유사하였지만, 그 생성량에서는 약간의 차이가 있었다. General compositions were not difference between rice and barley, bur carbohydrate value was higher in rice than barley as above 3.4%. Alcohol ratio was better in rice than barley, but barley had higher contents in the formation of acids. Fermentation rate of yeast varieties vas best in Saccahromyces cerevisiae Suntory and the next order was Sake, Mikada and Hansen species. Higher alcohols were more in rice than in barley, but the characteristic of formation was not difference. And the formation of yeast varieties had little difference between species but the characteristic of formation was similar to four species.

미계측 유역의 부유물질 산정을 위한 다중회귀식 개발

최한규,박재용,박수진 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

The purpose of this study is to present quantitatively the influence of variables that had the largest effect on the changes in suspended solids(SS), which would cause turbid water phenomenon, among water quality factors of the non-point pollution source, and then to develop a multiple regression equation of SS and predict the water quality of ungaged watersheds so as to provide basic data to establish efficient management plans for SS which flow in rivers and lakes. To identify the correlation of SS with the amount of rainfall and the state of land use, a simple correlation analysis and a simple regression analysis were conducted respectively, Finally, a multiple regression analysis was conducted to provide that SS were set as dependent variables while the amount of rainfall, paddy fields and dry fields were set as independent variables. As a result, the amount of rainfall had the most significant influence on changes in SS, followed by dry fields and paddy fields. In addition, the multiple regression equation was developed to predict SS in unmeasurable watersheds.