Expression Study of a Recombinant Plasmid containing Dipeptidyl Peptidase-4 Gene in E. coli: A Plausible Application for Celiac Disease Patients to Digest Gluten

Lee, Yeonjae,Kang, Ryan,Kwon, Jenna,Jo, Kyuhee,Im, Jungbin,Jung, Sangwook,Lee, DongHyun,Lee, Juhyeon,Lee, Jeong-Sang The Institute of Internet 2018 International journal of advanced smart convergenc Vol.7 No.2

Celiac disease (CD) is an immune-mediated enteropathy of small intestine diagnosed in both childhood and adulthood. CD is caused by gluten, which produces gliadorphin during its digestion. The enzyme dipeptidyl peptidase-4 (DPP4) breaks gliadorphin down nevertheless the last tripeptide remains and eventually inhibits DPP4, thus slowing down its process. Therefore, the idea is to produce an additional DPP4 enzyme which is crucial. Consequently, the functional DPP4 gene was cloned into pCDNA3 intermediate (FLAG+DPP4) vector and finally a recombinant plasmid pSB1C3 (Andersons promoters+FLAG+DPP4) was constructed using synthetic biology. Normally, a DPP4 inhibitor is used as a cure for diabetes. Another important concern was overexpression of DPP4, which might lead to diabetes, accordingly the work was also performed for the regulation of the DPP4 gene expression. In this regard, three types of Anderson promoters (strong, moderate and weak) were utilized to study the control overexpression. This is the first report of idealistic trial for control the exogenous DPP4 gene-expression by molecular biologic tools.

The Experiences of Health Care Workers during the COVID-19 Pandemic in Korea: a Qualitative Study

Lee Ju-Yeon,Lee Ji-yeon,Lee So Hee,Kim Jangrae,Park Hye Yoon,Kim Yeonjae,Kwon Ki Tae 대한의학회 2021 Journal of Korean medical science Vol.36 No.23

Background: During the coronavirus disease 2019 (COVID-19) pandemic, health care workers (HCWs) have faced multiple physical and psychological challenges while carrying out their duties. In this study, we examined the experiences of specific groups of HCWs during the pandemic. Methods: From 18 November to 30 December 2020, we conducted a qualitative study using semi-structured, face-to-face interviews with four groups of 14 HCWs in three cities (Seoul, Daegu, and Gwangju) in South Korea. The HCWs who participated in the focus groups included physicians, nurses, medical practitioners, and cleaning staff who directly or indirectly cared for patients during the COVID-19 epidemic. Interviews were transcribed verbatim and analyzed using the consensual qualitative research approach. Results: Our qualitative data analysis revealed four main domains: work-related struggles, personal life-related struggles, psychological stress, and health-related struggles. Health care providers were challenged by working in critical situations and were overwhelmed by heavy workloads, fear of infection, lifestyle changes, and psychological and physical struggles. Conclusion: Our findings could serve as a foundation for establishing health care systems and policies that help HCWs cope with occupational stress, thus increasing their ability to adapt to the ongoing COVID-19 pandemic.

Expert Consensus on Measures to Promote Physical and Psychological Health among COVID-19-Related Healthcare Workers in Korea using Delphi Technique

Lee So Hee,Noh Jin-Won,Kim Yeonjae,Ryoo Hyun Wook,Park Kyung-Hwa,Park Kyung-Hwa,Lee Shinwon,Cheong Hae Suk,Heo Sang Taek,Hong Ki Jeong,Kim Kyoung-Beom,Kwon Ki Tae 대한감염학회 2022 Infection and Chemotherapy Vol.54 No.2

Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health. Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health.

Expression Study of a Recombinant Plasmid containing Dipeptidyl Peptidase-4 Gene in E. coli : A Plausible Application for Celiac Disease Patients to Digest Gluten

Yeonjae Lee,Ryan Kang,Jenna Kwon,Kyuhee Jo,Jungbin Im,Sangwook Jung,DongHyun Lee,Juhyeon Lee,Jeong-Sang Lee 한국인터넷방송통신학회 2018 Journal of Advanced Smart Convergence Vol.7 No.2

Celiac disease (CD) is an immune-mediated enteropathy of small intestine diagnosed in both childhood and adulthood. CD is caused by gluten, which produces gliadorphin during its digestion. The enzyme dipeptidyl peptidase-4 (DPP4) breaks gliadorphin down nevertheless the last tripeptide remains and eventually inhibits DPP4, thus slowing down its process. Therefore, the idea is to produce an additional DPP4 enzyme which is crucial. Consequently, the functional DPP4 gene was cloned into pCDNA3 intermediate (FLAG+DPP4) vector and finally a recombinant plasmid pSB1C3 (Andersons promoters+ FLAG+DPP4) was constructed using synthetic biology. Normally, a DPP4 inhibitor is used as a cure for diabetes. Another important concern was overexpression of DPP4, which might lead to diabetes, accordingly the work was also performed for the regulation of the DPP4 gene expression. In this regard, three types of Anderson promoters (strong, moderate and weak) were utilized to study the control overexpression. This is the first report of idealistic trial for control the exogenous DPP4 gene-expression by molecular biologic tools.

Possibility of Decreasing Incidence of Human Immunodeficiency Virus Infection in Korea

Lee Minkyeong,Park Wan Beom,Kim Eu Suk,Kim Yeonjae,Park Sang-Won,Lee Eunyoung,Oh Myoung-don,Kim Nam Joong,Kim Hong Bin,Song Kyoung-Ho,Choe Pyoeng Gyun,Kang Chang Kyung,Lee Chan Mi,Choi Yunsang,Moon So 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.4

Background: The number of newly diagnosed cases of human immunodeficiency virus (HIV) infection in Korea, which had increased until 2019, has markedly decreased since the coronavirus disease 2019 pandemic started. This study evaluated whether the decrease is due to a reduction in the incidence of HIV infection and/or delayed diagnosis during the pandemic. Materials and Methods: We reviewed the medical records of 587 newly diagnosed patients with HIV infection between February 2018 and January 2022 from four general hospitals, and their characteristics were compared between the prepandemic and pandemic periods. The lapse time from infection to diagnosis was estimated using an HIV modeling tool. Results: The estimated mean times to diagnosis were 5.68 years (95% confidence interval [CI]: 4.45 - 6.51 years) and 5.41 years (95% CI: 4.09 - 7.03 years) before and during the pandemic, respectively (P = 0.016). The proportion of patients with acquired immunodeficiency syndrome-defining illnesses, expected to visit hospitals regardless of the pandemic, decreased from 17.2% before the pandemic to 11.9% during the pandemic (P = 0.086). Conclusion: The decrease in the number of newly diagnosed cases of HIV infection in Korea might have resulted from an actual decrease in the incidence of HIV infection rather than a worsening of underdiagnosis or delayed diagnosis.

1980년대 한국 여성주의 형성과 교차성 개념을 통해 본 김인순의 페미니즘 작업: 한국 페미니즘 미술사 지형도 다시 그리기

이연재(Lee, Yeonjae) 현대미술사학회 2020 현대미술사연구 Vol.0 No.48

본 논문은 여성의 다름에 주목하는 현대의 페미니즘 관점에서 우리나라 여성학계 및 여성운동의 형성과 발맞추어 나간 김인순의 페미니즘 작업을 다시 읽어보려는 시도이다. 한국 페미니즘 미술사가 본격적으로 논의되던 1990년대에 포스트모더니즘 이론을 미술비평 방법론으로 사용하는 과정에서 1980년대 한국의 사회정치적 상황과 한국 여성주의의 발전에는 무관심한 채로 김인순과 민족미술협회 여성분과의 작업은 여성성과 여성미학이 결여된, 민중미술 흐름의 일부로 번역되었다. 여성을 이중으로 억압하는 현실이 바로잡히길 갈망했던 김인순의 작업을 단지‘ 민중미술의 일부로서의 여성미술’로만 해석하는 시각은 그 안에 담긴 페미니즘 인식을 가리어 결국 여성미학이 결여된 정치투쟁적 미술로서 받아들이게 만든다. 우리는 페미니즘의 다양하고 동등한 이론들을 빌려와 우리 사고의 균형 감각을 되찾고, 소외-지배 이데올로기와 결별하는 방식으로 한국 페미니즘 미술사 지형도를 다시 그릴 수 있다. 본고에서는‘ 여성의 다름’과‘ 한국 여성이라는 공통점’의 간극을 조정하기 위해, 양극을 화해시키는 방법으로써 김인순의 작업을 읽어보려 한다. 성·계급·민족의 문제를 교차하는 방식으로 김인순의 작품을 다시 읽어나가면 이것이 한국의 역사적·시대적 맥락 및 사회·경제적 계급 속에 위치한 여성의 삶과 한국 여성의 페미니즘 인식에 관한 것이라는 점이 드러날 것이다. This study is an attempt to re-read Kim In-soon’s feminist work in line with the formation of Korean women’s academia and the Korean women’s movement from the perspective of modern feminism, which focuses on women’s differences. In the 1990s, when Korean feminist art history was debated in earnest with post-structuralist theory as an art criticism methodology, the socio-political situation in Korea and the development of Korean feminism in the 1980s were ignored. As a result, the works of Kim In-soon and group works of The Women’s Division of The Korean People’s Artists Association were translated as part of the flow of People’s Art (Minjung Art) in Korea, lacking femininity and feminine aesthetics. The interpretation of the work of Kim In-soon, who longed for the reality of the double oppression of women to be corrected, as merely ‘female art as a part of Minjung Art’ obscures the feminist perception it contains and reduces it to agitative art lacking feminine aesthetics. We will be able to restore a sense of balance in our thinking while borrowing various and equal theories of feminism and redraw the map of Korean feminist art history in a way that breaks with the alienation-dominant ideology. So, to adjust the gap between ‘the difference between women’ and ‘the common point of Korean women’, I will reread Kim In-soon’s work as a way to reconcile the two sides. Rereading it in a way that intersects sex, class, and ethnicity reveals that Kim’s work is about the life of women and the perception of feminism of Korean women located in the historical context of Korea and socio-economic class.

Malware에 감염된 Endpoint환경에서 안전한 전자금융거래

이연재 ( Yeonjae Lee ),이희조 ( Heejo Lee ) 한국정보처리학회 2014 한국정보처리학회 학술대회논문집 Vol.21 No.2

유무선 인터넷이 보편화되고 이용이 확산되면서 금융권에서는 고객의 편의성 증진을 위해 영업점의 상당한 업무를 인터넷뱅킹과 모바일뱅킹 등을 이용하여 처리할 수 있는 IT환경을 제공하고 있다. 이러한 Endpoint 환경의 변화는 점점 더 지능화되고 있는 사이버 공격 기술로 보안 위협이 증대되고 있는 실정이다. 이를 해결하기 위한 방법 중의 하나로 본 연구에서는 Reverse sandboxing 기술과 화이트리스트 기반의 보안 기술이 내장된 커널 수준의 TSX(Trusted Security extension)기술을 통하여 맬웨어가 감염된 상태에서도 안전하게 전자금융거래를 할 수 있는 Endpoint환경을 제공한다.

FMEA 와 FTA 를 활용한 챗봇 시스템의 사용성 개선 프로세스

이연재 ( Yeonjae Lee ),송재우 ( Jaewoo Song ),한혁수 ( Hyuksoo Han ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

챗봇(Chatbot)은 자연어처리기술 등 인공지능 기술을 기반으로 한 사용자 친화적인 대화 방식 인터페이스를 제공하는 장점이 있어, 금융, 상담, 주문 등 다양한 산업 분야에서 적용되고 있다. 그러나, 챗봇의 응답이 사용자의 정신 모형과 불일치하는 경우, 다음 대화를 이어가는데 어려움을 야기하게 된다. 그러므로, 챗봇의 사용성을 확보하기 위해서는 응답 오류의 제거 또는 완화가 필수적이다. 기존의 챗봇의 사용성 개선과 관련된 연구들은 설문조사와 인터뷰 등 사용성 평가를 통해 상위 수준의 개선 방향만을 제안하고 있다. 따라서, 챗봇 개발 시, 실무자들이 응답 오류의 문제점을 분석하고, 이를 해결하기 위한 구체적인 개선 방안을 제시하는 데 한계가 있었다. 본 논문에서는 FMEA(Failure Modes and Effects Analysis) 기법을 활용해, 응답 오류의 치명도를 파악하고, 치명적인 오류들에 대해서는 FTA(Fault Tree Analysis) 기법을 기반으로 원인 분석을 실시하여 구체적으로 문제를 해결하기 위한 프로세스를 제안한다. 본 프로세스의 효용성을 검증하기 위해 주문 도메인의 챗봇에 적용해 보았다.

중도 지적장애인의 다면적 평가를 위한 ICF Core Set 개발: 현장전문가 면담을 통한 항목 추출을 중심으로

이연재(Lee, Yeonjae),임혜경(Lim Hyegyeoung) 부산대학교 교육발전연구소 2019 교육혁신연구 Vol.29 No.1

연구목적: 본 연구의 목적은 중도 지적장애인의 다면적 평가를 위한 ICF Core Set을 개발하는 것으로 현장전문가와의 면담을 통해 4개 영역의 항목을 추출하는 데 있다. 연구방법: 연구 참여자는 중도 지적장애인을 5년 이상 지도한 경험이 있는 현장전문가 10명이다. 면담 기간은 2017년 9월에서 11월까지 약 2개월이 소요되었다. 면담 질문지는 WHO(2001)가 공표한 ICF 분류체계의 신체기능 및 신체구조, 활동과 참여, 환경요인의 4개 영역에 대한 중도 지적장애인의 특성, 능력과 수행 정도, 지원의 정도를 알아보는 내용으로 구성하였다. 면담자료는 연구 참여자의 동의를 구해 모두 녹음한 후 필사본으로 전사한 다음 4개 영역으로 유형화하였다. 연구결과: 중도 지적장애인의 다면적 평가를 위한 ICF Core Set 개발에 포함되어야 할 항목으로 총 149개가 추출되었다. 영역별로는 신체기능이 57개 항목, 신체구조는 11개 항목, 활동과 참여는 51개 항목, 환경요인은 30개 항목이 각각 추출되었다. 논의 및 결론: 향후 본 연구에서 추출한 항목의 중요도를 평정하기 위하여 2차에 걸친 델파이 조사를 실시하여 중도 지적장애인의 다면적 평가를 위한 포괄형 및 단축형 ICF Core Set을 개발할 것이다. Purpose: The purpose of this study was to identify evaluation items through interviews with field experts in order to develop ICF Core Set for multidisciplinary evaluation of persons with severe intellectual disabilities. Method: The study participants were 10 field experts with more than five years of experience instructing persons with severe intellectual disabilities. The interview period took about two months from September to November 2017. The interview questionnaire was designed to understand the abilities and performance of persons with severe intellectual disabilities in the 4 areas(body function and structure, activities and participation, environmental factors, contextual factors) of the ICF system suggested by WHO(2001). The interview data were recorded and transferred to the transcripts with the consent of study participants, and the data were categorized into 4 areas under triangulation method. Results: A total of 149 items were extracted to be included in the development of the ICF Core Set for the multidisciplinary evaluation of persons with severe intellectual disabilities. There were 57 items on body functions, 11 items on body structures, 51 items on activities and participation, 30 items on environmental factors. Conclusion: Hereafter, a two-phase Delphi survey will be conducted to assess the importance of items extracted from this study, while developing a comprehensive and shortened version of ICF Core Set for multidisciplinary evaluation of persons with severe intellectual disabilities.

여행 리뷰 분석 사이트

이연재(YeonJae Lee),이승희(Seunghee Lee),안병구(Beongku An) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.11