Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

Crystal structure of xenotropic murine leukaemia virus-related virus (XMRV) ribonuclease H

Kim, Ju ,Hee,Kang, Sunghyun,Jung, Suk-Kyeong,Yu, Keum ,Ran,Chung, Sang ,J.,Chung, Bong ,Hyun,Erikson, Raymond ,L.,Kim, Bo ,Yeon,Kim, Seung ,Jun Portland Press Ltd. 2012 Bioscience reports Vol.32 No.5

<P>RNase H (retroviral ribonuclease H) cleaves the phosphate backbone of the RNA template within an RNA/DNA hybrid to complete the synthesis of double-stranded viral DNA. In the present study we have determined the complete structure of the RNase H domain from XMRV (xenotropic murine leukaemia virus-related virus) RT (reverse transcriptase). The basic protrusion motif of the XMRV RNase H domain is folded as a short helix and an adjacent highly bent loop. Structural superposition and subsequent mutagenesis experiments suggest that the basic protrusion motif plays a role in direct binding to the major groove in RNA/DNA hybrid, as well as in establishing the co-ordination among modules in RT necessary for proper function.</P>

Differentiation of Dopaminergic Neurons from Mesenchymal-Like Stem Cells Derived from Human Umbilical Cord Vein

Kim Ju-Ran,Lee Jin-Ha,Jalin Anjela Melinda,Lee Chae-Yeon,Kang Ah-Reum,Do Byung-Rok,Kim Hea-Kwon,Kam Kyung-Yoon,Kang Sung-Goo 한국발생생물학회 2009 발생과 생식 Vol.13 No.3

One of the most extensively studied populations of multipotent adult stem cells are mesenchymal stem cells (MSCs). MSCs derived from the human umbilical cord vein (HUC-MSCs) are morphologically and immunophenotypically similar to MSCs isolated from bone marrow. HUC-MSCs are multipotent stem cells, differ from hematopoietic stem cells and can be differentiated into neural cells. Since neural tissue has limited intrinsic capacity of repair after injury, the identification of alternate sources of neural stem cells has broad clinical potential. We isolated mesenchymal-like stem cells from the human umbilical cord vein, and studied transdifferentiation-promoting conditions in neural cells. Dopaminergic neuronal differentiation of HUC-MSCs was also studied. Neural differentiation was induced by adding bFGF, EGF, dimethyl sulfoxide (DMSO) and butylated hydroxyanisole (BHA) in N2 medium and N2 supplement. The immunoreactive cells for -tubulin III, a neuron-specific marker, GFAP, an astrocyte marker, or Gal-C, an oligodendrocyte marker, were found. HUC-MSCs treated with bFGF, SHH and FGF8 were differentiated into dopaminergic neurons that were immunopositive for tyrosine hydroxylase (TH) antibody. HUC-MSCs treated with DMSO and BHA rapidly showed the morphology of multipolar neurons. Both immunocytochemistry and RT-PCR analysis indicated that the expression of a number of neural markers including NeuroD1, -tubulin III, GFAP and nestin was markedly elevated during this acute differentiation. While the stem cell markers such as SCF, C-kit, and Stat-3 were not expressed after neural differentiation, we confirmed the differentiation of dopaminergic neurons by TH/-tubulin III positive cells. In conclusion, HUC-MSCs can be differentiated into dopaminergic neurons and these findings suggest that HUC-MSCs are alternative cell source of therapeutic treatment for neurodegenerative diseases.

Miniscalpel acupuncture points of the cervical region: A review

Kim, Ju-ran,Lee, Jung Hee,Gong, Han Mi,Jun, Seungah,Chung, Yeon-Joong,Park, Chung A,Choi, Seong Hun,Lee, Geon-Mok,Lee, Hyun-Jong,Kim, Jae Soo Korean AcupunctureMoxibustion Medicine Society 2017 대한침구의학회지 Vol.34 No.3

Objectives : We conducted a literature search to identify trends with respect to miniscalpel acupuncture points (MAPs) of the cervical region with the aim of providing guidance regarding miniscalpel acupuncture (MA) in the cervical region. Methods : We searched 2 overseas sites (PubMed and Google Scholar) and 5 domestic sites (NDSL, RISS, KISS, OASIS and Korean Traditional Knowledge Portal) for clinical trials of MA of the cervical region. The search terms used were "acupotomy or miniscalpel acupuncture or miniscalpel" and "cervical." Of 73 studies found, 11 were included in this review, 6 of which presented exact MAPs. Owing to the lack of data, we refer to 1 Korean and 4 Chinese articles to analyze the exact location and meaning of the MAPs used in the published studies and to propose other clinically applicable MAPs. Results : We identified 10 of the most commonly used MAPs in the published studies, including those located below the external occipital protuberance, 2-2.5 cm both side down of the external occipital protuberance, 4.5-5 cm both side down of the external occipital protuberance and C3-7 spinous process. Furthermore, we proposed 13 additional MAPs that can be used clinically. Conclusion : We hope that this study will form a basis for the development of MA and hope to improve the safety and reproducibility in future MA studies of the cervical region.

Effectiveness of Home Visiting Treatment using Traditional Korean Medicine

Kim, Na-Young,Lee, Jae-Hoon,Go, Ho-Yeon,Youn, Sang-Jun,Lee, Jae-Hyok,Lee, Dong-Nyoung,Shin, Mi-Ran The Society of Korean Medicine 2011 대한한의학회지 Vol.32 No.6

Objectives: The study was investigated to assess efficacy for home visiting treatment using Traditional Korean Medicine (TKM). Methods: The traditional medical doctor of Um-Sung health center has treated the 109 patients and they also answered the questionnaires of home visiting treatment using TKM before and after. The questionnaires were composed of flexibility, pain, health behavior, perception of health, satisfaction, cognition of TKM, etc. The questionnaires were analyzed by use of paired t-test and chi-square test. Results: They have significantly improved the flexibility (p<.001), pain (p<.001), health behavior (p<.001), and the perception (p<.001) of health after home visiting treatment. It was also brought to improve about the perception of TKM, and showed a good satisfaction and the improvement of health. Conclusion: Home visiting treatment using TKM is helpful to improve the health based on these results. We need to systemize the research for the activation of home visiting treatment using TKM.

중등교사들의 환경교육 수업에 대한 교사 전문성 분석

Yeon-A Son(손연아),Soojeong Myeong(명수정),Don-Hyung Choi(최돈형),Byeong-Mee Min(민병미),Kyoung-Ran Kim(김경란) 한국환경교육학회 2009 環境 敎育 Vol.22 No.2

이 연구의 목적은 중등교사들의 환경 관련 수업 전문성을 심층적으로 분석하는데 있다. 연구를 진행하기 위해 먼저, 서울과 경기도의 중등교사 431명을 대상으로 환경수업 전문성에 대한 자기평가를 실시하였다. 자기평가도구로는 Hungerford 등(1994)이 개발한 ‘환경교육자가 갖추어야할 능력 범주 기준’를 활용하였다. 그리고 자기평가를 실시한 중등교사들 중에서 2명의 교사가 진행하는 환경 관련 수업을 실제 참관하여 비디오녹화 분석하였고, 수업교사들과의 면담도 실시하였다. 환경 관련 수업 전문성에 대한 자기평가 결과, ‘생태학적 기초’ 영역이 평균 2.80, 개념적 인식 영역이 평균 2.86, 조사와 평가 영역이 평균 2.83, 환경적 행위기능 영역이 평균 2.94, 교육적 적용 영역이 평균 2.84로 나타났다. 또한, 실제 수업을 분석한 결과, 환경관련 수업을 진행한 교사들은 현대 환경교육에서 강조되고 있는, ‘생태학적 지식을 교수학습에 효과적으로 적용하는 측면’, ‘환경적 의사결정을 위해 개인적 가치를 명료화하는 측면’, ‘환경쟁점과 해결책에 대한 자신의 가치관을 평가하는 측면’, ‘환경적으로 지속가능발전을 위해 시민적 행위기능을 개발하는 측면’에서 다소 미흡한 것으로 나타났다. 이 연구는 중등학교에서 환경관련 수업을 담당하고 있는 교사들을 위한 환경교육 연수프로그램을 개발하고 운영하는데 의미 있는 시사점을 제공해 줄 수 있을 것이다. The objective of this study is to find out the professionalism of secondary school teachers in teaching environmental subjects. This research was conducted using a questionnaire survey of 431 secondary school teachers from 34 schools in Seoul and Gyeonggi Province, Korea. A 5-point Likert scale self-evaluation questionnaire on their professionalism in environmental education was developed based on Hungerford et al.'s (1994) evaluation criteria. Two middle school teachers' classes were observed, and video taping and interviewing were also conducted. The survey analysis revealed that teachers evaluated their professionalism on environmental subjects to be a little above the middle of the scale. The mean values were 2.80 for the foundation level; 2.86 for the conceptual awareness level; 2.84 for the investigation level; 2.94 for the environmental action skills level; and 2.84 for the educational application level. However, class observations revealed that there was some gap between their self-evaluation of professionalism and their actual classroom application. Both classes were not that satisfactory with regard to 'the abilities to communicate and apply ecological concepts', 'the ability to clarify the roles of different value systems for environmental issues and personal values in decision making', 'the ability to clarify their own value system regarding solutions to environmental issues', and 'the ability to apply citizen action skills for solving environmental issues and for sustainable development'. The results provide a basic reference for the improvement of environmental-related education.

Annual Case Volume and One-Year Mortality for Endovascular Treatment in Acute Ischemic Stroke

Kim Jun Yup,Kang Jihoon,Kim Beom Joon,Kim Seong-Eun,Kim Do Yeon,Lee Keon-Joo,Park Hong-Kyun,Cho Yong-Jin,박종무,Lee Kyung Bok,Cha Jae-Kwan,Lee Ji Sung,Lee Juneyoung,Yang Ki Hwa,Hong Ock Ran,Shin Ji Hyeon 대한의학회 2022 Journal of Korean medical science Vol.37 No.36

Background: The association between endovascular treatment (EVT) case volume per hospital and clinical outcomes has been reported, but the exact volume threshold has not been determined. This study aimed to examine the case volume threshold in this context. Methods: National audit data on the quality of acute stroke care in patients admitted via emergency department, within 7 days of onset, in hospitals that treated ≥ 10 stroke cases during the audit period were analyzed. Ischemic stroke cases treated with EVT during the last three audits (2013, 2014, and 2016) were selected for the analysis. Annual EVT case volume per hospital was estimated and analyzed as a continuous and a categorical variable (in quartiles). The primary outcome measure was 1-year mortality as a surrogate of 3-month functional outcome. As post-hoc sensitivity analysis, replication of the study results was examined using the 2018 audit data. Results: We analyzed 1,746 ischemic stroke cases treated with EVT in 120 acute care hospitals. The median annual EVT case volume was 12.0 cases per hospital, and mortality rates at 1 month, 3 months, and 1 year were 12.7%, 16.6%, and 23.3%, respectively. Q3 and Q4 had 33% lower odds of 1-year mortality than Q1. Adjustments were made for predetermined confounders. Annual EVT case volume cut-off value for 1-year mortality was 15 cases per year (P < 0.02). The same cut-off value was replicated in the sensitivity analysis. Conclusion: Annual EVT case volume was associated with 1-year mortality. The volume threshold per hospital was 15 cases per year.

Reversible Regulation of Enzyme Activity by pH-Responsive Encapsulation in DNA Nanocages

Kim, Seong Ho,Kim, Kyoung-Ran,Ahn, Dae-Ro,Lee, Ji Eun,Yang, Eun Gyeong,Kim, So Yeon American Chemical Society 2017 ACS NANO Vol.11 No.9

<P>Reversible regulation of enzyme activity by chemical and physical stimuli is often achieved by incorporating stimuli-responsive domains in the enzyme of interest. However, this method is suitable for a limited number of enzymes with well-defined structural and conformational changes. In this study, we present a method to encapsulate enzymes in a DNA cage that could transform its conformation depending on the pH, allowing reversible control of the accessibility of the enzyme to the surrounding environment. This enabled us to regulate various properties of the enzyme, such as its resistance to protease-dependent degradation, binding affinity to the corresponding antibody, and most importantly, enzyme activity. Considering that the size and pH responsiveness of the DNA cage can be easily adjusted by the DNA length and sequence, our method provides a broad impact platform for controlling enzyme functions without modifying the enzyme of interest.</P>

Relative Effectiveness of COVID-19 Vaccination in Healthcare Workers: 3-Dose Versus 2-Dose Vaccination

Kim Sung Ran,Kang Hyeon Jeong,Jeong Hye Rin,Jang Su Yeon,Lee Jae Eun,Kim Da Eun,Lee Hae Ry,Cho Min Hee,Noh Ji Yun,Cheong Hee Jin,Kim Woo Joo,Song Joon Young 대한의학회 2022 Journal of Korean medical science Vol.37 No.35

The omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to have high infectivity and is more likely to evade vaccine immunity. However, booster vaccination is expected to strengthen cross-reactive immunity, thereby increasing the vaccine effectiveness (VE). This study aimed to evaluate the relative VE of the 3-dose (booster) vaccination compared with the 2-dose primary series vaccination in healthcare workers during omicron variant-dominant periods. During the omicron-dominant period from February 1, 2022 to February 28, 2022, a 1:1 matched case-control study was conducted. Healthcare workers with positive SARS-CoV-2 test results were classified as positive cases, whereas those with negative results served as controls. Compared with the 2-dose primary series vaccination, booster vaccination with mRNA vaccine showed moderate VE (53.1%). However, in multivariate analysis including the time elapsed after vaccination, the significant VE disappeared, reflecting the impact of recent vaccination rather than the third dose itself.

Korean Society of Infectious Diseases/National Evidence-based Healthcare Collaborating Agency Recommendations for Anti-SARS-CoV-2 Monoclonal Antibody Treatment of Patients with COVID-19

Kim Sun Bean,Kim Jimin,Huh Kyungmin,Choi Won Suk,Kim Yae-Jean,Joo Eun-Jeong,Kim Youn Jeong,Yoon Young Kyung,Heo Jung Yeon,Seo Yu Bin,Jeong Su Jin,Yu Su-Yeon,Peck Kyong Ran,Choi Miyoung,Yeom Joon Sup 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.2

Neutralizing antibodies targeted at the receptor-binding domain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein have been developed and now under evaluation in clinical trials. The US Food and Drug Administration currently issued emergency use authorizations for neutralizing monoclonal antibodies in non-hospitalized patients with mild to moderate coronavirus disease 2019 (COVID-19) who are at high risk for progressing to severe disease and/or hospitalization. In terms of this situation, there is an urgent need to investigate the clinical aspects and to develop strategies to deploy them effectively in clinical practice. Here we provide guidance for the use of anti-SARS-CoV-2 monoclonal antibodies for the treatment of COVID-19 based on the latest evidence.