강제환우와 보존기간이 난질에 미치는 영향

오홍록,이봉덕,이수기,류현덕,유동조 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.1

An experiment was conducted to investigate the effects of forced molting and egg storage time on the various egg qualities. A total of 240 ISA Brown layers (60 wk of age) were employed as the unmolted treatment (Control). Two hundred and forty ISA Brown layers, molted at the age of 55 wk, were used as a forced molting treatment (T1), and the same number and strain of layers, molted at the age of 70 wk. were also used as the another forced molting treatment (T2). A total of 120 eggs were sampled from each treatment, and divided into six sets, 20 eggs per set. These six sets were stored for 1, 3, 6, 9, 12, and 15 days at 18℃ temperature, respectively. Eggs from T1 were collected from laying hens at the age of 68 wk, which started molting at 60 wk of age and achieved 50% egg production at 63 wk of age. Eggs from T2 were collected from hens at 82 wk of age, which started molting at 70 wk of age and achieved 50% egg production at 78 wk of age. The eggshell strength of T1 was significantly (p<0.05) higher than the Control and T2, and the storing periods did not affect the eggshell strength at all. Neither the forced molting nor the storing periods did not exert any consistent effect on the egg weight, eggshell thickness, eggshell color and egg yolk color. The albumin heights of T1 and T2 were significantly (p<0.05) lower than the Control, and it was remarkably reduced gradually as the storage periods increased in all three treatments. The Haugh unit showed very similar trends as the albumin height, indicating that both albumin height and Haugh unit were very much related to each other. In conclusion, the forced molting improves the eggshell strength, but decreases the albumin height and Haugh unit. The storage of eggs also decreases the albumin height and Haugh unit regardless of molting.

Sperm-mediated Gene Transfer in the Chinese Honeybee, Apis cerana cerana (Hymenoptera: Apidae)

Dong-Sheng Guo,Liang-Xian Sun,Zhi-Jiang Zeng,Xian-Bing Xie 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.4

Transgenic Apis cerana cerana were produced by sperm-mediated gene transfer (SMGT). In the experiment, the foreign DNA was linearized and introduced with sperm during the instrumental insemination of virgin queen. The descendants of the experimental colonies were analyzed. In the green fluorescent-positive offspring, green fluorescence was observed for 1- to 2-day-old larvae, the predicted fragment was isolated by means of PCR amplification of genomic DNA and the expression of transferred genes was confirmed at transcriptional level by reverse transcription-polymerase chain reaction. These results showed that the exogenous gene could be integrated in a fraction of the germ line cells of the queen Apis cerana cerana and transmitted to offspring by SMGT.

Common Variations of DNA Repair Genes are Associated with Response to Platinum-based Chemotherapy in NSCLCs

Li, Xian-Dong,Han, Ji-Chang,Zhang, Yi-Jie,Li, Hong-Bing,Wu, Xue-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Aim: Individual differences in chemosensitivity and clinical outcome of non-small-cell lung cancer (NSCLC) patients may be induced by host inherited factors. We investigated the impact of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln gene polymorphisms on the efficacy of platinum-based chemotherapy in NSCLC patients. Methods: A total of 496 were consecutively selected from the Affiliated Hospital of Nantong University between Jan. 2003 and Nov. 2006, and all patients were followed-up until Nov. 2011. The genotyping of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln was conducted by duplex polymerase-chain-reaction with the confronting-two-pair primer methods. Results: Individuals with XPD 312 C/T+T/T and XPD 711 C/T+T/T exhibited poor responses to chemotherapy when compared with the wild-type genotype, with adjusted ORs(95% CI) of 0.67(0.38-0.97) and 0.54(0.35-0.96), respectively. Cox regression showed the median PFS and OS of patients of XPD 312 C/T+T/T genotype and XPD 711 C/T+T/T genotype to be significantly lower than those with wild-type homozygous genotype. Conclusion: We found polymorphisms in XPD to be associated with response to platinum-based chemotherapy in NSCLC, and our findings provide information for therapeutic decisions for individualized therapy.

Sperm-mediated Gene Transfer in the Chinese Honeybee, Apis cerana cerana (Hymenoptera: Apidae)

Guo, Dong-Sheng,Sun, Liang-Xian,Zeng, Zhi-Jiang,Xie, Xian-Bing Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.4

Transgenic Apis cerana cerana were produced by sperm-mediated gene transfer (SMGT). In the experiment, the foreign DNA was linearized and introduced with sperm during the instrumental insemination of virgin queen. The descendants of the experimental colonies were analyzed. In the green fluorescent-positive offspring, green fluorescence was observed for 1- to 2-day-old larvae, the predicted fragment was isolated by means of PCR amplification of genomic DNA and the expression of transferred genes was conferred at transcriptional level by reverse transcription-polymerase chain reaction. These results showed that the exogenous gene could be integrated in a traction of the germ line cells of the queen Apis cerana cerana and transmitted to offspring by SMGT.

민적사약(民的私約)의 법령화에 대한 고찰

이현동 ( Xian Dong Li ),이조군 ( Zhao Jun Li ) 영남대학교 법학연구소 2007 영남법학 Vol.24 No.-

Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

Liao, Xian-Dong,Zhou, Chang-Hai,Zhang, Jing,Shen, Jing-Lin,Wang, Ya-Jing,Jin, Yong-Cheng,Li, Sheng-Li Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. Methods: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. Results: In MAC-T cells, ATRA increased the mRNA levels of α_S1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA. Conclusion: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes.

Rethinking China-ROK Strategic Cooperative Partnership Relation

Han Xian-dong(한셴둥) 한국전략문제연구소 2013 전략연구 Vol.60 No.-

전략적 동반자 관계란 공통의 전략적 목표와 이해관계를 갖고 있고 주요 국제문제에 대해서도 동일하거나 비슷한 시각을 갖기 때문에 상대적으로 오랫동안 밀접하고 안정적인 협력을 유지하는 상황으로 해석될 수 있다. 저자는 전략적 동반자 관계의 특징을 다음과 같이 보고 있다. (1) 장기간의 안정성 (2) 상위정치(군사?정치 문제에 한하여 무역?경제 문제는 다루지 않는 외교 방침) (3) 다양한 수준이 바로 그것이다. 더욱이 두 국가 사이에서 전략적 동반자 관계는 특정 조건 아래서만 성립될 수 있다. 1990년대 이후 빠른 경제협력과 인적 교류를 기반으로 한중간에 전략적 협력이 형성됐다. 하지만 한반도 문제에 대한 전략적 목표의 인식 차이로 한중 전략적 협력과 동맹관계는 여전히 취약하며, 실체화시킬 필요가 있다. 현재로선 서로간 경제적 상호의존 관계를 안정화시키고 인적교류를 확대하며 향후 정책과 안보에 대한 상호이해를 공유하는 것이 매우 중요하다. Strategic partnership can be defined as a situation of maintaining a stable cooperation by relevant states for a relatively long period because they have common strategic objectives and interests, and they hold same or similar views about some major international issues. From the author"s perspective, the relation of strategic partnership should own the following three features: (1) long- term stability, (2) high politics and (3) multiple levels. Moreover, only under certain conditions is it possible that the strategic partnership could be established between two states. The rapid development in such fields as economic cooperation and humanities exchange between China and ROK since 1990s has laid the basic groundwork for China-ROK strategic cooperation and partnership. However, because there are some differences between their strategic goals towards the Korean Peninsula, China-ROK relation of strategic cooperation and partnership is still fragile and needs to be substantialized. At present, it is very important to stabilize both sides" economic interdependence, expand humanities exchange and further mutual understanding on politics and security.

Dynamic Compliance and its Compensation Control of HIVC Force Control System

Kai-xian Ba,Bin Yu,Wen-feng Li,Dong-kun Wang,Ya-liang Liu,Guo-liang Ma,Xiang-dong Kong 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.2

In this paper, the dynamic compliance and its compensation control of the force control system on the highly integrated valve-controlled cylinder (HIVC), the joint driver of the hydraulic drive legged robot, is researched. During the robot motion process, the outer loop dynamic compliance control is applied on the base of hydraulic control inner loop and most inner loop control are the force or torque closed loop control. While the dynamic compliance control effectiveness of outer loop can be affected by the inner loop self-dynamic-compliance. Based on this problem, the dynamic compliance series composition theory of HIVC force control system as well as the analysis of its self-dynamiccompliance is proposed. And then the paper comes up with the compliance-enhanced control, which is a compound compensation control method of dynamic compliance with multiple series branches. Finally, the experiment results indicate that the control method mentioned above can enhance the dynamic compliance of HIVC force control system observably. This provides the compensation control method of inner loop dynamic compliance for the outer loop compliance control requiring the high accuracy and high robustness for the robot.

Dynamic Compliance and its Compensation Control of HIVC Force Control System

Ba, Kai-xian,Yu, Bin,Li, Wen-feng,Wang, Dong-kun,Liu, Ya-liang,Ma, Guo-liang,Kong, Xiang-dong The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.2

In this paper, the dynamic compliance and its compensation control of the force control system on the highly integrated valve-controlled cylinder (HIVC), the joint driver of the hydraulic drive legged robot, is researched. During the robot motion process, the outer loop dynamic compliance control is applied on the base of hydraulic control inner loop and most inner loop control are the force or torque closed loop control. While the dynamic compliance control effectiveness of outer loop can be affected by the inner loop self-dynamic-compliance. Based on this problem, the dynamic compliance series composition theory of HIVC force control system as well as the analysis of its self-dynamic-compliance is proposed. And then the paper comes up with the compliance-enhanced control, which is a compound compensation control method of dynamic compliance with multiple series branches. Finally, the experiment results indicate that the control method mentioned above can enhance the dynamic compliance of HIVC force control system observably. This provides the compensation control method of inner loop dynamic compliance for the outer loop compliance control requiring the high accuracy and high robustness for the robot.

Clinical Significance of Joint Detection of Serum VEGF, SIL-2R and HGF in Patients with Primary Hepatocellular Carcinoma before and after Percutaneous Microwave Coagulation Therapy

Chen, Ji-Dong,Xiong, Yan-Qun,Dong, Ke,Luo, Jun,Yue, Lin-Xian,Chen, Qin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Objective: To investigate the changes of serum vascular endothelial growth factor (VEGF), soluble interleukin-2 receptor (SIL-2R) and hepatocyte growth factor (HGF) contents in patients with primary hepatocellular carcinoma (HCC) before and after percutaneous microwave coagulation therapy (PMCT) and determine their clinical significance. Materials and Methods: Fasting venous blood (3 mL) from 81 patients with primary HCC diagnosed by pathology was collected in the mornings 1 day before PMCT, and 1 day, 7 days and 1 month after PMCT, and then the serum was separated and stored in $-70^{\circ}C$. The contents of VEGF, SIL-2R and HGF were detected by enzyme linked immunosorbent assay (ELISA). Results: The serum VEGF, SIL-2R and HGF contents in 81 patients with primary HCC had obviously dynamic changes before and after PMCT. By comparison to 1 day after PMCT with pre-operation, there was no statistical significance regarding VEGF and SIL-2R contents (P>0.05), but HGF content showed significant difference (P<0.01). Compared with pre-operation, VEGF, SIL-2R and HGF contents 7 days and 1 month after PMCT all manifested significant differences (P<0.01). By comparison to 7 days with 1 month after PMCT, there was no statistical significance regarding the VEGF content (P>0.05), whereas SIL-2R and HGF contents showed significant change (P<0.01). Conclusions: The contents of serum VEGF, SIL-2R and HGF have obviously dynamic changes in primary HCC before and after PMCT, and their joint detection is expected to be an effective hematologic evaluation index of PMCT for primary HCC.