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The p16<sup>INK4a</sup> Antibody Immobilization Method for Immonosensor Application
Yang, Li,Huang, Xian-He,Sun, Liang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12
Background: The $p16^{INK4a}$ is a protein that expressed in Liquid-based cervical cytology specimens and has been proved link to cervical cancer. The $p16^{INK4a}$ could be detection by piezoelectric immunosensor and the immobilization of the $p16^{INK4a}$ antibody influence the sensitivity of the piezoelectric immunosensor. Materials and Methods: $5{\mu}L$ mouse polyclonal antibody against $p16^{INK4a}$ was bound onto the surface of immonosensor through two methods. (directly immobilized method; protein A method). Absorb of the $p16^{INK4a}$ antibody on the surface of immonosensor caused a shift in the resonant frequency of the immunosensor and The frequency changes recorded showed a better reproducibility. The activity of the immobilization antibody with the directly method and protein A method was tested with $p16^{INK4a}$ antigen. Results: The resonant frequency for different antibody immobilization methods were different, and the sensitivity for $p16^{INK4a}$ detection also different. Conclusions: The protein A method was found to be much more better than the directly method for the immobilization of the p16INK4A antibody on the gold electrode of the quartz crystal for cervical lesion detection. The Protein A method created more reproducible and stable immobilization antibody layers with p16INK4A antigen.
Sperm-mediated Gene Transfer in the Chinese Honeybee, Apis cerana cerana (Hymenoptera: Apidae)
Dong-Sheng Guo,Liang-Xian Sun,Zhi-Jiang Zeng,Xian-Bing Xie 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.4
Transgenic Apis cerana cerana were produced by sperm-mediated gene transfer (SMGT). In the experiment, the foreign DNA was linearized and introduced with sperm during the instrumental insemination of virgin queen. The descendants of the experimental colonies were analyzed. In the green fluorescent-positive offspring, green fluorescence was observed for 1- to 2-day-old larvae, the predicted fragment was isolated by means of PCR amplification of genomic DNA and the expression of transferred genes was confirmed at transcriptional level by reverse transcription-polymerase chain reaction. These results showed that the exogenous gene could be integrated in a fraction of the germ line cells of the queen Apis cerana cerana and transmitted to offspring by SMGT.
Sperm-mediated Gene Transfer in the Chinese Honeybee, Apis cerana cerana (Hymenoptera: Apidae)
Guo, Dong-Sheng,Sun, Liang-Xian,Zeng, Zhi-Jiang,Xie, Xian-Bing Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.4
Transgenic Apis cerana cerana were produced by sperm-mediated gene transfer (SMGT). In the experiment, the foreign DNA was linearized and introduced with sperm during the instrumental insemination of virgin queen. The descendants of the experimental colonies were analyzed. In the green fluorescent-positive offspring, green fluorescence was observed for 1- to 2-day-old larvae, the predicted fragment was isolated by means of PCR amplification of genomic DNA and the expression of transferred genes was conferred at transcriptional level by reverse transcription-polymerase chain reaction. These results showed that the exogenous gene could be integrated in a traction of the germ line cells of the queen Apis cerana cerana and transmitted to offspring by SMGT.
MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3
Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.
Clinical Applicability of Multi-Tumor Marker Protein Chips for Diagnosing Ovarian Cancer
Bian, Jing,Li, Bo,Kou, Xian-Juan,Wang, Xu-Na,Sun, Xiao-Xu,Ming, Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Purpose: To assess the value of multi-tumor marker protein chips in the diagnosis and treatment of ovarian cancer. Materials and Methods: Twelve tumor markers (CA19-9, NSE, CEA, CA242, CK19, ${\beta}$-HCG, AFP, SCC, c-PSA, CA125, CA724 and CA15-3) were detected by protein biochip in 220 patients with ovarian carcinomas, 205 with benign ovarian tumors and 200 healthy subjects. Results: The positivity rate was obviously higher in ovarian cancer (77.7%), than that in the benign cases (26.3%, p<0.01) and healthy subjects (4.5%, p<0.01). Serum levels of tumor markers were furthermore significantly higher in cases with lymph node metastasis (86.8%) than those without metastasis (44.7%), p<0.01. Conclusions: Multi-tumor marker protein chips provide important assistance in the diagnosis and treatment evaluation in ovarian cancers.
Proteomic Profiles of Mouse Neuro N2a Cells Infected with Variant Virulence of Rabies Viruses
( Wang Xiao Hu ),( Shou Feng Zhang ),( Cheng Long Sun ),( Zi Guo Yuan ),( Xian Fu Wu ),( Dong Xia Wang ),( Zhuang Ding ),( Rong Liang Hu ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.4
We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using twodimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 θ/δ, and downregulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ≥2 times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS- 11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus`` adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.