Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

정상면역을 가진 성인에서 발생한 식도 방선균증

김현수 ( Hyun Soo Kim ),천종운 ( Jong Woon Cheon ),김민수 ( Min Su Kim ),정창길 ( Chang Kil Jung ),김경록 ( Kyung Rok Kim ),최재원 ( Jae Won Choi ),강동우 ( Dong Woo Kang ),김선영 ( Sun Young Kim ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Hyun Soo Kim, Jong Woon Cheon, Min Su Kim, Chang Kil Jung, Kyung Rok Kim, Jae Won Choi, Dong Woo Kang and Sun Young Kim1 Departments of Internal Medicine and Pathology1, DongKang Medical Center, Ulsan, Korea Actinomycosis is a chronic suppurative disease and caused by Actinomycosis species, principally Actinomyces israelii, which are part of the normal inhabitant on the mucous membrane of the oropharynx, gastrointestinal tract, and urogenital tract. It usually affects cervicofacial, thoracic and abdominal tissue. Cervicofacial type has the highest percentage of occurrence with 50%. Actinomycosis frequently occurs following dental extraction, jaw surgery, chronic infection or poor oral hygiene. It may also be considered as an opportunistic infection in immunocompromised patients such as malignancy, human immunodeficiency virus infection, diabetes mellitus, steroid usage or alcoholism. But, actinomycosis rarely occurs in adults with normal immunity and rare in the esophagus. We report an unusual case of esophageal actinomycosis which was developed in a patient with normal immunity and improved by therapy with intravenous penicillin G followed oral amoxicillin, and we also reviewed the associated literature.

홍당무 모자이크바이라스에 관한 연구

김은수(Woon Soo Kim),최예진(Yae Chin Choi) 한국식물학회 1963 Journal of Plant Biology Vol.6 No.2

KIM, Woon Soo* & CHOI, Yae Chin**(University of Ill., U.S.A.* & Chunchon Agricultural Coll.**) Studies on the viruses of radish mosaic. Kor. Jour. Bot. VI (2):9-21, 1963. A mosaic diseased radish collected from the suburb of Seoul, in November, 1961 was used for studing the host range, physical properties, purification, insect transmission, and electron microscopy. A Japanese strain of raddish mosraic (RPV) was also used with Korean strain of radish mosaic (KRMV) for a comparative study. The two viruses, KRMA and RPV, were identified by the difference in host range, insect transmission and electron microscopy. The KRMA was severely infective on tobacco and Nicotiana glutinosa, while on Gomphrena globosa was immune to the virus. RPV produces necrotic local lesions on Comphrena globosa but did not infect tobacco and N. glutinosa. Among varieties of radish, Seoul, Akamaroo, Akanagea, Koong-Joong showed more severe symptoms than Simoo, Minong, Paek-soo, which appeared to be fainly resistant. In a number of tests, it was found that the virus KRMA retained its infectivity until to a dilution of 1:2,000, heating at 58˚C for 10 minutes, and aging in vitro for 7 days at room temperature. The RPV was not inactivated until it was diluted to 1:2,000, heated to 56。C, and aged for 6days. The KRMV was readily transmitted by the aphid (Myzus persicae Sulz). The virus RPV was not transmitted by the aphid in a number of tests. Partialy purified viruses using ammonium acetate buffer, salting-out by ammonium sulfate and centrifugation of high and low speed were highly infective. Electron micrographs showed that the KRMV paticles are of spherical particles whereas the RPV particles are rod-shaped.

하악골 절제술후 재이식된 돌결자가골 재이식 : 치험 3례

김 식,김수관,김운규,김수민,류종희,이준길,조경안,김수흥,정태영,여환호 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2

We described three cases of immediate reimplantation of a frozen-thawed autogenous mandible composed of a mixture of iliac bone, marrow, and particulate hydroxyapatite in tumors of the mandible. Acceptable outcomes were obtained in three patients who underwent immediate autogenous mandibular graft reconstruction. The conditions leading to successful outcome of the procedure are also discussed. Reimplantation of frozen autogenous lesioned mandible was performed in three patients with mandibular tumors. Two reimplanted grafts survived without complications following surgery. One case had postoperative infection that resolved with appropriate antibiotic treatment. There were no recurrences of the primary lesions. Satisfactory facial contour after surgery was achieved. These results are most promising, and we believe that, with further refinement, this technique will offer a new and acceptable modality for facial reconstruction in patients with cancer.

Microstructure‑Strengthening Interrelationship of an Ultrasonically Treated Hypereutectic Al–Si (A390) Alloy

Soo‑Bae Kim,Young‑Hee Cho,Jae‑Gil Jung,Woon‑Ha Yoon,Young‑Kook Lee,Jung‑Moo Lee 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6

Ultrasonic melt treatment (UST) was applied to an A390 hypereutectic Al–Si alloy in a temperature range of 750–800 °Cand its influence on the solidification structure and the consequent increase in strength was investigated. UST at such a hightemperature, which is about 100 °C above the liquidus temperature, had little effect on the grain refinement but enhanced thehomogeneity of the microstructure with the uniform distribution of constituent phases (e.g. primary Si, α-Al and intermetallics)significantly refined. With the microstructural homogeneity, quantitative analysis confirmed that UST was found tosuppress the formation of Cu-bearing phases, i.e., Q-Al5Cu2Mg8Si6, Al2Cuphases that form in the final stage of solidificationwhile notably increasing the average Cu contents in the matrix from 1.29 to 2.06 wt%. A tensile test exhibits an increasein the yield strength of the as-cast alloy from 185 to 208 MPa, which is mainly associated with the solute increment withinthe matrix. The important role of UST in the microstructure evolution during solidification is discussed and the mechanismcovering the microstructure-strengthening interrelationship of the ultrasonically treated A390 alloy is proposed.

Protective effect of ginsenoside Rb1 against tacrolimus-induced apoptosis in renal proximal tubular LLC-PK1 cells

Lee, Dahae,Lee, Dong-Soo,Jung, Kiwon,Hwang, Gwi Seo,Lee, Hye Lim,Yamabe, Noriko,Lee, Hae-Jeong,Eom, Dae-Woon,Kim, Ki Hyun,Kang, Ki Sung The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1

Background: The aim of the present study was to evaluate the potential protective effects of six ginsenosides (Rb1, Rb2, Rc, Rd, Rg1, and Rg3) isolated from Panax ginseng against tacrolimus (FK506)-induced apoptosis in renal proximal tubular LLC-PK1 cells. Methods: LLC-PK1 cells were treated with FK506 and ginsenosides, and cell viability was measured. Protein expressions of mitogen-activated protein kinases, caspase-3, and kidney injury molecule-1 (KIM-1) were evaluated by Western blotting analyses. The number of apoptotic cells was measured using an image-based cytometric assay. Results: Reduction in cell viability by $60{\mu}M$ FK506 was ameliorated significantly by cotreatment with ginsenosides Rg1 and Rb1. The phosphorylation of p38, extracellular signal-regulated kinases, and KIM-1, and cleavage of caspase-3, increased markedly in LLC-PK1 cells treated with FK506 and significantly decreased after cotreatment with ginsenoside Rb1. The number of apoptotic cells decreased by 6.0% after cotreatment with ginsenoside Rb1 ($10{\mu}M$ and $50{\mu}M$). Conclusion: The antiapoptotic effects of ginsenoside Rb1 on FK506-induced apoptosis were mediated by the inhibition of mitogen-activated protein kinases and caspase activation.

가동보 설치에 따른 하상변동 예측에 관한 연구

김운중,문수회,김영균,민병무 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.1

To estimate the change of riverbed geography by the operation of movable weir, two-dimensional RMA-2 and SED-2D were used in this study. Hydraulic elements like flowrate or depth were simulated by RMA-2 and the change of riverbed geography according to sediment transport was simulated by SED-2D. Suktop stream was chosen as a project river for the simulation. Under the condition of 50-year rainfall frequency, the estimated flood of Suktop was 123cms. At the points of movable weir the simulation results showed that the difference of high water level from the result of HEC-RAS was less than 0.64m. And the difference of water velocity was less than 0.21m/sec. Based on the sediment simulation by SED-2D, predicted riverbed elevation was 29.70-30.22m at the point of ST1 and 28.26~28.82m at the point of ST2. Measured riverbed elevation was 29.70-30.16m, 28.28-28.69m, respectively. So, the predicted elevations are closely similar to the measured elevation. At the point of ST1, sediment transport simulation results showed that erosion of riverbed was predicted by the operation of movable weir. This erosion was ranged up to 200m upstream and the riverbed would be eroded away as much as 0.07m for one year. At the point of ST2, upstream of movable weir would be eroded and the erosion depth was estimated 0.261m. The erosion was higher at the point of movable weir and the erosion depth was estimated 0.310m. But the sediment would be deposit downstream of the movable weir. SED-2D followed by RMA-2 is well applicable to the stream which has movable weirs and can estimate not only hydraulic parameters such as flowrate or depth but also riverbed elevation change caused by erosion or deposit of fluvial sediment.

단수수 條斑細菌病에 대한 品種間 抵抗性 差異와 羅病菌의 窒素 및 糖 含量과의 關係

金明運,白壽鳳,閔庚燦 건국대학교 1969 學術誌 Vol.10 No.1

This experiment was undertaken with the purpose of obtaining the basical data on the culturing techniques, breeding and plant protection in the cultivating of Sweet sorghum in Korea. The Sweet Sorghum 11 U.S. varieties, 2 Japanese and 2 Koreans were cultivated unders the natural condition in infecting of disease. The investigation was performed on the varietal difference in the resist trance to bacterial strip disease and the nitrogen and sugar contents in diseased leaves, and correlation between the diseased ratio and several agronomic characteristics. The results obtained are as follows. 1) It was no found that the variety exempted from disease, White collier, Missouri groytop, Rex, Rurier Var-1 and Atlas were more resisted in certain degree, Sorip-zong (Small grain var.) Tracy, Chinese, Zeuk-Jaelaizong and Honey-I were resited in medium degree and Early sumac, Sugar drip, Red amber, Ames amber and Rex orange were weaker in resistant to disease. 2) In the Correlation between the heading data and degree in resistant disease, generally, medium maturing varites were relatively strong resistant, laste varieties were weakest with exception of very few varieties. 3) In the correlation and regression between the several agronomic characteristics and diseased ratio, the curium lenth and weight of curium had no significant differences, heading date had r= -0,591*, y=90,822-0.354x and brix degree had r= -0.68**, y=19,992-0,246x. 4) The leaves of resistance varieties were more contained the nitrogen, sugar and increased C/N ratio than those of susceptibles.

韓國人 乳癌組織의 Estrogen 受容體의 檢索

金政徹,李洙定,鄭泰浩,朴朗韻,黃一愚 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

한국여성의 유방암에서 estrogen수용체 및 progesterone수용체의 분포를 알아보기 위하여 39례의 유방암조직에서 이를 측정하였다. Estrogen수용체는 33.3%에서 양성으로 5.42에서 230.02f㏖/㎎ protein까지의 값을 나타내었고 progesterone수용체는 5.34내지 66.60f㏖/㎎ protein의 함량을 가진 56.4%에서 양성이었다. 폐경기 이전은 폐경기 이후에 비해 estrogen수용체와 progesterone수용체가 모두 양성인 경우가 더 많았고 모두 음성인 경우는 더 적었으며 환자의 연령이 50세 이하인 경우의 progesterone수용체 양성율은 50세 이상의 경우보다 두배 이상 높았다. 또한 액와임파절전이가 전혀없는 경우가 1개이상 전이된 경우보다 훨씬 높은 estrogen수용체 양성율을 보였으며 TNM병기 Ⅱ는 병기Ⅱ나 Ⅳ보다 estrogen수용체 및 progesterone수용체 양성율이 더 높은 것으로 나타났다. 한편 조직학적 유형과 호르몬 수용체와의 관계는 대부분이 infiltrating ductal carcinoma인 까닭에 의미 있는 결과를 얻지 못하였다. 이상의 결과에서 나타난 estrogen수용체 및 progesterone수용체와 유방암의 여러 임상적 병리조직학적 측면들과의 상관관계로 미루어 볼 때 임상검사실에서의 통상적인 호르몬수용체 측정법 확립은 유방암의 치료 및 예후판단에 매우 유용하리라 기대된다. The levels of estrogen receptors (ER) ad Progesterone receptors (PR) in 39 breast cancer tissues have been determined and a correlation of both receptor levels with the histopathologic findings and clinical features was attempted. The ER levels ranged from 0 to 230.02 fmol/㎎ cytosol protein and the PR levels ranged from 0 to 66.60 fmol/㎎ cytosol protein. 33% of the specimens were ER+ and 56% were PR+. The group younger than 50 years had a higher percentage of positive PR status. The positive rate of ER showed no significant difference between pre and postmenopause, while those of PR was decreased from 67% in pre-to 41% in postmenopause. 82% of the histologic type were infiltrating ductal carcinoma and 74% of patients were stage Ⅱ of TNM classification. These results indicated that there was strong correlation between age, menopausal status, axillary lymph node metastasis, TNM stage and receptor status.

서울大學校學生의 赤血球沈降速度에 關한 硏究 : 結核集團檢診의 疫學的方法을 爲하야

金仁達,權彛赫,車喆煥,柳運相,尹德老,林道洙 서울대학교 1957 서울대학교 論文集 Vol.5 No.-

The Eryshrocyte Sedimentation Rate on the students of Seoul National Uuiversity was discussed corelating with X-ray findings for tuberculosis mass suruey. 1) Among the students of Seoul National University, 4727 were examined by miniature radiogram and the morbidity of active tuberculosis was shown 1.7 percent 616 of students were examined by both miniature radiogram and blood sedimentation reaction and the morbidity was 2.9 per cent. 2) The screening procedure by blood sedimentation reaction in addition to miniature radiogram could prevent missing around one fourth of active cases This means that E.S.R. has a great significance on the screening procedure for tuberculosis case findings. 3) Abnormal E.S.R. was shown on 75,5 percent of active cases. Persons whose E.S.R. are beyond 3mm for 30minutes and 10mm for 60minutes are suspected tuberculosis and should be required further examination. 4) The distribution rate of E.S.R. of between 0∼6.5mm was shown on 80percent of these students who were examined and that of between 0∼10mm was shown on 88.7 percent of students. The mean was 4.8mm. Hereby it is recommended That E.S.R. must be employed as one part of the useful methods on the mass screening survey for tuberculosis. furthermore it should be recalled that most of the Korean youths show tuberculin test positive. This fact requires such procedure as blood sedimentation reaction in addition to X-ray photography urgently on the epidemiological tuberculosis survey.