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백수봉,정일민,도은수 한국약용작물학회 1998 한국약용작물학회지 Vol.6 No.4
50종의 약용식물 추출액을 공시하여 생체외(in vitro) 및 생체내 (in vivo)에서 몇 가지 주요 종자전염성병 억제를 위한 항균활성식물을 선발하고 선발된 식물에 대한 항균활성을 검정한 결과는 다음과 같다. 가) 공시된 50종의 약용식물추출액을 공시병원균(벼도열병균, 참깨검은무늬병균, 고추탄저병균, 무·배추검은무늬병균)을 접종한 종자에 대한 항균활성을 습지법과 물한천법으로 정정한 결과 마늘, 주목, 대황, 무슬, 감초, 달맞이꽃 등에서 항균활성이 우수한 것으로 나타났다. 나) 1차 선발된 식물추출액을 종자에 희석 처리하여 감자한천법으로 검정한 결과, 마늘 추출액이 10배에서도 항균활성을 보였고 종자침적 시간에 따른 효과를 보면 긴 시간 침적이 가장 높은 항균활성을 나타냈으며 종자침적온도에 따른 항균효과를 보면 25℃ 처리가 대체적으로 우수했다. 다) 식물 추출액의 종자발아및 초기생육에 미치는 영향은 마늘 추출액의 경우 무처리에 비하여 종자발아에 차이가 없었고 초기생육은 오히려 양호하였다. 주목의 추출액은 무와 배추에서 약간의 영향이 있었고 우슬, 감초, 달맞이꽃 등에서는 종자 발아 및 초기 생육을 심하게 저해했다. Antifungal activity on major seedborne diasease of crops was screened by the treatment of the extracts from 50 medicinal plants in vitro and in vivo. The extracts of garlic and taxus, Rheum undulatum, Achiranthes japonica, Glycyrrhiza uralensis, Oenothera lamar kiana treated with the blotting filter paper and water agar methods inhibited the growth of Pyricularia oryzae, Alternaria sesamicola, Colletotrichum gloeosporioides, and Alternaria brassicicola among the tested plants. Antifungal activities on infected seeds by soaking methods were shown even at the dilution of the extracts by 10 times. The activity was the highest in soaking seeds at 25℃ for 24 hours. The effect of plant extract on seed germination was not significant as compared with untreated seed. However, early growth of seedling was increased by the treatment of extracts. The extract of taxus slightly inhibited the seed germination of radish and chinese cabbage but those of Achirunthes japonica, Glycyrrhiza uralensis and Oenthfera lamarkiana showed severe damage on the seed germination and early growth of seedling.
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白壽鳳 建國大學校附設農業資源開發硏究所 1985 農資源開發論集 Vol.10 No.-
In the cucumbers, prevention and therapy effects of fungicides, fungicide application periods, and disease development periods with Rubigan EC, Rubigan WP, Bayleton WP and Topsin-M WP as a powdery mildew control chemicals are summarized as followed; 1. In an experiment, effect of prevention, control of Rubigan EC, Rubigan WP and Bayleton WP were similar to each other. However Topsin-M WP was declined to the above fungicides. Fungicide application for inoculation of a day and five days ago was similar to each other. But inoculation of ten days ago was most decreased in control effect. The more disease development period was delayed, the more control effect was declined. 2. Effect of therapy, controlling effect of Topsin-M WP was most low. Rubigan EC, Rubigan WP and Bayleton WP were similar to each other. Treatment of inoculation period was most effective. After five days was lowest in a control effect. The more disease development period was behind the time, the more control effect was diclined.
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白壽鳳 建國大學校附設農業資源開發硏究所 1986 農資源開發論集 Vol.11 No.-
In a disease of a storage garlic, Fusarium oxysporum, Penicillium sp and Bacteria were identified. An isolated Fusarium oxysporum formed short phialides, and intercalary and terminal chlamydo-spores were present. The size of micro-conidiospores was 5~15 x 1.5~2.5㎛ and type of them was an oval, a long-oval of an egg-shaped. Macro-spores had 1~5 septs and the size of 3-septa condiosproes was 33~40 x 4~4.5 ㎛ and the type of them was a canoe-shaped. The colour of colony in a selection medium was a pale-pink and the colour of the back side in a selection medium was a pale-orange colour. An isolated Penicillium sp formed conidiospores of a basipetal chains and phialids, and the type of conidiospores was a globular-shaped. In the reasult of pathigenicity test, the pathogenicity of all of isolated pathogens was recognized and disease symptom of each of them was same. An isolated Bacteria were not identified.
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땅콩種子에서 分離된 Fusarium spp.와 그 病原性
白壽鳳,都銀洙 建國大學校附設農業資源開發硏究所 1987 農資源開發論集 Vol.12 No.-
Fusarium oxysporum, Fusarium moniliform, Fusarium solani, and Fusarium equiseti were detected from seeds of 9 peanut cultivars, and detection ratio of Fusarium oxysporum of them was the highest as 2.2% and that of the others was 0.7~1.1%. Among 9 peanut cultivars, Fusarium spp. was detected in Sae-dl, Cheon-yup-ban-lip, Sin-pung and Dae-gwang but not detected in the others, and detected in a spanish type but not detected in a semi-ruunner type, and detected in a early maturing variety and medium-late maturing variety but not detected in a late maturing variety. Their pathogenicity of Fusarium oxysporum and Fusarium solani were recognized but those of Fusarium moniliform and Fusarium equiseti were weakly pathogenic by test tube agar method. The result of pathogenicity test investigated in soil of above all pathogens to 9 peanut cultivars as follows; Fusarium solani was the most pathogenic and Fusarium moniliform was the most weakly pathogenic, and pathogenicity difference of above all pathogens according to plant type of 9 peanut cultivars was not recognized, but pathogenicity of Fusarium solani showed strongly in late variety than in early variety and medium-late variety but that of the other pathogens was not different according to maturing time of 9 peanut cultivars. And there was no resistant variety among 9 peanut cultivars.
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잿빛곰팡이病菌(Botrytis cinerea)의 藥劑耐性에 관한 硏究
白壽鳳 建國大學校附設農業資源開發硏究所 1984 農資源開發論集 Vol.9 No.-
The study has been carried out for testing of resistance of 31 strains isolated from the strawberries growing in Samrang-jin and Suwon area of Botrytis cinerea to fungicides, the characteristics of growing in media and testing of pathogenicity, and the formation of resistance strains by the method of serial transfer. 1) All strains except K-4 and K-10 showed susceptibility to Benomil, Benlate-T Sumilex and Topsin-M. 2) Botrytis cinerea isolated from Samrang-jin and Suwon area showed 6.5% of resistance to Benomil and Topsin-M. 3) Benomil resistant strain (K-4) showed the difference in the mycerial growth development control percentage on the concentration of fungicides. Benomil-susceptibilty strain (S-1) showed more than 80% of mycerial growth development control percentage in 10 ppm of all fungicides treated. 4) Captan-resistant strain(S-1) showed the cross resistance of Polyoxin, Holpet, Bramycine and not to Benomil, Benlate-T, Sumilex Benomil-resistant strain (K-4) showed the cross resistance to Captan, Polyoxin, Holpet, Bramycine, Topsin-M and not to Benlate-T, Sumilex. 5) There were difference in the characteristics of growth in media among the Benomil-, Topsin-, Captan- resistance strains and susceptible strains. 6) There were no consistant pathogenic responces between resistance-strains and susceptible strains. 7) Resistance to fungicides was obtained by the method of serial transfer and there were large differences in the resistance increase among the strains.
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白壽鳳 建國大學校附設農業資源開發硏究所 1989 農資源開發論集 Vol.14 No.-
These studies were carried out to investigate the causal organism of peanut pod rot. The results are as follows: 1) The causal organism isolated from rotted pod were Fusarium solani, F. oxysporum, Rhizoctonia solani and Bacteria. 2) These causal organism isolated from rotted pod and Pythium sp. isolated from soil of peanut field were pathogenicity on the seedling and peanut pod. 3) The incidence of peanut pod rot was increased in the injured infection, the combination infection of the causal organism and the moisture content on the peanut pod.
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