Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특징 분석(Ⅰ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.161 g/ml - 1.192 g/ml), and the other was the low density part (1.085 g/ml - 1.117 g/ml).

비혈연간 동종조혈모세포이식의 단일기관성적 : Single Center Experience

김유진,김동욱,이석,이동건,박윤희,김희제,김태규,민우성,김춘추 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2

연구배경: 비혈연간 조혈모세포이식은 HLA체계에 대한 이해의 증진과 새로운 검사법의 개발, 그리고 기증자 수의 증가에 힘입어 최근 빠르게 증가하고 있다. 방법: 가톨릭조혈모세포이식센터에서 1995년 10월부터 2001년 12월까지 비혈연간 이식을 받은 138명의 성인 환자를 대상으로 후향적 분석을 하였다. 결과: 질환별로 만성골수성백혈병 66예, 급성림프구성백혈병 23예, 급성골수성백혈병 16예, 재생불량성빈혈 21예, 골수이형성증후군 9예, 비호지킨림프종 2예, 그리고 발작성야간혈색소뇨증이 1예였다. 일차 생차부전은 분석가능한 136예 중 4예(2.9%)에서 발생하였다. Ⅱ도 이상의 급성이식편대숙주병의 발생률은 48.1%였고, 만성이식편대숙주병은 49.5%였다. 재발 이외의 사망원인 중 가장 높은 빈도를 보인 것은 폐렴으로, 독성사망 64예 중 36예(52.3%)에서 관찰 되었다. 만성골수성백혈병의 경우(n=66), 무병생존율, 재발률, 비재발성 사망률이 각각 45.7%, 11.4%, 49.2%였다. 무병생존율은 표준위험군이 고위험군에 비해 통계적으로 유의하게 높았다(50.3% vs 32.0%, p=0.03). 급성림프구성백혈병의 경우(n=23), 무병생존율, 재발률, 비재발성 사망률이 각각 30.0%와 49.4%, 44.8%였던 반면, 급성골수성백혈병에서는(n=16) 28.6%, 39.4%, 52.9%였다. 이들 급성백혈병에서는 표준위험군에 비해 고위험군에서 재발률과 비재발성 사망이 모두 높게 관찰되었다. 중증재생불량성빈혈의 경우(n=21), 무병생존율과 비재발성 사망이 37.3%와 62.7%였다. 결론: 본 연구의 결과 비혈연간 이식은 HLA적합 형제가 없는 혈액질환 환자들에게 유용한 치료법의 하나임을 알 수 있었다. 낮은 병기에서의 조기 이식과 분자생물학적 방법을 사용한 HLA 적합도의 개선이 이식관련 사망을 최소화시켜 비혈연간 이식의 효과를 더욱 확대시킬 수 있을 것으로 생각된다. Background: Unrelated allogeneic stem cell transplantation (U-SCT) is recently on a increasing trend supported by better understanding of HLA system, development of new HLA typing, and increase of donor pool. Methods: We retrospectively analyzed 138 adult patients who underwent U-SCT between October 1995 and December 2001 at Catholic Hematopoietic Stem Cell Transplantation Center. Results: Diagnoses were chronic myeloid leukemia (CML, n=66), acute lymphoblastic leukemia (ALL, n=23), acute myeloid leukemia (AML, n=16), severe aplastic anemia (SAA, n=21), myelodysplastic syndrome (MDS, n=9), non-Hodgkin lymphoma (NHL, n=2), and paroxysmal nocturnal hemoglobinuria (PNH, n=1). Primary engraftment failure was observed in 4 (2.9%) of 136 evaluable patients. Acute GVHD (≥grade II) occurred in 48.1% and chronic GVHD occurred in 49.5%. The most common cause of death other than relapse was pneumonia, which occurred 36 (52.3%) of 64 toxic deaths. In CML, disease-free survival (DFS), relapse rate, and non-relapse mortality (NRM) was 45.7%, 11.4%, and 49.2%. DFS of the standard-risk group (SR) was significantly better than that of high- risk group (HR) (50.3% vs 32.0%, P=0.03). In ALL, DFS, relapse rate, and NRM was 30.0%, 49.4%, and 44.8%, whereas corresponding figures for AML were 28.6%, 39.4%, and 52.9%. NRM and relapse rate were higher in HR compared to SR in acute leukemia. In SAA, DFS and NRM was 37.3% and 62.7%. Conclusion: We concluded that U-SCT is a feasible therapeutic option for patients lacking a HLA-matched sibling donor. Transplantation at earlier phase of disease with more accurate HLA matching by molecular typing can minimize treatment-related toxicity and could extend the benefit of U-SCT.

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅲ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.131 g/ml - 1.240 g/ml), and the other was the low density part (1.103 g/ml - 1.122 g/ml). There was not the high density part in the serum treated with PAS for removing of antibody bound HCV. Therefore, it was concluded that the HCV in high density portion were the antibody bound forms, and the HCV in the low density portion were the free forms.

C형 간염 바이러스의 세포배양(Ⅱ)

황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7)) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of Huh_(7) cells as the infectious sources. In the high density portion, the HCV genomes were not detected in the media and cells of all days except in the 2 day culture medium, but in the low density portion, the HCV genomes were detected in the media and cells of all days. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.

특발성 척추측만증에서 견인 흉요추 보조기의 효과

박원욱,남태욱,안성준,유성호,문형근 대한척추외과학회 2003 대한척추외과학회지 Vol.10 No.3

C형 간염 바이러스의 세포배양(Ⅰ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.17 No.2

In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7),) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of human hepatoma (Huh_(7)) cells as the infectious sources. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.

Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅱ)

황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2

To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.146 g/ml ∼ 1.207 g(ml), and the other was the low density part (1.089 glml ∼ 1.108 glml).

월경주기 증상의 요인 분석

이수일,유태혁,강종선,안석철,이병욱,김헌,석재호 大韓神經精神醫學會 1986 신경정신의학 Vol.25 No.1

404 nurses and nurse-aids rated their experiences of 45 symptoms(Moos' Menstrual Distress Questionnaire) of the menstrual, premenstrual and intermenstrual phase on a 4 point scale separately. The 45 symptoms were inter-related and factor analyzed separately for each phase. six common clusters of symptoms emerged from 3 phases, which were labeled concentration, motor tension, pain, pleasurable affect, autonomic reactions and water retention. Four clusters of symptoms emerged from premenstrual phase. These clusters were labeled instabillity, withdrwal reactions, depression and headache. The factor named unpleasurable affect emerged from intermenstrual and menstrual phase. Above findings were differed from the results of Moos' study in which eight basically replicated clusters of symptoms emerged from three phases. Through discussion of validity of this scale, and differences between results of Moos' and this study, we came to the conclusion that sampling of subjects, prospective longitudinal symptoms reporting, amount of variance in a symptom over the course of menstrual cycle and proper choice of factor analysis method would be salient variables to construct valid scale for measuring menstrual cycle symptoms.

편평세포암종 임파절 전이에 대한 인공 신경망 시스템의 진단능 평가

허민석,박태원,박상욱,유동수,이삼선,최순철 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: The purpose of this study was to evaluate cervical lymph node metastasis of oral squamous cell carcinoma patients by MRI film and neural network system. Materials and Methods: The oral squamous cell carcinoma patients(21 patients, 59 lymph nodes) who have visited SNU hospital and been taken by MRI, were included in this study. Neck dissection operations were done and all of the cervical lymph nodes were confirmed with biopsy. In MR images, each lymph node were evaluated by using 6 MR imaging criteria(size, roundness, heterogeneity, rim enhancement, central necrosis, grouping) respectively. Positive predictive value, negative predictive value, and accuracy of each MR imaging criteria were calculated. At neural network system, the layers of neural network system consisted of 10 input layer units, 10 hidden layer units and 1 output layer unit. 6 MR imaging criteria previously described and 4 MR imaging criteria (site I-node level II and submandibular area, site II-other node level, shape I-oval, shape II-bean) were included for input layer units. The training files were made of 39 lymph nodes(24 metastatic lymph nodes, 10 non-metastatic lymph nodes) and the testing files were made of other 20 lymph nodes(10 metastatic lymph nodes, 10 non-metastatic lymph nodes). The neural network system was trained with training files and the output level (metastatic index) of testing files were acquired. Diagnosis was decided according to 4 different standard metastatic index-68, 78, 88, 98 respectively and positive predictive values, negative predictive values and accuracy of each standard metastatic index were calculated. Results: In the diagnosis of using single MR imaging criteria, the rim enhancement criteria had highest positive predictive value (0.95) and the size criteria had highest negative predictive value(0.77). In the diagnosis of using single MR imaging criteria, the highest accurate criteria was heterogeneity (accuracy : 0.81) and the lowest one was central necrosis (accuracy : 0.59). In the diagnosis of using neural network systems, the highest accurate standard metastatic index was 78, and that time, the accuracy was 0.90. Neural network system was more accurate than any other single MR imaging criteria in evaluating cervical lymph node metastasis. Conclusion: Neural network system has been shown to be more useful than any other single MR imaging criteria. In future, Neural network system will be powerful aiding tool in evaluating cervical node metastasis.

석이로부터 분리한 페놀성 화합물의 phospholipase A_2 저해활성

김진우,송경식,유익동,장현욱,유승현,배강규,민태진 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

호알카리성 진균 Cephalosporium sp. RYM-202가 생산하는 alkaline xylanase (CX-III)의 작용에 의해 xylan 기질로부터 생성되는 주요 가수분해 산물은 xylobiose와 중합도가 4 이상인 xylooligosaccharides이었다. 이 효소는 xylobiose에 대한 분해능을 가지고 있지 않지만 xylotriose로부터는 xylobiose를, xylotetraose로부터는 xylobiose와 xylotriose를 주산물로 형성하였다. 이러한 결과들은 CX-III가 transglycosidase 활성을 소유하는 전형적인 endo-type xylanase임을 보여준다. N-bromosuccinimide에 의한 CX-III의 화학적 변화 실험 결과 효소 1분자 당 2개의 tryptophan 잔기가 활성에 관여하는 것으로 나타났다. 그러나 iodoacetamide 및 diethylpyrocarbonate에 의한 효소활성의 저해효과는 나타나지 않음으로써 이 효소의 활성부위에 cysteine과 histidine 잔기가 필수적이지 않음이 확인되었다. The hydrolysis products formed from birchwood xylan by the action of an alkaline xylanase (CX-III) from alkalophilic Cephalosporium sp. RYM-202 were xylobiose and xylooligosaccharides polymerized with more than 4 sugar molecules. This enzyme was not active on xylobiose but readily attacked xylotriose accumulating xylobiose as a major product. The predominant end-products from xylotetraose by CX-III were xylobiose and xylotriose. These results indicate that the enzyme is typically endo-type xylanase possessing transglycosidase activity. Chemaical modification of CX-III with N-bromosuccinimide revealed that two tryptophan residues per molecule of CX-III were essential for its catalytic activity on xylan. On the other hand, iodoacetamide and diethylpyrocarbonate did not influence the activity of the enzyme, suggesting that cysteine and histidine residues are not involved in the active site of this alkaline xylanase.