Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

한국의 물가안정목표제 운용경험과 정책과제

전성인 弘益大學校 經濟硏究所 2005 經濟硏究 Vol.20 No.-

싱글 튜브 시스템 BTA 工具를 使用한 SM55C 鋼의 深孔 加工 特性에 관한 硏究

全泰玉,全彦燦,沈成輔,張性圭 동아대학교 공과대학 부설 한국자원개발연구소 1994 硏究報告 Vol.18 No.2

The BTA deep hole machining has an increasing demand because of its wide applications and its good productivity. The main feature of the BTA drill is that the tool cutting edges are unsymmetrically located on the boring head. This provides a stabilizing cutting force resultant necessary for self guidance of the boring head. The BTA drills are capable of machining for having large length to diameter ratio in single pass. The study of the accuracy and surface finish of holes produced would reveal quite useful information regarding the process. This thesis deals with the experimental results obtained during single tube system BTA machining on SM55C steel for different machining conditions.

SGML을 이용한 제안서, 보고서 관리시스템 기술 개발

전병현,한치근,이상훈 경희대학교 산학협력기술연구원 1997 산학협력기술연구논문집 Vol.3 No.-

We designed an SGML-Based Proposal and Report Management System in this paper. In this system, SGML files are stored in a database and can be searched using keywords. Hence, the documents can be handled efficiently. The relational database structure is recommended for the system and also a hybrid approach is discussed for multimedia information in the text. Functions for each module of the system are defined and conceptual and practical design issues are provided.

Effect of Mn and C on Age Hardening of Fe–Mn–Al–C Lightweight Steels

Sung‑Won Park,Jun Young Park,Kyong Mox Cho,Jae Hoon Jang,Seong‑Jun Park,Joonoh Moon,Tae‑Ho Lee,Jong‑Ho Shin 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3

The effects of Mn and C content on the age hardening of Fe–Mn–Al–C lightweight steels, which have austenitic or duplex(austenite and ferrite) microstructures, were investigated. An increase in Mn content induced a delay of the age hardeningthat is caused by the formation of intra-granular κ-carbides. In order to interpret the effect of Mn content, first-principlescalculations were conducted using the supercells of Fe24Al8C8,Fe24Al8C7,Fe24(Al7Mn)C8, and Fe24(Al7Mn)C7. The calculationsshowed that an increase in Mn content could be the source of the delay of the intra-granular κ-carbide formation bysuppressing C atom’ occupation of the vacancy at the body-centered site of L12. An increase in C content accelerated theformation of intra-granular κ-carbides, which induced the intense age hardening, and coarse inter-granular κ-carbides, whichresulted in significant decrease in impact absorbed energy due to inter-granular fracture.

국내 생산 재생골재의 품질수준 평가

전명훈,이세현,이성복,이도헌 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Recycling waste concrete is very important for environmental pollution prevention, overcome aggregates shortage and effective use of resource. The examples of using recycled aggregates such as ground backfill, road construction, concrete production. But, aggregate for structural concrete requires high degree of quality. We investigated the quality of domestic recycled aggregates in order to activate the use of recycled aggregates to seek the technologically, politically supplement factors to contribute to the effective use of recycled aggregates which has ever been a national problem, and which would solve the aggregates shortage.

지적노동자의 생산성 측정에 관한 연구

전상봉 대구산업정보대학 2000 논문집 Vol.14 No.1

This research has the productivity measurement index, by conferring on existent documents, into "efficiency," "productivity," and "changing percent of organization" and also a measurement model by the theoretical approach. Through the materials of the model company, this research has measured the knowledge worker productivity between companies from its gap by their comparison year against their basis year and has proved which index had resulted in the UP-DOWN of the productivity. As a result, this research could divide the companies within the group according to the superiority of the knowledge worker productivity and also could find out the reason of the superiority through the associated research with related personnel within the group. In addition, the superior companies could make the higher morale by giving several incentives and they could help others to share the business informations within the group. We know that the success of a company is due th a leader of it. But in the past, companies estimated the work of them-leaders and personnel according to their year goods sale in the negligence of several different conditions and variables under the business circumstance. This research, however, has concluded that giving the returns in proportion to the personnel result is desired with relation to the healthy business condition and the classified productivity of a company. In conclusion, it is believed that a competent company to with the information-intensive age can be made only under the scientific operation and management of a company.

비구개관낭종에 관한 X선학적 연구

전찬덕,이상래,황의환 大韓口腔顎顔面 放射線學會 1993 Imaging Science in Dentistry Vol.23 No.1

The purpose of this study was to evaluate the clinical and radiographic features of 35 cases of nasopalatine duct cyst by means of the analysis of periapical and/or occlusal radiograms in 35 persons visited the Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyung Hee University and Chonbuk National University. The obtained results were as follows: 1. The incidence of nasopalatine duct cysts was higher in males(74.3%) than in females(25.7%). 2. The nasopalatine duct cysts were the most frequently occurred in the 4th decades(34.3%). The 6th and 7th decades(17.1%, 17.1%) were next in order to frequency followed by the 5th decades(14.3%), the 3rd decades(8.6%), the 2nd decades(5.7%), and 8th decades(2.9%). 3. In the signs and symptoms of nasoplatine duct cysts, 25.7% were swelling, 17.1% were swelling and tenderness, 20.0% were swelling and pain, and 37.2% were a symptom. 4. In the shape of nasopalatine duct cysts, 40.0% were round type, 48.6% were oval type, and 11.8% were heart type, In symmetry of the nasopalatine duct cysts to the median palatine surture, 85.7% were symmetry, 14.3% were asymmetry. 5. In the width of nasopalatine duct cysts, 11.4% were 6-10mm, 48.6% were 11-20mm, 25.7% were 21-30mm, and 14.3% were 31-40mm. 6. In the periphery of nasopalatine duct cysts., 82.9% were distinct, 17.1% were relatively distinct. 7. In the change of root, 51.5% were intact, 17.1% were root divergence, 20.0% were root resorption, and 11.4% were root divergence and resorption.

체내 Glycogen 함량과 운동과의 관계에 관한 이론적 연구

金鍾貴,金承烈 忠南大學校體育科學硏究所 1985 體育科學硏究誌 Vol.3 No.1

This review was based on the premise that it is “what comes out”rather than “what goes in,” which provides the clues to carbohydrate, specifically muscle glycogen for athletic performance. Through the search on the related literatures, the authors reached the following conclusions. 1. Glycogen is a kind of polysaccharide formed, stored, and breaked down in the animal liver and muscles. 2. Muscle glycogen, specifically is an obligatory fuel for the aerobic and anaerobic metabolism. 3. Muscle glycogen content is a limiting factor to athletic performance required endurance. 4. When muscle glycogen level is elevated by exercise and dietary regimens, athletic performance will be improved.