Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

全南 南部와 西部海域의 海洋物理, 化學的 特性

田承寬,金相根,朴榮齋,李貞愛 木浦大學校沿岸生物硏究所 1984 沿岸生物硏究 Vol.1 No.1

Seasonal variations of physico-chemical factors were studied in both southern and western coastal waters of Chon Nam Province during a decade from 1971 to 1980. The variation of water temperature in the investigated areas was within the range of 3.6-27℃ throughout the research period. The temperature of western coastal water was lower than that of southern coastal water by 2∼4℃ in February. In spring and summer, water temperature of Jindo-Jodo area was characteristically lower than any other area by 1.0∼1.5℃. The aslinity varied within the range of 25.97∼34.32%0 throughout the research period, and showed its maximum value in February and its minimum in September. Western coastal water was always less saline than southern coastal water. Dissolved oxygen varied widely within the range of 4.25∼58.75㎖/ℓ,and showed the lowest value in September and the highest in February. Concentrations of nitrite, Phosphate, and silicate varied within the range of 0.01∼2.96 ㎍-at/ℓ, 0.06∼2.07㎍-at/ℓ, and 2.13∼29.-01 ㎍-at/ℓ, respectively. Nitrite and silicate reached their maximum concentrations in summer but phosphate reached its maximum concentration in winter. These nutrients appeared abundantly in Sinan-Mokpo area and Waned-Kangjin area, under influence of the Yongsan liver and the Tamijin liver, respectively. Transparency of waters in the investigated areas varied within the range of 0.1∼5.0m with the tendency of being lower in southern coastal area than in southern coastal area than in western coastal area. Maximum value of transparency appeared in summer, and minimum value in winter.

소양호 퇴적물에 함유된 인의 존재형태와 용출가능성에 대하여

전상호,박용안 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

Fractional composition and mobility of sediment phosphorus in Lake Soyang are investigated. The fractional scheme was made for four chemically defined hposphorus forms as adsorbed phosphorus, non apatite inorganic phosphorus(NAI-P), apatite phosphorus and residual phosphorus. These forms were measured by chemical extraction techmique. The most abundant fraction is residual phosphorus and the secondly abundant form NAI-P, and thirdly apatite phosphorus, and finally adsorbed phosphorus. The most efficient processes of scavening of dissolved phosphours in the lake are biological uptake and chemical complexation. To evaluate the eggects of pH on the mobility of sediment phosphorus, incubation experiments under aerobic and different pHcondition of leaching solution are accomplished. The results show the pH of the leaching solution has strong influence on the release of sediment phosphorus and increases of leaching in high pH aremore apparent in the NAI-P rich sediments than those in the residual P rich sediments.

貨幣의 內生性에 관한 實證的 硏究

金聖寅 홍익대학교 경제연구소 1993 經濟硏究 Vol.9 No.-

Au를 공침한 SnO₂후막센서의 가스 감응 특성

성정훈,강대원,이윤수,임준우,홍영호,이덕동 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

High selective and sensitive thick film type methane gas sensors were fabricated and their sensing characteristics for various hydrocarbon gases were investigated. Raw material, SnO_(2)/Au, prepared by coprecipitation was even grain size. SEM, TEM, BET and XRD analyses were carried out for investigation of surface morphology and crystalline structure. The thick film devices using the above materials exhibited high sensitivity to methane gas at the operating temperature of 400°C. The sensitivity of SnO_(2)/Au+Pd thick film devices to methane gas was higher than that to the other hydrocarbon gases such as iso-butane and propane. The optimal adding amounts of Au and Pd were 1wt.% and 3wt.%, respectively. The sensitivity, S, is defined as {(R_(A)-R_(G))/R_(A)}><100, which R_(A) and R_(G) are resistance in air and that in the gas ambient, respectively.

貨幣의 中立性에 관한 실증적 고찰 : 미국의 경우 A Case of the U.S.A

全聖寅 홍익대학교 경제연구소 1991 經濟硏究 Vol.7 No.-

허혈성 심질환에서 나타나는 항산화지수, 사이토카인 및 염증지표의 변화

전상훔,안지훈,방덕원,온영근,현민수,김성구,권영주 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Purpose : As diet habits change and the elderly popuation increases conronary artery disease(CAD) has also increased. Recent ongoing studies on varous inflammatory indexes in acute coronary syndrome(ACS) are underway. Several study results have shown that total antioxidant status(TAS) was remarkably lower in unstable angina than in chronic stable angina and levels of interleukin-(IL-6), C-reactive protein(CRP), monocyte chemoattractant protein-1(MCP-1), P-selectin were reported to be elevated in unstable angina and acute myocardial infarction(AMI). In our study, we studied and compared the level changes of cytokines and inflammatory indexes that play important roles inside the blood vessel. Methods : Study subjects were patients who were admitted and underwent coronary angiography at the cardiology department in Soonchunhyang university hospital. Subjects were divided into 4 groups, a control group, a stable angina and unstable angina group and an acute myocardial infarction group. The clinical findings, TAS, inflammatory cytokines(P-selectin, MCP-1, IL-6), anti-inflammatory cytokines(IL-10), Homocysteine and CRP levels in each group were measured and compared. Results : The total number of study participants was 86 patients, with 26 patients in the control group, 21 patients with stable angina, 15 patients with unstable angina, and 24 patients with AMI. There were significant differences in the TAS among the 4 grouops. 1.7±0.51 mmol/L, 1.6±0.60 mmol/L, 1.4±0.20 mmol/L, 1.4±0.29 mmol/L respectively. IL-6 levels also showed a significant difference between groups, averaging 0.8±2.47 pg/ml, 2.7±3.77 pg/ml, 6.8±10.63 pg/ml, 10.9±17.43 pg/ml respectively. Homocysteine levels averaged 8.0±2.47 pg/ml, 6.8±10.63 pg/ml, 10.9±17.43 pg/ml restectively. Homocysteine levels averaged 8.0±1.0 μmol/L in the control group, 14.3±5.14 μmol/L in patients with stable angina, 16.0±4.66 μmol/L in patients with unstable angina, and 17.3±10.08 μmol/L in AMI patients. Conclusion : Compared to the control group, the TAS levels were significantly lower in patients with unstable angina and AMI. there was no difference between the control group and those with stable angina. IL-6 and homocysteine levels were also higher in patients with unstable angina and AMI. Therefore we concluded that measurements of TAS, IL-6 and homocysteine are useful in measuring the inflammatory index of coronary artery diseases and may be important factors in predicting the states and prognosis of cardiovascular disorders.

경포호의 준설에 의한 수질개선 가능성에 관하여

전상호,김휘중 江原大學校 附設 環境硏究所 1990 環境硏究 Vol.7 No.-

Fractional composition of surface and core sediment samples were analysed to collect quality improvement and weed control in eutrofied brackish Lake Kyungpo. Factional scheme for sediment phosphorus was made for four chemically defined phosphorus froms as adsorbed phosphorus, nonapatite inorganic phosphorus, apatite phosphorus and residual phosphorus. The analysis of surface sediment samples shows that Lake Kyungpo sediments have high labile hosphorus fraction and thus have high water phllution potential. It was found that no significant water quality improvement could be expected by dredging dowm to 1.0m depth of sediments.

공정 조절에서 이상원인의 제거 방법에 대하여

전상표 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Assignable causes producing temporary deviation from the underlying system can influence on process adjustment and process monitoring in dynamic feedback control system. In this paper, Chapter Ⅱ will summarize the methods of readjusting unadjusted disturbance when assignable causes occur in dynamic process adjustment, and of removing the causes of assignable causes.