토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

흰쥐 척수 손상후 Parvalbumin과 Calbindin D-28K 함유신경세포에 관한 면역세포화학적 연구

김종중,정윤영,임유택,박상수,박영란,김현곤,문정석 조선대학교 부설 의학연구소 2002 The Medical Journal of Chosun University Vol.27 No.2

Background and Objectives: This study was done to compared the distribution of the two calcium-binding proteins immunoreactive neurons, parvalbumin immunoreactiv (PV-IR) and calbindin D-28K immunoreactive (CB-IR) neurons in the spinal cord after transection. Materials and Methods: Twenty rats were divided into 3 groups (control, complete spinal cord injury (SCI), and right and left spinal cord hemisection). SCI was produced by cutting the spinal cord use blades 11 with scalpel handles. Results: In this experiment, CB-IR neurons were mainly found in many pyramidal cells distributed in the brain stem and spinal cord of rats. PV-IR Neurons were demonstrated in all lamina of the gray matter of the spinal cord. These immunoreactive cells had the highest density in the layer I and II of dorsal horn and several nuclei of the ventral horn of the all the segments of the spinal cord. CB-IR neuropil labeling was strongly noted in all the segments of the spinal cord. In contrast PV-IR neurons were different in distribution, size and morphology in the spinal cord. The number of PV-IR neurons were greater than in the spinal cord compared with the CB-IR neurons. CB-IR and PV-IR somata were round, oval, spindle and polygonal in shape, and were unipolar, bipolar, multipolar and horizontal in types. The diameters of the somata of the PV-IR and CB-IR neurons were 40-50 ㎛, respectively. Also dendrites of PV-IR and CB-IR neurons were densely arrayed in network.

복합 관동맥병변에서의 Rotational Atherectomy : 초기결과

김진우,박성욱,홍명기,이상곤,이일수,이철환,최기준,강덕현,송재관,김재중,박승정 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

한국산 족부 백선균의 항진균제 감수성에 관한 연구

김승곤,김태전,이태수 서울保健大學 1997 論文集 Vol.17 No.1

Minimum inhibitory concentration(MIC) of griseofulvin, imidazole derivatives such as ketoconazole, econazole, miconazole and itraconazole on 52 strains of Trichophyton ruhrum, 22 strains of T. mentagraphytes, 10 strains of Microsporum spp. and 1 strain of Epidermophyton floccosum were determined after one week of incubation at 25C on Sabouraud dextrose broth. The results were summarized as followes : 1. The MICs of griseofulvin against 4 different tinea pedis fungi were ranged from 10-100mcg/ml. This values indicated that effectiveness of antifungal activity were relatively lower than those of three other imidazole derivatives such as ketoconazole, econazole, miconazole and one itraconazole. 2. Out of three imidazole derivatives, econazole had relatively higher inhibitory concentrations with 0.1-10mcg/ml against all tinea pedis isolated. 3. Out of 5 antifungal agents, itraconazole which is trizole derivatives showed the highest inhibitory activity with 0.1-10mcg/ml against all tinea pedis tested. 4. These results indicated that inhibitory activity of griseofulvin against tinea pedis were lower than those of four other antifungal agents tested. But these four antifungal agents such as ketoconazole, econazole, miconazole and. itraconazole were effective against tinea pedis tested.

사회유형과 행동가치에 관한연구

김상철,김한곤 용인대학교 학생생활연구소 1993 학생생활연구 Vol.1 No.-

The transformation and change of society since the turn of the century has been breathtaking. we have progressed from a basically agrarian society to a dynamic, industrial, post-industrial, society with a higher level of standard of living than was ever thought possible. as to the change there is no doubt. we continue to live in the society that have rapidly chanted. we have begun to recognize that social change inalves not only changes in social patterns but also in behavioral value. therefore we emphqsized the importance of hehavioral value in social pattern in order to social goal. we must first of all be considering social pattern and behavioral value. although the emphasis in this study will be on human behavior development, we must recognize the patterns in socety in which most people live are socisl gatterns comprised of many interrelated subpatterns only one of which is a human behavior as to agarian, industrial and post-industrial society. the focus of the agarian society is on anthority and custon modern society is on value and belief and contemporary society is on consumption and other-directed. the havioral criterion of agarian society tradiional society in this study emphasized the ascriptive, paarticularistic, diffure patterns and deferential s-tratification system. Industrial society (modern society) emphasized the save in scarcity psycholong. post-industrial society (contemporary society) emphasized the con-sumption in abundance psychology.

비지가 발생하지 않는 두유 제조공정 및 전두유의 특성

김성란,박용곤,석호문,오승훈 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1

Whole soybean milk is a functional soymilk produced by mixing of soymilk and Bejee (soymilk residue) slurry. Application of enzymatic technology for disintegrating the coarse and fibrous materials in soymilk residue, and development of biomill reactor for reducing reaciton time make possible to produce new soymilk in domestic industry. Whole soybean milk is environmental affiliated product without discharging any waste such as Bejee ets. Whole soybeen milk has good taste and flavour without coarse mouthfeel induced by including Bejee particle, as well as contains more dietary fiber, protein, oligosaccharide, and other functional compounds than general soymilks. Important values of whole soybean are utilizing all nutritional properites from whole soybean without dicarding any of the insoluble solid. In this study, producing technologies and nutritional properties of whold soybean milk were investigated.

食道 및 氣管異物에 對한 統計的 考察(第4報)

金善坤,趙商昊,龐會珍,林章善,李喜鎔 대한이비인후과학회 1969 대한이비인후과학회지 두경부외과학 Vol.12 No.3

국가 대표급과 제주도 고교 대표급 수영선수들의 그랩 스타트의 운동학적 분석

김철원,김승곤,오만원,임상용,김성찬,이창준,남사웅,이세형,류재청,신석종,김세민 濟州大學敎 體育科學硏究所 1998 체육과학연구 Vol.4 No.-

The purpose of the study was to undertaken to analysis the Grab starting technique between national representive team(3) and Cheju's representive team(3) with 3D cinematography. The conclusions obtained were as follows: Cheju's showed more elapsed time than national representive team on starting block. and more vertical than horizontal direction of COG position, more concentrated vertical velocity & acceleration than forward direction. Therefore It is necessory for cheju's to train balanced trust force in horizontal and vertical direction in grab starting technique.

Penicillinase Producing N.gonorrhoeae株와 Nom-Penicillinase Producing N.gonorrhoeae株의 약제 감수성에 관한 비교연구

김승곤,김호훈,김태전 서울保健大學 1990 論文集 Vol.10 No.1

Studies were carried out to evaluate the actual status of the carriers infected with N. gonorrhoeae among the high-risk populations over the country and to determine the drug resistance patterns of β-lactamase producing N. gonorrhocae isolates, in particular attention to the recent increasing discoveries of Penicillinase producing Neisseria gonorrhoeae strains this country. The results could be summerized as follows: 1. The isolation rate of N. gonorrhoeae from the suspicious gonorrhoeal patients (men) were 37.5% on the average, out of a total of 817 examined, but from the prostitutes or bar-girls the isolation rate showed 9.4% on the average out of 3.609 examined, of the isolated 644 gonorrhoeal strains, one hundred and fifty-three strains were detected as β-lactamase producing N. gonorrhoeae. The average detection rate of β-lactamase producing N. gonorrhoeae was 23.8%, but the frequency of PPNG detection was much different depening on the areas where the specimens collected ranging from 3.8%(Chun buk do) to 41.2%(Kyung buk do), except the area Jaeju island where on PPNG was detected. 2. Most non-PPNG strains were able to be inhibited at the concentration of the drugs below 32ug/ml of Penicillin G and 8ug/ml of Ampicillin, but nearly half or more than half of the β-lactamase producing N. gonorrhoeae strains were not inhibited at 32ug/ml of the drugs, In other words, β-lactamase producing N. gonorrhoeae had acquired high resistance to Pehicillin G and Ampicillin. 3. In susceptibility of PPNG and non-PPNG to spectinomycin, Kanamycin, Tetracycline, Erythromycin, Cefuroxin, Cefoperazone, it seemed to be no significant diffevence, but some exceptions showing a peculiar drug resistance to 16ug/ml of Cefuroxin, 64ug/ml of Kanamycin, 512ug/ml of Spectinomycin or 32ug/ml of Tetracycline. And also a few multi-resistance like PPNG strains could rarely be detected showing resistance to the concentration of Tetracycline 32ug/ml, Cefuroxin 16ug/ml, Kanamycin 64ug/ml. 4. In the correlation of inhibitory Zone size dise diffusion method on the MICS by agar plate dilution method to 89 N. gonorrhoeae strains with Pencillin G, Ampicillin, Erythromycin, Tetracycline, and chlorampenicol, Ampicillin showed the highest correlation coefficient as an absolute value showing r=-0.7598, which had negative correlation. Through these studies β-lactamase producing N. gonorrhoeae strains were able to be found from the specimens collected through all over the provinces of the country except the Island of Jaejudo hopsitols.

血液 및 尿中 水銀濃度의 統計學的인 比較 檢討

김승곤,김태전 서울保健大學 1986 論文集 Vol.6 No.1