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심순미,김건희 德成女子大學校 1998 德成女大論文集 Vol.29 No.-
Anthocyanin pigment is extensively extensively distributed in fruit juices shich are popular to consumer owing to valuable components such as vitamin, mineral, fiber, enzymes etc. Anthocyanin is using for detecting adulteration adn quality factor of fruit juices. So, research on the stability of anthocyanin is actively progressing not only using natural colorants also improving quality of fruit juices. And anthocyanin have only recently begun to be regarded as biologically active substances as well as colrants. Morever, fruits such as pear, apple, grape, plum which are widely produced in Korea containing more anthocyanins than any other pigments. However major problem associated with the storage of anthocyanin is their unstability, caused by certain enzymes, pH, temperature, metal complex, copigments.
한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고
최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1
The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.
Shim, Soon-Mi The Korean Society for Applied Biological Chemisty 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.2
Effect of leek (Chinese chive) on bioaccessibility of arsenic (As), cadmium (Cd), and lead (Pb) was determined in comparison with sodium copper chlorophyllin (SCC) using in vitro digestion model. Leek (0, 6, 12, 60, and 120 mg) and SCC (0, 1, 5, 10, and 50 mg) were digested with equal concentration (10 ppm) of As, Cd, and Pb. Concentration of each heavy metal in aqueous phase following in vitro digestion was measured using an inductively coupled plasma optical emission spectrometer. Changes in absorbance spectra of chlorophyll extracted from leek and SCC by heavy metals were measured at 0, 1, 2, and 3 h after mixing. Results showed that the concentration of each heavy metal in aqueous phase decreased with increasing amounts of leek and SCC. At the highest level of leek (120 mg), the concentrations of As, Cd, and Pb decreased to 93.9, 87.1, and 58.2%, respectively. Absorbance of chlorophyll drastically decreased after 1 h of mixing with each As, Cd, and Pb, and no difference in the absorbance was observed after 2 and 3 h. This result indicates that mixing chlorophyll extracted from leek with each heavy metal for 1 h was sufficient for chelating As, Cd, and Pb ions. On the other hand, SCC and each heavy metal showed a decreasing pattern of absorbance without any significant difference for 3 h, indicating that chlorophyll from leek was more effective than SCC, a commercial grade chlorophyll derivative, in chelating As, Cd, and Pb. Results showed leek reduces heavy metals in humans.
Shim, Soon-Mi,Yi, Ha-Lim,Kim, Young-Suk Informa Healthcare 2011 International journal of food sciences and nutriti Vol.62 No.8
<P>Antioxidant activity, total phenolic content, and flavonoids in onion were determined using the DPPH method, the Folin-Ciocalteu method, and high-performance liquid chromatography analysis, respectively. Myricetin was the most bioaccessible among flavonoids in both red and yellow onions after digestion. The result suggest that the antioxidant activity of yellow and red onions was stronger in the outer layer than in the inner layer and strong correlation was found between antioxidant activity and total phenolic content (<I>r</I><SUP>2</SUP> == 0.927).</P>
Shim, Soon-Mi,Kim, Gun-Hee The Korean Society of Food Science and Nutrition 2001 Preventive Nutrition and Food Science Vol.6 No.1
Pigment extracts obtained from Star Ruby grapefruit pulp were stored at different temperatures (4.5$^{\circ}C$, 23$^{\circ}C$) and exposed to light. many carotenoid oxidation products were formed due to light-exposure during storage periods. They were monitored by using HPLC with photodiode array detection and tentatively identified. Including (all-E)-lycopene and trans-$\beta$-carotene as predominant carotenoids in red grapefruit, 5Z-lycopene, 9Z-lycopene, 13Z-lycopene, and 15-cis-$\beta$-carotene were formed at 4.5$^{\circ}C$, 23$^{\circ}C$. Degradation of all-tarns lycopene was more susceptible to light-exposure and temperature a than that of all-trans $\beta$-carotene. The formation of lycopene cis isomers was favored under lighted condition. Respectively, (5Z)-lycopene was formed in greater amounts than other isomers at 23$^{\circ}C$ storage. The concentration of 15-cis-$\beta$-carotene was significantly increased during storage at 23$^{\circ}C$ storage. The concentration of 15-cis-$\beta$-carotene was significantly increased during storage at 23$^{\circ}C$.
꽃향유(Elsholtzia splendens) 추출물의 안전성 평가 : 급성독성과 돌연변이성 평가
Shim, Soon-Mi,Choi, Mi-Hee,Kim, Gun-He 덕성여자대학교 대학원 2007 덕성여자대학교 대학원 논문집 Vol.9 No.-
Much attention is recently gained for Elsholtzia splendens extracts and issue on their usage is raised due to their healthful properties. However, there is no sufficient background information on toxicological evaluation of Elsholtzia splendens extracts to give an assurance of safety for developing dietary supplements and functional foods. The objective of this study was to evaluate safety on Elsholtzia splendens extracts using acute oral toxicity, bacterial reverse mutation, and chromosome aberration test. Total flavonoids within Elsholtzia splendens were extracted with 80% of methanol by a reflux condenser. Both female and male mice were orally administrated Elsholtzia splendens extracts at the doseof 0, 500, 1000, and 2000 mg/kg body weight/day. Mutagenicity of the extracts was evaluated in a bacterial reverse mutation assay using histidine-requiring Salmonella typhimurium (TA 98, TA 100, TA 1535, and TA 1537) and tryptophan-requiring Escherichia coli (WP2uvrA). In vitrochromosome aberration assay in Chinese Hamster Lung (CHL) was conducted to evaluate genotoxicity. Single administration of dose levels of 500, 1000, and 2000 mg/kg body weight/day to mice for 15 days did not produce any significant mortality, clinical signs, body weight loss, and gross findings. Elsholtzia splendens extracts in the range of 156.3-5000 μg/plate did not induce mutagenicity in S. typhimurium and E. coli with and without metabolic activation system. Any significant chromosomal aberration was not observed in CHL cells 6 hrs after treating with the extract at the concentrations of 1250, 2500, and 5000 μg/mL in absence and presence of metabolic activation system. However, frequency of chromosomal aberration in 22 hrs after treatment without metabolic activation system was increased with showing a pattern of dose-response relationship. The highest concentration of 5000 μg/mL significantly induced chromosomal aberration. Elsholtzia splendens extracts may induce chromosomal structure abnormality in CHL cells. This study suggests that further study is needed to assess the potential genotoxic effects of Elsholtzia splendens extracts.
Soon-Mi Shim,Gun-Hee Kim 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.1
Pigment extracts obtained from Star Ruby grapefruit pulp were stored at different temperatures (4.5℃, 23℃) and exposed to light. Many carotenoid oxidation products were formed due to light-exposure during storage periods. They were monitored by using HPLC with photodiode array detection and tentatively identified. Including (all-E)-Iycopene and trans-β-carotene as predominant carotenoids in red grapefruit, 5Z-Iycopene, 9Z-lycopene, 13Z-lycopene, and 15-cis-β-carotene were formed at 4.5℃, 23℃. Degradation of all-trans Iycopene was more susceptible to light-exposure and temperature than that of all-trans β-carotene. The formation of Iycopene cis isomers was favored under lighted condition. Respectively, (5Z)-Iycopene was formed in greater amounts than other isomers at 23℃ storage. The concentration of 15-cis-β-carotene was significantly increased during storage at 23℃.