연탄재를 이용한 중금속 폐수의 처리

박수영,조광명,정재기 ( Soo Young Park,Kwang Myeung Cho,Jae Kee Jeong ) 한국폐기물자원순환학회(구 한국폐기물학회) 1992 한국폐기물자원순환학회지 Vol.9 No.2

Kluyverromyces fragilis의 Alkaline Phosphatase 유전자의 구조 분석

박수영,황선갑,하상철,김종국,박완,홍순덕,Park, Soo-Young,Hwang, Seon-Gap,Ha, Sang-Chul,Kim, Jong-Guk,Park, Wan,Hong, Soon-Duck 한국미생물·생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.1

From the pSKH201 plasmid which had been previously cloned in our laboratory, a 3.0kbp insert DNA encoding the alkaline phosphatase of Kluyveromyces fragilis was cleaved with several restriction endonucleases and ligated int the appropriate sites of M13mp18/19 vectors and sequenced by Sanger's dideoxy chain termination method. The sequence contained a 1,638 bp open reading frame(ORP) whose similarities in nucleotide, when compared with those of Saccharomyces cerevisiae and Escherichia coli by GENETYX program, were found to be 61% and 46%, respectively. The deduced amino acid sequence consists of 546 amino acids and contains several homologous regions in the alkaline phosphatases of E. coli, S.cerevisiae and human placenta.

당뇨유발쥐에서 닭의장풀의 혈당감소효과와 간조직내의 Glucose-6-Phosphate Dehydrogenase의 효소활성에 미치는 효과

박수영,조경혜,Park, Soo-Young,Cho, Kyung-Hea 한국생약학회 1994 생약학회지 Vol.25 No.3

The hypoglycemic and metabolic effects of Commelina communis L. extract were investigated in alloxan induced diabetic rats. The increased blood glucose level in the diabetic rats was significantly reduced and the loss of body weight was recovered with the treatment of the plant protein fractions($30{\sim}70%$ ammonium sulfate precipitates). Administration of the plant protein fractions elicited the significant increase of glucose-6-phosphate dehydrogenase (G-6-P DH) activity and liver weight which were decreased in the diabetic rat liver. G-6-P DH was partially purified from extract- or insulin-treated diabetics, diabetic control, and normal rat liver and studied for the biochemical properties. The $K_m$ value(9.002 mM) of diabetic rat liver enzyme was greatly higher than that (0.033 mM) of normal enzyme indicating the affinity of enzyme for the substrate was significantly reduced in the diabetic rat liver. This reduced affinity of enzyme for the substrate in the diabetic rat was recovered in the extract- or insulin-treated rat liver enzyme having 0.164 or 0.208 mM of their $K_m$ values, respectively. Although there was no significant difference in the optimum pH(6.0) and optimum temperature($37^{\circ}C$) of enzyme among the experimental groups, the dependence of their activities on pH appeared to be slightly resistant in the extract- or insulin-treated group compared to the diabetic group. In order to investigate the antigenicity of rat liver enzyme among experimental groups, enzyme-linked immunosorbent assay was carried out by using anti-G-6-P DH anti-serum. Absorbance(0.102) shown in the normal rat liver was reduced even below zero in the alloxan-diabetic rat liver, but increased again in the extract- or insulin-treated rat liver(0.096 or 0.118, respectively). The result of this study suggested that G-6-P DH may be used as a marker enzyme to diagnose and to indicate the progress of the diabetics, and the hypoglycemic effect of the extracts of Commelina communis L. was certainly associated with action or mode of G-6-P DH on the rat liver.

도시행정제도의 비교국가적 연구 - 영국 , 프랑스 , 독일을 중심으로 -

박수영,이효 ( Soo Young Park,Hyo Lee ) 한국지방행정연구원 1995 地方行政硏究 Vol.10 No.1

고구마 배양세포에서 Peroxiredoxin cDNA의 분리 및 발현 특성

박수영,류선화,권석윤,김종국,곽상수,Park, Soo-Young,Ryu, Sun-Hwa,Kwon, Suk-Yoon,Kim, Jong-Guk,Kwak, Sang-Soo 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2

Peroxiredoxin(Pix) are large family of peroxidases that reduce alkyl hydroperoxides and hydrogen peroxide. A cDNA clone (referred to as swPrxl) encoding Pix was from a sweetpotato cDNA library constructed from suspension-sultured cells, and its expression was investigated in terms of stress. The swPrxl contained an open reading frame (ORF) encoding mature protein of 193 amino acids with calculated molecular mass of 20.8kDa. The predicted amino acid sequence of swPrxl has two conserved cysteines that are essential resicues for the reduction of peroxides. It showed high amino acid sequence homology ot PixIIF of Arabidopsis (77%) and putative Prx of rice(72%). RNA gel-blot analysis showed that swPrxl gene was expressed dominantly in leave among intact tissues, and also highly detect in suspension-cultured cells. Interestingly, the level of swPrxl transcripts was almost the same regardless of the growth stage in suspension culture. Furthermore, the transcription level of swPrxl gene was not significantly changed in response to various stress treatments such as wounding, extreme temperature and stress-related chemicals RT-PCR analyses.

Espression of Alkaline Phosphatase Gene from Kluyveromyces fragilis in E. coli and S. cerevisiae

박수영,황선갑,이동선,김종국,남주현,홍순덕,Park, Soo-Young,Hwang, Seon-Kap,Lee, Dong-Sun,Kim, Jong-Guk,Nam, Joo-Hyun,Hong, Soon-Duck 한국미생물 · 생명공학회 1995 한국미생물·생명공학회지 Vol.23 No.2

The alkaline phosphatase (K-ALPase) gene of Kluyveromyces fragilis has been cloned (1) and determined its base sequences (2) previously in our laboratory. When the K-ALPase gene was expressed in Escherichia coli and Saccharomyces cerevisiae, it showed a constitutive activity in E. coli, and a derepressed activity in S. cerevisiae in phosphate-limited medium. Northem hybridization experiment was performed to elucidate the transcription level of the K-ALPase gene. Northern experiment showed that transcription level of K-ALPase gene in S. cerevisiae was higher in phosphate depletion, but it was higher in high phosphate medium than in phosphate limited medium in K. fragilis. The transcription initiation site of the K-ALPase gene was determined by primer extension analysis. It matched nucleotide position - 169 in relation to the putative trnslational start site.

화재시 비차열 유리의 허용면적비에 관한 연구

박수영,서희원,왕남웅,김대회,여인환,최동호,Park. Soo Young,Seo. Hee Won,Wang. Nam Woong,Kim. Dae Hoi,Yeo. In Hwan,Choi. Dong Ho 한국방재학회 2014 한국방재학회논문집 Vol.14 No.2

국외에서는 용도 및 창의 크기를 제한하여 방화구획에 비차열 방화유리의 사용을 허용하는데 반해 국내에는 그 사용이 불가능하다. 따라서, 국내의 비차열 방화유리의 내화성능을 확인하고, 화재안전성을 평가해 볼 필요성이 있다. 본 연구에서는 비차열 방화유리에 대하여 면적별로 4가지 실험체를 구성하여 내화실험을 실시하고, 측정된 복사열유량 및 온도를 적용하여 화재안전성을 평가하였다. 그 결과 화재안전성을 확보하면서 사용이 가능한 면적비를 제안하였다. In foreign countries, a non-insulated fire glazed window is permitted in fire compartment restricting the use and size, but on the domestic side, not permitted. So, there is a need to confirm the fire resistance of the domestic non-insulated fire glazed window and to evaluate the fire safety of that. In this study, the fire resistance tests of 4 types of windows were conducted depending on window area, and the measured radiant heat fluxes and temperatures were evaluated in fire safety. As a result, we suggested the window/wall area ratio secured the fire safety.

지방행정과 특별자치단체

박수영 ( Soo Young Park ) 한국지방행정연구원 1989 地方行政硏究 Vol.4 No.3

금융기관종합보험상 종업원비행담보조항의 구조와 담보요건

박수영 ( Soo Young Park ) 한국상사판례학회 2011 상사판례연구 Vol.24 No.2

Fidelity bonds which are issued to banks are referred to as Banker`s Blanket Bonds, with blanket meaning it covers all employees of the insured bank(financial institution). The Banker`s Blanket Bonds indemnifies the insured against loss sustained by reason of specific perils described under eight different Insuring Agreements, which include: ① Fidelity, ② On Premises, ③ In Transit, ④ Forgery and Alteration, ⑤ Securities, ⑥ Counterfeited Currency, ⑦ Offices and Contents, and ⑧ Legal Fees. As fidelity bonds, Banker`s Blanket Bond is in fact a form of insurance. However, due to the risk that insured under the bond, it is not a liability insurance policy. A fidelity bond, including Banker`s Blanket Bond, is indemnity contracts that guarantee reimbursement for losses sustained by the insured resulting from the dishonesty or fraudulent actions of the insured`s institution`s employees and other specified perils. In other words, whereas liability insurance covers the liability of the insureds to a third-party, fidelity bonds cover the loss of property owned by the insureds or held by the insureds, as a result of employee dishonesty and other perils. The subject of coverage under a Banker`s Blanket Bond is dependant upon the terms of the multiple insuring agreements which may be contained therein. In general, unlike liability insurance, Banker`s Blanket Bonds do not cover the liability of the insureds to a third party. Instead, Banker`s Blanket Bonds provide coverage for loss of property which is owned by the insureds or held by the insureds, reimbursing the insured for such loss. Pursuant to the Fidelity insuring agreement, the Banker`s Blanket Bond is designed to guarantee reimbursement for losses sustained by the insured resulting from the dishonesty or fraudulent actions of the insured`s institution`s employees. Accordingly, in order to establish coverage under a fidelity bond, an insured must prove that its employees engaged in fraudulent or dishonest acts and that those acts in fact caused the subject loss being claimed by the insured. Fraudulent or dishonest acts generally mean acts committed by an employee with the manifest intent to cause the insured to sustain loss and to obtain financial gain for the employee other than those benefits earned during the normal course of employment. It is universally recognized that in order for there to be liability on the Banker`s Blanket Bond, there must be something more than negligence, mistake, carelessness, or incompetence. Accordingly, the Banker`s Blanket Bond does not insure good management nor does it insure against the risk of loss inherent in banking operations. The Banker`s Blanket Bond is likewise not intended to cover losses due to breach of contract.

조직배양 지치의 염색

박수영(Soo-Young Park),임형탁(Hyoung-Tak Im),황성진(Sung-Jin Hwang) 한국조형디자인학회 2005 조형디자인연구 Vol.8 No.1

본 연구는 식물염색의 생물 산업화에 있어서 기초가 되는 지속적 염료 공급의 가능성을 제시하기 위해 수행되었다. 생물 산업은 양질의 재료식물의 지속적 공급에 기반을 둔다. 전래의 중요한 염료식물인 지치는 과도한 채취로 절멸 위기에 처해 있으며 재배 또한 어려운 실정이다. 조직배양을 통해 증식시킨 모상근(hairy root)을 이용하여 지치의 알코올염색을 시도하였다. 1. 식물염색은 생물 산업으로 발전되어야 한다. 2. 생물 산업에는 고품질 식물재료의 지속적 공급이 필요하다. 3. 중요한 염료 및 약용자원식물인 지치는 희귀식물이 되었다. 4. 조직배양으로 지치를 대량 증식하고,알코올로 색소를 추출한다. 5. 공예와 생물학의 연결로 식물염색의 생물 산업화가 가능해진다. This study has been carried out to demonstrate the possibility of a sustainable supply of dyeing stuffs , which is the basis for industrialization of plant dyeing. The biotechnology industry is based onthe sustainable supply of quality raw plant s. Gromwell, one of the more important traditional dyes, is at risk of extinction due to heavy culling. Furthermore, its horticulture has turned out to be very difficult. This study tries to alcohol(ETOH extracting gromwell dye from hairy roots cultivated through tissue culture. 1. Plant dyeing should be developed into an industry. 2. The biotechnology industry requires a sustainable supply of quality raw plants. 3. Gromwell, an important dyes and medicinal plant, has become an endangered species . 4. Dye is extracted from gromwell using ETOH, which is cultivated on a large scale through tissue culture. 5. Linkage of cut craft with biology enables the industrialization of plant dyeing.