세균속간 시데로포아 생산능의 비교에 Chrome azurol S 한천배지 확산법의 응용

배영훈,박라영,신성희 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

연구배경 : 세균이 생산하는 시데로포아를 정량적으로 측정하기 위한 새로운 측정법인 chrome azurol S 한천배지 확산법(CASAD)이 저자들에 의해 개발되었다. 본 연구에서는 이 CASAD 법이 세균속간 시데로포아 생산을 비교하는 데에도 사용될 수 있는지를 알아보고자 하였다. 방법: Staphvlococcus aureus, Staphylococcus eptdermidis, Bacillus cereus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Vibrio vulnificus 균을 철을 제거한배지에 트렌스페린을 철공급원으로 공급한 배지에서 배양하면서 세균들의 증식은 파장 600 nm에서 흡광도를 측정하여 관찰하고 시데로포아 생산능은 CASAD법을 이용하여 관찰하였다. 트렌스페린으로부터 철을 획득하는 능력을비교하기 위하여 6 M urea를 함유하는 전기영동법을 사용하였다. 결과: 세균속간 시데로포아 생산능에는 큰 차이가 있었다. S. aureus, S. epidermidis, E. coli, P. aeruginosa는 B. cereus, B. subtilis, V. vulnificus체 비해 더 많은 양의 시데로포아를 생산할 수 있었고 트렌스페린으로부터 철을 더 효율적으로 획득할 수 있었으며 트렌스페린이 첨가된 배지에서 더 잘 자랄 수 있었다. 시데로포아 생산능과 트렌스페린으로부터 철을 획득하는 능력에는 밀접한 상관관계가 있었다. 결론: 본 연구를 통해 저자들은 CASAD법이 세균속간 시데로포아 생산능을 비교하는데에도 유용하게 사용될 수 있음을 알 수 있었다. Background: We recently developed a new chrome azurol S agar diffusion (CASAD) assay for the quantitative analysis of siderophore production. This study was designed to evaluate the usefulness of the CASAD assay for the comparison of the ability to produce siderophore among different genera of bacteria. Materials and Methods: Strains of Staphy lococctis aureus, Staphylococcus epidermidis, Bacillus cereus, Bacilhis subtilis, Escherichia coli, Pseudomonas aeruginosa, and Vibrio vulniftctis were used. The bacteria were cultured in deferrated heart infusion broth containing transferrin at 37℃ for 24 hours. During the cultures, Bacterial growth was measured by OD_(600), siderophore production was measured by CASAD assay, and iron-removal from transferrin was measured by 6 M urea-gel electrophoresis. Results: There was a big difference in the ability of the bacteria to produce siderophores. S. aureus, S. epidermidis, E. coli, and P. aeruginosa produced larger amounts of siderophores than B. cereus, B. subtilis, and V. vulnificus. S. aureus. S. epidermidis, E. coli, and P. aeruginosa utilized more efficiently transferrin-bound iron than B. cereus, B. subtilis, and V. vulnificus. The ability of the bacteria to produce siderophores was consistent with the ability of the bacteria to grow on transferrin-bound iron and to uptake iron from human transferrin. Conclusion: Through this study, we found that the CASAD assay is a useful universal method that can be applied for the intergeneric comparison of the ability to produce siderophores.

경험적 항응고요법과 ACT 측정에 따른 개별적 항응고요법이 혈액투석시 항응고 및 투석에 미치는 효과

허영숙,장인선,이명선,이유나,이형주,송지숙,권정아,이갑노,신진호,권영주,표희정 대한신장학회 2004 대한신장학회잡지 Vol.23 No.2

유지 혈액투석 환자에서 에리트로포에틴의 반응성에 관한 연구

이지은,김정엽,고강지,김명규,김희영,신진호,권영주,표희정,오경식 대한신장학회 2004 대한신장학회잡지 Vol.23 No.1

Inactivation of <i>Vibrio vulnificus</i> Hemolysin by Oligomerization but Not Proteolysis

Shin, Sung-Heui,Sun, Hui-Yu,Choi, Mi-Hwa,Park, Ra-Young,Bai, Young-Hoon,Kim, Choon-Mee,Kim, Soo-Young,Kim, Young-Ran,Lee, Shee-Eun,Rhee, Joon-Haeng Pharmaceutical Society of Japan 2005 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.28 No.7

<P><I>Vibrio vulnificus</I> extracellular protease (VvpE) is believed to destroy its hemolysin (VvhA) in the late growth phase, without obvious experimental evidence. So, we attempted to elucidate the mechanism. The hemolytic activity steeply increased with the expression of the VvhA in the early growth phase, and then abruptly declined with the expression of VvpE in the late growth phase. However, the VvhA activity also abruptly declined in a VvpE-deficient mutant. In Western blot, the degradation of VvhA was not observed; instead, the oligomerization of VvhA increased with the concomitant loss of hemolytic activity. These results evidently indicate that the inactivation of VvhA is due to the novel oligomerization of VvhA by unknown mechanism, but not to the destruction of VvhA by VvpE, so that the routine functional assay measuring hemolytic activity cannot reflect the actual production of VvhA.</P>

Urokinase-type plasminogen activator induces BV-2 microglial cell migration through activation of matrix metalloproteinase-9.

Shin, Sun Mi,Cho, Kyu Suk,Choi, Min Sik,Lee, Sung Hoon,Han, Seol-Heui,Kang, Young-Sun,Kim, Hee Jin,Cheong, Jae Hoon,Shin, Chan Young,Ko, Kwang Ho Kluwer Academic/Plenum Publishers 2010 Neurochem Res Vol.35 No.7

<P>In response to brain injury, microglia migrate and accumulate in the affected sites, which is an important step in the regulation of inflammation and neuronal degeneration/regeneration. In this study, we investigated the effect of urokinase-type plasminogen activator (uPA) on the BV-2 microglial cell migration. At resting state, BV-2 microglial cells secreted uPA and the release of uPA was increased by ATP, a chemoattractant released from injured neuron. The migration of BV-2 cell was significantly induced by uPA and inhibited by uPA inhibitors. In this condition, uPA increased the activity of matrix metalloproteinase (MMP-9) and the inhibition of MMP activity with pharmacological inhibitors against either uPA (amiloride) or MMP (phenanthrolene and SB-3CT) effectively prevented BV2 cell migration. Interestingly, the level of MMP-9 protein and mRNA in the cell were not changed by uPA. These results suggest that the increase of MMP-9 activity by uPA is regulated at the post-translational level, possibly via increased activation of the enzyme. Unlike the uPA inhibitor, plasmin inhibitor PAI-1 only partially inhibited uPA-induced cell migration and MMP-9 activation. The incubation of recombinant MMP-9 with uPA resulted in the activation of MMP-9. These results suggest that uPA plays a critical role in BV-2 microglial cell migration by activating pro-MMP-9, in part by its direct action on MMP-9 and also in part by the activation of plasminogen/plasmin cascade.</P>

<i>Vibrio vulnificus</i> metalloprotease VvpE has no direct effect on the iron-assimilation from human holotransferrin

Shin, Sung-Heui,Sun, Hui-Yu,Park, Ra-Young,Kim, Choon-Mee,Kim, Soo-Young,Rhee, Joon-Haeng Elsevier 2005 FEMS microbiology letters Vol.247 No.2

<P><B>Abstract</B></P><P>In order to elucidate the role of <I>Vibrio vulnificus</I> metalloprotease VvpE in the uptake of iron from human transferrin, we constructed a VvpE-deficient mutant and a merozygotic <I>vvpE</I>-transcriptional reporter from the wild type strain MO6-24/O. All three strains were able to grow only in deferrated Heart Infusion broth (DF-HI) with human holotransferrin (HT), but not in DF-HI containing partially iron-saturated transferrin or apotransferrin, without noticeable differences among the strains. All strains consumed most iron in the early growth phase. Both the transcription and extracellular production of VvpE proceeded at undetectable levels when bacterial growth was severely retarded in the DF-HI. When HT or FeCl<SUB>3</SUB> was added to the DF-HI, the retarded bacterial growth was restored and <I>vvpE</I> transcription dramatically increased in the late growth phase, but the extracellular VvpE production was negligible as compared to its transcription. All strains were unable to degrade HT even in normal HI broth containing HT, in which extracellular VvpE activity was remarkably high. The uptake of iron from HT in all strains was consistent with the production of catechol-siderophore rather than hydroxamate-siderophore. Similar results were also observed when clinical isolates from septicemic patients were used. In conclusion, we determined that VvpE was not directly involved in the siderophore-mediated iron-uptake from human transferrin. In addition, the discrepancy between the transcription and extracellular production of VvpE suggests that additional posttranscriptional events are involved in the extracellular production of VvpE.</P>

국내 상급종합병원과 종합병원에서의 정맥주입간호실무지침에 대한 태도와 확산정도

은영(Young Eun),구미옥(Mee Ok Gu),조용애(Young Ae Cho),김신미(Shin Mi Kim),정재심(Jae Shim Jeong),권정순(Jeong Soon Kwon),유정숙(Cheong Suk Yoo),정영선(Young Sun Jeong),정인숙(In Sook Jung),김경숙(Kyeong Sug Kim)이선희(Seon Heui Lee 한국근거기반간호학회 2014 근거와 간호 Vol.2 No.1

Purpose: This study was conducted to investigate the level of attitudes to and diffusion of Intravenous infusion nursing practice guideline in advanced general hospitals and general hospitals in Korea. Methods: The subjects were 41 nurses who were in charge of guideline diffusion in 41 advanced general hospitals and general hospitals. Data were collected between September 25 and November 2, 2014 by mail (return rates: 68.3 %). Data were analyzed using descriptive statistics, t-test, and ANOVA with SPSS/WIN 18.0. Results: The average attitude score of guideline among nurses in charge of guideline diffusion were 3.98±0.38 (range 1~5). The average guideline diffusion score and levels of diffusion was 2.26±1.39 (level of “persuasion of nursing knowledge”) for staff nurses, 3.05±0.86 (level of “use sometimes”) for nurses in charge of guideline diffusion, 2.87±0.78 (level of “use sometimes”) for nurse directors. 41.4 % of nursing divisions in 41 hospitals reviewed the intravenous infusion nursing practice guideline, educated the guideline to nurses, and implemented the guideline in clinical practice for guideline diffusion. Conclusion: Study findings indicate that nurses in charge of intravenous infusion nursing practice guideline in hospitals had positive attitude for guideline. The levels of guideline diffusion in hospitals was “persuasion of nursing knowledge” to “use sometimes”. Therefore, the strategies for promoting the diffusion and implementation of guideline in clinical practice in the level of nursing division is needed. Further study of outcome evaluation of guideline implementation will be needed.

광 CDMA와 WDM의 Hybrid를 위한 Decoder의 성능분석

신희영(Shin heui young),심형관(Sim hyuong kwan),유충현(Yoo chong hyun) 대한전기학회 2001 대한전기학회 학술대회 논문집 Vol.2001 No.7

말기 신부전 환자의 혈액투석에서 Tunneled Cuffed Catheter ( Permcath ) 사용 성적에 대한 연구

강영선(Young Sun Kang),이소영(So Young Lee),한상엽(Sang Yup Han),조상경(Sang Kyung Jo),신진호(Jin Ho Shin),차대룡(Dae Ryong Cha),권영주(Young Joo Kwon),조원용(Won Yong Cho),표희정(Heui Jung Pyo),김형규(Hyoung Kyu Kim) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.2

목적 : 혈액투석을 받는 만성 신부전 환자에게 혈관 접속장치의 확보는 환자의 생명을 유지시키기 위한 중요한 요소이다. 그러나 충분한 사전 준비 없이 시행되는 응급 혈액 투석시 흔히 사용되는 polyurethan teflon double lumen catheter는 비교적 사용기간이 짧으며, 사용 중 여러 가지 중한 합병증을 동반할 수 있다. 따라서 최근 피하에 매몰시킬 수 있는 dacron cuff를 갖고 있는 tunneled cuffed catheter(Permcath®)가 사용 중 합병증이 적고 충분한 기간 동안 사용할 수 있어 동정맥 문합 수술 후 혈관이 성숙되기까지 충분히 기다릴 수 있는 시간이 있고, 반 영구적인 목적으로 사용할 수 있다고 알려져 있다. 이에 이 연구에서는 tunneled cuffed catheter사용의 합병증 및 성적에 대해 조사하였다. 방법 : 1995년 1월부터 1999년 8월까지 환자 86명에 대하여 시행한 총 101예를 대상으로 조사하였으며 86명 중 27명은 다른 병원으로 전원하였다. 86명에 포함되지 않는 8명은 추적이 중단되어 조사대상에서 제외되었으며 전원된 환자는 병원이나 환자를 통한 전화 인터뷰를 시행하였다. 결과 : 전체 환자 86명의 평균 연령은 58세이고 남녀가 각각49명, 37명이었다. 이 중 35명은 당뇨병이 있었다. 대상환자 86명 중 4회 시행이 1명, 3회 시행이 1명, 2회가 10명이 있어 도관삽입은 101예에서 시행되었다. 사용 목적은 동정맥 문합술 후 혈관 성숙을 위해 대기하는 경우가 46%로 가장 많았고 혈관상태가 좋지 않아 혈관 수술을 할 수 없어 시행한 경우가 31%, 복막투석 중 합병증 발생의 경우가 15%였다. 혈관 수술을 위한 경우가 5%였으며 신장이식을 준비하는 경우도 있었다. 합병증은 시술환자 중 51%에서 발생하였으며, 이 중 국소 출혈이 70%로 많았으나 모든 예에서 도관관련 균혈증 가능성(possible catheter-related bacteremia, possible CRB)이 30%, 출구 감염이 14%, 기계적인 합병증이 7%를 차지했다. 카테터를 제거한 이유의 20%에서는 상기한 합병증 때문이었고 54%에서는 사용 목적이 사라져 의도적으로 제거한 경우였다. 내경 정맥 및 쇄골하정맥 등 카테터의 삽입 위치와 당뇨병 유무, 연령, 성별에 따른 전체 합병증의 차이는 없었다. 그리고 각각에 따른 출혈, 균혈증 등의 합병증에도 차이는 없었다. 그러나 출구 감염의 경우 쇄골하 정맥을 사용했을 때 더 흔히 발생하였다. 평균 사용기간은 4.5개월로 가장 긴 기간은 20개월이었다. 결론 : Tunneled cuffed catheter는 혈액투석을 필요로 하는 만성 신부전 환자에서 경한 합병증을 유발하면서 장기간 사용할 수 있어 장기적으로 유지투석을 시행해야 하는 만성 신부전 환자에서 일시적 또는 반영구적 혈관 접속장치로 유용할 것으로 생각되며, 향후 이의 적극적인 시도가 필요하리라 여겨진다. Background : Mortality and morbidity of ESRD patients depend on a maintenance of vascular access for dialysis. Polyurethane teflon double lumen catheters have been used for a temporary vascular access for hemodialysis. But, their use has a high rate of complications and a limited duration. Recently developed tunneled cuffed catheter(Permcath®) have lesser complications and longer durability and it could be an alternative for double lumen polyurethan catheter. This study evaluated the usefulness and complica- tions of a tunneled cuffed catheter as a long-term vascular access at the Korea University Medical Center. Methods: The study was done retrospectively through rnedical chart review and telephone interview to investigate age, sex, the absence or presence of diabetes mellitus, catheter performance, indications for a insertion, complications, causes of catheter removal. 101 catheters were inserted in 86 ESRD patients(80 jugular, 21 subclavian) from February, 1995 to August, 1999. The complication rates and it's association with diabetes mellitus and the location of catheter insertion were reviewed. Results: The rnean age of patients was 58 years (49 men, 37 women). 35 patients were diabetics. The purpose of catheters insertion were : 1) waiting for the maturation of an arteriovenous fistula(46%), 2) no other available vascular access(31%), 3) after a removal or insertion of peritoneal dialysis catheters (15%), 4) waiting for the better vascular access operation(5%). Catheters were removed because of mechanical obstructions(8%), infections(12%), deaths of patients(16%). 51% of patients had catheter related complications. Local bleeding(70%) was the most common complication. Bacteremia and exit site infection rate were 30% and 14% respectively. The complication rates had no association with age, sex, the absence or presence of diabetes mellitus and the location of catheter insertion. However, a subcalvian catheter was more vulnerable to exit site infection than an internal jugular catheter. Conclusions: A tunneled cuffed catheter is safer, and more durable than a polyurethane teflon double lumen catheter. It could be used for a short-term vascular access, or for a permanent vascular access in person otherwise with no other alternatives.

Utilization of Transferrin-Bound Iron by Medically Important Staphylococcal Species

Sung Heui Shin,Hui Yu Sun,Mi Hwa Choi,Young Hoon Bai,Ra Young Park 대한미생물학회 2005 Journal of Bacteriology and Virology Vol.35 No.2