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Proteomic analysis of extracellular vesicles derived from<i>Propionibacterium acnes</i>
Jeon, Jinseong,Mok, Hyuck Jun,Choi, Youngwoo,Park, Seung Cheol,Jo, Hunho,Her, Jin,Han, Jin-Kwan,Kim, Yoon-Keun,Kim, Kwang Pyo,Ban, Changill Wiley (John WileySons) 2017 PROTEOMICS CLINICAL APPLICATIONS Vol.11 No.1
각종 조혈모세포 근원별 CD34+ 양성세포율 및 혈구 집락배양의 비교
목지오,변재호,김숙자,전진우,원종호,백승호,서원석,홍대식,박희숙 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1
목적: 각종 조혈모세포의 근원인 말초혈액(PB), 고용량 항암요법과 조혈성장인자 투여후 얻은 말초혈액(MPB), 골수(BM) 및 제대혈 (UB)의 단핵구를 이용하여 CD₃₄+ 세포양성율 및 각종 조혈전구 세포의 집락 형성능을 비교 관찰하고자 한다. 방법: flow cytometry와 anti-CD₃₄+ 단세포항체를 이용하여 각종 조혈모세포 근원의 CD₃₄+ 양성세포함유 정도를 측정하였고, 분리된 단핵구를 집락배양하여 CFU-GM, BFU-E, CFU-GEMM 및 HPP-CFC의 집락형성능을 관찰하였다. 결과: 1) 각 조혈모세포의 근원별 CD₃₄+ 양성세포의 분리 전후의 비교 CD₃₄+ 양성세포의 양성률은 고용량 항암요법과 조혈성장인자 투여후 얻은 말초혈액에서 3.46±0.72%로 가장 높았으며 골수와 제대혈에서 각각 1.78±0.86%과 1.53±0.43%으로 비슷하였으며 말초혈액에서 0.14%로 가장 낮았다. CD₃₄+ 세포를 분리하였을 때, 분리후 CD₃₄+ 양성세포는 제대혈액에서 96.99±1.95%로 가장 높았으며 고용량 항암요법과 조혈성장인자 투여후 얻은 말초혈액에서 72.54±14.06%, 골수에서 60.6±1.9%, 그리고 말초혈액에서 54%로 나타났다. 2)각 조혈모세포의 근원별 조혈전구세포의 집락형성 CFU-GM, BFU-E, CFU-GEMM 그리고 HPP-CFC를 사용한 집락배양결과 CD₃₄+ 양성세포 분리군에서 비분리군에 비해 집락수가 많았으며, CFU-GM의 경우 그 비는 말초혈액, 골수, 그리고 제대혈액에서 각각 112배, 10배, 5배, 26배 또한 CFU-GEMM은 각각 136배, 8배, 12배가 높았다. 결론: 각종 조혈모세포의 근원별 CD₃₄+ 양성세포의 양성세포율 및 조혈전구세포 집락배양비교시 CD₃₄+ 양성세포와 조혈전구세포가 말초혈액이나 골수보다 MPB나 제대혈에서 훨씬 많은 것을 관찰하였다. Background: Classically bone marrow is the major source of hemopoietic stem cells of the allo/autologous hemopoietic stem cell tansplantation. Recently, hemopoietic stem cells circulate in peripheral blood, "mobilized" with cytokines and/or cytotoxic chemotherapy and umbilical cord blood stem cells, are widly used for high dose chemotherapy with hemopoietic stem cells support. The aim of this study is to investigate the possibility of clinical applications of selected CD34+ cells with source of hemopoietic stem cells such as, peripheral blood(PB), mobilized PB(MPB), bone marrow(BM), and umbilical cord blood(UB) Methods: We evaluated the comparison of the clonogenicithy and percentage of CD₃₄+ cells according to each source of hemopoietic stem cells in before and after separation of CD₃₄+ cells through in vitro colony assay and flow cytometry with anti-CD₃₄+ monoclonal antibody. Results: 1) CD34+ cells were detected in PB, MPB, BM, and UB at incidences of 0.14%, 3.46 ±0.72%, 1.78±0.86%, and 1.53±0.43% of total mononuclear cells. And the most CD34+ cells were detected in UB after seperation of CD₃₄+ cells. 2) The colony counts revealed more in CD₃₄+ isolating group. Clonogenicity of CD₃₄+ cells in PB, MPB, BM, and UB was 112-fold, 10-fold, 5-fold, and 26-fold in colony forming unit-granulocyte and macrophage(CFU-GM), and 136-fold, 8-fold, 3-fold, and 12-fold in colony of forming unit-granulocyte, erythrocyte, monocyte, megakaryocyte(CFU-GEMM). The colony counts of UB and MPB were large numbers more than PB and BM. Conclusion: Clonogenicity and percentage of CD₃₄+ cells are superior MPB and UB than in PB and BM.
이부프로펜 리지네이트의 직장흡수에 미치는 좌제기제 및 첨가제의 영향
이승목,최영욱,이정우,전홍렬,박동우 한국약제학회 1994 Journal of Pharmaceutical Investigation Vol.24 No.3
Ibuprofen is an effective non-steroidal anti-inflammatory drug (NSAID), but it has several limitations in clinical application because of low solubility in water and gastrointestinal irritation. A water-soluble salt of ibuprofen, ibuprofen lysinate, has been synthesized to overcome these shortcomings, and it was formulated as suppository for rectal administration. Witepsol and polyethylene glycols were employed as suppository bases for either ibuprofen or ibuprofen lysinate, in order to compare the bioavailability in rabbits. The plasma concentrations of ibuprofen were assayed by HPLC after a rectal administration of ibuprofen and ibuprofen lysinate, respectively. In addition to the comparison of two suppository bases, the other factors which affect on rectal absorption were also evaluated, especially in the point of not only particle size and shape of ibuprofen lysinate but also effects of additives such as stearic acid, cetyl alcohol and capric acid. And pharmacokinetic parameters such as AUC, C_(max), and t_(max) were also compared. In conclusion, spray-dried ibuprofen lysinate which was polyporous and spherical shape gave an increased absorption from the rectal formulations with Witepsol H15 and stearic acid.
박광현,Seung-II Jeong,Ji Ye Mok,Jung-Keun Cho,Ji Min Park,In Hwa Jeon,Hyeon Soo Kim,Seon Il Jang 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
In a previous study, our group showed that the effective microorganism substance (EM-S) produced by fermentation of medicinal plants with effective microorganisms has an antiinflammatory effect on atopic dermatitis-like lesions in NC/Nga mice. However, the possible antiinflammatory effect and skin barrier function of EM-S for inflammatory cell infiltration,Interleukin-4 (IL-4) production, C-C chemokine receptor 10 (CCR10), and filaggrin (FLG) expression were not reported. Therefore, effects of EM-S on the development of atopic dermatitislike skin lesions in NC/Nga mice were evaluated. Efficacy of EM-S was judged by measurement of scratching behavior, T-cell subset infiltration, cytokine production, and FLG expression. Topical application of EM-S significantly reduced scratching behavior in NC/Nga mice caused by house dust mite antigen (Dermatophagoides farinse extract, DfE) sensitization. IL-4 production and CD4+ and CD45+ cell infiltrations were significantly reduced by EM-S. CCR10 expression was also significantly inhibited by EM-S. EM-S treatment also increased the level of FLG reduced by DfE sensitization. These results demonstrate EM-S, when applied topically, may be an effective substance for management of atopic dermatitis patients.
들깨(Perilla frutescens) 새싹 추출물의 항산화 및 항염 효과
정승일(Seung-Il Jeong),김현수(Hyeon Soo Kim),전인화(In Hwa Jeon),강현주(Hyun Ju Kang),목지예(Ji Ye Mok),천춘진(Chun Jin Cheon),유현희(Hyeon Hee Yu),정선일(Seon Il Jang) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.1
본 연구는 들깨 새싹 추출물의 항산화, 항염증 및 항부종에 대한 효과를 조사하였다. 들깨 새싹 추출물은 DPPH와 ABTS 라디칼을 효과적으로 제거하는 항산화 활성이 우수하였다. 또한 들깨 새싹 추출물은 활성화된 설치류 유래 대식세포주인 RAW 264.7세포와 인간 유래 HMC-1 세포의 TNF-α와 IL-1β를 효과적으로 억제하였다. 더욱이 마우스의 귀와 발 부종을 억제하는 우수한 효과가 있었다. 이러한 결과는 들깨 새싹 추출물은 항산화제로 사용될 수 있을 뿐만 아니라 항염증과 항부종에 효과적인 물질이라는 것을 제시해주었다. 이와 관련된 들깨 새싹 추출물의 기능성에 대해서는 앞으로 분자생화학적 수준에서 더 연구해야할 필요성이 있는 것으로 사료된다. We investigated the effects of an ethanol extract from Perilla frutescens sprouts (PFSE) as an antioxidant, and its effects on edema and inflammation in RAW 264.7 cells and HMC-1 cells. The antioxidant activities (DPPH and ABTS radical scavenging) of PFSE were similar to those of butylated hydroxytoluene (BHT) and (±)-6-hydroxy-2,5,7,8- tetramethylchromane-2-carboxylic acid (Trolox). We also investigated the anti-inflammatory effects of PFSE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and HMC-1 cells stimulated with phorbol 12-myristate 13-acetate (PMA) with the calcium ionophore A23187. TNF-α and IL-1β production, which had been increased by treatment with LPS or PMA plus A23187, were significantly inhibited by PFSE in a dose-dependent manner. Furthermore, PFSE significantly reduced the xylene-induced ear edema and the carrageenan-induced paw edema of ICR mice in a dosedependent manner. The effects of PFSE (200 mg/kg) in reducing ear and paw edema were similar to those of aspirin (50mg/kg). These results suggest that PFSE can be potentially used as a medicine for treating oxidative stress, an edematous and inflammatory disease.