심초음파로 결정된 응급 심낭천자술의 천자부위

김성환,황성오,이강현,조준휘,강구현,문중범,이승환,윤정한,최경훈,김영식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: The aim of this study was to determine whether the conventional subcostal approach is suitable for emergency pericardiocentesis in patients with cardiac tamponade or impending cardiac tamponade. Methods: This study was a prospective, observational study conducted at the emergency department of a tertiary hospital, Patients who had symptomatic pericardial effusion and who needed emergency pericardiocentesis in the emergency department were included in this study. We measured the epicardium-to-pericardium distance at the subcostal, parasternal, and apical area with two-dimensional echocardiography to determine the appropriate puncture site for pericardiocentesis. An epicardium-to-pericardium distance of more than 1.0 cm was considered as the primary safety factor in determining the Puncture site for pericardiocentesis. The skin-to-pericardium distance was considered as secondary safety factor. Results: Ninety-five consecutive patients(55 males and 40 females; total mean age: 53 year old) with cardiac tamponade or impending cardiac tamponade were enrolled in this study. The puncture site for pericardiocentesis, as determined by echocardiography, was the subcostal area in 43 patients(45%), the apical area in 40 patients(42%), the left parasternal area In 11 patients(12%), and the right parastemal area in one patient(1%). Pericardiocentesis failed in 2 patients(2%) with the subcostal approach and in one patient(1%) with the apical approach. The average epicardium-to-pericardium distance was 31 ±21 mm in patients with the subcostal approach and 21±8 mm in patients with other approaches. There were no differences in the amount of pericardial fluid and in the intraperical pressure among patient groups according to puncture site. There were two procedure related complications: a puncture of the right ventricle with the subcostal approach and a ventricular tachycardia with the apical approach.

이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향

황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.

비가족성 양성 재발성 간내 담즙울체증 1 예

김오영,김미경,김주호,성범용,오현택,손현이,이광웅,김형권,윤해성,공규돈,신용민 대한간학회 1998 Clinical and Molecular Hepatology(대한간학회지) Vol.4 No.2

Benign recurrent intrahepatic cholestasis (BRIC) is a rare desease, which usually manifests between the age of 10 and 20. Its main clinical feature is multiple recurrent episodes of cholestasis without extrahepatic bile duct obstruction. We report here a case of nonfamilial benign recurrent intrahepatic cholestasis. The patient has experienced recurrent jaundice with pruritus since childhood. Main bile duct obstrution was excluded by abdominal CT and endoscopic retrograde cholangiopancreatography. Other causes of cholestasis were not found. Hepatic histology revealed bile plug which were mainly concentrated in the centrilobular region, and increased number of mononuclear cells in the portal triad, but hepatic parenchyma showed no inflammation and necrosis. In the last anicteric period, she was healthy and the liver function test and biopsy specimen were normal. (Korean J Hepatol 1998;4:188 - 193)

Expression of Sonic hedgehog signaling molecules in normal, hyperplastic and carcinomatous endometrium

Kim, Kyung Hee,Kim, Jin Man,Choi, Yoon-La,Shin, Young Kee,Lee, Ho-chang,Seong, In Ock,Kim, Bum Kyung,Chae, Seoung Wan,Chung, Yun-Shin,Kim, Seok-Hyung Blackwell Publishing Asia 2009 Pathology international Vol.59 No.5

<P>The aim of the present study was to determine the expression profile of the hedgehog (Hh) signaling molecules in normal, hyperplastic, and carcinomatous uterine endometrium. For this purpose, 271 endometrial tissue samples, (62 of normal endometrium, 127 of endometrial hyperplasias, and 82 endometrial adenocarcinomas) were studied using antibodies recognizing Hh-related signaling proteins, such as, sonic hedgehog (Shh), Patched (PTCH), Smoothened (Smo), Suppressor of fused [Su(Fu)], Gli-1, Gli-2, and Gli-3 by immunohistochemistry. The mRNA expression of these molecules was also assessed on reverse transcription–polymerase chain reaction. In the normal endometrium, the expression of Hh signaling molecules was generally downregulated except for Su(Fu), Gli-2, and Shh. In particular, the expression of both PTCH and Smo was very low or almost absent. Overall expression of Hh signaling molecules increased in hyperplastic endometrium; in particular, PTCH and Smo were significantly highly expressed in complex and atypical hyperplasia. In carcinoma samples extensive alterations were observed in the expression pattern of the signaling molecules. Nuclear Gli-2, cytoplasmic Gli-3, and Su(Fu) were overexpressed, whereas Shh, PTCH, and Smo expression were significantly reduced compared with the hyperplastic endometrium. The results suggest that the alteration of Hh signaling may be implicated in tumorigenesis of the endometrium.</P>

Identification of anti-adipogenic withanolides from the roots of Indian ginseng (Withania somnifera)

Seoung Rak Lee,Bum Soo Lee,Jae Sik Yu,강희선,Min Jeong Yoo,Sang Ah Yi,Jeung-Whan Han,Sil Kim,Jung Kyu Kim,Jin-Chul Kim,Ki Hyun Kim 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

Withania somnifera (Solanaceae), generally known as Indian ginseng, is a medicinal plantthat is used in Ayurvedic practice for promoting health and longevity. This study aims to identify thebioactive metabolites from Indian ginseng and elucidate their structures. Methods: Withanolides were purified by chromatographic techniques, including HPLC coupled with LC/MS. Chemical structures of isolated withanolides were clarified by analyzing the spectroscopic data from1D and 2D NMR, and HR-ESIMS experiment. Absolute configurations of the withanolides were established by the application of NMR chemical shifts and ECD calculations. Anti-adipogenic activities ofisolates were evaluated using 3T3-L1 preadipocytes with Oil Red O staining and quantitative real-timePCR (qPCR). Results: Phytochemical examination of the roots of Indian ginseng afforded to the isolation of sixwithanolides (1e6), including three novel withanolides, withasilolides GeI (1e3). All the six compoundsinhibited adipogenesis and suppressed the enlargement of lipid droplets, compared to those of thecontrol. Additionally, the mRNA expression levels of Fabp4 and Adipsin, the adipocyte markers decreasednoticeably following treatment with 25 mM of 1e6. The active compounds (1e6) also promoted lipidmetabolism by upregulating the expression of the lipolytic genes HSL and ATGL and downregulating theexpression of the lipogenic gene SREBP1. Conclusion: The results of our experimental studies suggest that the withasilolides identified herein haveanti-adipogenic potential and can be considered for the development of therapeutic strategies againstadipogenesis in obesity. Our study also provides a mechanistic rationale for using Indian ginseng as apotential therapeutic agent against obesity and related metabolic diseases.

2P-20 : Preparation and evaluation of sulfonated ion-exchange membranes using facilitated transport phenomena for diene separation

( Dong-bum Kim ),( Jong-ok Won ),( Yong-soo Kang ),( Seoung-hyun Kim ),( Hoon-sik Kim ) 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.-

Microstructure Study of Electrochemically Driven LixSi

Son, Seoung-Bum,Trevey, James E.,Roh, Hyunchul,Kim, Sung-Hwan,Kim, Kee-Bum,Cho, Jong Soo,Moon, Jeong-Tak,DeLuca, Christopher M.,Maute, Kurt K.,Dunn, Martin L.,Han, Heung Nam,Oh, Kyu Hwan,Lee, Se-Hee Wiley (John WileySons) 2011 Advanced energy materials Vol.1 No.6

AFC 기반 승·하차 게이트 탄력적 운영 방안 수립

김승범(Seoung-bum Kim),탁화정(Hwa-Jeong Tak),이종희(Jonghee Lee) 한국도시철도학회 2017 한국도시철도학회논문집 Vol.5 No.2

수도권 도시철도에서는 첨두시 이용자들이 집중됨에 따라 역사 내 승·하차 게이트에서 혼잡이 반복적으로 발생하고 있다. 이러한 게이트 지체는 승객들의 불편을 가중시키고 도시철도 효율성 및 서비스 질을 저하시키는 주된 요인 중 하나이다. 열차 추가투입 또는 게이트 추가설치와 같이 시설물의 양적 투입이 하나의 대안일 수 있지만 막대한 시간 및 비용투입이 필요한 만큼 비용·효율적으로 게이트 상의 혼잡을 완화시킬 수 있는 운영전략이 필요한 시점이다. 본 연구에서는 서울시 도심 내 주요 역사를 대상으로 AFC 데이터를 기반으로 추정된 지체도를 활용하여 게이트 상의 탄력적 게이트 운영(게이트 미터링) 방안 수립을 위한 기초자료를 제시하고자 한다. 먼저 분석 대상 역에서 승·하차 게이트의 시간별 지체도 추이를 분석하고, 지체 해소를 위해 필요한 추가게이트 수와 대향방향에서 전환 가능한 게이트 수를 비교하여 탄력적 운영 여부를 판단한다. 또한 탄력적 게이트 운영 가능 시간을 분석하여 실시간으로 변화하는 승객 수요에 민감한 탄력적 운영 전략 수립에 도움을 주고자 한다. 무엇보다 본 연구에서 제시하는 방법론은 추가적인 인프라 투입 없이 AFC 데이터만을 활용한다는 측면에서 공공목적으로서‘스마트카드 데이터 개방’과 같은 제반여건만 조성된다면 즉각적이고 지속적으로 적용할 수 있다는데 그 의의가 있다 Passenger in urban metho system often experiences delay at the alighting or boarding gate during peak periods. Gate delay would be one of major attributes to evaluate LOS of urban metro system. Although, there are only few efforts to quantify the measurement and implement it for alleviating gate delay. In this study, we seek to provide gate metering strategies to manage gate delay in cost-efficient way. This study employs smart card data from AFC system in the Seoul metro stations to estimate gate delay and develops baseline data that lead operators to make a decision for gate metering strategy.

Preparation and evaluation of sulfonated ion-exchange membranes using facilitated transport phenomena for diene separation

( Dong-bum Kim ),( Jong-ok Won ),( Yong-soo Kang ),( Seoung-hyun Kim ),( Hoon-sik Kim ) 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.1

Male-Specific W4P/R Mutation in the Pre-S1 Region of Hepatitis B Virus, Increasing the Risk of Progression of Liver Diseases in Chronic Patients

Lee, Seoung-Ae,Kim, Ki-Jeong,Kim, Dong-Won,Kim, Bum-Joon American Society for Microbiology 2013 Journal of clinical microbiology Vol.51 No.12

<P>The issue of hepatitis B virus (HBV) mutations possibly leading to a gender disparity in the progression of liver diseases has not been explored. We aimed to elucidate the relationships of the novel <I>pre-S1</I> mutations, W4P/R, with the progression of liver diseases and male predominance in a South Korean chronic cohort by use of a molecular epidemiologic study. We developed a fluorescence resonance energy transfer (FRET)-based real-time PCR (RT-PCR) assay for the detection of the W4P/R mutations and applied it to 292 chronic HBV patients. The <I>pre-S1</I> mutations from 247 (84.6%) of a total of 292 patients were detected by this assay. W4P/R mutants were found to be significantly related to severe liver diseases (hepatocellular carcinoma [HCC] and liver cirrhosis, 12.4% [19/153] of patients, versus chronic hepatitis and asymptomatic carriage, 1.1% [1/94] of patients) (<I>P</I> < 0.001). All of the W4P/R mutants were found in males only. The novel HBV <I>pre-S1</I> mutations, W4P/R, may be associated with disease severity in male patients chronically infected with HBV genotype C. The W4P/R mutations may provide in part an explanation for the relatively high ratio of male to female incidence in HCC generation in South Korean chronic HBV patients.</P>