A. 高校 科學 (生物·地球科學) 및 數學科 探究學習의 評價模型 定立과 評價紙 開發

李相協,權炳奎,林在圭,孫文求,吳岱燮,楊洪準,梁承榮,姜用熙,林成圭,金正遠,金吉民,兪正在 경북대학교 과학교육연구소 1982 科學敎育硏究誌 Vol.6 No.-

Currently the evaluation of high school science and mathematics learning has been too much knowledge-centered without concerning the inquiring ability or scientific attitude. This study, therefore, was made for the purpose of establishing of evaluation model for high school science (biology and earth science) and mathematics learning and its evaluation items. In this paper, the research was done about three fields, biology, earth science and mathematics. 1. For the biology, the evaluation methods of inquiry learning were studied with the foreign references, and proper evaluation items were suggested. 2. For the earth science, after analizing 5 kinds of earth science textbook of current using in high school, essential 19 items were selected. 5 of them were tested in test group and control group, then, the results were analized. 3. For the mathematice, the teaching elements of「Set」and「Flow chart」in mathematics (Ⅰ) were selected and structured. Detailed teaching objects were established and evaluation items were introduced.

骨格筋 S-Adenosyl-L-Methionine : Protein-Carboxyl O-Methyltransferase의 精製 및 特性 Protein-Carboxyl O-Methyltransferase from Bovine Skeletal Muscle

林圭,洪承元,郭相太,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

S-Adenosyl-L-methionine : protein-carboxyl 0-methyltransferase(prorein methylase II) has been purified from bovine skeletal muscle approximately 6,600-fold with a 7% yield by combination of ammonium sulfate precipitate, Sephadex G-75 chromatography, S-adenosyl-L-homocysteine-Sepharose 4B chromatography and hydroxyapatite chromatography, and then its characterizations and possible roles were investigated. The enzyme showed a optimum pH around 6.0. It was heat labile, inactivated by heat-treatment at 60℃ for 6 minutes, and when stored at -20℃ in the presence 10% glycerol, its activity has almost stabilized in 6 months. Copper ion(Cu^2+)and zinc ion(Zn^2+)were a potent inhibitors, the activity being completely inhibited at 0.25 mM and 1 mM respectively, The inhibition of copper and zinc ions were recovered 94%, 75% of its activity by adding 4 mM of EDTA, respectively. The apparent Km value for S-adenosyl--L-methionine was 2.86 x 10^-6 M. Kinetic analysis of enzyme in the presence of 30 uM copper ion showed that the nature of the inhibition to the enzyme was noncompetitive considering that Km was constant and Vmax was decreased. Histone IIA, immunoglobulin A and trypsin inhibitor were relatively good substrates for the enzyme. The enzyme activity was completely inhibited by 0.5 mM p-hydroxymercuribenzoic acid, but all of the activity was recovered in adding 10 mM mercaptoethanol, and the molecular weight of the enzyme was 24,000. Sarcolemma and sarcoplasmic reticulum of the skeletal muscle were good substrates for this enzyme and ^3H-CH_3 group was incorporated to 0.63 and 0.96 pmoles/mg protein/min, respectively. The carboxylmethylation of the sarcolemma of the skeletal muscle occurred in the 5 protein fractions(M. W. : 45,000, 22,000, below 14,400etc.) and that of sarcoplasmic reticulum occurred in the 7 protein fractions (M. W. : 100,000, 45,000, 22,000, below 14, 400 etc) by SDS/polyacrylamide gel electrophoresis. Among these molecular weight 22,000 was strongly methylated. These results discuss that protein methylase Il may play some roles in regulating physiological function of sarcolemma and sarcoplasmic reticulum of skeletal muscle.

高校 地球科學의 探究學習資料 開發과 그 適用에 關하여 : 지질도 작성 및 해석을 중심으로 especially on mapping and interpretation of the geological map

林成圭,韓承喜 경북대학교 과학교육연구소 1992 科學敎育硏究誌 Vol.16 No.-

The purpose of this study is to develop a inquiry learning instrument and to evaluate the effectiveness of experiment in the concept-forming about mapping and interpretation of the geological map. Unfortunately no proper experimental instruments on the content of this study has been utilized in the current domestic Earth Science textbooks. In this study, the geological structure models with 4 different dips were developed. In order to evaluate the effectiveness of the experiment, we prepared a test consisted of 10 problems and compared the test results performed by the control group who conducted the experiments along with regular class with another test results performed by comparative group who participated only regular class without experiment. The control group is consisted of 102 girls high school students in Taegu area, and the comparative group is 104 students. The test results are as follow : ⅰ)The mean of the test results of the control group (X_A) is 7.18, while that of comparative group(X_B) is 6.38. ⅱ)The difference of the test scores between two groups has been turned out to be highly significant at the 0.05 level of significance. (t=3.270 at the criterion for significance, α=0.05 and the degree of freedom, df=206). This suggest that the class learned by the experiment is more effective in the concept-forming of the making and interpretation of the geological map than the class only learned by lectures.

地球科學 敎科書 (人文系 高校)의 實驗學習內容의 分析

林成圭 경북대학교 과학교육연구소 1981 科學敎育硏究誌 Vol.5 No.-

The current Earth Science textbook in high school was revised in 1979 by the Ministry of Education. The purpose of this paper is to show the problem of the experiment contents in Earth Science textbook and to provide the reference data of better teaching for teachers. The results obtained in this study are summarized as follows:① The total number of experiment in 5 kinds of textbook is 97, but the number of common experiment is small. ② The distribution of the number of experiments is large in Unit Ⅰ & Ⅲ and amall in Unit Ⅳ & Ⅴ relatively. ③ 19 experiments which are considered very important in the current high school Earth Sciense course were selected and presented among out of 97 experiments.

유기성자원의 퇴비원료로 활용 가능성 평가방법 개발

임동규,이승환,성기석,소규호,신중두,이정택 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

우관상동맥 개구부 완전 폐쇄가 동반된 대동맥 판상부 협착증 1례

임상현,김종진,김희열,진승원,전희경,노태호,채장성,김재형,홍순조,최규보,윤정섭,김치경 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

韓國 白堊紀 慶尙層群의 恐龍足痕

林成圭 경북대학교 과학교육연구소 1988 科學敎育硏究誌 Vol.12 No.-

We observe the dinosaur footprints found in Dukmyeong-ri, Hai-myeon, Goseong-gun, Kyungsangnam-do, particularly the ones along the Sangzok coast in details. A total of 75 Trackways are identified in eight horizons: 63 thackways are for bipedal and the other 13 for quadrupedals The footprints of bipedal measure 22-37cm in length and 20-35cm in width, while the pes of quadrupedal are found to have the dimension of 24-38cm in length and 18-37cm width. The direction of tracks in six horizon shows a trend of nonrandoms.

결핵항원에 의한 결핵환자 말초혈액 단핵구의 IL-2,IL-10 및 TNF-α mRNA 발현비교

박정규,임영재,김화중,조은경,민들레,임재현,최덕례,박성규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

The various clinical features of tuberculosis are mediated by diverse cytokines produced by various immune cells which are initially triggered by M. tuberculosis antigens. CD4+ T cells can be classified into two subsets according to the patterns of cytokines they produce; Thl cells give rise to cell-mediated immunity and are characterized by the production of IL-2 and IFN-y, whereas Th2 cells are more efficient in mediating antibody production and secrete II-4, IL-5, IL-6 and II-10, Thl cells can control Th2 cell and vice versa. Peripheral blood mononuclear cells of healthy, cured and chronic refractory tuberculosis patients were stimulated with PPD, TSP and PHA antigen, and lymphoproliferative response and expression of II-2, IL- 10, TNF-α mRNAs were measured. Lymphoproliferative responses to PPD, TSP and PHA antigen were depressed in chronic refractory case compared with others expression of IL-2 mRNA was depressed in chronic refractory case stimulated with all antigens. Expression of IL-10 and TNF-α were depressed in cured and chronic refractory cases stimulated with PPD and TSP antigens.

특발성 경련질환으로 오인된 원발성 부갑상선기능저하증 1례

신성하,최정영,김준기,고영춘,조상욱,김순호,임중규 全南大醫大 2001 전남의대학술지 Vol.37 No.1

骨格筋 Protein Methylase Ⅱ에 關한 硏究

洪承元,郭相太,林圭,黃炳斗 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

Protein methylase Ⅱ (S-adenosy-L-methionine : protein-carboxyl 0-methyltransferase) has been, purifed 6,600-fold from bovine skeletal muscle and characterized. The purification procedures involved ammonium sulfate fractionation, Sephadex G-75 chromatography, S-adenosyl-L-homocysteine-Sepharose 4B chromatography, and hydroxyapatite chromatography. The enzyme showed an optimal pH around 6.0. It was heat-labile, being inactivated by heattreatment at 60℃ for 6 minutes. However, storage of the enzyme at -20℃ in the presence of 10% glycerol prevented the activity loss for 6 months. The apparent Km for S-adenosyl-L-methionine was 2.86 × 10 exp (6) M. Copper(Cu^2+) ion was potent noncompetitive inhibitor, the activity being completely inhibited b) 0.25 mM Cu^2+ and 1 mM Zn^2+. Most of the activity loss by copper and zinc ions were recovered by the addition of ethylenediamine tetracetate. The enzyme was also completely inhibited by 0.5 mM phydroxymercuribenzoate, which could be reversed by the addition of 10 mM 2-mercaptoethanol. The molecular weight of the enzyme was 24,000, as determined by Sephadex G-75 chromatography. Histone IIA, immunoglobulin A and soybean trypsin inhibitor were efficiently carboxylmethylated by the enzyme. Sarcoplasmic reticulum(SR) and sarcolemma(SL) of the skeletal muscle were also efficiently carboxylmethylated by the enzyme at a rate of 0.96 and 0.63 pmolesl mg protein/min of 3^H-CH_3, incorporation, respectively. Polyacrylamide gel electrophoresis of the carboxylmethylated SR and SL revealed multiple carboxylmethylated subfactions : SR resolved into the subfractions with molecular weight 100,000, 55,000, 22,000 and 14, 400, and SL into the subfractions with molecular weight 48,000, 22,000 and- 14, 400. Of these subfractions those with Mr=22, 000 were most strongly carboxylmethylated. Endogenous cAMP-dependent phoshorylation of SR occurred in the subfractions with Mr=55, 000, Mr=27, 000 and Mr=~14, 400, of which the fraction with Mr=27, 000 was most strongly phosphorylated. When SR was carboxylmethylated, the endogenous cAMP-dependent phosphorylation was decreased. SR exhibited relatively high Ca^2+-ATPase activity and oxalate-facilitated ATP-dependent 45^Ca^z+uptake. The carboxylmethylation and cAMP-dependent phosphorylation of SR increased the Ca^2+TATPase activity by 15% and by 29%, respectively. However, cAMP-dependent phosphorylation of SR which had been previously carboxylmethylated led to only 23% increase in the Ca^2+-ATPase activity. Both the carboxylmethylation and cAMP-dependent phosphorylation enhanced the steady-state rate as well as the initial rate of ^45Ca^2+-uptake. Kinetic analysis showed that the apparent Km and Vmax of Ca^2+ for ATP-dependent Ca^2+-uptake were 45μM and 35 nmoles/mg protein, respectively. Both the carboxylmethylation and cAMP-dependent phosphorylation of SR decreased the Km and increased the Vmax for the Ca^2+-uptake. These results suggest that protein methylase II might have some regulatory role in the calcium transport of sarcoplasmic reticulum.