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김은실,김종순,이명호,범희승,민정준,정환정,김성민,허영준,송호천,이제중 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.3
목적 : 림프구와 비교되는 수지상 세포의 방사선 민감성을 보기 위하여 본 연구를 시행하였다. 대상 및 방법 : 말초혈액에서 분리한 T 림프구에 0 Gy, 10 Gy, 30 Gy의 방사선을 조사하고 4시간 후에 유세포 분석기를 이용하여 선량별 세포고사 빈도를 관찰하였다. 또한 조혈모세포에서 미성숙 및 성숙 수지상 세포를 단계적으로 분리 배양하여 각각 0 Gy, 10 Gy, 30 Gy, 100 Gy의 방사선을 조사하고 4시간, 24시간 그리고 48 시간 후에 선량별 세포고사 빈도를 관찰하였다. 사이토스핀(cytospin)슬라이드에 림프구와 미성숙 및 성숙 수지상세포를 3×104개 씩 분주하고 May Grunwald-Giemsa 염색한 후, 광학 현미경 하에서 각각의 세포군 당 100개의 세포에서 세포 면적당 핵의 면적 비를 측정하였다. 결과 : 림프구에서는 방사선조사 선량별로 세포고사 빈도가 유의하게 증가하였으나, 수지상 세포에서는 그 분화정도나 방사선조사 선량에 따른 세포고사의 빈도차이가 없었다. 또한 수지상 세포는 방사선선량과 관계없이 용량에 의존적으로 강력한 T-세포 자극능을 보였다. 림프구의 세포에 대한 핵의 면적 비는 미성숙 및 성숙 수지상세포의 세포에 대한 핵의 면적 비보다 현저히 큰 반면, 두 가지 수지상세포간에 유의한 차이는 없었다. 결론 : 수지상세포는 그 분화도와 상관없이 림프구에 비하여 방사선 저항성을 나타내었고, 이는 세포의 형태적 차이에 따른 표적의 크기와 관련이 있을 것으로 생각되며, 향후 분자 생물학적인 연구의 기초 자료로 활용할 수 있을 것으로 생각된다. Purpose : To evaluate radiation sensitivity of dendritic cells in comparison with lymphocytes. Materials and methods : T lymphocytes captured from peripheral blood were irradiated by 0 Gy, 10 Gy, 30Gy. Apoptosis was measured by flowcytometry for staining of Annexin V 4 hours after irradiation. Immature and mature dendritic cells processed from blood hematopoietic stern cell were irradiated by 0 Gy, 10 Gy, 30 Gy, 100 Gy respectively and apoptosis was measured by flowcytometry with time difference as 4h, 24h and 48h after irradiation. Morphometric analysis by percent nucleus was measured in three cell groups, also. Results : Lymphocytes showed radiation sensitivity by increasing apoptotic fraction according to radiation dose. However, both mature and immature dendritic cells showed consistent fraction of apoptosis in spite of increasing radiation dose. Percent nucleus ratio is significantly higher in lymphocytes than that of mature or immature dendritic cells. Stimulation of T-cell by dendritic cells was not changed after irradiation. Conclusion : Dendritic cells showed radioresistance which was associated with small size of nucleus in comparison with lymphocytes and this result would be used as a basal data of radio-labelling for the cellular trafficking studies in nuclear medicine fields.
이은엽(Eun-Yup Lee),이영신(Young-Shin Lee),양추명(Qui-Ming Yang),김재훈(Jae-Hoon Kim),차기업(Ki-Up Cha),홍석균(Suk-Kyun Hong) 대한기계학회 2009 大韓機械學會論文集A Vol.33 No.4
Thick-walled cylinder with high pressure have had wide application in the armament industry. In the thick-walled cylinder, fatigue crack is generated at inner radius and developed toward the outer radius. To prevent generation of fatigue crack, the autofrettage process had been used. The compressive residual stress induced by the autofrettage process extends loading pressure and fatigue life of the thick-walled cylinder. In this study, the residual stress of single and compound cylinder by the autofrettage process was evaluated. The analytical compressive residual stress of single cylinder was good agreement with experimental result at inner radius. The analysis on the residual stress of compound cylinder was conducted. The compressive residual stress at inner radius was increased with the overstrain level. And fatigue life of the compound cylinder with initial crack was evaluated. The considered initial crack shape was straight and semi-elliptical. The fatigue life was extended with the overstrain level. The fatigue life of the compound cylinder with semi-elliptical crack was longer than straight crack. The suitable way to extend fatigue life of the compound cylinder was proposed.
Anoctamin 1/TMEM16A controls intestinal Cl− secretion induced by carbachol and cholera toxin
Byeongjun Lee,Gyu-Sang Hong,Sung Hoon LEE,Hyungsup Kim,Ajung Kim,Eun Mi Hwang,김지윤,Min Goo Lee,Jin-Young Yang,Mi-Na Kweon,Chung-Ming Tse,Donowitz Mark,Uhtaek Oh 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Calcium-activated chloride channels (CaCCs) mediate numerous physiological functions and are best known for the transport of electrolytes and water in epithelia. In the intestine, CaCC currents are considered necessary for the secretion of fluid to protect the intestinal epithelium. Although genetic ablation of ANO1/TMEM16A, a gene encoding a CaCC, reduces the carbachol-induced secretion of intestinal fluid, its mechanism of action is still unknown. Here, we confirm that ANO1 is essential for the secretion of intestinal fluid. Carbachol-induced transepithelial currents were reduced in the proximal colon of Ano1-deficient mice. Surprisingly, cholera toxin-induced and cAMP-induced fluid secretion, believed to be mediated by CFTR, were also significantly reduced in the intestine of Ano1-deficient mice. ANO1 is largely expressed in the apical membranes of intestines, as predicted for CaCCs. The Ano1-deficient colons became edematous under basal conditions and had a greater susceptibility to dextran sodium sulfate-induced colitis. However, Ano1 depletion failed to affect tumor development in a model of colorectal cancer. We thus conclude that ANO1 is necessary for cAMP- and carbachol-induced Cl− secretion in the intestine, which is essential for the protection of the intestinal epithelium from colitis.
Radical Scavenging Constituents of Cyperus flaccidus
Dalrae Ahn,Hyunjin Lee,Ming Ming Xing,Chao Tao,Eun Byeol Lee,Tae Gwan Lee,신태용,은재순,전훈,임종필,김대근 한국생약학회 2012 Natural Product Sciences Vol.18 No.2
In the course of screening for antioxidant compounds by measuring the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging effect and superoxide quenching activity, methanol extract of Cyperus flaccidus (Cyperaceae) was found to show potent antioxidant activities. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of ten compounds, kaempferol-3,7-O-α-L-dirhamnopyranoside (1),caffeic acid (2), quercetin-3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (3), kaempferol-3-O-α-Lrhamnopyranoside (4), quercetin-3-O-α-L-rhamnopyranoside (5), luteoiln-7-O-β-D-glucopyranoside (6), kaempferol-3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (7), luteoiln (8), quercetin (9) and quercetin-3-O-β-Dglucuronide (10). Their structures were elucidated by spectroscopic studies. Compounds 1 - 10 were isolated for the first time from this plant. Among them, compounds 2, 8 and 9 showed the significant radical scavenging effects on DPPH, and the potent xanthine-originated superoxide quenching activities.
Environmental estrogenic effects and gonadal development in wild goldfish (Carassius auratus)
Li, Chun-Ri,Lee, Sang-Hoon,Kim, So-Sun,Kim, Andre,Lee, Keun Woo,Lu, Ming,Kim, Hee-Eun,Kwak, Im-Jung,Lee, Yun-Ju,Kim, Dong-Kyoo,Lee, Jung-Sick,Kang, Shin-Won,Huh, Min-Do,Chung, Kyu-Hyuck,Park, Jang-Su Springer-Verlag 2009 Environmental monitoring and assessment Vol.150 No.1
Lee, Daewoo,Li, Ming-Yu,Sui, Mao,Zhang, Quanzhen,Pandey, Puran,Kim, Eun-Soo,Lee, Jihoon Springer US 2015 NANOSCALE RESEARCH LETTERS Vol.10 No.1
<P>Metallic nanoparticles have been widely witnessed in many applications: serving as the catalysts for various nanowire systems, as the active mediums of various device applications, and also for the nanoscale templates for hybrid quantum structures. In the performance of devices and configurations of the resulting nanostructures, the size and density of nanoparticles play critical roles. In this paper, the control of self-assembled Au droplets on GaAs (100), (110), and (111) is systematically investigated through the variation of deposition amount (DA), annealing temperature (AT), and dwelling time (DT). Based on the Volmer–Weber growth model, the formation of Au droplets and dramatic evolution of Au nanostructures on various GaAs surfaces is observed from the Au clusters to the round-dome shapes with the AT variation between 250 and 550 °C. With the systematic DA control, a radical size and density evolution of Au droplets shows the size expansion of over 400 % in average height and 800 % in average lateral diameter, while the density shows over two orders of decrease. With the DT variation, the self-assembled Au droplets tend to grow larger due to the Ostwald ripening while a clear distinction among the surface indexes is observed.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s11671-015-0950-z) contains supplementary material, which is available to authorized users.</P>
Ming-Jung Kang,Soo-Kyung Lee,Ji-Hyun Song,Mi-Eun Kim,Myo-Jeong Kim,Joung-Soon Jang,Jai-Hyun Lee,Jung-In Kim 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3
The α-glucosidase inhibitory and antioxidant effects of water chestnut (Trapa japonica Flerov.) were assessed to explore its possible use as an anti-diabetic agent. Methanol extracts of the fruit shell and meat of water chestnut were assayed for inhibitory activity against yeast α-glucosidase and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Effect of fruit shell extract on postprandial glucose response was assessed. Compared with fruit meat, shell extract showed stronger inhibition against α-glucosidase with an IC?? of 273 ㎍/㎖. Oral administration of fruit shell extract (500 ㎎/㎏) significantly lowered the postprandial area under the glucose response curve to starch (1 g/㎏) in streptozotocin (STZ)-induced diabetic rats (p<0.01). Compared with fruit meat, shell extract showed stronger scavenging activity against DPPH, with an IC?? of 27.1 ㎍/㎖. The results indicate that the fruit shell of water chestnut was effective in controlling postprandial hyperglycemia and exerted an antioxidant effect. Therefore, water chestnut may be useful in treating diabetes.